(Circular) (Six-base) MAPSORT of: pFN21AB7968.seq Check: 6068 from: 1 to: 5926 pFN21AB7968 5926 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3736 278 Size: 53 83 186 936 2200 2468 Fragments arranged by size: 2468 2200 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3269 1937 Size: 1332 4594 Acc65I G'GTAC_C Cuts at: 3247 3247 Size: 5926 AclI AA'CG_TT Cuts at: 1563 4053 4426 1563 Size: 2490 373 3063 Fragments arranged by size: 3063 2490 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2064 2134 3047 3131 3989 5373 5641 1632 Size: 432 70 913 84 858 1384 268 1917 Fragments arranged by size: 1917 1384 913 858 432 268 84 70 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5729 AflIII A'CryG_T Cuts at: 1203 3066 4825 5802 1203 Size: 1863 1759 977 1327 Fragments arranged by size: 1863 1759 1327 977 AgeI A'CCGG_T Cuts at: 4757 4757 Size: 5926 AhdI GACnn_n'nnGTC Cuts at: 4655 4655 Size: 5926 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5926 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2951 1184 Size: 1767 4159 AlwNI CAG_nnn'CTG Cuts at: 2129 2287 2476 3669 5241 2129 Size: 158 189 1193 1572 2814 Fragments arranged by size: 2814 1572 1193 189 158 ApaI G_GGCC'C Cuts at: 2102 2102 Size: 5926 ApaLI G'TGCA_C Cuts at: 3983 5139 3983 Size: 1156 4770 ApoI r'AATT_y Cuts at: 1493 3016 3235 3461 3613 5722 1493 Size: 1523 219 226 152 2109 1697 Fragments arranged by size: 2109 1697 1523 226 219 152 AseI AT'TA_AT Cuts at: 160 1032 4480 160 Size: 872 3448 1606 Fragments arranged by size: 3448 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5926 AvaI C'yCGr_G Cuts at: 1870 1943 2234 2316 3251 1870 Size: 73 291 82 935 4545 Fragments arranged by size: 4545 935 291 82 73 AvrII C'CTAG_G Cuts at: 2501 2501 Size: 5926 BamHI G'GATC_C Cuts at: 3126 3256 3623 3126 Size: 130 367 5429 Fragments arranged by size: 5429 367 130 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3247 4702 618 Size: 325 217 627 1460 1455 1842 Fragments arranged by size: 1842 1460 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2102 2136 2525 2570 3188 3245 1391 Size: 711 34 389 45 618 57 4072 Fragments arranged by size: 4072 711 618 389 57 45 34 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4955 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2196 2230 4117 4151 1903 Size: 34 259 34 1887 34 3678 Fragments arranged by size: 3678 1887 259 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3818 5028 3818 Size: 1210 4716 BclI T'GATC_A Cuts at: 1548 1548 Size: 5926 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4537 136 Size: 107 122 71 631 605 2865 1525 Fragments arranged by size: 2865 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2509 5921 2509 Size: 3412 2514 BlpI GC'TnA_GC Cuts at: 2564 2965 3343 2564 Size: 401 378 5147 Fragments arranged by size: 5147 401 378 Bme1580I G_kGCm'C Cuts at: 1790 2102 3987 5143 1790 Size: 312 1885 1156 2573 Fragments arranged by size: 2573 1885 1156 312 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5926 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3657 4615 442 Size: 416 53 83 399 24 2240 958 1753 Fragments arranged by size: 2240 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5926 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2212 2790 2212 Size: 578 5348 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2511 3156 4586 1239 Size: 1272 645 1430 2579 Fragments arranged by size: 2579 1430 1272 645 Bpu10I CC'TnA_GC Cuts at: 2130 2130 Size: 5926 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4051 4916 5214 4051 Size: 865 298 4763 Fragments arranged by size: 4763 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3052 4589 882 Size: 617 1553 1537 2219 Fragments arranged by size: 2219 1553 1537 617 BsaAI yAC'GTr Cuts at: 493 1204 3012 3069 3717 493 Size: 711 1808 57 648 2702 Fragments arranged by size: 2702 1808 711 648 57 BsaBI GATnn'nnATC Cuts at: 5920 5920 Size: 5926 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3733 4115 4750 275 Size: 53 83 186 936 2200 382 635 1451 Fragments arranged by size: 2200 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4359 4757 5031 5178 5589 4359 Size: 398 274 147 411 4696 Fragments arranged by size: 4696 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5701 Fragments arranged by size: 5701 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2094 2585 1186 Size: 908 491 4527 Fragments arranged by size: 4527 908 491 BseYI C'CCAG_C Cuts at: 2199 2621 5129 5625 2199 Size: 422 2508 496 2500 Fragments arranged by size: 2508 2500 496 422 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2225 2721 3101 1163 Size: 573 489 496 380 3988 Fragments arranged by size: 3988 573 496 489 380 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3376 4137 5165 1540 Size: 275 186 1375 761 1028 2301 Fragments arranged by size: 2301 1375 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2136 2439 2525 3188 3987 4072 5143 2136 Size: 303 86 663 799 85 1071 2919 Fragments arranged by size: 2919 1071 799 663 303 86 85 BsmI GAATG_Cn' Cuts at: 3437 3530 3437 Size: 93 5833 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2102 2136 2439 2525 2570 3188 3245 Size: 399 312 34 303 86 45 618 57 Cuts at: 3245 3987 4072 5143 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 618 399 312 303 86 85 57 45 34 BspEI T'CCGG_A Cuts at: 5589 5589 Size: 5926 BspHI T'CATG_A Cuts at: 3815 5545 3815 Size: 1730 4196 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2782 3281 844 Size: 1938 499 3489 Fragments arranged by size: 3489 1938 499 BsrBI CCG'CTC Cuts at: 1394 1823 2964 3813 1394 Size: 429 1141 849 3507 Fragments arranged by size: 3507 1141 849 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2839 4421 4595 65 Size: 1183 1591 1582 174 1396 Fragments arranged by size: 1591 1582 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2559 4570 4757 1700 Size: 110 749 2011 187 2869 Fragments arranged by size: 2869 2011 749 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5926 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5926 BssSI C'ACGA_G Cuts at: 2363 3986 4998 2363 Size: 1623 1012 3291 Fragments arranged by size: 3291 1623 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2434 794 Size: 1640 4286 BstBI TT'CG_AA Cuts at: 4730 4730 Size: 5926 BstEII G'GTnAC_C Cuts at: 1184 2948 1184 Size: 1764 4162 BstXI CCAn_nnnn'nTGG Cuts at: 2403 2403 Size: 5926 BstYI r'GATC_y Cuts at: 1972 2125 2307 2453 2509 2717 3126 3256 3623 Size: 153 182 146 56 208 409 130 367 Cuts at: 3623 4008 4025 5477 5563 5575 5921 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 409 385 367 346 208 182 153 146 130 86 56 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3217 513 Size: 552 939 1213 3222 Fragments arranged by size: 3222 1213 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5187 Fragments arranged by size: 5187 683 56 BtsI GCAGTG_nn' Cuts at: 2408 2709 3442 4236 4256 2408 Size: 301 733 794 20 4078 Fragments arranged by size: 4078 794 733 301 20 ClaI AT'CG_AT Cuts at: 1554 2329 1554 Size: 775 5151 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5891 DraI TTT'AAA Cuts at: 3230 3230 Size: 5926 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4933 809 Size: 507 3617 1802 Fragments arranged by size: 3617 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2409 3157 3373 Size: 54 1425 50 275 18 579 748 216 Cuts at: 3373 4262 5891 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 748 579 275 216 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3373 1537 Size: 275 1561 4090 Fragments arranged by size: 4090 1561 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2082 3023 3856 1353 Size: 729 941 833 3423 Fragments arranged by size: 3423 941 833 729 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4513 4887 5033 387 Size: 1064 138 2924 374 146 1280 Fragments arranged by size: 2924 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2134 2523 3186 2134 Size: 389 663 4874 Fragments arranged by size: 4874 663 389 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2064 2134 2511 3047 3131 3156 3989 Size: 393 432 70 377 536 84 25 833 Cuts at: 3989 4586 5373 5641 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 833 787 597 536 432 393 377 268 84 70 25 EcoO109I rG'GnC_Cy Cuts at: 2098 2099 2632 2098 Size: 1 533 5392 Fragments arranged by size: 5392 533 1 EcoRI G'AATT_C Cuts at: 3016 3235 3613 3016 Size: 219 378 5329 Fragments arranged by size: 5329 378 219 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2455 2455 Size: 5926 FspI TGC'GCA Cuts at: 4432 4432 Size: 5926 HaeII r_GCGC'y Cuts at: 1678 2195 5073 1678 Size: 517 2878 2531 Fragments arranged by size: 2878 2531 517 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2299 2331 2557 2589 4567 Size: 32 135 32 727 32 226 32 1978 Cuts at: 4567 4599 4641 4673 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1978 727 226 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3105 3270 3516 669 Size: 988 281 1167 165 246 3079 Fragments arranged by size: 3079 1167 988 281 246 165 HindIII A'AGCT_T Cuts at: 748 2046 3004 3172 748 Size: 1298 958 168 3502 Fragments arranged by size: 3502 1298 958 168 HpaI GTT'AAC Cuts at: 3516 3516 Size: 5926 KpnI G_GTAC'C Cuts at: 3251 3251 Size: 5926 MfeI C'AATT_G Cuts at: 3525 3525 Size: 5926 MluI A'CGCG_T Cuts at: 5802 5802 Size: 5926 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2061 2230 5040 5224 1305 Size: 756 169 2810 184 2007 Fragments arranged by size: 2810 2007 756 184 169 MscI TGG'CCA Cuts at: 10 64 1489 1832 3159 10 Size: 54 1425 343 1327 2777 Fragments arranged by size: 2777 1425 1327 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3151 3884 4243 4402 518 Size: 648 1985 733 359 159 2042 Fragments arranged by size: 2042 1985 733 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2711 3342 4019 5167 5412 1606 Size: 1105 631 677 1148 245 2120 Fragments arranged by size: 2120 1148 1105 677 631 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3217 513 Size: 552 939 1213 3222 Fragments arranged by size: 3222 1213 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5926 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5926 NotI GC'GGCC_GC Cuts at: 3373 3373 Size: 5926 NspI r_CATG'y Cuts at: 1132 1229 3284 4829 1132 Size: 97 2055 1545 2229 Fragments arranged by size: 2229 2055 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5926 PciI A'CATG_T Cuts at: 4825 4825 Size: 5926 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5926 PmeI GTTT'AAAC Cuts at: 3230 3230 Size: 5926 PmlI CAC'GTG Cuts at: 3012 3069 3012 Size: 57 5869 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3995 4027 5508 5540 3995 Size: 32 1481 32 4381 Fragments arranged by size: 4381 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5926 PsiI TTA'TAA Cuts at: 3496 3496 Size: 5926 PspOMI G'GGCC_C Cuts at: 2098 2098 Size: 5926 PstI C_TGCA'G Cuts at: 830 2739 3278 830 Size: 1909 539 3478 Fragments arranged by size: 3478 1909 539 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5926 PvuII CAG'CTG Cuts at: 2711 2711 Size: 5926 SacI G_AGCT'C Cuts at: 2136 2525 3188 2136 Size: 389 663 4874 Fragments arranged by size: 4874 663 389 SalI G'TCGA_C Cuts at: 1936 3268 1936 Size: 1332 4594 SbfI CC_TGCA'GG Cuts at: 3278 3278 Size: 5926 ScaI AGT'ACT Cuts at: 4174 5886 4174 Size: 1712 4214 SfcI C'TryA_G Cuts at: 826 1045 2735 3274 4409 5090 5281 826 Size: 219 1690 539 1135 681 191 1471 Fragments arranged by size: 1690 1471 1135 681 539 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5926 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5926 SmaI CCC'GGG Cuts at: 1872 3253 1872 Size: 1381 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4030 4931 5193 820 Size: 197 926 2087 901 262 1553 Fragments arranged by size: 2087 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3717 493 Size: 3224 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5926 SphI G_CATG'C Cuts at: 1132 3284 1132 Size: 2152 3774 SspI AAT'ATT Cuts at: 5 52 3850 5 Size: 47 3798 2081 Fragments arranged by size: 3798 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5926 StyI C'CwwG_G Cuts at: 513 1065 2004 2247 2501 3217 3365 513 Size: 552 939 243 254 716 148 3074 Fragments arranged by size: 3074 939 716 552 254 243 148 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2395 2895 3953 3970 4123 4308 4753 Size: 1255 815 500 1058 17 153 185 445 Cuts at: 4753 325 Size: 1498 Fragments arranged by size: 1498 1255 1058 815 500 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4172 5884 96 Size: 274 80 33 51 1443 2195 1712 138 Fragments arranged by size: 2195 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2369 2401 5886 5918 2369 Size: 32 3485 32 2377 Fragments arranged by size: 3485 2377 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5926 XbaI T'CTAG_A Cuts at: 3262 3262 Size: 5926 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5926 XmaI C'CCGG_G Cuts at: 1870 3251 1870 Size: 1381 4545 XmnI GAAnn'nnTTC Cuts at: 2373 3005 4055 2373 Size: 632 1050 4244 Fragments arranged by size: 4244 1050 632 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3734 276 Size: 53 83 186 936 2200 2468 Fragments arranged by size: 2468 2200 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI