Restriction map for
pFN21AB5927
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspHI AspI AvaI AviII
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglII BsaHI
BsaWI BseAI BsiHKAI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DraIII DrdI Eco47III
EcoO65I EcoRI FbaI FspI HaeII
Hin1I HindIII HpaI KasI KpnI
MfeI MluI MroI MunI NarI
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RsrII SacI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AhdI 1685 GACCCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 363 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1482 CCTAAGG CC^T[ACGT]AGG
ApaI 740 GGGCCC GGGCC^C
AspEI 1685 GACCCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 903 CCTAGG C^CTAGG
BglI 908 GCCAAAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 903 CCTAGG C^CTAGG
BlpI 1620 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1620 GCTGAGC GC^T[ACGT]AGC
BsaAI 1441 TACGTG [CT]AC^GT[GA]
BsaBI 592 GATATCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 971 GCATTC GAATGC(1/-1)
BstAPI 871 GCATTTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1482 CCTAAGG CC^T[ACGT]AGG
CelII 1620 GCTGAGC GC^T[ACGT]AGC
Eam1105I 1685 GACCCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1482 CCTAAGG CC^T[ACGT]AGG
EcoNI 96 CCTCTTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 592 GATATC GAT^ATC
EcoT22I 1502 ATGCAT ATGCA^T
FseI 59 GGCCGGCC GGCCGG^CC
HgaI 682 GACGC GACGC(5/10)
HincII 297 GTCGAC GT[CT]^[GA]AC
HindII 297 GTCGAC GT[CT]^[GA]AC
HphI 1697 TCACC GGTGA(8/7)
MamI 592 GATATCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 182 CCATGG C^CATGG
NdeI 815 CATATG CA^TATG
NsiI 1502 ATGCAT ATGCA^T
SalI 297 GTCGAC G^TCGAC
SfcI 1410 CTACAG C^T[GA][CT]AG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 297 GTCGAC GT^[AC][GT]AC
1408 GTCTAC
AfaI 207 GTAC GT^AC
1563 GTAC
BamHI 1314 GGATCC G^GATCC
1584 GGATCC
BcnI 18 CCCGG CC^[GC]GG
1226 CCCGG
BsaI 1075 GAGACC GGTCTC(1/5)
309 GGTCTC
BseRI 848 GAGGAG GAGGAG(10/8)
1011 GAGGAG
BspHI 1337 TCATGA T^CATGA
1662 TCATGA
BstYI 1314 GGATCC [GA]^GATC[CT]
1584 GGATCC
EagI 58 CGGCCG C^GGCCG
62 CGGCCG
EclXI 58 CGGCCG C^GGCCG
62 CGGCCG
Eco52I 58 CGGCCG C^GGCCG
62 CGGCCG
EcoT14I 182 CCATGG C^C[AT][AT]GG
903 CCTAGG
KspI 248 CCGCGG CCGC^GG
999 CCGCGG
MaeII 1122 ACGT A^CGT
1442 ACGT
MaeIII 1360 GTCAC ^GT[ACGT]AC
1465 GTGAC
MflI 1314 GGATCC [GA]^GATC[CT]
1584 GGATCC
MseI 407 TTAA T^TAA
602 TTAA
NaeI 60 GCCGGC GCC^GGC
64 GCCGGC
NciI 18 CCCGG CC^[GC]GG
1226 CCCGG
NgoMIV 60 GCCGGC G^CCGGC
64 GCCGGC
PvuII 338 CAGCTG CAG^CTG
1082 CAGCTG
RcaI 1337 TCATGA T^CATGA
1662 TCATGA
RsaI 207 GTAC GT^AC
1563 GTAC
SacII 248 CCGCGG CCGC^GG
999 CCGCGG
StyI 182 CCATGG C^C[AT][AT]GG
903 CCTAGG
Tru9I 407 TTAA T^TAA
602 TTAA
Tsp45I 1360 GTCAC ^GT[GC]AC
1465 GTGAC
XcmI 224 CCATCGAGATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1025 CCAGCATGGCCCTGG
XhoII 1314 GGATCC [GA]^GATC[CT]
1584 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AcsI AluI AlwI
ApoI AvaII BalI BanII BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaJI BsgI BsiEI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI Ksp632I
MaeI MboI MboII MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
PleI PpuMI Sau3AI Sau96I ScrFI
SfaNI TaqI TfiI TseI Tsp509I
TspRI TthHB8I