(Circular) (Six-base) MAPSORT of: pFN21AB5905.seq Check: 4368 from: 1 to: 6211 pFN21AB5905 6211 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4021 278 Size: 53 83 186 936 2485 2468 Fragments arranged by size: 2485 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3554 1937 Size: 1617 4594 Acc65I G'GTAC_C Cuts at: 3532 3532 Size: 6211 AclI AA'CG_TT Cuts at: 1563 4338 4711 1563 Size: 2775 373 3063 Fragments arranged by size: 3063 2775 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2897 2930 4274 5658 5926 1632 Size: 1265 33 1344 1384 268 1917 Fragments arranged by size: 1917 1384 1344 1265 268 33 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6014 AflIII A'CryG_T Cuts at: 1203 3201 5110 6087 1203 Size: 1998 1909 977 1327 Fragments arranged by size: 1998 1909 1327 977 AgeI A'CCGG_T Cuts at: 3369 5042 3369 Size: 1673 4538 AhdI GACnn_n'nnGTC Cuts at: 4940 4940 Size: 6211 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6211 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6211 AlwNI CAG_nnn'CTG Cuts at: 3954 5526 3954 Size: 1572 4639 ApaLI G'TGCA_C Cuts at: 4268 5424 4268 Size: 1156 5055 ApoI r'AATT_y Cuts at: 1493 3071 3520 3746 3898 6007 1493 Size: 1578 449 226 152 2109 1697 Fragments arranged by size: 2109 1697 1578 449 226 152 AseI AT'TA_AT Cuts at: 160 1032 4765 160 Size: 872 3733 1606 Fragments arranged by size: 3733 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6211 AvaI C'yCGr_G Cuts at: 1870 1943 3114 3536 1870 Size: 73 1171 422 4545 Fragments arranged by size: 4545 1171 422 73 AvrII C'CTAG_G Cuts at: 2218 2218 Size: 6211 BamHI G'GATC_C Cuts at: 3541 3908 3541 Size: 367 5844 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2039 3122 3532 4987 618 Size: 325 217 627 252 1083 410 1455 1842 Fragments arranged by size: 1842 1455 1083 627 410 325 252 217 BanII G_rGCy'C Cuts at: 1391 2561 3430 3530 1391 Size: 1170 869 100 4072 Fragments arranged by size: 4072 1170 869 100 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2130 928 Size: 971 231 5009 Fragments arranged by size: 5009 971 231 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4402 4436 1903 Size: 34 50 34 2381 34 3678 Fragments arranged by size: 3678 2381 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4103 5313 4103 Size: 1210 5001 BclI T'GATC_A Cuts at: 1548 1548 Size: 6211 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4822 136 Size: 107 122 71 631 605 3150 1525 Fragments arranged by size: 3150 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2998 6206 2998 Size: 3208 3003 BlpI GC'TnA_GC Cuts at: 2607 3628 2607 Size: 1021 5190 Bme1580I G_kGCm'C Cuts at: 1790 2042 3300 4272 5428 1790 Size: 252 1258 972 1156 2573 Fragments arranged by size: 2573 1258 1156 972 252 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6211 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2818 3294 3942 Size: 416 53 83 399 24 1401 476 648 Cuts at: 3942 4900 442 Size: 958 1753 Fragments arranged by size: 1753 1401 958 648 476 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6211 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2203 2203 Size: 6211 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2541 3126 4871 1239 Size: 1302 585 1745 2579 Fragments arranged by size: 2579 1745 1302 585 Bpu10I CC'TnA_GC Cuts at: 2560 2560 Size: 6211 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2955 4336 5201 5499 2955 Size: 1381 865 298 3667 Fragments arranged by size: 3667 1381 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4874 882 Size: 617 3375 2219 Fragments arranged by size: 3375 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4002 493 Size: 711 2798 2702 Fragments arranged by size: 2798 2702 711 BsaBI GATnn'nnATC Cuts at: 3247 6205 3247 Size: 2958 3253 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4018 4400 5035 275 Size: 53 83 186 936 2485 382 635 1451 Fragments arranged by size: 2485 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2448 3369 4644 5042 5316 5463 5874 2448 Size: 921 1275 398 274 147 411 2785 Fragments arranged by size: 2785 1275 921 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2320 2350 3246 3276 3320 Size: 30 165 30 917 30 896 30 44 Cuts at: 3320 3350 1178 Size: 30 4039 Fragments arranged by size: 4039 917 896 165 44 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6211 BseYI C'CCAG_C Cuts at: 3423 5414 5910 3423 Size: 1991 496 3724 Fragments arranged by size: 3724 1991 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2040 2275 1163 Size: 573 304 235 5099 Fragments arranged by size: 5099 573 304 235 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3661 4422 5450 1540 Size: 275 186 1660 761 1028 2301 Fragments arranged by size: 2301 1660 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2561 4272 4357 5428 2561 Size: 1711 85 1071 3344 Fragments arranged by size: 3344 1711 1071 85 BsmI GAATG_Cn' Cuts at: 2291 3425 3722 3815 2291 Size: 1134 297 93 4687 Fragments arranged by size: 4687 1134 297 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2042 2561 3300 3430 3530 4272 4357 Size: 399 252 519 739 130 100 742 85 Cuts at: 4357 5428 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 739 519 399 252 130 100 85 BspEI T'CCGG_A Cuts at: 5874 5874 Size: 6211 BspHI T'CATG_A Cuts at: 2848 3286 3411 4100 5830 2848 Size: 438 125 689 1730 3229 Fragments arranged by size: 3229 1730 689 438 125 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3566 844 Size: 2722 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4098 1394 Size: 429 2275 3507 Fragments arranged by size: 3507 2275 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2787 4706 4880 65 Size: 1183 1539 1919 174 1396 Fragments arranged by size: 1919 1539 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3369 4855 5042 1700 Size: 110 1559 1486 187 2869 Fragments arranged by size: 2869 1559 1486 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6211 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6211 BssSI C'ACGA_G Cuts at: 4271 5283 4271 Size: 1012 5199 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6211 BstBI TT'CG_AA Cuts at: 5015 5015 Size: 6211 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6211 BstXI CCAn_nnnn'nTGG Cuts at: 3372 3372 Size: 6211 BstYI r'GATC_y Cuts at: 1972 2998 3541 3908 4293 4310 5762 5848 5860 Size: 1026 543 367 385 17 1452 86 12 Cuts at: 5860 6206 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1026 543 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5659 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5472 Fragments arranged by size: 5472 683 56 BtsI GCAGTG_nn' Cuts at: 2330 3385 3727 4521 4541 2330 Size: 1055 342 794 20 4000 Fragments arranged by size: 4000 1055 794 342 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6211 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2096 2131 529 Size: 35 1532 35 4609 Fragments arranged by size: 4609 1532 35 35 DraI TTT'AAA Cuts at: 3515 3515 Size: 6211 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5218 809 Size: 507 3902 1802 Fragments arranged by size: 3902 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3658 4547 6176 Size: 54 1425 50 275 18 1828 889 1629 Cuts at: 6176 8 Size: 43 Fragments arranged by size: 1828 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3658 1537 Size: 275 1846 4090 Fragments arranged by size: 4090 1846 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2172 2929 4141 1353 Size: 819 757 1212 3423 Fragments arranged by size: 3423 1212 819 757 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4798 5172 5318 387 Size: 1064 138 3209 374 146 1280 Fragments arranged by size: 3209 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2559 2559 Size: 6211 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2541 2897 2930 3126 4274 4871 5658 Size: 393 909 356 33 196 1148 597 787 Cuts at: 5658 5926 1239 Size: 268 1524 Fragments arranged by size: 1524 1148 909 787 597 393 356 268 196 33 EcoNI CCTnn'n_nnAGG Cuts at: 3227 3227 Size: 6211 EcoO109I rG'GnC_Cy Cuts at: 2814 2814 Size: 6211 EcoRI G'AATT_C Cuts at: 3520 3898 3520 Size: 378 5833 EcoRV GAT'ATC Cuts at: 2800 2800 Size: 6211 FspI TGC'GCA Cuts at: 4717 4717 Size: 6211 HaeII r_GCGC'y Cuts at: 1678 5358 1678 Size: 3680 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2562 2594 2885 2917 4852 Size: 32 135 32 990 32 291 32 1935 Cuts at: 4852 4884 4926 4958 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1935 990 291 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2134 3097 3555 3801 669 Size: 988 281 196 963 458 246 3079 Fragments arranged by size: 3079 988 963 458 281 246 196 HindIII A'AGCT_T Cuts at: 748 748 Size: 6211 HpaI GTT'AAC Cuts at: 3801 3801 Size: 6211 KpnI G_GTAC'C Cuts at: 3536 3536 Size: 6211 MfeI C'AATT_G Cuts at: 2280 2985 3810 2280 Size: 705 825 4681 Fragments arranged by size: 4681 825 705 MluI A'CGCG_T Cuts at: 6087 6087 Size: 6211 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3132 5325 5509 1305 Size: 1827 2193 184 2007 Fragments arranged by size: 2193 2007 1827 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4389 Fragments arranged by size: 4389 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2853 3020 4169 4528 4687 518 Size: 648 1687 167 1149 359 159 2042 Fragments arranged by size: 2042 1687 1149 648 359 167 159 MspA1I CmG'CkG Cuts at: 1606 2724 3627 4304 5452 5697 1606 Size: 1118 903 677 1148 245 2120 Fragments arranged by size: 2120 1148 1118 903 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5659 NdeI CA'TA_TG Cuts at: 387 387 Size: 6211 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6211 NotI GC'GGCC_GC Cuts at: 3658 3658 Size: 6211 NspI r_CATG'y Cuts at: 1132 1229 3205 3569 5114 1132 Size: 97 1976 364 1545 2229 Fragments arranged by size: 2229 1976 1545 364 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6211 PciI A'CATG_T Cuts at: 3201 5110 3201 Size: 1909 4302 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6211 PfoI T'CCnGG_A Cuts at: 2518 2816 2518 Size: 298 5913 PmeI GTTT'AAAC Cuts at: 3515 3515 Size: 6211 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4280 4312 5793 5825 4280 Size: 32 1481 32 4666 Fragments arranged by size: 4666 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2814 2814 Size: 6211 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6211 PsiI TTA'TAA Cuts at: 3781 3781 Size: 6211 PspXI vC'TCGA_Gb Cuts at: 3114 3114 Size: 6211 PstI C_TGCA'G Cuts at: 830 2797 3563 830 Size: 1967 766 3478 Fragments arranged by size: 3478 1967 766 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6211 PvuII CAG'CTG Cuts at: 2724 2724 Size: 6211 SacI G_AGCT'C Cuts at: 2561 2561 Size: 6211 SalI G'TCGA_C Cuts at: 1936 3553 1936 Size: 1617 4594 SbfI CC_TGCA'GG Cuts at: 3563 3563 Size: 6211 ScaI AGT'ACT Cuts at: 2968 4459 6171 2968 Size: 1491 1712 3008 Fragments arranged by size: 3008 1712 1491 SfcI C'TryA_G Cuts at: 826 1045 2407 2601 2793 3559 4694 5375 5566 Size: 219 1362 194 192 766 1135 681 191 Cuts at: 5566 826 Size: 1471 Fragments arranged by size: 1471 1362 1135 766 681 219 194 192 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6211 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6211 SmaI CCC'GGG Cuts at: 1872 3538 1872 Size: 1666 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2970 3114 4315 5216 5478 820 Size: 197 926 1027 144 1201 901 262 1553 Fragments arranged by size: 1553 1201 1027 926 901 262 197 144 SnaBI TAC'GTA Cuts at: 493 4002 493 Size: 3509 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6211 SphI G_CATG'C Cuts at: 1132 3569 1132 Size: 2437 3774 SspI AAT'ATT Cuts at: 5 52 2140 4135 5 Size: 47 2088 1995 2081 Fragments arranged by size: 2088 2081 1995 47 StuI AGG'CCT Cuts at: 1517 2577 1517 Size: 1060 5151 StyI C'CwwG_G Cuts at: 513 1065 2218 3650 513 Size: 552 1153 1432 3074 Fragments arranged by size: 3074 1432 1153 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4238 4255 4408 4593 5038 325 Size: 1255 2658 17 153 185 445 1498 Fragments arranged by size: 2658 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2063 2966 4457 Size: 274 80 33 51 1443 86 903 1491 Cuts at: 4457 6169 96 Size: 1712 138 Fragments arranged by size: 1712 1491 1443 903 274 138 86 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2065 2097 2245 2277 3246 3278 3320 3352 6171 Size: 32 148 32 969 32 42 32 2819 Cuts at: 6171 6203 2065 Size: 32 2073 Fragments arranged by size: 2819 2073 969 148 42 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2129 2812 1316 Size: 813 683 4715 Fragments arranged by size: 4715 813 683 XbaI T'CTAG_A Cuts at: 3547 3547 Size: 6211 XhoI C'TCGA_G Cuts at: 1943 3114 1943 Size: 1171 5040 XmaI C'CCGG_G Cuts at: 1870 3536 1870 Size: 1666 4545 XmnI GAAnn'nnTTC Cuts at: 2699 4340 2699 Size: 1641 4570 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4019 276 Size: 53 83 186 936 2485 2468 Fragments arranged by size: 2485 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI