(Circular) (Six-base) MAPSORT of: pFN21AB7972.seq Check: 3283 from: 1 to: 5938 pFN21AB7972 5938 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3748 278 Size: 53 83 186 936 2212 2468 Fragments arranged by size: 2468 2212 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3083 3281 1937 Size: 1146 198 4594 Fragments arranged by size: 4594 1146 198 Acc65I G'GTAC_C Cuts at: 3259 3259 Size: 5938 AclI AA'CG_TT Cuts at: 1563 4065 4438 1563 Size: 2502 373 3063 Fragments arranged by size: 3063 2502 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2031 4001 5385 5653 1632 Size: 399 1970 1384 268 1917 Fragments arranged by size: 1970 1917 1384 399 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5741 AflIII A'CryG_T Cuts at: 1203 2033 2959 4837 5814 1203 Size: 830 926 1878 977 1327 Fragments arranged by size: 1878 1327 977 926 830 AgeI A'CCGG_T Cuts at: 4769 4769 Size: 5938 AhdI GACnn_n'nnGTC Cuts at: 4667 4667 Size: 5938 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5938 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5938 AlwNI CAG_nnn'CTG Cuts at: 3681 5253 3681 Size: 1572 4366 ApaLI G'TGCA_C Cuts at: 3995 5151 3995 Size: 1156 4782 ApoI r'AATT_y Cuts at: 1493 2512 2693 3197 3247 3473 3625 5734 1493 Size: 1019 181 504 50 226 152 2109 1697 Fragments arranged by size: 2109 1697 1019 504 226 181 152 50 AseI AT'TA_AT Cuts at: 160 1032 4492 160 Size: 872 3460 1606 Fragments arranged by size: 3460 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5938 AvaI C'yCGr_G Cuts at: 1870 1943 2108 3263 1870 Size: 73 165 1155 4545 Fragments arranged by size: 4545 1155 165 73 BamHI G'GATC_C Cuts at: 2353 3268 3635 2353 Size: 915 367 4656 Fragments arranged by size: 4656 915 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2138 3259 4714 618 Size: 325 217 627 351 1121 1455 1842 Fragments arranged by size: 1842 1455 1121 627 351 325 217 BanII G_rGCy'C Cuts at: 1391 3257 1391 Size: 1866 4072 BbeI G_GCGC'C Cuts at: 2142 2142 Size: 5938 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4967 BbvCI CC'TCA_GC Cuts at: 2192 2303 2192 Size: 111 5827 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4129 4163 1903 Size: 34 2192 34 3678 Fragments arranged by size: 3678 2192 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3033 3830 5040 3033 Size: 797 1210 3931 Fragments arranged by size: 3931 1210 797 BclI T'GATC_A Cuts at: 1548 1548 Size: 5938 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2025 2261 4549 Size: 107 122 71 631 605 353 236 2288 Cuts at: 4549 136 Size: 1525 Fragments arranged by size: 2288 1525 631 605 353 236 122 107 71 BglII A'GATC_T Cuts at: 5933 5933 Size: 5938 BlpI GC'TnA_GC Cuts at: 2298 3355 2298 Size: 1057 4881 Bme1580I G_kGCm'C Cuts at: 1790 3999 5155 1790 Size: 2209 1156 2573 Fragments arranged by size: 2573 2209 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5938 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2057 2927 3200 Size: 416 53 83 399 24 640 870 273 Cuts at: 3200 3669 4627 442 Size: 469 958 1753 Fragments arranged by size: 1753 958 870 640 469 416 399 273 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5938 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2306 2663 4598 1239 Size: 1067 357 1935 2579 Fragments arranged by size: 2579 1935 1067 357 Bpu10I CC'TnA_GC Cuts at: 2192 2303 2721 2192 Size: 111 418 5409 Fragments arranged by size: 5409 418 111 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2360 4063 4928 5226 2360 Size: 1703 865 298 3072 Fragments arranged by size: 3072 1703 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4601 882 Size: 617 3102 2219 Fragments arranged by size: 3102 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2034 3729 493 Size: 711 830 1695 2702 Fragments arranged by size: 2702 1695 830 711 BsaBI GATnn'nnATC Cuts at: 5932 5932 Size: 5938 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2139 3745 4127 4762 Size: 53 83 186 936 606 1606 382 635 Cuts at: 4762 275 Size: 1451 Fragments arranged by size: 1606 1451 936 635 606 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2368 4371 4769 5043 5190 5601 2368 Size: 2003 398 274 147 411 2705 Fragments arranged by size: 2705 2003 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2051 2081 1178 Size: 30 165 30 648 30 5035 Fragments arranged by size: 5035 648 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5938 BseYI C'CCAG_C Cuts at: 5141 5637 5141 Size: 496 5442 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3149 1163 Size: 573 1413 3952 Fragments arranged by size: 3952 1413 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2245 3388 4149 5177 1540 Size: 275 186 244 1143 761 1028 2301 Fragments arranged by size: 2301 1143 1028 761 275 244 186 BsiHKAI G_wGCw'C Cuts at: 2727 3999 4084 5155 2727 Size: 1272 85 1071 3510 Fragments arranged by size: 3510 1272 1071 85 BsmI GAATG_Cn' Cuts at: 2807 3165 3449 3542 2807 Size: 358 284 93 5203 Fragments arranged by size: 5203 358 284 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2727 3257 3999 4084 5155 1391 Size: 399 937 530 742 85 1071 2174 Fragments arranged by size: 2174 1071 937 742 530 399 85 BspEI T'CCGG_A Cuts at: 2368 5601 2368 Size: 3233 2705 BspHI T'CATG_A Cuts at: 2995 3827 5557 2995 Size: 832 1730 3376 Fragments arranged by size: 3376 1730 832 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3293 844 Size: 2449 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2213 3825 1394 Size: 429 390 1612 3507 Fragments arranged by size: 3507 1612 429 390 BsrDI GCAATG_nn' Cuts at: 65 1248 4433 4607 65 Size: 1183 3185 174 1396 Fragments arranged by size: 3185 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2203 4582 4769 1700 Size: 110 393 2379 187 2869 Fragments arranged by size: 2869 2379 393 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5938 BssHII G'CGCG_C Cuts at: 1925 2122 1925 Size: 197 5741 BssSI C'ACGA_G Cuts at: 3998 5010 3998 Size: 1012 4926 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5938 BstBI TT'CG_AA Cuts at: 4742 4742 Size: 5938 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5938 BstXI CCAn_nnnn'nTGG Cuts at: 2272 2687 2272 Size: 415 5523 BstYI r'GATC_y Cuts at: 1972 2269 2353 3268 3635 4020 4037 5489 5575 Size: 297 84 915 367 385 17 1452 86 Cuts at: 5575 5587 5933 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 915 385 367 346 297 86 84 17 12 BstZ17I GTA'TAC Cuts at: 3084 3084 Size: 5938 BtgI C'CryG_G Cuts at: 513 1065 2004 2256 513 Size: 552 939 252 4195 Fragments arranged by size: 4195 939 552 252 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5199 Fragments arranged by size: 5199 683 56 BtsI GCAGTG_nn' Cuts at: 2080 3454 4248 4268 2080 Size: 1374 794 20 3750 Fragments arranged by size: 3750 1374 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5938 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5903 DraI TTT'AAA Cuts at: 3242 3242 Size: 5938 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4945 809 Size: 507 3629 1802 Fragments arranged by size: 3629 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2262 3385 4274 Size: 54 1425 50 275 18 432 1123 889 Cuts at: 4274 5903 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1123 889 432 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3385 1537 Size: 275 1573 4090 Fragments arranged by size: 4090 1573 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3868 1353 Size: 2515 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4525 4899 5045 387 Size: 1064 138 2936 374 146 1280 Fragments arranged by size: 2936 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2031 2306 2663 4001 4598 5385 5653 Size: 393 399 275 357 1338 597 787 268 Cuts at: 5653 1239 Size: 1524 Fragments arranged by size: 1524 1338 787 597 399 393 357 275 268 EcoO109I rG'GnC_Cy Cuts at: 2059 3127 2059 Size: 1068 4870 EcoRI G'AATT_C Cuts at: 2693 3197 3247 3625 2693 Size: 504 50 378 5006 Fragments arranged by size: 5006 504 378 50 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2558 2558 Size: 5938 FspI TGC'GCA Cuts at: 4444 4444 Size: 5938 HaeII r_GCGC'y Cuts at: 1678 2142 5085 1678 Size: 464 2943 2531 Fragments arranged by size: 2943 2531 464 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2051 2083 2468 2500 3007 Size: 32 135 32 479 32 385 32 507 Cuts at: 3007 3039 4579 4611 4653 4685 1373 Size: 32 1540 32 42 32 2626 Fragments arranged by size: 2626 1540 507 479 385 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3282 3528 669 Size: 988 281 1344 246 3079 Fragments arranged by size: 3079 1344 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5938 HpaI GTT'AAC Cuts at: 3528 3528 Size: 5938 KasI G'GCGC_C Cuts at: 2138 2138 Size: 5938 KpnI G_GTAC'C Cuts at: 3263 3263 Size: 5938 MfeI C'AATT_G Cuts at: 3537 3537 Size: 5938 MluI A'CGCG_T Cuts at: 5814 5814 Size: 5938 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2265 5052 5236 1305 Size: 960 2787 184 2007 Fragments arranged by size: 2787 2007 960 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2264 10 Size: 54 1425 343 432 3684 Fragments arranged by size: 3684 1425 432 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2997 3896 4255 4414 518 Size: 648 1831 899 359 159 2042 Fragments arranged by size: 2042 1831 899 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2234 2258 3354 4031 5179 5424 1606 Size: 628 24 1096 677 1148 245 2120 Fragments arranged by size: 2120 1148 1096 677 628 245 24 NaeI GCC'GGC Cuts at: 2205 2205 Size: 5938 NarI GG'CG_CC Cuts at: 2139 2139 Size: 5938 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4447 Fragments arranged by size: 4447 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5938 NgoMIV G'CCGG_C Cuts at: 2203 2203 Size: 5938 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5938 NotI GC'GGCC_GC Cuts at: 3385 3385 Size: 5938 NspI r_CATG'y Cuts at: 1132 1229 2492 2963 3296 4841 1132 Size: 97 1263 471 333 1545 2229 Fragments arranged by size: 2229 1545 1263 471 333 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5938 PciI A'CATG_T Cuts at: 2959 4837 2959 Size: 1878 4060 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5938 PfoI T'CCnGG_A Cuts at: 3223 3223 Size: 5938 PmeI GTTT'AAAC Cuts at: 3242 3242 Size: 5938 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4007 4039 5520 5552 4007 Size: 32 1481 32 4393 Fragments arranged by size: 4393 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2059 3127 2059 Size: 1068 4870 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5938 PsiI TTA'TAA Cuts at: 2781 3508 2781 Size: 727 5211 PstI C_TGCA'G Cuts at: 830 2282 2456 3290 830 Size: 1452 174 834 3478 Fragments arranged by size: 3478 1452 834 174 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5938 PvuII CAG'CTG Cuts at: 2234 2234 Size: 5938 SacII CC_GC'GG Cuts at: 2259 2259 Size: 5938 SalI G'TCGA_C Cuts at: 1936 3280 1936 Size: 1344 4594 SbfI CC_TGCA'GG Cuts at: 3290 3290 Size: 5938 ScaI AGT'ACT Cuts at: 4186 5898 4186 Size: 1712 4226 SexAI A'CCwGG_T Cuts at: 2382 2382 Size: 5938 SfcI C'TryA_G Cuts at: 826 1045 2278 2452 3286 4421 5102 5293 826 Size: 219 1233 174 834 1135 681 191 1471 Fragments arranged by size: 1471 1233 1135 834 681 219 191 174 SfoI GGC'GCC Cuts at: 2140 2140 Size: 5938 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5938 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5938 SmaI CCC'GGG Cuts at: 1872 3265 1872 Size: 1393 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2339 4042 4943 5205 820 Size: 197 926 396 1703 901 262 1553 Fragments arranged by size: 1703 1553 926 901 396 262 197 SnaBI TAC'GTA Cuts at: 493 3729 493 Size: 3236 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5938 SphI G_CATG'C Cuts at: 1132 3296 1132 Size: 2164 3774 SspI AAT'ATT Cuts at: 5 52 3862 5 Size: 47 3810 2081 Fragments arranged by size: 3810 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5938 StyI C'CwwG_G Cuts at: 513 1065 2004 3377 513 Size: 552 939 1373 3074 Fragments arranged by size: 3074 1373 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2036 3085 3965 3982 4135 4320 4765 Size: 1255 456 1049 880 17 153 185 445 Cuts at: 4765 325 Size: 1498 Fragments arranged by size: 1498 1255 1049 880 456 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4184 5896 96 Size: 274 80 33 51 1443 2207 1712 138 Fragments arranged by size: 2207 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5898 5930 5898 Size: 32 5906 Tth111I GACn'n_nGTC Cuts at: 1316 2063 1316 Size: 747 5191 XbaI T'CTAG_A Cuts at: 3274 3274 Size: 5938 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5938 XmaI C'CCGG_G Cuts at: 1870 3263 1870 Size: 1393 4545 XmnI GAAnn'nnTTC Cuts at: 2364 4067 2364 Size: 1703 4235 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3746 276 Size: 53 83 186 936 2212 2468 Fragments arranged by size: 2468 2212 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SanDI SapI SfiI SrfI SwaI XcmI