(Circular) (Six-base) MAPSORT of: pFN21AB5927.seq Check: 5856 from: 1 to: 6406 pFN21AB5927 6406 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2317 2317 Size: 6406 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4216 278 Size: 53 83 186 936 2680 2468 Fragments arranged by size: 2680 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2303 3414 3749 1937 Size: 366 1111 335 4594 Fragments arranged by size: 4594 1111 366 335 Acc65I G'GTAC_C Cuts at: 3727 3727 Size: 6406 AclI AA'CG_TT Cuts at: 1563 4533 4906 1563 Size: 2970 373 3063 Fragments arranged by size: 3063 2970 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2693 4469 5853 6121 1632 Size: 1061 1776 1384 268 1917 Fragments arranged by size: 1917 1776 1384 1061 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6209 AflIII A'CryG_T Cuts at: 1203 5305 6282 1203 Size: 4102 977 1327 Fragments arranged by size: 4102 1327 977 AgeI A'CCGG_T Cuts at: 5237 5237 Size: 6406 AhdI GACnn_n'nnGTC Cuts at: 3695 5135 3695 Size: 1440 4966 AleI CACnn'nnGTG Cuts at: 1166 2189 1166 Size: 1023 5383 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2141 1184 Size: 957 5449 AlwNI CAG_nnn'CTG Cuts at: 2373 4149 5721 2373 Size: 1776 1572 3058 Fragments arranged by size: 3058 1776 1572 ApaI G_GGCC'C Cuts at: 2749 2749 Size: 6406 ApaLI G'TGCA_C Cuts at: 4463 5619 4463 Size: 1156 5250 ApoI r'AATT_y Cuts at: 1493 2540 2558 3275 3715 3941 4093 6202 1493 Size: 1047 18 717 440 226 152 2109 1697 Fragments arranged by size: 2109 1697 1047 717 440 226 152 18 AseI AT'TA_AT Cuts at: 160 1032 4960 160 Size: 872 3928 1606 Fragments arranged by size: 3928 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6406 AvaI C'yCGr_G Cuts at: 1870 1943 3731 1870 Size: 73 1788 4545 Fragments arranged by size: 4545 1788 73 AvrII C'CTAG_G Cuts at: 2908 2908 Size: 6406 BamHI G'GATC_C Cuts at: 3319 3589 3736 4103 3319 Size: 270 147 367 5622 Fragments arranged by size: 5622 367 270 147 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3727 5182 618 Size: 325 217 627 1940 1455 1842 Fragments arranged by size: 1940 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2018 2749 2936 3632 3725 1391 Size: 627 731 187 696 93 4072 Fragments arranged by size: 4072 731 696 627 187 93 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2539 2790 3434 928 Size: 971 640 251 644 3900 Fragments arranged by size: 3900 971 644 640 251 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4597 4631 1903 Size: 34 2660 34 3678 Fragments arranged by size: 3678 2660 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4298 5508 4298 Size: 1210 5196 BclI T'GATC_A Cuts at: 1548 1548 Size: 6406 BfrBI ATG'CAT Cuts at: 3509 3509 Size: 6406 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2919 5017 136 Size: 107 122 71 631 605 1247 2098 1525 Fragments arranged by size: 2098 1525 1247 631 605 122 107 71 BglII A'GATC_T Cuts at: 6401 6401 Size: 6406 BlpI GC'TnA_GC Cuts at: 3626 3823 3626 Size: 197 6209 Bme1580I G_kGCm'C Cuts at: 1790 2040 2749 4467 5623 1790 Size: 250 709 1718 1156 2573 Fragments arranged by size: 2573 1718 1156 709 250 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6406 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2585 3277 4137 Size: 416 53 83 399 24 1168 692 860 Cuts at: 4137 5095 442 Size: 958 1753 Fragments arranged by size: 1753 1168 958 860 692 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6406 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2400 3374 3523 5066 1239 Size: 1161 974 149 1543 2579 Fragments arranged by size: 2579 1543 1161 974 149 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4531 5396 5694 4531 Size: 865 298 5243 Fragments arranged by size: 5243 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2320 3074 5069 882 Size: 617 821 754 1995 2219 Fragments arranged by size: 2219 1995 821 754 617 BsaAI yAC'GTr Cuts at: 493 1204 3448 4197 493 Size: 711 2244 749 2702 Fragments arranged by size: 2702 2244 749 711 BsaBI GATnn'nnATC Cuts at: 2601 6400 2601 Size: 3799 2607 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4213 4595 5230 275 Size: 53 83 186 936 2680 382 635 1451 Fragments arranged by size: 2680 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4839 5237 5511 5658 6069 4839 Size: 398 274 147 411 5176 Fragments arranged by size: 5176 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6181 Fragments arranged by size: 6181 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2868 3031 1186 Size: 1682 163 4561 Fragments arranged by size: 4561 1682 163 BseYI C'CCAG_C Cuts at: 2663 2740 2749 3085 3593 5609 6105 2663 Size: 77 9 336 508 2016 496 2964 Fragments arranged by size: 2964 2016 508 496 336 77 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2167 2264 2418 1163 Size: 573 431 97 154 5151 Fragments arranged by size: 5151 573 431 154 97 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2066 2070 2173 3856 4617 5645 Size: 275 186 65 4 103 1683 761 1028 Cuts at: 5645 1540 Size: 2301 Fragments arranged by size: 2301 1683 1028 761 275 186 103 65 4 BsiHKAI G_wGCw'C Cuts at: 4467 4552 5623 4467 Size: 85 1071 5250 Fragments arranged by size: 5250 1071 85 BsmI GAATG_Cn' Cuts at: 2976 3917 4010 2976 Size: 941 93 5372 Fragments arranged by size: 5372 941 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2018 2040 2749 2936 3632 3725 4467 Size: 399 228 22 709 187 696 93 742 Cuts at: 4467 4552 5623 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 709 696 399 228 187 93 85 22 BspEI T'CCGG_A Cuts at: 6069 6069 Size: 6406 BspHI T'CATG_A Cuts at: 3342 3667 4295 6025 3342 Size: 325 628 1730 3723 Fragments arranged by size: 3723 1730 628 325 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2133 2172 2199 2317 3288 3761 844 Size: 1289 39 27 118 971 473 3489 Fragments arranged by size: 3489 1289 971 473 118 39 27 BsrBI CCG'CTC Cuts at: 1394 1823 4293 1394 Size: 429 2470 3507 Fragments arranged by size: 3507 2470 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2697 2850 2955 4901 5075 65 Size: 1183 1449 153 105 1946 174 1396 Fragments arranged by size: 1946 1449 1396 1183 174 153 105 BsrFI r'CCGG_y Cuts at: 1700 1810 2065 2069 2096 5050 5237 1700 Size: 110 255 4 27 2954 187 2869 Fragments arranged by size: 2954 2869 255 187 110 27 4 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6406 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6406 BssSI C'ACGA_G Cuts at: 4466 5478 4466 Size: 1012 5394 BstAPI GCAn_nnn'nTGC Cuts at: 794 2882 794 Size: 2088 4318 BstBI TT'CG_AA Cuts at: 5210 5210 Size: 6406 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6406 BstXI CCAn_nnnn'nTGG Cuts at: 2194 2378 3093 2194 Size: 184 715 5507 Fragments arranged by size: 5507 715 184 BstYI r'GATC_y Cuts at: 1972 3319 3589 3736 4103 4488 4505 5957 6043 Size: 1347 270 147 367 385 17 1452 86 Cuts at: 6043 6055 6401 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1347 385 367 346 270 147 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3488 3488 Size: 6406 BtgI C'CryG_G Cuts at: 513 1065 2004 2187 2253 3004 513 Size: 552 939 183 66 751 3915 Fragments arranged by size: 3915 939 751 552 183 66 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3628 487 Size: 683 56 2402 3265 Fragments arranged by size: 3265 2402 683 56 BtsI GCAGTG_nn' Cuts at: 2371 3627 3922 4716 4736 2371 Size: 1256 295 794 20 4041 Fragments arranged by size: 4041 1256 794 295 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6406 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3121 3156 3275 3310 529 Size: 35 2557 35 119 35 3625 Fragments arranged by size: 3625 2557 119 35 35 35 DraI TTT'AAA Cuts at: 3710 3710 Size: 6406 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5413 809 Size: 507 4097 1802 Fragments arranged by size: 4097 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2063 2067 2226 Size: 54 1425 50 275 18 233 4 159 Cuts at: 2226 2250 2256 2949 3051 3280 3385 3598 3679 Size: 24 6 693 102 229 105 213 81 Cuts at: 3679 3853 4742 6371 8 Size: 174 889 1629 43 Fragments arranged by size: 1629 1425 889 693 275 233 229 213 174 159 105 102 81 54 50 43 24 18 6 4 EagI C'GGCC_G Cuts at: 1537 1812 2063 2067 3853 1537 Size: 275 251 4 1786 4090 Fragments arranged by size: 4090 1786 275 251 4 EarI CTCTTCn'nnn_ Cuts at: 1353 2108 2838 3141 4336 1353 Size: 755 730 303 1195 3423 Fragments arranged by size: 3423 1195 755 730 303 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4993 5367 5513 387 Size: 1064 138 3404 374 146 1280 Fragments arranged by size: 3404 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2400 2693 3374 3523 4469 5066 5853 Size: 393 768 293 681 149 946 597 787 Cuts at: 5853 6121 1239 Size: 268 1524 Fragments arranged by size: 1524 946 787 768 681 597 393 293 268 149 EcoNI CCTnn'n_nnAGG Cuts at: 2105 2105 Size: 6406 EcoO109I rG'GnC_Cy Cuts at: 2429 2708 2745 2746 3067 3349 3358 3484 3689 Size: 279 37 1 321 282 9 126 205 Cuts at: 3689 2429 Size: 5146 Fragments arranged by size: 5146 321 282 279 205 126 37 9 1 EcoRI G'AATT_C Cuts at: 3715 4093 3715 Size: 378 6028 EcoRV GAT'ATC Cuts at: 2599 2599 Size: 6406 FseI GG_CCGG'CC Cuts at: 2069 2069 Size: 6406 FspI TGC'GCA Cuts at: 4912 4912 Size: 6406 HaeII r_GCGC'y Cuts at: 1678 5553 1678 Size: 3875 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2432 2464 3681 3713 5047 Size: 32 135 32 860 32 1217 32 1334 Cuts at: 5047 5079 5121 5153 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1334 1217 860 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2304 3750 3996 669 Size: 988 281 366 1446 246 3079 Fragments arranged by size: 3079 1446 988 366 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6406 HpaI GTT'AAC Cuts at: 3996 3996 Size: 6406 KpnI G_GTAC'C Cuts at: 3731 3731 Size: 6406 MfeI C'AATT_G Cuts at: 4005 4005 Size: 6406 MluI A'CGCG_T Cuts at: 6282 6282 Size: 6406 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3297 5520 5704 1305 Size: 1992 2223 184 2007 Fragments arranged by size: 2223 2007 1992 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2951 3053 3387 10 Size: 54 1425 343 1119 102 334 3029 Fragments arranged by size: 3029 1425 1119 343 334 102 54 MslI CAynn'nnrTG Cuts at: 518 1166 2024 2189 2192 4364 4723 4882 518 Size: 648 858 165 3 2172 359 159 2042 Fragments arranged by size: 2172 2042 858 648 359 165 159 3 MspA1I CmG'CkG Cuts at: 1606 2062 2255 2345 3006 3089 3597 3822 4499 Size: 456 193 90 661 83 508 225 677 Cuts at: 4499 5647 5892 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 661 508 456 245 225 193 90 83 NaeI GCC'GGC Cuts at: 2067 2071 2067 Size: 4 6402 NcoI C'CATG_G Cuts at: 513 1065 2004 2187 513 Size: 552 939 183 4732 Fragments arranged by size: 4732 939 552 183 NdeI CA'TA_TG Cuts at: 387 2821 387 Size: 2434 3972 NgoMIV G'CCGG_C Cuts at: 2065 2069 2065 Size: 4 6402 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6406 NotI GC'GGCC_GC Cuts at: 3853 3853 Size: 6406 NsiI A_TGCA'T Cuts at: 3511 3511 Size: 6406 NspI r_CATG'y Cuts at: 1132 1229 3764 5309 1132 Size: 97 2535 1545 2229 Fragments arranged by size: 2535 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6406 PciI A'CATG_T Cuts at: 5305 5305 Size: 6406 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6406 PmeI GTTT'AAAC Cuts at: 3710 3710 Size: 6406 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4475 4507 5988 6020 4475 Size: 32 1481 32 4861 Fragments arranged by size: 4861 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2429 2708 3067 3349 3689 2429 Size: 279 359 282 340 5146 Fragments arranged by size: 5146 359 340 282 279 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6406 PsiI TTA'TAA Cuts at: 3976 3976 Size: 6406 PspOMI G'GGCC_C Cuts at: 2745 2745 Size: 6406 PstI C_TGCA'G Cuts at: 830 3758 830 Size: 2928 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6406 PvuII CAG'CTG Cuts at: 2345 3089 2345 Size: 744 5662 SacII CC_GC'GG Cuts at: 2256 3007 2256 Size: 751 5655 SalI G'TCGA_C Cuts at: 1936 2302 3748 1936 Size: 366 1446 4594 Fragments arranged by size: 4594 1446 366 SanDI GG'GwC_CC Cuts at: 3689 3689 Size: 6406 SbfI CC_TGCA'GG Cuts at: 3758 3758 Size: 6406 ScaI AGT'ACT Cuts at: 4654 6366 4654 Size: 1712 4694 SfcI C'TryA_G Cuts at: 826 1045 3415 3754 4889 5570 5761 826 Size: 219 2370 339 1135 681 191 1471 Fragments arranged by size: 2370 1471 1135 681 339 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6406 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6406 SmaI CCC'GGG Cuts at: 1872 3733 1872 Size: 1861 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4510 5411 5673 820 Size: 197 926 2567 901 262 1553 Fragments arranged by size: 2567 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4197 493 Size: 3704 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6406 SphI G_CATG'C Cuts at: 1132 3764 1132 Size: 2632 3774 SspI AAT'ATT Cuts at: 5 52 4330 5 Size: 47 4278 2081 Fragments arranged by size: 4278 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6406 StyI C'CwwG_G Cuts at: 513 1065 2004 2187 2908 3845 513 Size: 552 939 183 721 937 3074 Fragments arranged by size: 3074 939 937 721 552 183 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4433 4450 4603 4788 5233 325 Size: 1255 2853 17 153 185 445 1498 Fragments arranged by size: 2853 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4652 6364 96 Size: 274 80 33 51 1443 2675 1712 138 Fragments arranged by size: 2675 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2700 2732 3179 3211 3226 3258 6366 6398 2700 Size: 32 447 32 15 32 3108 32 2708 Fragments arranged by size: 3108 2708 447 32 32 32 32 15 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6406 XbaI T'CTAG_A Cuts at: 3742 3742 Size: 6406 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2236 3037 2236 Size: 801 5605 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6406 XmaI C'CCGG_G Cuts at: 1870 3731 1870 Size: 1861 4545 XmnI GAAnn'nnTTC Cuts at: 4535 4535 Size: 6406 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4214 276 Size: 53 83 186 936 2680 2468 Fragments arranged by size: 2680 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI FalI FspAI KasI NarI NruI PacI PfoI PmlI PspXI PsrI RsrII SacI SapI SexAI SfiI SfoI SrfI SwaI