(Circular) (Six-base) MAPSORT of: pFN21AB5912.seq Check: 9770 from: 1 to: 6292 pFN21AB5912 6292 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2564 2564 Size: 6292 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4102 278 Size: 53 83 186 936 2566 2468 Fragments arranged by size: 2566 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3635 1937 Size: 1698 4594 Acc65I G'GTAC_C Cuts at: 3613 3613 Size: 6292 AclI AA'CG_TT Cuts at: 1563 3519 4419 4792 1563 Size: 1956 900 373 3063 Fragments arranged by size: 3063 1956 900 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2168 3561 4355 5739 6007 1632 Size: 536 1393 794 1384 268 1917 Fragments arranged by size: 1917 1393 1384 794 536 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6095 AflIII A'CryG_T Cuts at: 1203 5191 6168 1203 Size: 3988 977 1327 Fragments arranged by size: 3988 1327 977 AgeI A'CCGG_T Cuts at: 5123 5123 Size: 6292 AhdI GACnn_n'nnGTC Cuts at: 5021 5021 Size: 6292 AleI CACnn'nnGTG Cuts at: 1166 2538 1166 Size: 1372 4920 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2833 1184 Size: 1649 4643 AlwNI CAG_nnn'CTG Cuts at: 2146 2651 4035 5607 2146 Size: 505 1384 1572 2831 Fragments arranged by size: 2831 1572 1384 505 ApaLI G'TGCA_C Cuts at: 4349 5505 4349 Size: 1156 5136 ApoI r'AATT_y Cuts at: 1493 2372 3601 3827 3979 6088 1493 Size: 879 1229 226 152 2109 1697 Fragments arranged by size: 2109 1697 1229 879 226 152 AseI AT'TA_AT Cuts at: 160 1032 4846 160 Size: 872 3814 1606 Fragments arranged by size: 3814 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6292 AvaI C'yCGr_G Cuts at: 1870 1943 3617 1870 Size: 73 1674 4545 Fragments arranged by size: 4545 1674 73 BamHI G'GATC_C Cuts at: 3622 3989 3622 Size: 367 5925 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2489 3613 5068 618 Size: 325 217 627 702 1124 1455 1842 Fragments arranged by size: 1842 1455 1124 702 627 325 217 BanII G_rGCy'C Cuts at: 1391 2560 3574 3611 1391 Size: 1169 1014 37 4072 Fragments arranged by size: 4072 1169 1014 37 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2036 928 Size: 971 137 5184 Fragments arranged by size: 5184 971 137 BbvCI CC'TCA_GC Cuts at: 2117 2117 Size: 6292 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4483 4517 1903 Size: 34 2546 34 3678 Fragments arranged by size: 3678 2546 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4184 5394 4184 Size: 1210 5082 BclI T'GATC_A Cuts at: 1548 1548 Size: 6292 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4903 136 Size: 107 122 71 631 605 3231 1525 Fragments arranged by size: 3231 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6287 6287 Size: 6292 BlpI GC'TnA_GC Cuts at: 3709 3709 Size: 6292 Bme1580I G_kGCm'C Cuts at: 1790 2222 2434 2892 4353 5509 1790 Size: 432 212 458 1461 1156 2573 Fragments arranged by size: 2573 1461 1156 458 432 212 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6292 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4023 4981 442 Size: 416 53 83 399 24 2606 958 1753 Fragments arranged by size: 2606 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6292 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4952 1239 Size: 3713 2579 Bpu10I CC'TnA_GC Cuts at: 2117 2117 Size: 6292 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2280 4417 5282 5580 2280 Size: 2137 865 298 2992 Fragments arranged by size: 2992 2137 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4955 882 Size: 617 3456 2219 Fragments arranged by size: 3456 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2684 3072 4083 493 Size: 711 1480 388 1011 2702 Fragments arranged by size: 2702 1480 1011 711 388 BsaBI GATnn'nnATC Cuts at: 6286 6286 Size: 6292 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2089 4099 4481 5116 Size: 53 83 186 936 556 2010 382 635 Cuts at: 5116 275 Size: 1451 Fragments arranged by size: 2010 1451 936 635 556 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4725 5123 5397 5544 5955 4725 Size: 398 274 147 411 5062 Fragments arranged by size: 5062 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6067 Fragments arranged by size: 6067 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2167 1186 Size: 981 5311 BseYI C'CCAG_C Cuts at: 2497 2820 3026 5495 5991 2497 Size: 323 206 2469 496 2798 Fragments arranged by size: 2798 2469 496 323 206 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2637 2715 1163 Size: 573 901 78 4740 Fragments arranged by size: 4740 901 573 78 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3100 3742 4503 5531 1540 Size: 275 186 1099 642 761 1028 2301 Fragments arranged by size: 2301 1099 1028 761 642 275 186 BsiHKAI G_wGCw'C Cuts at: 3013 4353 4438 5509 3013 Size: 1340 85 1071 3796 Fragments arranged by size: 3796 1340 1071 85 BsmI GAATG_Cn' Cuts at: 2026 2501 3803 3896 2026 Size: 475 1302 93 4422 Fragments arranged by size: 4422 1302 475 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2222 2434 2560 2892 3013 3574 3611 Size: 399 432 212 126 332 121 561 37 Cuts at: 3611 4353 4438 5509 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 561 432 399 332 212 126 121 85 37 BspEI T'CCGG_A Cuts at: 5955 5955 Size: 6292 BspHI T'CATG_A Cuts at: 2788 4181 5911 2788 Size: 1393 1730 3169 Fragments arranged by size: 3169 1730 1393 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2564 2825 3647 844 Size: 1720 261 822 3489 Fragments arranged by size: 3489 1720 822 261 BsrBI CCG'CTC Cuts at: 1394 1823 4179 1394 Size: 429 2356 3507 Fragments arranged by size: 3507 2356 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2275 2442 2568 4787 4961 65 Size: 1183 1027 167 126 2219 174 1396 Fragments arranged by size: 2219 1396 1183 1027 174 167 126 BsrFI r'CCGG_y Cuts at: 1700 1810 3095 4936 5123 1700 Size: 110 1285 1841 187 2869 Fragments arranged by size: 2869 1841 1285 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6292 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6292 BssSI C'ACGA_G Cuts at: 4352 5364 4352 Size: 1012 5280 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6292 BstBI TT'CG_AA Cuts at: 5096 5096 Size: 6292 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6292 BstXI CCAn_nnnn'nTGG Cuts at: 2476 2476 Size: 6292 BstYI r'GATC_y Cuts at: 1972 3622 3989 4374 4391 5843 5929 5941 6287 Size: 1650 367 385 17 1452 86 12 346 Cuts at: 6287 1972 Size: 1977 Fragments arranged by size: 1977 1650 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5740 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5553 Fragments arranged by size: 5553 683 56 BtsI GCAGTG_nn' Cuts at: 3808 4602 4622 3808 Size: 794 20 5478 Fragments arranged by size: 5478 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6292 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6257 DraI TTT'AAA Cuts at: 3596 3596 Size: 6292 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5299 809 Size: 507 3983 1802 Fragments arranged by size: 3983 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3097 3739 4628 Size: 54 1425 50 275 18 1267 642 889 Cuts at: 4628 6257 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1267 889 642 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3097 3739 1537 Size: 275 1285 642 4090 Fragments arranged by size: 4090 1285 642 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2144 3498 4222 1353 Size: 791 1354 724 3423 Fragments arranged by size: 3423 1354 791 724 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4879 5253 5399 387 Size: 1064 138 3290 374 146 1280 Fragments arranged by size: 3290 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2168 3561 4355 4952 5739 6007 1239 Size: 393 536 1393 794 597 787 268 1524 Fragments arranged by size: 1524 1393 794 787 597 536 393 268 EcoO109I rG'GnC_Cy Cuts at: 3579 3579 Size: 6292 EcoRI G'AATT_C Cuts at: 3601 3979 3601 Size: 378 5914 FspI TGC'GCA Cuts at: 4798 4798 Size: 6292 HaeII r_GCGC'y Cuts at: 1678 5439 1678 Size: 3761 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2478 2510 2645 2677 4933 Size: 32 135 32 906 32 135 32 2256 Cuts at: 4933 4965 5007 5039 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2256 906 135 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2623 3636 3882 669 Size: 988 281 685 1013 246 3079 Fragments arranged by size: 3079 1013 988 685 281 246 HindIII A'AGCT_T Cuts at: 748 2903 3472 748 Size: 2155 569 3568 Fragments arranged by size: 3568 2155 569 HpaI GTT'AAC Cuts at: 3882 3882 Size: 6292 KpnI G_GTAC'C Cuts at: 3617 3617 Size: 6292 MfeI C'AATT_G Cuts at: 2928 3891 2928 Size: 963 5329 MluI A'CGCG_T Cuts at: 6168 6168 Size: 6292 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5406 5590 1305 Size: 4101 184 2007 Fragments arranged by size: 4101 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4470 Fragments arranged by size: 4470 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2538 2790 2870 3444 4250 4609 4768 Size: 648 1372 252 80 574 806 359 159 Cuts at: 4768 518 Size: 2042 Fragments arranged by size: 2042 1372 806 648 574 359 252 159 80 MspA1I CmG'CkG Cuts at: 1606 2036 2194 3708 4385 5533 5778 1606 Size: 430 158 1514 677 1148 245 2120 Fragments arranged by size: 2120 1514 1148 677 430 245 158 NaeI GCC'GGC Cuts at: 3097 3097 Size: 6292 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5740 NdeI CA'TA_TG Cuts at: 387 387 Size: 6292 NgoMIV G'CCGG_C Cuts at: 3095 3095 Size: 6292 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6292 NotI GC'GGCC_GC Cuts at: 3739 3739 Size: 6292 NspI r_CATG'y Cuts at: 1132 1229 2673 3650 5195 1132 Size: 97 1444 977 1545 2229 Fragments arranged by size: 2229 1545 1444 977 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6292 PciI A'CATG_T Cuts at: 5191 5191 Size: 6292 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6292 PfoI T'CCnGG_A Cuts at: 3324 3324 Size: 6292 PmeI GTTT'AAAC Cuts at: 3596 3596 Size: 6292 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4361 4393 5874 5906 4361 Size: 32 1481 32 4747 Fragments arranged by size: 4747 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3579 3579 Size: 6292 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6292 PsiI TTA'TAA Cuts at: 3862 3862 Size: 6292 PstI C_TGCA'G Cuts at: 830 2199 3644 830 Size: 1369 1445 3478 Fragments arranged by size: 3478 1445 1369 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6292 PvuII CAG'CTG Cuts at: 2036 2036 Size: 6292 SalI G'TCGA_C Cuts at: 1936 3634 1936 Size: 1698 4594 SbfI CC_TGCA'GG Cuts at: 3644 3644 Size: 6292 ScaI AGT'ACT Cuts at: 4540 6252 4540 Size: 1712 4580 SfcI C'TryA_G Cuts at: 826 1045 2195 3495 3640 4775 5456 5647 826 Size: 219 1150 1300 145 1135 681 191 1471 Fragments arranged by size: 1471 1300 1150 1135 681 219 191 145 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6292 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6292 SmaI CCC'GGG Cuts at: 1872 3619 1872 Size: 1747 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2295 4396 5297 5559 820 Size: 197 926 352 2101 901 262 1553 Fragments arranged by size: 2101 1553 926 901 352 262 197 SnaBI TAC'GTA Cuts at: 493 2684 4083 493 Size: 2191 1399 2702 Fragments arranged by size: 2702 2191 1399 SpeI A'CTAG_T Cuts at: 152 152 Size: 6292 SphI G_CATG'C Cuts at: 1132 3650 1132 Size: 2518 3774 SspI AAT'ATT Cuts at: 5 52 2962 4216 5 Size: 47 2910 1254 2081 Fragments arranged by size: 2910 2081 1254 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6292 StyI C'CwwG_G Cuts at: 513 1065 2469 3731 513 Size: 552 1404 1262 3074 Fragments arranged by size: 3074 1404 1262 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4319 4336 4489 4674 5119 325 Size: 1255 2739 17 153 185 445 1498 Fragments arranged by size: 2739 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2075 4538 6250 Size: 274 80 33 51 1443 98 2463 1712 Cuts at: 6250 96 Size: 138 Fragments arranged by size: 2463 1712 1443 274 138 98 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3056 3088 3182 3214 6252 6284 3056 Size: 32 94 32 3038 32 3064 Fragments arranged by size: 3064 3038 94 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2246 3079 1316 Size: 930 833 4529 Fragments arranged by size: 4529 930 833 XbaI T'CTAG_A Cuts at: 3628 3628 Size: 6292 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3313 3563 3313 Size: 250 6042 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6292 XmaI C'CCGG_G Cuts at: 1870 3617 1870 Size: 1747 4545 XmnI GAAnn'nnTTC Cuts at: 3488 4421 3488 Size: 933 5359 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4100 276 Size: 53 83 186 936 2566 2468 Fragments arranged by size: 2566 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI