(Circular) (Six-base) MAPSORT of: pFN21AB5959.seq Check: 9599 from: 1 to: 6244 pFN21AB5959 6244 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2209 3385 2209 Size: 1176 5068 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2531 4054 278 Size: 53 83 186 936 995 1523 2468 Fragments arranged by size: 2468 1523 995 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3587 1937 Size: 1650 4594 Acc65I G'GTAC_C Cuts at: 3565 3565 Size: 6244 AclI AA'CG_TT Cuts at: 1563 4371 4744 1563 Size: 2808 373 3063 Fragments arranged by size: 3063 2808 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2198 3008 3337 4307 5691 5959 1632 Size: 566 810 329 970 1384 268 1917 Fragments arranged by size: 1917 1384 970 810 566 329 268 AfeI AGC'GCT Cuts at: 3533 3533 Size: 6244 AflII C'TTAA_G Cuts at: 820 1017 2189 820 Size: 197 1172 4875 Fragments arranged by size: 4875 1172 197 AflIII A'CryG_T Cuts at: 1203 2902 5143 6120 1203 Size: 1699 2241 977 1327 Fragments arranged by size: 2241 1699 1327 977 AgeI A'CCGG_T Cuts at: 5075 5075 Size: 6244 AhdI GACnn_n'nnGTC Cuts at: 4973 4973 Size: 6244 AleI CACnn'nnGTG Cuts at: 1166 2667 2978 1166 Size: 1501 311 4432 Fragments arranged by size: 4432 1501 311 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2891 1184 Size: 1707 4537 AlwNI CAG_nnn'CTG Cuts at: 2341 2434 3041 3197 3241 3377 3987 5559 2341 Size: 93 607 156 44 136 610 1572 3026 Fragments arranged by size: 3026 1572 610 607 156 136 93 44 ApaI G_GGCC'C Cuts at: 2088 2393 3421 2088 Size: 305 1028 4911 Fragments arranged by size: 4911 1028 305 ApaLI G'TGCA_C Cuts at: 2272 3499 4301 5457 2272 Size: 1227 802 1156 3059 Fragments arranged by size: 3059 1227 1156 802 ApoI r'AATT_y Cuts at: 1493 3553 3779 3931 6040 1493 Size: 2060 226 152 2109 1697 Fragments arranged by size: 2109 2060 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4798 160 Size: 872 3766 1606 Fragments arranged by size: 3766 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6244 AvaI C'yCGr_G Cuts at: 1870 1943 2055 2206 2564 3414 3441 3569 1870 Size: 73 112 151 358 850 27 128 4545 Fragments arranged by size: 4545 850 358 151 128 112 73 27 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2248 2281 2248 Size: 33 6211 BamHI G'GATC_C Cuts at: 3574 3941 3574 Size: 367 5877 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2404 3029 3428 3565 5020 Size: 325 217 627 617 625 399 137 1455 Cuts at: 5020 618 Size: 1842 Fragments arranged by size: 1842 1455 627 625 617 399 325 217 137 BanII G_rGCy'C Cuts at: 1391 2056 2088 2393 2421 2428 3181 3363 3421 Size: 665 32 305 28 7 753 182 58 Cuts at: 3421 3563 1391 Size: 142 4072 Fragments arranged by size: 4072 753 665 305 182 142 58 32 28 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5273 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4435 4469 1903 Size: 34 2498 34 3678 Fragments arranged by size: 3678 2498 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4136 5346 4136 Size: 1210 5034 BclI T'GATC_A Cuts at: 1548 2758 2941 1548 Size: 1210 183 4851 Fragments arranged by size: 4851 1210 183 BfrBI ATG'CAT Cuts at: 3230 3230 Size: 6244 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4855 136 Size: 107 122 71 631 605 3183 1525 Fragments arranged by size: 3183 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6239 6239 Size: 6244 BlpI GC'TnA_GC Cuts at: 3347 3661 3347 Size: 314 5930 Bme1580I G_kGCm'C Cuts at: 1790 2088 2276 2393 3421 3503 4305 5461 1790 Size: 298 188 117 1028 82 802 1156 2573 Fragments arranged by size: 2573 1156 1028 802 298 188 117 82 BmgBI CAC'GTC Cuts at: 1318 2905 1318 Size: 1587 4657 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2064 3029 3121 Size: 416 53 83 399 24 647 965 92 Cuts at: 3121 3254 3975 4933 442 Size: 133 721 958 1753 Fragments arranged by size: 1753 965 958 721 647 416 399 133 92 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6244 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2104 2104 Size: 6244 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2351 2435 2437 2686 4904 1239 Size: 1112 84 2 249 2218 2579 Fragments arranged by size: 2579 2218 1112 249 84 2 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4369 5234 5532 4369 Size: 865 298 5081 Fragments arranged by size: 5081 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2765 4907 882 Size: 617 1266 2142 2219 Fragments arranged by size: 2219 2142 1266 617 BsaAI yAC'GTr Cuts at: 493 1204 4035 493 Size: 711 2831 2702 Fragments arranged by size: 2831 2702 711 BsaBI GATnn'nnATC Cuts at: 6238 6238 Size: 6244 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2026 2528 4051 4433 Size: 53 83 186 936 493 502 1523 382 Cuts at: 4433 5068 275 Size: 635 1451 Fragments arranged by size: 1523 1451 936 635 502 493 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3328 3333 4677 5075 5349 5496 5907 3328 Size: 5 1344 398 274 147 411 3665 Fragments arranged by size: 3665 1344 411 398 274 147 5 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2043 2073 2354 2384 2665 Size: 30 165 30 640 30 281 30 281 Cuts at: 2665 2695 1178 Size: 30 4727 Fragments arranged by size: 4727 640 281 281 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2378 2972 1186 Size: 1192 594 4458 Fragments arranged by size: 4458 1192 594 BseYI C'CCAG_C Cuts at: 2133 2198 3074 3291 3363 5447 5943 2133 Size: 65 876 217 72 2084 496 2434 Fragments arranged by size: 2434 2084 876 496 217 72 65 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3002 3101 1163 Size: 573 1266 99 4306 Fragments arranged by size: 4306 1266 573 99 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3344 3694 4455 5483 1540 Size: 275 186 1343 350 761 1028 2301 Fragments arranged by size: 2301 1343 1028 761 350 275 186 BsiHKAI G_wGCw'C Cuts at: 2276 3486 3503 4305 4390 5461 2276 Size: 1210 17 802 85 1071 3059 Fragments arranged by size: 3059 1210 1071 802 85 17 BsmI GAATG_Cn' Cuts at: 3755 3848 3755 Size: 93 6151 BsmBI CGTCTCn'nnnn_ Cuts at: 2455 2455 Size: 6244 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2056 2088 2276 2393 2421 2428 3181 Size: 399 266 32 188 117 28 7 753 Cuts at: 3181 3363 3421 3486 3503 3563 4305 4390 5461 Size: 182 58 65 17 60 742 85 1071 Cuts at: 5461 1391 Size: 2174 Fragments arranged by size: 2174 1071 753 742 399 266 188 182 117 85 65 60 58 32 28 17 7 BspEI T'CCGG_A Cuts at: 3333 5907 3333 Size: 2574 3670 BspHI T'CATG_A Cuts at: 4133 5863 4133 Size: 1730 4514 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2209 2335 3385 3599 844 Size: 1365 126 1050 214 3489 Fragments arranged by size: 3489 1365 1050 214 126 BsrBI CCG'CTC Cuts at: 1394 1823 2129 4131 1394 Size: 429 306 2002 3507 Fragments arranged by size: 3507 2002 429 306 BsrDI GCAATG_nn' Cuts at: 65 1248 4739 4913 65 Size: 1183 3491 174 1396 Fragments arranged by size: 3491 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3339 3510 4888 5075 1700 Size: 110 1529 171 1378 187 2869 Fragments arranged by size: 2869 1529 1378 187 171 110 BsrGI T'GTAC_A Cuts at: 96 2551 2841 96 Size: 2455 290 3499 Fragments arranged by size: 3499 2455 290 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6244 BssSI C'ACGA_G Cuts at: 4304 5316 4304 Size: 1012 5232 BstAPI GCAn_nnn'nTGC Cuts at: 794 2341 3227 3507 794 Size: 1547 886 280 3531 Fragments arranged by size: 3531 1547 886 280 BstBI TT'CG_AA Cuts at: 5048 5048 Size: 6244 BstEII G'GTnAC_C Cuts at: 1184 2314 1184 Size: 1130 5114 BstXI CCAn_nnnn'nTGG Cuts at: 2728 2728 Size: 6244 BstYI r'GATC_y Cuts at: 1972 3255 3574 3941 4326 4343 5795 5881 5893 Size: 1283 319 367 385 17 1452 86 12 Cuts at: 5893 6239 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1283 385 367 346 319 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3172 3172 Size: 6244 BtgI C'CryG_G Cuts at: 513 1065 2004 2517 2609 513 Size: 552 939 513 92 4148 Fragments arranged by size: 4148 939 552 513 92 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3395 487 Size: 683 56 2169 3336 Fragments arranged by size: 3336 2169 683 56 BtsI GCAGTG_nn' Cuts at: 2111 2496 2683 3760 4554 4574 2111 Size: 385 187 1077 794 20 3781 Fragments arranged by size: 3781 1077 794 385 187 20 ClaI AT'CG_AT Cuts at: 1554 2463 1554 Size: 909 5335 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6209 DraI TTT'AAA Cuts at: 3548 3548 Size: 6244 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2533 5251 809 Size: 507 1217 2718 1802 Fragments arranged by size: 2718 1802 1217 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2514 2855 3691 Size: 54 1425 50 275 18 684 341 836 Cuts at: 3691 4580 6209 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 836 684 341 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3691 1537 Size: 275 1879 4090 Fragments arranged by size: 4090 1879 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3108 4174 1353 Size: 1755 1066 3423 Fragments arranged by size: 3423 1755 1066 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3298 4831 5205 5351 387 Size: 1064 138 1709 1533 374 146 1280 Fragments arranged by size: 1709 1533 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2198 2351 2435 2437 2686 3008 3337 Size: 393 566 153 84 2 249 322 329 Cuts at: 3337 4307 4904 5691 5959 1239 Size: 970 597 787 268 1524 Fragments arranged by size: 1524 970 787 597 566 393 329 322 268 249 153 84 2 EcoNI CCTnn'n_nnAGG Cuts at: 2216 2216 Size: 6244 EcoO109I rG'GnC_Cy Cuts at: 2066 2084 2085 2389 2390 2431 3201 2066 Size: 18 1 304 1 41 770 5109 Fragments arranged by size: 5109 770 304 41 18 1 1 EcoRI G'AATT_C Cuts at: 3553 3931 3553 Size: 378 5866 FspI TGC'GCA Cuts at: 4750 4750 Size: 6244 HaeII r_GCGC'y Cuts at: 1678 3535 5391 1678 Size: 1857 1856 2531 Fragments arranged by size: 2531 1857 1856 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2293 2325 3373 3405 4885 Size: 32 135 32 721 32 1048 32 1480 Cuts at: 4885 4917 4959 4991 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1480 1048 721 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3588 3834 669 Size: 988 281 1650 246 3079 Fragments arranged by size: 3079 1650 988 281 246 HindIII A'AGCT_T Cuts at: 748 3349 748 Size: 2601 3643 HpaI GTT'AAC Cuts at: 3834 3834 Size: 6244 KpnI G_GTAC'C Cuts at: 3569 3569 Size: 6244 MfeI C'AATT_G Cuts at: 3843 3843 Size: 6244 MluI A'CGCG_T Cuts at: 6120 6120 Size: 6244 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2463 5358 5542 1305 Size: 1158 2895 184 2007 Fragments arranged by size: 2895 2007 1158 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2857 10 Size: 54 1425 343 1025 3397 Fragments arranged by size: 3397 1425 1025 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2667 2978 4202 4561 4720 518 Size: 648 1501 311 1224 359 159 2042 Fragments arranged by size: 2042 1501 1224 648 359 311 159 MspA1I CmG'CkG Cuts at: 1606 2298 2640 2658 2986 3291 3660 4337 5485 Size: 692 342 18 328 305 369 677 1148 Cuts at: 5485 5730 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 692 677 369 342 328 305 245 18 NcoI C'CATG_G Cuts at: 513 1065 2004 2517 2609 513 Size: 552 939 513 92 4148 Fragments arranged by size: 4148 939 552 513 92 NdeI CA'TA_TG Cuts at: 387 387 Size: 6244 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6244 NotI GC'GGCC_GC Cuts at: 3691 3691 Size: 6244 NsiI A_TGCA'T Cuts at: 3232 3232 Size: 6244 NspI r_CATG'y Cuts at: 1132 1229 3602 5147 1132 Size: 97 2373 1545 2229 Fragments arranged by size: 2373 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3041 1348 Size: 1693 4551 PciI A'CATG_T Cuts at: 5143 5143 Size: 6244 PflMI CCAn_nnn'nTGG Cuts at: 1108 2434 2864 3041 1108 Size: 1326 430 177 4311 Fragments arranged by size: 4311 1326 430 177 PfoI T'CCnGG_A Cuts at: 2453 2453 Size: 6244 PmeI GTTT'AAAC Cuts at: 3548 3548 Size: 6244 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4313 4345 5826 5858 4313 Size: 32 1481 32 4699 Fragments arranged by size: 4699 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2066 3201 2066 Size: 1135 5109 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6244 PsiI TTA'TAA Cuts at: 3814 3814 Size: 6244 PspOMI G'GGCC_C Cuts at: 2084 2389 3417 2084 Size: 305 1028 4911 Fragments arranged by size: 4911 1028 305 PstI C_TGCA'G Cuts at: 830 2346 2357 2388 2543 3596 830 Size: 1516 11 31 155 1053 3478 Fragments arranged by size: 3478 1516 1053 155 31 11 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6244 PvuII CAG'CTG Cuts at: 2298 2986 3291 2298 Size: 688 305 5251 Fragments arranged by size: 5251 688 305 RsrII CG'GwC_CG Cuts at: 3331 3331 Size: 6244 SalI G'TCGA_C Cuts at: 1936 3586 1936 Size: 1650 4594 SanDI GG'GwC_CC Cuts at: 2066 2066 Size: 6244 SapI GCTCTTCn'nnn_ Cuts at: 3108 3108 Size: 6244 SbfI CC_TGCA'GG Cuts at: 3596 3596 Size: 6244 ScaI AGT'ACT Cuts at: 4492 6204 4492 Size: 1712 4532 SfcI C'TryA_G Cuts at: 826 1045 2342 2353 2384 2440 2539 3592 4727 Size: 219 1297 11 31 56 99 1053 1135 Cuts at: 4727 5408 5599 826 Size: 681 191 1471 Fragments arranged by size: 1471 1297 1135 1053 681 219 191 99 56 31 11 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6244 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6244 SmaI CCC'GGG Cuts at: 1872 2057 2566 3416 3571 1872 Size: 185 509 850 155 4545 Fragments arranged by size: 4545 850 509 185 155 SmlI C'TyrA_G Cuts at: 820 1017 1943 2189 4348 5249 5511 820 Size: 197 926 246 2159 901 262 1553 Fragments arranged by size: 2159 1553 926 901 262 246 197 SnaBI TAC'GTA Cuts at: 493 4035 493 Size: 3542 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6244 SphI G_CATG'C Cuts at: 1132 3602 1132 Size: 2470 3774 SspI AAT'ATT Cuts at: 5 52 4168 5 Size: 47 4116 2081 Fragments arranged by size: 4116 2081 47 StuI AGG'CCT Cuts at: 1517 3524 1517 Size: 2007 4237 StyI C'CwwG_G Cuts at: 513 1065 2004 2029 2178 2517 2609 3683 513 Size: 552 939 25 149 339 92 1074 3074 Fragments arranged by size: 3074 1074 939 552 339 149 92 25 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2585 2789 4271 4288 4441 4626 5071 Size: 1255 1005 204 1482 17 153 185 445 Cuts at: 5071 325 Size: 1498 Fragments arranged by size: 1498 1482 1255 1005 445 204 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2551 2841 4490 Size: 274 80 33 51 1443 574 290 1649 Cuts at: 4490 6202 96 Size: 1712 138 Fragments arranged by size: 1712 1649 1443 574 290 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2043 2075 2354 2386 2605 2637 2665 2697 3482 Size: 32 279 32 219 32 28 32 785 Cuts at: 3482 3514 6204 6236 2043 Size: 32 2690 32 2051 Fragments arranged by size: 2690 2051 785 279 219 32 32 32 32 32 32 28 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6244 XbaI T'CTAG_A Cuts at: 3580 3580 Size: 6244 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2759 2759 Size: 6244 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6244 XmaI C'CCGG_G Cuts at: 1870 2055 2564 3414 3569 1870 Size: 185 509 850 155 4545 Fragments arranged by size: 4545 850 509 185 155 XmnI GAAnn'nnTTC Cuts at: 4373 4373 Size: 6244 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2529 4052 276 Size: 53 83 186 936 995 1523 2468 Fragments arranged by size: 2468 1523 995 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BbeI BbvCI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI SacI SacII SexAI SfiI SfoI SrfI SwaI