(Circular) (Six-base) MAPSORT of: pFN21AB5836.seq Check: 6972 from: 1 to: 6148 pFN21AB5836 6148 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2024 3958 278 Size: 53 83 186 936 488 1934 2468 Fragments arranged by size: 2468 1934 936 488 186 83 53 AccI GT'mk_AC Cuts at: 1937 3491 1937 Size: 1554 4594 Acc65I G'GTAC_C Cuts at: 3469 3469 Size: 6148 AclI AA'CG_TT Cuts at: 1563 4275 4648 1563 Size: 2712 373 3063 Fragments arranged by size: 3063 2712 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2370 4211 5595 5863 1632 Size: 738 1841 1384 268 1917 Fragments arranged by size: 1917 1841 1384 738 268 AflII C'TTAA_G Cuts at: 820 1017 2296 820 Size: 197 1279 4672 Fragments arranged by size: 4672 1279 197 AflIII A'CryG_T Cuts at: 1203 5047 6024 1203 Size: 3844 977 1327 Fragments arranged by size: 3844 1327 977 AgeI A'CCGG_T Cuts at: 4979 4979 Size: 6148 AhdI GACnn_n'nnGTC Cuts at: 4877 4877 Size: 6148 AleI CACnn'nnGTG Cuts at: 1166 3021 1166 Size: 1855 4293 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6148 AlwNI CAG_nnn'CTG Cuts at: 3891 5463 3891 Size: 1572 4576 ApaLI G'TGCA_C Cuts at: 4205 5361 4205 Size: 1156 4992 ApoI r'AATT_y Cuts at: 1493 2404 2530 2698 2925 3083 3457 3683 3835 Size: 911 126 168 227 158 374 226 152 Cuts at: 3835 5944 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 911 374 227 226 168 158 152 126 AseI AT'TA_AT Cuts at: 160 1032 2286 4702 160 Size: 872 1254 2416 1606 Fragments arranged by size: 2416 1606 1254 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6148 AvaI C'yCGr_G Cuts at: 1870 1943 3473 1870 Size: 73 1530 4545 Fragments arranged by size: 4545 1530 73 BamHI G'GATC_C Cuts at: 3478 3845 3478 Size: 367 5781 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3469 4924 618 Size: 325 217 627 1682 1455 1842 Fragments arranged by size: 1842 1682 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3064 3467 1391 Size: 1673 403 4072 Fragments arranged by size: 4072 1673 403 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5177 BbvCI CC'TCA_GC Cuts at: 2049 2049 Size: 6148 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4339 4373 1903 Size: 34 2402 34 3678 Fragments arranged by size: 3678 2402 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3209 4040 5250 3209 Size: 831 1210 4107 Fragments arranged by size: 4107 1210 831 BclI T'GATC_A Cuts at: 1548 1548 Size: 6148 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4759 136 Size: 107 122 71 631 605 3087 1525 Fragments arranged by size: 3087 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2821 6143 2821 Size: 3322 2826 BlpI GC'TnA_GC Cuts at: 3565 3565 Size: 6148 Bme1580I G_kGCm'C Cuts at: 1790 4209 5365 1790 Size: 2419 1156 2573 Fragments arranged by size: 2573 2419 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6148 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3879 4837 442 Size: 416 53 83 399 24 2462 958 1753 Fragments arranged by size: 2462 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6148 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2160 4808 1239 Size: 921 2648 2579 Fragments arranged by size: 2648 2579 921 Bpu10I CC'TnA_GC Cuts at: 2049 2049 Size: 6148 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2230 4273 5138 5436 2230 Size: 2043 865 298 2942 Fragments arranged by size: 2942 2043 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4811 882 Size: 617 3312 2219 Fragments arranged by size: 3312 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3939 493 Size: 711 2735 2702 Fragments arranged by size: 2735 2702 711 BsaBI GATnn'nnATC Cuts at: 6142 6142 Size: 6148 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2021 3955 4337 4972 Size: 53 83 186 936 488 1934 382 635 Cuts at: 4972 275 Size: 1451 Fragments arranged by size: 1934 1451 936 635 488 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4581 4979 5253 5400 5811 4581 Size: 398 274 147 411 4918 Fragments arranged by size: 4918 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5923 Fragments arranged by size: 5923 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6148 BseYI C'CCAG_C Cuts at: 5351 5847 5351 Size: 496 5652 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5575 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3598 4359 5387 1540 Size: 275 186 1597 761 1028 2301 Fragments arranged by size: 2301 1597 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4209 4294 5365 4209 Size: 85 1071 4992 Fragments arranged by size: 4992 1071 85 BsmI GAATG_Cn' Cuts at: 3659 3752 3659 Size: 93 6055 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3064 3467 4209 4294 5365 1391 Size: 399 1274 403 742 85 1071 2174 Fragments arranged by size: 2174 1274 1071 742 403 399 85 BspEI T'CCGG_A Cuts at: 5811 5811 Size: 6148 BspHI T'CATG_A Cuts at: 4037 5767 4037 Size: 1730 4418 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3503 844 Size: 2659 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2576 4035 1394 Size: 429 753 1459 3507 Fragments arranged by size: 3507 1459 753 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4643 4817 65 Size: 1183 3395 174 1396 Fragments arranged by size: 3395 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4792 4979 1700 Size: 110 2982 187 2869 Fragments arranged by size: 2982 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6148 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6148 BssSI C'ACGA_G Cuts at: 4208 5220 4208 Size: 1012 5136 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6148 BstBI TT'CG_AA Cuts at: 4952 4952 Size: 6148 BstEII G'GTnAC_C Cuts at: 1184 2656 1184 Size: 1472 4676 BstXI CCAn_nnnn'nTGG Cuts at: 2973 2973 Size: 6148 BstYI r'GATC_y Cuts at: 1972 2188 2821 3478 3845 4230 4247 5699 5785 Size: 216 633 657 367 385 17 1452 86 Cuts at: 5785 5797 6143 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 657 633 385 367 346 216 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2145 513 Size: 552 939 141 4516 Fragments arranged by size: 4516 939 552 141 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5409 Fragments arranged by size: 5409 683 56 BtsI GCAGTG_nn' Cuts at: 3084 3664 4458 4478 3084 Size: 580 794 20 4754 Fragments arranged by size: 4754 794 580 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6148 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6113 DraI TTT'AAA Cuts at: 2535 3452 2535 Size: 917 5231 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5155 809 Size: 507 3839 1802 Fragments arranged by size: 3839 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2106 2937 3595 Size: 54 1425 50 275 18 276 831 658 Cuts at: 3595 4484 6113 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 831 658 276 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3595 1537 Size: 275 1783 4090 Fragments arranged by size: 4090 1783 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2346 3192 4078 1353 Size: 993 846 886 3423 Fragments arranged by size: 3423 993 886 846 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4735 5109 5255 387 Size: 1064 138 3146 374 146 1280 Fragments arranged by size: 3146 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2160 2370 4211 4808 5595 5863 1239 Size: 393 528 210 1841 597 787 268 1524 Fragments arranged by size: 1841 1524 787 597 528 393 268 210 EcoNI CCTnn'n_nnAGG Cuts at: 2784 2784 Size: 6148 EcoRI G'AATT_C Cuts at: 2404 3083 3457 3835 2404 Size: 679 374 378 4717 Fragments arranged by size: 4717 679 378 374 FspI TGC'GCA Cuts at: 4654 4654 Size: 6148 HaeII r_GCGC'y Cuts at: 1678 3240 5295 1678 Size: 1562 2055 2531 Fragments arranged by size: 2531 2055 1562 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3207 3239 4789 4821 4863 Size: 32 135 32 1635 32 1550 32 42 Cuts at: 4863 4895 1373 Size: 32 2626 Fragments arranged by size: 2626 1635 1550 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3405 3492 3738 669 Size: 988 281 1467 87 246 3079 Fragments arranged by size: 3079 1467 988 281 246 87 HindIII A'AGCT_T Cuts at: 748 748 Size: 6148 HpaI GTT'AAC Cuts at: 3405 3738 3405 Size: 333 5815 KpnI G_GTAC'C Cuts at: 3473 3473 Size: 6148 MfeI C'AATT_G Cuts at: 3747 3747 Size: 6148 MluI A'CGCG_T Cuts at: 6024 6024 Size: 6148 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2340 5262 5446 1305 Size: 1035 2922 184 2007 Fragments arranged by size: 2922 2007 1035 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2939 10 Size: 54 1425 343 1107 3219 Fragments arranged by size: 3219 1425 1107 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2645 2805 2833 3021 3207 4106 4465 Size: 648 1479 160 28 188 186 899 359 Cuts at: 4465 4624 518 Size: 159 2042 Fragments arranged by size: 2042 1479 899 648 359 188 186 160 159 28 MspA1I CmG'CkG Cuts at: 1606 2048 3564 4241 5389 5634 1606 Size: 442 1516 677 1148 245 2120 Fragments arranged by size: 2120 1516 1148 677 442 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2145 513 Size: 552 939 141 4516 Fragments arranged by size: 4516 939 552 141 NdeI CA'TA_TG Cuts at: 387 387 Size: 6148 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6148 NotI GC'GGCC_GC Cuts at: 3595 3595 Size: 6148 NspI r_CATG'y Cuts at: 1132 1229 3506 5051 1132 Size: 97 2277 1545 2229 Fragments arranged by size: 2277 2229 1545 97 PacI TTA_AT'TAA Cuts at: 2373 2373 Size: 6148 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6148 PciI A'CATG_T Cuts at: 5047 5047 Size: 6148 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6148 PmeI GTTT'AAAC Cuts at: 3452 3452 Size: 6148 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4217 4249 5730 5762 4217 Size: 32 1481 32 4603 Fragments arranged by size: 4603 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3315 1613 Size: 1702 4446 PsiI TTA'TAA Cuts at: 3718 3718 Size: 6148 PstI C_TGCA'G Cuts at: 830 2096 3500 830 Size: 1266 1404 3478 Fragments arranged by size: 3478 1404 1266 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6148 PvuII CAG'CTG Cuts at: 2048 2048 Size: 6148 SalI G'TCGA_C Cuts at: 1936 3490 1936 Size: 1554 4594 SapI GCTCTTCn'nnn_ Cuts at: 2346 3192 2346 Size: 846 5302 SbfI CC_TGCA'GG Cuts at: 3500 3500 Size: 6148 ScaI AGT'ACT Cuts at: 3365 4396 6108 3365 Size: 1031 1712 3405 Fragments arranged by size: 3405 1712 1031 SfcI C'TryA_G Cuts at: 826 1045 2092 3496 4631 5312 5503 826 Size: 219 1047 1404 1135 681 191 1471 Fragments arranged by size: 1471 1404 1135 1047 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6148 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6148 SmaI CCC'GGG Cuts at: 1872 3475 1872 Size: 1603 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2245 2296 4252 5153 5415 820 Size: 197 926 302 51 1956 901 262 1553 Fragments arranged by size: 1956 1553 926 901 302 262 197 51 SnaBI TAC'GTA Cuts at: 493 3939 493 Size: 3446 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6148 SphI G_CATG'C Cuts at: 1132 3506 1132 Size: 2374 3774 SspI AAT'ATT Cuts at: 5 52 3053 4072 5 Size: 47 3001 1019 2081 Fragments arranged by size: 3001 2081 1019 47 StuI AGG'CCT Cuts at: 1517 3069 1517 Size: 1552 4596 StyI C'CwwG_G Cuts at: 513 1065 2004 2145 2940 3070 3313 3587 513 Size: 552 939 141 795 130 243 274 3074 Fragments arranged by size: 3074 939 795 552 274 243 141 130 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4175 4192 4345 4530 4975 325 Size: 1255 2595 17 153 185 445 1498 Fragments arranged by size: 2595 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3363 4394 6106 Size: 274 80 33 51 1443 1386 1031 1712 Cuts at: 6106 96 Size: 138 Fragments arranged by size: 1712 1443 1386 1031 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6108 6140 6108 Size: 32 6116 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6148 XbaI T'CTAG_A Cuts at: 3484 3484 Size: 6148 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6148 XmaI C'CCGG_G Cuts at: 1870 3473 1870 Size: 1603 4545 XmnI GAAnn'nnTTC Cuts at: 2087 2631 2715 3344 4277 2087 Size: 544 84 629 933 3958 Fragments arranged by size: 3958 933 629 544 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2022 3956 276 Size: 53 83 186 936 488 1934 2468 Fragments arranged by size: 2468 1934 936 488 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PacI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI