(Circular) (Six-base) MAPSORT of: pFN21AB5824.seq Check: 8402 from: 1 to: 6133 pFN21AB5824 6133 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3943 278 Size: 53 83 186 936 2407 2468 Fragments arranged by size: 2468 2407 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2142 3476 1937 Size: 205 1334 4594 Fragments arranged by size: 4594 1334 205 Acc65I G'GTAC_C Cuts at: 3454 3454 Size: 6133 AclI AA'CG_TT Cuts at: 1563 4260 4633 1563 Size: 2697 373 3063 Fragments arranged by size: 3063 2697 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2118 2561 2826 3392 4196 5580 5848 1632 Size: 486 443 265 566 804 1384 268 1917 Fragments arranged by size: 1917 1384 804 566 486 443 268 265 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5936 AflIII A'CryG_T Cuts at: 1203 3047 5032 6009 1203 Size: 1844 1985 977 1327 Fragments arranged by size: 1985 1844 1327 977 AgeI A'CCGG_T Cuts at: 4964 4964 Size: 6133 AhdI GACnn_n'nnGTC Cuts at: 4862 4862 Size: 6133 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6133 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6133 AlwNI CAG_nnn'CTG Cuts at: 3876 5448 3876 Size: 1572 4561 ApaLI G'TGCA_C Cuts at: 4190 5346 4190 Size: 1156 4977 ApoI r'AATT_y Cuts at: 1493 2210 2552 3289 3442 3668 3820 5929 1493 Size: 717 342 737 153 226 152 2109 1697 Fragments arranged by size: 2109 1697 737 717 342 226 153 152 AseI AT'TA_AT Cuts at: 160 1032 4687 160 Size: 872 3655 1606 Fragments arranged by size: 3655 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6133 AvaI C'yCGr_G Cuts at: 1870 1943 2832 3458 1870 Size: 73 889 626 4545 Fragments arranged by size: 4545 889 626 73 BamHI G'GATC_C Cuts at: 3095 3463 3830 3095 Size: 368 367 5398 Fragments arranged by size: 5398 368 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2057 3086 3454 4909 618 Size: 325 217 627 270 1029 368 1455 1842 Fragments arranged by size: 1842 1455 1029 627 368 325 270 217 BanII G_rGCy'C Cuts at: 1391 3452 1391 Size: 2061 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2031 928 Size: 971 132 5030 Fragments arranged by size: 5030 971 132 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4324 4358 1903 Size: 34 2387 34 3678 Fragments arranged by size: 3678 2387 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2983 4025 5235 2983 Size: 1042 1210 3881 Fragments arranged by size: 3881 1210 1042 BclI T'GATC_A Cuts at: 1548 3055 1548 Size: 1507 4626 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4744 136 Size: 107 122 71 631 605 3072 1525 Fragments arranged by size: 3072 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2169 6128 2169 Size: 3959 2174 BlpI GC'TnA_GC Cuts at: 3550 3550 Size: 6133 Bme1580I G_kGCm'C Cuts at: 1790 2016 4194 5350 1790 Size: 226 2178 1156 2573 Fragments arranged by size: 2573 2178 1156 226 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6133 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2038 2631 2859 Size: 416 53 83 399 24 621 593 228 Cuts at: 2859 3864 4822 442 Size: 1005 958 1753 Fragments arranged by size: 1753 1005 958 621 593 416 399 228 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6133 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2276 2514 2634 2892 2932 4793 1239 Size: 1037 238 120 258 40 1861 2579 Fragments arranged by size: 2579 1861 1037 258 238 120 40 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2795 4258 5123 5421 2795 Size: 1463 865 298 3507 Fragments arranged by size: 3507 1463 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4796 882 Size: 617 3297 2219 Fragments arranged by size: 3297 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3924 493 Size: 711 2720 2702 Fragments arranged by size: 2720 2702 711 BsaBI GATnn'nnATC Cuts at: 2174 6127 2174 Size: 3953 2180 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3940 4322 4957 275 Size: 53 83 186 936 2407 382 635 1451 Fragments arranged by size: 2407 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4566 4964 5238 5385 5796 4566 Size: 398 274 147 411 4903 Fragments arranged by size: 4903 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5908 Fragments arranged by size: 5908 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2286 2706 1186 Size: 1100 420 4613 Fragments arranged by size: 4613 1100 420 BseYI C'CCAG_C Cuts at: 3039 5336 5832 3039 Size: 2297 496 3340 Fragments arranged by size: 3340 2297 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5560 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3583 4344 5372 1540 Size: 275 186 1582 761 1028 2301 Fragments arranged by size: 2301 1582 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4194 4279 5350 4194 Size: 85 1071 4977 Fragments arranged by size: 4977 1071 85 BsmI GAATG_Cn' Cuts at: 3644 3737 3644 Size: 93 6040 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2016 3452 4194 4279 5350 1391 Size: 399 226 1436 742 85 1071 2174 Fragments arranged by size: 2174 1436 1071 742 399 226 85 BspEI T'CCGG_A Cuts at: 5796 5796 Size: 6133 BspHI T'CATG_A Cuts at: 2194 4022 5752 2194 Size: 1828 1730 2575 Fragments arranged by size: 2575 1828 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3488 844 Size: 2644 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4020 1394 Size: 429 2197 3507 Fragments arranged by size: 3507 2197 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2610 4628 4802 65 Size: 1183 1362 2018 174 1396 Fragments arranged by size: 2018 1396 1362 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4777 4964 1700 Size: 110 2967 187 2869 Fragments arranged by size: 2967 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6133 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6133 BssSI C'ACGA_G Cuts at: 4193 5205 4193 Size: 1012 5121 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6133 BstBI TT'CG_AA Cuts at: 4937 4937 Size: 6133 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6133 BstYI r'GATC_y Cuts at: 1972 2169 3095 3463 3830 4215 4232 5684 5770 Size: 197 926 368 367 385 17 1452 86 Cuts at: 5770 5782 6128 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 926 385 368 367 346 197 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2624 513 Size: 552 939 620 4022 Fragments arranged by size: 4022 939 620 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5394 Fragments arranged by size: 5394 683 56 BtsI GCAGTG_nn' Cuts at: 2016 2118 3649 4443 4463 2016 Size: 102 1531 794 20 3686 Fragments arranged by size: 3686 1531 794 102 20 ClaI AT'CG_AT Cuts at: 1554 2706 1554 Size: 1152 4981 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2488 2523 529 Size: 35 1924 35 4139 Fragments arranged by size: 4139 1924 35 35 DraI TTT'AAA Cuts at: 3437 3437 Size: 6133 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5140 809 Size: 507 3824 1802 Fragments arranged by size: 3824 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3580 4469 6098 Size: 54 1425 50 275 18 1750 889 1629 Cuts at: 6098 8 Size: 43 Fragments arranged by size: 1750 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3580 1537 Size: 275 1768 4090 Fragments arranged by size: 4090 1768 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2537 2542 4063 1353 Size: 1184 5 1521 3423 Fragments arranged by size: 3423 1521 1184 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4720 5094 5240 387 Size: 1064 138 3131 374 146 1280 Fragments arranged by size: 3131 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2118 2276 2514 2561 2634 2826 2892 Size: 393 486 158 238 47 73 192 66 Cuts at: 2892 2932 3392 4196 4793 5580 5848 1239 Size: 40 460 804 597 787 268 1524 Fragments arranged by size: 1524 804 787 597 486 460 393 268 238 192 158 73 66 47 40 EcoO109I rG'GnC_Cy Cuts at: 2829 2829 Size: 6133 EcoRI G'AATT_C Cuts at: 3442 3820 3442 Size: 378 5755 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2093 3382 2093 Size: 1289 4844 FspI TGC'GCA Cuts at: 4639 4639 Size: 6133 HaeII r_GCGC'y Cuts at: 1678 5280 1678 Size: 3602 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2564 2596 2738 2770 4774 Size: 32 135 32 992 32 142 32 2004 Cuts at: 4774 4806 4848 4880 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2004 992 142 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3322 3477 3723 669 Size: 988 281 1384 155 246 3079 Fragments arranged by size: 3079 1384 988 281 246 155 HindIII A'AGCT_T Cuts at: 748 748 Size: 6133 HpaI GTT'AAC Cuts at: 3723 3723 Size: 6133 KpnI G_GTAC'C Cuts at: 3458 3458 Size: 6133 MfeI C'AATT_G Cuts at: 3732 3732 Size: 6133 MluI A'CGCG_T Cuts at: 6009 6009 Size: 6133 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2397 5247 5431 1305 Size: 1092 2850 184 2007 Fragments arranged by size: 2850 2007 1092 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4311 Fragments arranged by size: 4311 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2199 2306 4091 4450 4609 518 Size: 648 843 190 107 1785 359 159 2042 Fragments arranged by size: 2042 1785 843 648 359 190 159 107 MspA1I CmG'CkG Cuts at: 1606 2646 3118 3549 4226 5374 5619 1606 Size: 1040 472 431 677 1148 245 2120 Fragments arranged by size: 2120 1148 1040 677 472 431 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2624 513 Size: 552 939 620 4022 Fragments arranged by size: 4022 939 620 552 NdeI CA'TA_TG Cuts at: 387 2521 3060 387 Size: 2134 539 3460 Fragments arranged by size: 3460 2134 539 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6133 NotI GC'GGCC_GC Cuts at: 3580 3580 Size: 6133 NspI r_CATG'y Cuts at: 1132 1229 2311 3051 3491 5036 1132 Size: 97 1082 740 440 1545 2229 Fragments arranged by size: 2229 1545 1082 740 440 97 PasI CC'CwG_GG Cuts at: 1348 2047 1348 Size: 699 5434 PciI A'CATG_T Cuts at: 3047 5032 3047 Size: 1985 4148 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6133 PmeI GTTT'AAAC Cuts at: 3437 3437 Size: 6133 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4202 4234 5715 5747 4202 Size: 32 1481 32 4588 Fragments arranged by size: 4588 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2646 1613 Size: 1033 5100 PsiI TTA'TAA Cuts at: 3703 3703 Size: 6133 PstI C_TGCA'G Cuts at: 830 3485 830 Size: 2655 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6133 PvuII CAG'CTG Cuts at: 2646 2646 Size: 6133 SalI G'TCGA_C Cuts at: 1936 3475 1936 Size: 1539 4594 SbfI CC_TGCA'GG Cuts at: 3485 3485 Size: 6133 ScaI AGT'ACT Cuts at: 2261 2595 2945 4381 6093 2261 Size: 334 350 1436 1712 2301 Fragments arranged by size: 2301 1712 1436 350 334 SfcI C'TryA_G Cuts at: 826 1045 2263 3481 4616 5297 5488 826 Size: 219 1218 1218 1135 681 191 1471 Fragments arranged by size: 1471 1218 1218 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6133 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6133 SmaI CCC'GGG Cuts at: 1872 3460 1872 Size: 1588 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2810 4237 5138 5400 820 Size: 197 926 867 1427 901 262 1553 Fragments arranged by size: 1553 1427 926 901 867 262 197 SnaBI TAC'GTA Cuts at: 493 3924 493 Size: 3431 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6133 SphI G_CATG'C Cuts at: 1132 2311 3491 1132 Size: 1179 1180 3774 Fragments arranged by size: 3774 1180 1179 SspI AAT'ATT Cuts at: 5 52 2158 2246 3315 4057 5 Size: 47 2106 88 1069 742 2081 Fragments arranged by size: 2106 2081 1069 742 88 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6133 StyI C'CwwG_G Cuts at: 513 1065 2004 2624 2693 3572 513 Size: 552 939 620 69 879 3074 Fragments arranged by size: 3074 939 879 620 552 69 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4160 4177 4330 4515 4960 325 Size: 1255 2580 17 153 185 445 1498 Fragments arranged by size: 2580 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2259 2593 2943 Size: 274 80 33 51 1443 282 334 350 Cuts at: 2943 4379 6091 96 Size: 1436 1712 138 Fragments arranged by size: 1712 1443 1436 350 334 282 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2873 2905 6093 6125 2873 Size: 32 3188 32 2881 Fragments arranged by size: 3188 2881 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6133 XbaI T'CTAG_A Cuts at: 3469 3469 Size: 6133 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2574 2574 Size: 6133 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6133 XmaI C'CCGG_G Cuts at: 1870 3458 1870 Size: 1588 4545 XmnI GAAnn'nnTTC Cuts at: 2409 4262 2409 Size: 1853 4280 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3941 276 Size: 53 83 186 936 2407 2468 Fragments arranged by size: 2468 2407 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI