Restriction map for
pFN21AB5824
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AviII AvrII BalI
BanII BbeI BbrPI BbvCI BcgI
BcnI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmI Bsp1407I BspEI BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HindIII HpaI KasI KpnI KspI
MaeII MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NciI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PstI PvuI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI StuI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 136 GTAGAC GT^[AC][GT]AC
AflIII 1042 ACATGT A^C[GA][CT]GT
Asp700I 400 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 827 CCCGAG C^[CT]CG[GA]G
BamHI 1090 GGATCC G^GATCC
BbsI 19 GAAGAC GAAGAC(2/6)
BciVI 984 GGATAC GTATCC(6/5)
BclI 1050 TGATCA T^GATCA
BglII 164 AGATCT A^GATCT
BmyI 7 GTGCCC G[AGT]GC[ACT]^C
BpuAI 19 GAAGAC GAAGAC(2/6)
BsaBI 165 GATCTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsoBI 827 CCCGAG C^[CT]CG[GA]G
Bsp1286I 7 GTGCCC G[AGT]GC[ACT]^C
BspDI 700 ATCGAT AT^CGAT
BspHI 189 TCATGA T^CATGA
BspLU11I 1042 ACATGT A^CATGT
BsrDI 598 GCAATG GCAATG(2/0)
ClaI 700 ATCGAT AT^CGAT
DraII 823 AGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 619 CCATGG C^C[GA][CT]GG
EcoO109I 823 AGGCCCC [GA]G^G[ACGT]CC[CT]
FauI 962 CCCGC CCCGC(4/6)
FbaI 1050 TGATCA T^GATCA
HaeIII 824 GGCC GG^CC
HapII 1323 CCGG C^CGG
HincII 1315 GTTGAC GT[CT]^[GA]AC
HindII 1315 GTTGAC GT[CT]^[GA]AC
HpaII 1323 CCGG C^CGG
HphI 1336 GGTGA GGTGA(8/7)
MamI 165 GATCTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 1323 CCGG C^CGG
NcoI 619 CCATGG C^CATGG
PshAI 637 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 639 CAGCTG CAG^CTG
RcaI 189 TCATGA T^CATGA
SfcI 258 CTGTAG C^T[GA][CT]AG
SmlI 805 CTCAAG C^T[CT][GA]AG
SphI 302 GCATGC GCATG^C
TaqI 701 TCGA T^CGA
TthHB8I 701 TCGA T^CGA
XcmI 562 CCAGTTGATGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 400 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AciI 963 CCGC CCGC(-3/-1)
1113 GCGG
AvaII 575 GGTCC G^G[AT]CC
624 GGACC
BanI 52 GGTGCC G^G[CT][GA]CC
1081 GGCACC
BfaI 715 CTAG C^TAG
920 CTAG
BseRI 710 CTCCTC GAGGAG(10/8)
266 GAGGAG
BsmFI 26 GGGAC GGGAC(10/14)
623 GGGAC
BstYI 164 AGATCT [GA]^GATC[CT]
1090 GGATCC
EarI 537 GAAGAG CTCTTC(1/4)
542 GAAGAG
EcoT14I 619 CCATGG C^C[AT][AT]GG
688 CCTTGG
Ksp632I 537 GAAGAG CTCTTC(1/4)
542 GAAGAG
MaeI 715 CTAG C^TAG
920 CTAG
MflI 164 AGATCT [GA]^GATC[CT]
1090 GGATCC
MslI 190 CATGAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
297 CATAGGCATG
MspA1I 639 CAGCTG C[AC]G^C[GT]G
1111 CAGCGG
MwoI 598 GCAATGCTCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
709 GCTCCTCTAGC
NdeI 515 CATATG CA^TATG
1054 CATATG
NspI 302 GCATGC [GA]CATG^[CT]
1042 ACATGT
PleI 830 GAGTC GAGTC(4/5)
853 GAGTC
SfaNI 316 GATGC GCATC(5/9)
1153 GATGC
StyI 619 CCATGG C^C[AT][AT]GG
688 CCTTGG
XhoII 164 AGATCT [GA]^GATC[CT]
1090 GGATCC
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbvI BpmI BsaJI BsiYI BslI
BsmAI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HinfI ItaI MaeIII
MboI MboII MnlI MseI MvaI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScaI ScrFI SspI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI