(Linear) (Six-base) MAPSORT of: KIBB5960.seq Check: 3299 from: 1 to: 1653 KIBB5960.orf With 182 enzymes: SgfI * October 7, 2009 14:18 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1080 1302 1653 Size: 1080 222 351 Fragments arranged by size: 1080 351 222 AatII G_ACGT'C Cuts at: 0 713 1653 Size: 713 940 AccI GT'mk_AC Cuts at: 0 4 1370 1653 Size: 4 1366 283 Fragments arranged by size: 1366 283 4 AclI AA'CG_TT Cuts at: 0 146 1653 Size: 146 1507 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 337 1653 Size: 337 1316 AflIII A'CryG_T Cuts at: 0 296 1653 Size: 296 1357 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1180 1653 Size: 1180 473 AlwNI CAG_nnn'CTG Cuts at: 0 315 490 730 838 943 1459 1653 Size: 315 175 240 108 105 516 194 Fragments arranged by size: 516 315 240 194 175 108 105 ApaI G_GGCC'C Cuts at: 0 103 760 962 1013 1355 1402 1591 1621 Size: 103 657 202 51 342 47 189 30 Cuts at: 1621 1653 Size: 32 Fragments arranged by size: 657 342 202 189 103 51 47 32 30 AvaI C'yCGr_G Cuts at: 0 511 1614 1653 Size: 511 1103 39 Fragments arranged by size: 1103 511 39 BamHI G'GATC_C Cuts at: 0 611 1653 Size: 611 1042 BanI G'GyrC_C Cuts at: 0 20 1215 1653 Size: 20 1195 438 Fragments arranged by size: 1195 438 20 BanII G_rGCy'C Cuts at: 0 103 499 760 962 1013 1127 1355 1402 Size: 103 396 261 202 51 114 228 47 Cuts at: 1402 1591 1621 1653 Size: 189 30 32 Fragments arranged by size: 396 261 228 202 189 114 103 51 47 32 30 BbeI G_GCGC'C Cuts at: 0 24 1653 Size: 24 1629 BbsI GAAGACnn'nnnn_ Cuts at: 0 705 1336 1653 Size: 705 631 317 Fragments arranged by size: 705 631 317 BbvCI CC'TCA_GC Cuts at: 0 1014 1271 1653 Size: 1014 257 382 Fragments arranged by size: 1014 382 257 BglI GCCn_nnn'nGGC Cuts at: 0 1397 1653 Size: 1397 256 BlpI GC'TnA_GC Cuts at: 0 380 1653 Size: 380 1273 Bme1580I G_kGCm'C Cuts at: 0 103 760 962 1013 1355 1402 1591 1621 Size: 103 657 202 51 342 47 189 30 Cuts at: 1621 1653 Size: 32 Fragments arranged by size: 657 342 202 189 103 51 47 32 30 BmrI ACTGGGnnnn_n' Cuts at: 0 680 1653 Size: 680 973 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 547 815 906 1216 1433 1571 1653 Size: 547 268 91 310 217 138 82 Fragments arranged by size: 547 310 268 217 138 91 82 Bpu10I CC'TnA_GC Cuts at: 0 498 1014 1271 1653 Size: 498 516 257 382 Fragments arranged by size: 516 498 382 257 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 36 427 1653 Size: 36 391 1226 Fragments arranged by size: 1226 391 36 BsaAI yAC'GTr Cuts at: 0 333 1565 1653 Size: 333 1232 88 Fragments arranged by size: 1232 333 88 BsaBI GATnn'nnATC Cuts at: 0 646 1653 Size: 646 1007 BsaHI Gr'CG_yC Cuts at: 0 21 213 710 1333 1653 Size: 21 192 497 623 320 Fragments arranged by size: 623 497 320 192 21 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 259 283 289 313 511 541 673 703 Size: 259 24 6 24 198 30 132 30 Cuts at: 703 1293 1323 1653 Size: 590 30 330 Fragments arranged by size: 590 330 259 198 132 30 30 30 24 24 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 562 565 604 619 667 697 1653 Size: 562 3 39 15 48 30 956 Fragments arranged by size: 956 562 48 39 30 15 3 BseYI C'CCAG_C Cuts at: 0 723 759 936 949 1081 1452 1653 Size: 723 36 177 13 132 371 201 Fragments arranged by size: 723 371 201 177 132 36 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 328 1653 Size: 328 1325 BsiEI CG_ry'CG Cuts at: 0 1396 1653 Size: 1396 257 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1531 1653 Size: 1531 122 Bsp1286I G_dGCh'C Cuts at: 0 103 499 760 962 1013 1127 1355 1402 Size: 103 396 261 202 51 114 228 47 Cuts at: 1402 1591 1621 1653 Size: 189 30 32 Fragments arranged by size: 396 261 228 202 189 114 103 51 47 32 30 BspHI T'CATG_A Cuts at: 0 167 1653 Size: 167 1486 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 638 1080 1302 1653 Size: 638 442 222 351 Fragments arranged by size: 638 442 351 222 BsrBI CCG'CTC Cuts at: 0 429 609 1653 Size: 429 180 1044 Fragments arranged by size: 1044 429 180 BsrDI GCAATG_nn' Cuts at: 0 93 1653 Size: 93 1560 BsrFI r'CCGG_y Cuts at: 0 259 413 867 1035 1218 1391 1653 Size: 259 154 454 168 183 173 262 Fragments arranged by size: 454 262 259 183 173 168 154 BssSI C'ACGA_G Cuts at: 0 81 1653 Size: 81 1572 BstEII G'GTnAC_C Cuts at: 0 1067 1653 Size: 1067 586 BstYI r'GATC_y Cuts at: 0 611 1053 1261 1653 Size: 611 442 208 392 Fragments arranged by size: 611 442 392 208 BtgI C'CryG_G Cuts at: 0 198 473 767 785 973 1653 Size: 198 275 294 18 188 680 Fragments arranged by size: 680 294 275 198 188 18 BtsI GCAGTG_nn' Cuts at: 0 289 1162 1653 Size: 289 873 491 Fragments arranged by size: 873 491 289 DrdI GACnn_nn'nnGTC Cuts at: 0 1338 1653 Size: 1338 315 EaeI y'GGCC_r Cuts at: 0 529 575 1393 1653 Size: 529 46 818 260 Fragments arranged by size: 818 529 260 46 EagI C'GGCC_G Cuts at: 0 1393 1653 Size: 1393 260 EarI CTCTTCn'nnn_ Cuts at: 0 419 1653 Size: 419 1234 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 73 532 1460 1653 Size: 73 459 928 193 Fragments arranged by size: 928 459 193 73 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 337 547 815 906 1216 1433 1571 1653 Size: 337 210 268 91 310 217 138 82 Fragments arranged by size: 337 310 268 217 210 138 91 82 EcoO109I rG'GnC_Cy Cuts at: 0 99 502 756 879 907 932 958 996 Size: 99 403 254 123 28 25 26 38 Cuts at: 996 1010 1351 1399 1587 1588 1618 1653 Size: 14 341 48 188 1 30 35 Fragments arranged by size: 403 341 254 188 123 99 48 38 35 30 28 26 25 14 1 HaeII r_GCGC'y Cuts at: 0 24 1558 1653 Size: 24 1534 95 Fragments arranged by size: 1534 95 24 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 259 291 673 705 1120 1152 1363 1395 Size: 259 32 382 32 415 32 211 32 Cuts at: 1395 1653 Size: 258 Fragments arranged by size: 415 382 259 258 211 32 32 32 32 HincII GTy'rAC Cuts at: 0 5 918 1371 1653 Size: 5 913 453 282 Fragments arranged by size: 913 453 282 5 KasI G'GCGC_C Cuts at: 0 20 1653 Size: 20 1633 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1451 1653 Size: 1451 202 MscI TGG'CCA Cuts at: 0 577 1653 Size: 577 1076 MslI CAynn'nnrTG Cuts at: 0 172 1653 Size: 172 1481 MspA1I CmG'CkG Cuts at: 0 387 490 542 567 835 949 1653 Size: 387 103 52 25 268 114 704 Fragments arranged by size: 704 387 268 114 103 52 25 NaeI GCC'GGC Cuts at: 0 869 1220 1393 1653 Size: 869 351 173 260 Fragments arranged by size: 869 351 260 173 NarI GG'CG_CC Cuts at: 0 21 1653 Size: 21 1632 NcoI C'CATG_G Cuts at: 0 198 767 973 1653 Size: 198 569 206 680 Fragments arranged by size: 680 569 206 198 NgoMIV G'CCGG_C Cuts at: 0 867 1218 1391 1653 Size: 867 351 173 262 Fragments arranged by size: 867 351 262 173 NspI r_CATG'y Cuts at: 0 300 1653 Size: 300 1353 PasI CC'CwG_GG Cuts at: 0 95 749 809 845 1058 1231 1653 Size: 95 654 60 36 213 173 422 Fragments arranged by size: 654 422 213 173 95 60 36 PciI A'CATG_T Cuts at: 0 296 1653 Size: 296 1357 PflMI CCAn_nnn'nTGG Cuts at: 0 333 1459 1653 Size: 333 1126 194 Fragments arranged by size: 1126 333 194 PfoI T'CCnGG_A Cuts at: 0 1174 1653 Size: 1174 479 PpuMI rG'GwC_Cy Cuts at: 0 879 907 932 996 1653 Size: 879 28 25 64 657 Fragments arranged by size: 879 657 64 28 25 PspOMI G'GGCC_C Cuts at: 0 99 756 958 1009 1351 1398 1587 1617 Size: 99 657 202 51 342 47 189 30 Cuts at: 1617 1653 Size: 36 Fragments arranged by size: 657 342 202 189 99 51 47 36 30 PstI C_TGCA'G Cuts at: 0 560 641 906 948 1299 1653 Size: 560 81 265 42 351 354 Fragments arranged by size: 560 354 351 265 81 42 PvuII CAG'CTG Cuts at: 0 387 490 567 835 949 1653 Size: 387 103 77 268 114 704 Fragments arranged by size: 704 387 268 114 103 77 SalI G'TCGA_C Cuts at: 0 3 1369 1653 Size: 3 1366 284 Fragments arranged by size: 1366 284 3 SanDI GG'GwC_CC Cuts at: 0 879 932 996 1653 Size: 879 53 64 657 Fragments arranged by size: 879 657 64 53 SapI GCTCTTCn'nnn_ Cuts at: 0 419 1653 Size: 419 1234 SbfI CC_TGCA'GG Cuts at: 0 906 1653 Size: 906 747 SfcI C'TryA_G Cuts at: 0 556 637 902 944 1295 1653 Size: 556 81 265 42 351 358 Fragments arranged by size: 556 358 351 265 81 42 SfoI GGC'GCC Cuts at: 0 22 1653 Size: 22 1631 SmaI CCC'GGG Cuts at: 0 1616 1653 Size: 1616 37 SmlI C'TyrA_G Cuts at: 0 15 442 1653 Size: 15 427 1211 Fragments arranged by size: 1211 427 15 StuI AGG'CCT Cuts at: 0 891 1423 1653 Size: 891 532 230 Fragments arranged by size: 891 532 230 StyI C'CwwG_G Cuts at: 0 198 449 767 922 973 1418 1578 1653 Size: 198 251 318 155 51 445 160 75 Fragments arranged by size: 445 318 251 198 160 155 75 51 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 780 1163 1575 1653 Size: 780 383 412 78 Fragments arranged by size: 780 412 383 78 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 511 543 954 986 1293 1325 1653 Size: 511 32 411 32 307 32 328 Fragments arranged by size: 511 411 328 307 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 9 1653 Size: 9 1644 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 237 456 1653 Size: 237 219 1197 Fragments arranged by size: 1197 237 219 XmaI C'CCGG_G Cuts at: 0 1614 1653 Size: 1614 39 XmnI GAAnn'nnTTC Cuts at: 0 322 1653 Size: 322 1331 ZraI GAC'GTC Cuts at: 0 711 1653 Size: 711 942 Enzymes that do cut: AarI AatII AccI AclI AcuI AflIII AlfI AlwNI ApaI AvaI BamHI BanI BanII BbeI BbsI BbvCI BglI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstEII BstYI BtgI BtsI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI PpuMI PspOMI PstI PvuII SalI SanDI SapI SbfI SfcI SfoI SmaI SmlI StuI StyI TaqII TstI Tth111I XcmI XmaI XmnI ZraI Enzymes that do not cut: Acc65I AfeI AflII AgeI AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BcgI BciVI BclI BfrBI BglII BmgBI BmtI BplI BsaI BsaWI BsiHKAI BsiWI BsmI BspEI BsrGI BssHII BstAPI BstBI BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI MfeI MluI NdeI NheI NotI NruI NsiI PacI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TatI XbaI XhoI