Restriction map for
pFN21AB7965
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII AclI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
AspEI AspI AvrII BamHI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BpuAI BsaBI
BsaI BseRI BsgI BsiEI BsiWI
BsmAI BsmBI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
Bst1107I BstAPI BstBI BstXI BstYI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
HgaI HindIII HpaI KspI MaeI
MamI MfeI MflI MluI MunI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
TfiI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 663 GGTACC G^GTACC
AccI 739 GTCTAC GT^[AC][GT]AC
AccIII 263 TCCGGA T^CCGGA
AcsI 24 AAATTC [GA]^AATT[CT]
AlwNI 89 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 768 GGGCCC GGGCC^C
ApoI 24 AAATTC [GA]^AATT[CT]
Asp718I 663 GGTACC G^GTACC
AvaII 514 GGACC G^G[AT]CC
AviII 404 TGCGCA TGC^GCA
BanII 768 GGGCCC G[GA]GC[CT]^C
BbrPI 378 CACGTG CAC^GTG
BglI 484 GCCTTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 297 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 297 GCTGAGC GC^T[ACGT]AGC
BsaAI 378 CACGTG [CT]AC^GT[GA]
BseAI 263 TCCGGA T^CCGGA
BsmI 213 GCATTC GAATGC(1/-1)
BspEI 263 TCCGGA T^CCGGA
BspMI 269 ACCTGC ACCTGC(4/8)
BssSI 559 CTCGTG CACGAG(-5/-1)
BstEII 360 GGTGACC G^GT[ACGT]ACC
BstPI 360 GGTGACC G^GT[ACGT]ACC
CelII 297 GCTGAGC GC^T[ACGT]AGC
DpnII 447 GATC ^GATC
EarI 137 GAAGAG CTCTTC(1/4)
EcoO65I 360 GGTGACC G^GT[ACGT]ACC
FspI 404 TGCGCA TGC^GCA
HincII 61 GTCAAC GT[CT]^[GA]AC
HindII 61 GTCAAC GT[CT]^[GA]AC
HinfI 814 GACTC G^A[ACGT]TC
KpnI 663 GGTACC GGTAC^C
Ksp632I 137 GAAGAG CTCTTC(1/4)
MaeII 379 ACGT A^CGT
MboI 447 GATC ^GATC
MroI 263 TCCGGA T^CCGGA
MseI 392 TTAA T^TAA
MslI 759 CACCTCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 334 GCCGGC GCC^GGC
NdeII 447 GATC ^GATC
NgoMIV 334 GCCGGC G^CCGGC
PleI 814 GACTC GAGTC(4/5)
PmaCI 378 CACGTG CAC^GTG
PmlI 378 CACGTG CAC^GTG
PpuMI 513 GGGACCC [GA]G^G[AT]CC[CT]
PstI 271 CTGCAG CTGCA^G
SapI 137 GAAGAGC GCTCTTC(1/4)
Sau3AI 447 GATC ^GATC
SfcI 271 CTGCAG C^T[GA][CT]AG
Tru9I 392 TTAA T^TAA
Tsp509I 25 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 327 GTAC GT^AC
664 GTAC
AspHI 97 GAGCAC G[AT]GC[AT]^C
300 GAGCAC
AvaI 466 CCCGGG C^[CT]CG[GA]G
571 CCCGGG
BalI 428 TGGCCA TGG^CCA
678 TGGCCA
BpmI 223 CTGGAG CTGGAG(16/14)
626 CTGGAG
BsaWI 72 ACCGGA [AT]^CCGG[AT]
263 TCCGGA
BsiHKAI 97 GAGCAC G[AT]GC[AT]^C
300 GAGCAC
BsmFI 513 GGGAC GGGAC(10/14)
33 GTCCC
BsoBI 466 CCCGGG C^[CT]CG[GA]G
571 CCCGGG
BsrFI 334 GCCGGC [GA]^CCGG[CT]
666 ACCGGC
BsrI 56 CCAGT ACTGG(1/-1)
681 CCAGT
Cfr10I 334 GCCGGC [GA]^CCGG[CT]
666 ACCGGC
DsaI 764 CCGTGG C^C[GA][CT]GG
829 CCGTGG
HphI 360 GGTGA GGTGA(8/7)
784 TCACC
MaeIII 361 GTGAC ^GT[ACGT]AC
783 GTCAC
MboII 137 GAAGA GAAGA(8/7)
195 GAAGA
MluNI 428 TGGCCA TGG^CCA
678 TGGCCA
MscI 428 TGGCCA TGG^CCA
678 TGGCCA
PvuII 5 CAGCTG CAG^CTG
166 CAGCTG
RsaI 327 GTAC GT^AC
664 GTAC
SfaNI 187 GATGC GCATC(5/9)
91 GCATC
SmaI 466 CCCGGG CCC^GGG
571 CCCGGG
Tsp45I 361 GTGAC ^GT[GC]AC
783 GTCAC
XmaCI 466 CCCGGG C^CCGGG
571 CCCGGG
XmaI 466 CCCGGG C^CCGGG
571 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AccII AciI AcyI AluI BanI
BbeI BbvI BcnI BmyI BsaHI
BsaJI BsiYI BslI Bsp1286I BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DraII EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HpaII ItaI KasI MnlI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NlaIII NlaIV Sau96I ScrFI
SfoI TseI TspRI