(Circular) (Six-base) MAPSORT of: pFN21AB7966.seq Check: 8136 from: 1 to: 5908 pFN21AB7966 5908 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3718 278 Size: 53 83 186 936 2182 2468 Fragments arranged by size: 2468 2182 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2624 3251 1937 Size: 687 627 4594 Fragments arranged by size: 4594 687 627 Acc65I G'GTAC_C Cuts at: 3229 3229 Size: 5908 AclI AA'CG_TT Cuts at: 1563 4035 4408 1563 Size: 2472 373 3063 Fragments arranged by size: 3063 2472 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2565 3115 3173 3971 5355 5623 1632 Size: 933 550 58 798 1384 268 1917 Fragments arranged by size: 1917 1384 933 798 550 268 58 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5711 AflIII A'CryG_T Cuts at: 1203 4807 5784 1203 Size: 3604 977 1327 Fragments arranged by size: 3604 1327 977 AgeI A'CCGG_T Cuts at: 4739 4739 Size: 5908 AhdI GACnn_n'nnGTC Cuts at: 4637 4637 Size: 5908 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5908 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5908 AlwNI CAG_nnn'CTG Cuts at: 2423 3651 5223 2423 Size: 1228 1572 3108 Fragments arranged by size: 3108 1572 1228 ApaI G_GGCC'C Cuts at: 2316 2316 Size: 5908 ApaLI G'TGCA_C Cuts at: 3965 5121 3965 Size: 1156 4752 ApoI r'AATT_y Cuts at: 1493 3217 3443 3595 5704 1493 Size: 1724 226 152 2109 1697 Fragments arranged by size: 2109 1724 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4462 160 Size: 872 3430 1606 Fragments arranged by size: 3430 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5908 AvaI C'yCGr_G Cuts at: 1870 1943 2108 2878 3233 1870 Size: 73 165 770 355 4545 Fragments arranged by size: 4545 770 355 165 73 AvrII C'CTAG_G Cuts at: 2845 2845 Size: 5908 BamHI G'GATC_C Cuts at: 3238 3605 3238 Size: 367 5541 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2382 3229 4684 618 Size: 325 217 627 595 847 1455 1842 Fragments arranged by size: 1842 1455 847 627 595 325 217 BanII G_rGCy'C Cuts at: 1391 2045 2063 2316 2756 3227 1391 Size: 654 18 253 440 471 4072 Fragments arranged by size: 4072 654 471 440 253 18 BbeI G_GCGC'C Cuts at: 2386 2386 Size: 5908 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4937 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2522 2556 4099 4133 1903 Size: 34 585 34 1543 34 3678 Fragments arranged by size: 3678 1543 585 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3800 5010 3800 Size: 1210 4698 BclI T'GATC_A Cuts at: 1548 1548 Size: 5908 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2320 4519 136 Size: 107 122 71 631 605 648 2199 1525 Fragments arranged by size: 2199 1525 648 631 605 122 107 71 BglII A'GATC_T Cuts at: 5903 5903 Size: 5908 BlpI GC'TnA_GC Cuts at: 3325 3325 Size: 5908 Bme1580I G_kGCm'C Cuts at: 1790 2284 2316 3969 5125 1790 Size: 494 32 1653 1156 2573 Fragments arranged by size: 2573 1653 1156 494 32 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5908 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2113 3639 4597 Size: 416 53 83 399 24 696 1526 958 Cuts at: 4597 442 Size: 1753 Fragments arranged by size: 1753 1526 958 696 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5908 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4568 1239 Size: 3329 2579 Bpu10I CC'TnA_GC Cuts at: 2488 2865 2488 Size: 377 5531 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4033 4898 5196 4033 Size: 865 298 4745 Fragments arranged by size: 4745 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2798 4571 882 Size: 617 1299 1773 2219 Fragments arranged by size: 2219 1773 1299 617 BsaAI yAC'GTr Cuts at: 493 1204 3699 493 Size: 711 2495 2702 Fragments arranged by size: 2702 2495 711 BsaBI GATnn'nnATC Cuts at: 2706 2774 5902 2706 Size: 68 3128 2712 Fragments arranged by size: 3128 2712 68 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2147 2383 3715 4097 Size: 53 83 186 936 614 236 1332 382 Cuts at: 4097 4732 275 Size: 635 1451 Fragments arranged by size: 1451 1332 936 635 614 382 236 186 83 53 BsaWI w'CCGG_w Cuts at: 3112 4341 4739 5013 5160 5571 3112 Size: 1229 398 274 147 411 3449 Fragments arranged by size: 3449 1229 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5683 Fragments arranged by size: 5683 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2241 2367 2660 2816 3087 1186 Size: 1055 126 293 156 271 4007 Fragments arranged by size: 4007 1055 293 271 156 126 BseYI C'CCAG_C Cuts at: 2188 2296 2309 2607 2828 5111 5607 2188 Size: 108 13 298 221 2283 496 2489 Fragments arranged by size: 2489 2283 496 298 221 108 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2552 1163 Size: 573 816 4519 Fragments arranged by size: 4519 816 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2324 2967 3358 4119 5147 1540 Size: 275 186 323 643 391 761 1028 2301 Fragments arranged by size: 2301 1028 761 643 391 323 275 186 BsiHKAI G_wGCw'C Cuts at: 2126 2562 2756 3969 4054 5125 2126 Size: 436 194 1213 85 1071 2909 Fragments arranged by size: 2909 1213 1071 436 194 85 BsmI GAATG_Cn' Cuts at: 3419 3512 3419 Size: 93 5815 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2045 2063 2126 2284 2316 2562 2756 Size: 399 255 18 63 158 32 246 194 Cuts at: 2756 3227 3969 4054 5125 1391 Size: 471 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 471 399 255 246 194 158 85 63 32 18 BspEI T'CCGG_A Cuts at: 5571 5571 Size: 5908 BspHI T'CATG_A Cuts at: 3797 5527 3797 Size: 1730 4178 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3263 844 Size: 2419 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3795 1394 Size: 429 1972 3507 Fragments arranged by size: 3507 1972 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4403 4577 65 Size: 1183 3155 174 1396 Fragments arranged by size: 3155 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2257 2319 4552 4739 1700 Size: 110 447 62 2233 187 2869 Fragments arranged by size: 2869 2233 447 187 110 62 BsrGI T'GTAC_A Cuts at: 96 2822 96 Size: 2726 3182 BssHII G'CGCG_C Cuts at: 1925 2261 1925 Size: 336 5572 BssSI C'ACGA_G Cuts at: 3968 4980 3968 Size: 1012 4896 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5908 BstBI TT'CG_AA Cuts at: 4712 4712 Size: 5908 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5908 BstXI CCAn_nnnn'nTGG Cuts at: 2196 2196 Size: 5908 BstYI r'GATC_y Cuts at: 1972 2701 3238 3605 3990 4007 5459 5545 5557 Size: 729 537 367 385 17 1452 86 12 Cuts at: 5557 5903 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 729 537 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2090 2133 2164 2195 2342 2728 Size: 552 939 86 43 31 31 147 386 Cuts at: 2728 513 Size: 3693 Fragments arranged by size: 3693 939 552 386 147 86 43 31 31 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5169 Fragments arranged by size: 5169 683 56 BtsI GCAGTG_nn' Cuts at: 2434 3424 4218 4238 2434 Size: 990 794 20 4104 Fragments arranged by size: 4104 990 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5908 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5873 DraI TTT'AAA Cuts at: 3212 3212 Size: 5908 DraIII CAC_nnn'GTG Cuts at: 3080 3080 Size: 5908 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4915 809 Size: 507 3599 1802 Fragments arranged by size: 3599 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2321 3003 3355 Size: 54 1425 50 275 18 491 682 352 Cuts at: 3355 4244 5873 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 682 491 352 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2321 3355 1537 Size: 275 509 1034 4090 Fragments arranged by size: 4090 1034 509 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3838 1353 Size: 2485 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4495 4869 5015 387 Size: 1064 138 2906 374 146 1280 Fragments arranged by size: 2906 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2754 2754 Size: 5908 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2565 3115 3173 3971 4568 5355 5623 Size: 393 933 550 58 798 597 787 268 Cuts at: 5623 1239 Size: 1524 Fragments arranged by size: 1524 933 798 787 597 550 393 268 58 EcoNI CCTnn'n_nnAGG Cuts at: 2519 3122 2519 Size: 603 5305 EcoO109I rG'GnC_Cy Cuts at: 2232 2313 2232 Size: 81 5827 EcoRI G'AATT_C Cuts at: 3217 3595 3217 Size: 378 5530 FspI TGC'GCA Cuts at: 4414 4414 Size: 5908 HaeII r_GCGC'y Cuts at: 1678 2195 2386 5055 1678 Size: 517 191 2669 2531 Fragments arranged by size: 2669 2531 517 191 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4549 4581 4623 4655 1373 Size: 32 135 32 2977 32 42 32 2626 Fragments arranged by size: 2977 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3252 3498 669 Size: 988 281 1314 246 3079 Fragments arranged by size: 3079 1314 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5908 HpaI GTT'AAC Cuts at: 3498 3498 Size: 5908 KasI G'GCGC_C Cuts at: 2382 2382 Size: 5908 KpnI G_GTAC'C Cuts at: 3233 3233 Size: 5908 MfeI C'AATT_G Cuts at: 3507 3507 Size: 5908 MluI A'CGCG_T Cuts at: 5784 5784 Size: 5908 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5022 5206 1305 Size: 3717 184 2007 Fragments arranged by size: 3717 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4086 Fragments arranged by size: 4086 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3866 4225 4384 518 Size: 648 2700 359 159 2042 Fragments arranged by size: 2700 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2166 2344 2483 2828 3206 3324 4001 5149 Size: 560 178 139 345 378 118 677 1148 Cuts at: 5149 5394 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 560 378 345 245 178 139 118 NaeI GCC'GGC Cuts at: 2321 2321 Size: 5908 NarI GG'CG_CC Cuts at: 2383 2383 Size: 5908 NcoI C'CATG_G Cuts at: 513 1065 2004 2090 513 Size: 552 939 86 4331 Fragments arranged by size: 4331 939 552 86 NdeI CA'TA_TG Cuts at: 387 387 Size: 5908 NgoMIV G'CCGG_C Cuts at: 2319 2319 Size: 5908 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5908 NotI GC'GGCC_GC Cuts at: 3355 3355 Size: 5908 NspI r_CATG'y Cuts at: 1132 1229 2683 3266 4811 1132 Size: 97 1454 583 1545 2229 Fragments arranged by size: 2229 1545 1454 583 97 PasI CC'CwG_GG Cuts at: 1348 2356 1348 Size: 1008 4900 PciI A'CATG_T Cuts at: 4807 4807 Size: 5908 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5908 PfoI T'CCnGG_A Cuts at: 2391 2391 Size: 5908 PmeI GTTT'AAAC Cuts at: 3212 3212 Size: 5908 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3977 4009 5490 5522 3977 Size: 32 1481 32 4363 Fragments arranged by size: 4363 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2232 2232 Size: 5908 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5908 PsiI TTA'TAA Cuts at: 3478 3478 Size: 5908 PspOMI G'GGCC_C Cuts at: 2312 2312 Size: 5908 PstI C_TGCA'G Cuts at: 830 3178 3260 830 Size: 2348 82 3478 Fragments arranged by size: 3478 2348 82 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5908 PvuII CAG'CTG Cuts at: 2828 3206 2828 Size: 378 5530 SacI G_AGCT'C Cuts at: 2756 2756 Size: 5908 SacII CC_GC'GG Cuts at: 2167 2345 2167 Size: 178 5730 SalI G'TCGA_C Cuts at: 1936 3250 1936 Size: 1314 4594 SbfI CC_TGCA'GG Cuts at: 3260 3260 Size: 5908 ScaI AGT'ACT Cuts at: 4156 5868 4156 Size: 1712 4196 SfcI C'TryA_G Cuts at: 826 1045 2630 3174 3256 4391 5072 5263 826 Size: 219 1585 544 82 1135 681 191 1471 Fragments arranged by size: 1585 1471 1135 681 544 219 191 82 SfiI GGCCn_nnn'nGGCC Cuts at: 2320 2320 Size: 5908 SfoI GGC'GCC Cuts at: 2384 2384 Size: 5908 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5908 SgrAI Cr'CCGG_yG Cuts at: 1700 2257 1700 Size: 557 5351 SmaI CCC'GGG Cuts at: 1872 2110 2880 3235 1872 Size: 238 770 355 4545 Fragments arranged by size: 4545 770 355 238 SmlI C'TyrA_G Cuts at: 820 1017 1943 4012 4913 5175 820 Size: 197 926 2069 901 262 1553 Fragments arranged by size: 2069 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3699 493 Size: 3206 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5908 SphI G_CATG'C Cuts at: 1132 3266 1132 Size: 2134 3774 SspI AAT'ATT Cuts at: 5 52 3832 5 Size: 47 3780 2081 Fragments arranged by size: 3780 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5908 StyI C'CwwG_G Cuts at: 513 1065 2004 2090 2304 2463 2520 2845 3347 Size: 552 939 86 214 159 57 325 502 Cuts at: 3347 513 Size: 3074 Fragments arranged by size: 3074 939 552 502 325 214 159 86 57 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2953 3935 3952 4105 4290 4735 325 Size: 1255 1373 982 17 153 185 445 1498 Fragments arranged by size: 1498 1373 1255 982 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2822 4154 5866 Size: 274 80 33 51 1443 845 1332 1712 Cuts at: 5866 96 Size: 138 Fragments arranged by size: 1712 1443 1332 845 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5868 5900 5868 Size: 32 5876 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5908 XbaI T'CTAG_A Cuts at: 2797 3244 2797 Size: 447 5461 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2140 3131 2140 Size: 991 4917 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5908 XmaI C'CCGG_G Cuts at: 1870 2108 2878 3233 1870 Size: 238 770 355 4545 Fragments arranged by size: 4545 770 355 238 XmnI GAAnn'nnTTC Cuts at: 4037 4037 Size: 5908 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3716 276 Size: 53 83 186 936 2182 2468 Fragments arranged by size: 2468 2182 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI SapI SexAI SrfI SwaI