Restriction map for pFN21AB7965
 (Circular) (Six-base) MAPSORT of: pFN21AB7965.seq  Check: 2416  from: 1  to: 5545



pFN21AB7965  5545 bp

 With 182 enzymes: SgfI * 

                        April 15, 2010 09:29  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3355     278 
   Size:         53      83     186     936    1819    2468

  Fragments arranged by size: 

                2468    1819     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2745    2888    1937 
   Size:        808     143    4594

  Fragments arranged by size: 

                4594     808     143
 
Acc65I G'GTAC_C

Cuts at:   2668    2866    2668 
   Size:        198    5347
 
AclI AA'CG_TT

Cuts at:   1563    2844    3672    4045    1563 
   Size:       1281     828     373    3063

  Fragments arranged by size: 

                3063    1281     828     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2219    2329    3608    4992    5260    1632 
   Size:        587     110    1279    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1279     587     268     110
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5348
 
AflIII A'CryG_T

Cuts at:   1203    4444    5421    1203 
   Size:       3241     977    1327

  Fragments arranged by size: 

                3241    1327     977
 
AgeI A'CCGG_T

Cuts at:   4376    4376 
   Size:       5545
 
AhdI GACnn_n'nnGTC

Cuts at:   4274    4274 
   Size:       5545
 
AleI CACnn'nnGTG

Cuts at:   1166    2768    1166 
   Size:       1602    3943
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2435    1184 
   Size:       1251    4294
 
AlwNI CAG_nnn'CTG

Cuts at:   2099    3288    4860    2099 
   Size:       1189    1572    2784

  Fragments arranged by size: 

                2784    1572    1189
 
ApaI G_GGCC'C

Cuts at:   2777    2777 
   Size:       5545
 
ApaLI G'TGCA_C

Cuts at:   3602    4758    3602 
   Size:       1156    4389
 
ApoI r'AATT_y

Cuts at:   1493    2029    2854    3080    3232    5341    1493 
   Size:        536     825     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     825     536     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4099     160 
   Size:        872    3067    1606

  Fragments arranged by size: 

                3067    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5545
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2471    2576    2870    1870 
   Size:         73     528     105     294    4545

  Fragments arranged by size: 

                4545     528     294     105      73
 
BamHI G'GATC_C

Cuts at:   2875    3242    2875 
   Size:        367    5178
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2497    2587    2603    2668    2762 
   Size:        325     217     627     710      90      16      65      94

Cuts at:   2762    2866    4321     618 
   Size:        104    1455    1842

  Fragments arranged by size: 

                1842    1455     710     627     325     217     104      94
                  90      65      16
 
BanII G_rGCy'C

Cuts at:   1391    2777    2864    1391 
   Size:       1386      87    4072

  Fragments arranged by size: 

                4072    1386      87
 
BbeI G_GCGC'C

Cuts at:   2501    2591    2607    2501 
   Size:         90      16    5439

  Fragments arranged by size: 

                5439      90      16
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4574
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    1987    2021    3736    3770    1903 
   Size:         34      50      34    1715      34    3678

  Fragments arranged by size: 

                3678    1715      50      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3437    4647    3437 
   Size:       1210    4335
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5545
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2495    4156     136 
   Size:        107     122      71     631     605     823    1661    1525

  Fragments arranged by size: 

                1661    1525     823     631     605     122     107      71
            
 
BglII A'GATC_T

Cuts at:   5540    5540 
   Size:       5545
 
BlpI GC'TnA_GC

Cuts at:   2303    2962    2303 
   Size:        659    4886
 
Bme1580I G_kGCm'C

Cuts at:   1790    2571    2777    3606    4762    1790 
   Size:        781     206     829    1156    2573

  Fragments arranged by size: 

                2573    1156     829     781     206
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5545
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3276    4234     442 
   Size:        416      53      83     399      24    1859     958    1753

  Fragments arranged by size: 

                1859    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5545
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2249    2652    4205    1239 
   Size:       1010     403    1553    2579

  Fragments arranged by size: 

                2579    1553    1010     403
 
Bpu10I CC'TnA_GC

Cuts at:   2643    2643 
   Size:       5545
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3670    4535    4833    3670 
   Size:        865     298    4382

  Fragments arranged by size: 

                4382     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4208     882 
   Size:        617    2709    2219

  Fragments arranged by size: 

                2709    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2385    3336     493 
   Size:        711    1181     951    2702

  Fragments arranged by size: 

                2702    1181     951     711
 
BsaBI GATnn'nnATC

Cuts at:   5539    5539 
   Size:       5545
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2498    2588    2604    3352 
   Size:         53      83     186     936     965      90      16     748

Cuts at:   3352    3734    4369     275 
   Size:        382     635    1451

  Fragments arranged by size: 

                1451     965     936     748     635     382     186      90
                  83      53      16
 
BsaWI w'CCGG_w

Cuts at:   2077    2268    3978    4376    4650    4797    5208    2077 
   Size:        191    1710     398     274     147     411    2414

  Fragments arranged by size: 

                2414    1710     411     398     274     191     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5320

  Fragments arranged by size: 

                5320     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5545
 
BseYI C'CCAG_C

Cuts at:   2056    2323    2532    2617    4748    5244    2056 
   Size:        267     209      85    2131     496    2357

  Fragments arranged by size: 

                2357    2131     496     267     209      85
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4972
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2995    3756    4784    1540 
   Size:        275     186     994     761    1028    2301

  Fragments arranged by size: 

                2301    1028     994     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2106    2309    3606    3691    4762    2106 
   Size:        203    1297      85    1071    2889

  Fragments arranged by size: 

                2889    1297    1071     203      85
 
BsmI GAATG_Cn'

Cuts at:   2218    3056    3149    2218 
   Size:        838      93    4614

  Fragments arranged by size: 

                4614     838      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2106    2309    2571    2777    2864    3606    3691 
   Size:        399     316     203     262     206      87     742      85

Cuts at:   3691    4762    1391 
   Size:       1071    2174

  Fragments arranged by size: 

                2174    1071     742     399     316     262     206     203
                  87      85
 
BspEI T'CCGG_A

Cuts at:   2268    5208    2268 
   Size:       2940    2605
 
BspHI T'CATG_A

Cuts at:   3434    5164    3434 
   Size:       1730    3815
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2283    2900     844 
   Size:       1439     617    3489

  Fragments arranged by size: 

                3489    1439     617
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3432    1394 
   Size:        429    1609    3507

  Fragments arranged by size: 

                3507    1609     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4040    4214      65 
   Size:       1183    2792     174    1396

  Fragments arranged by size: 

                2792    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2339    2671    4189    4376    1700 
   Size:        110     529     332    1518     187    2869

  Fragments arranged by size: 

                2869    1518     529     332     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5545
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5545
 
BssSI C'ACGA_G

Cuts at:   2564    3605    4617    2564 
   Size:       1041    1012    3492

  Fragments arranged by size: 

                3492    1041    1012
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5545
 
BstBI TT'CG_AA

Cuts at:   4349    4349 
   Size:       5545
 
BstEII G'GTnAC_C

Cuts at:   1184    2365    1184 
   Size:       1181    4364
 
BstYI r'GATC_y

Cuts at:   1972    2875    3242    3627    3644    5096    5182    5194    5540 
   Size:        903     367     385      17    1452      86      12     346

Cuts at:   5540    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     903     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065    2769    2834     513 
   Size:        552    1704      65    3224

  Fragments arranged by size: 

                3224    1704     552      65
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4806

  Fragments arranged by size: 

                4806     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2790    3061    3855    3875    2790 
   Size:        271     794      20    4460

  Fragments arranged by size: 

                4460     794     271      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5545
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5510
 
DraI TTT'AAA

Cuts at:   2849    2849 
   Size:       5545
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4552     809 
   Size:        507    3236    1802

  Fragments arranged by size: 

                3236    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2425    2433    2683 
   Size:         54    1425      50     275      18     595       8     250

Cuts at:   2683    2992    3881    5510       8 
   Size:        309     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     595     309     275     250      54
                  50      43      18       8
 
EagI C'GGCC_G

Cuts at:   1537    1812    2992    1537 
   Size:        275    1180    4090

  Fragments arranged by size: 

                4090    1180     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2137    3475    1353 
   Size:        784    1338    3423

  Fragments arranged by size: 

                3423    1338     784
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2252    2831    2854    4132    4506    4652 
   Size:       1064     138     663     579      23    1278     374     146

Cuts at:   4652     387 
   Size:       1280

  Fragments arranged by size: 

                1280    1278    1064     663     579     374     146     138
                  23
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2219    2249    2329    2652    3608    4205    4992 
   Size:        393     587      30      80     323     956     597     787

Cuts at:   4992    5260    1239 
   Size:        268    1524

  Fragments arranged by size: 

                1524     956     787     597     587     393     323     268
                  80      30
 
EcoO109I rG'GnC_Cy

Cuts at:   2519    2712    2774    2519 
   Size:        193      62    5290

  Fragments arranged by size: 

                5290     193      62
 
EcoRI G'AATT_C

Cuts at:   2854    3232    2854 
   Size:        378    5167
 
FspI TGC'GCA

Cuts at:   2411    4051    2411 
   Size:       1640    3905
 
FspAI rTGC'GCAy

Cuts at:   2411    2411 
   Size:       5545
 
HaeII r_GCGC'y

Cuts at:   1678    2501    2591    2607    4692    1678 
   Size:        823      90      16    2085    2531

  Fragments arranged by size: 

                2531    2085     823      90      16
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    4186    4218    4260    4292    1373 
   Size:         32     135      32    2614      32      42      32    2626

  Fragments arranged by size: 

                2626    2614     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2068    2889    3135     669 
   Size:        988     281     130     821     246    3079

  Fragments arranged by size: 

                3079     988     821     281     246     130
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5545
 
HpaI GTT'AAC

Cuts at:   3135    3135 
   Size:       5545
 
KasI G'GCGC_C

Cuts at:   2497    2587    2603    2497 
   Size:         90      16    5439

  Fragments arranged by size: 

                5439      90      16
 
KpnI G_GTAC'C

Cuts at:   2672    2870    2672 
   Size:        198    5347
 
MfeI C'AATT_G

Cuts at:   3144    3144 
   Size:       5545
 
MluI A'CGCG_T

Cuts at:   5421    5421 
   Size:       5545
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4659    4843    1305 
   Size:       3354     184    2007

  Fragments arranged by size: 

                3354    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2435    2685      10 
   Size:         54    1425     343     603     250    2870

  Fragments arranged by size: 

                2870    1425     603     343     250      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2768    3503    3862    4021     518 
   Size:        648    1602     735     359     159    2042

  Fragments arranged by size: 

                2042    1602     735     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2012    2173    2281    2727    2760    2799    2961    3638 
   Size:        406     161     108     446      33      39     162     677

Cuts at:   3638    4786    5031    1606 
   Size:       1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     446     406     245     162     161
                 108      39      33
 
NaeI GCC'GGC

Cuts at:   2341    2341 
   Size:       5545
 
NarI GG'CG_CC

Cuts at:   2498    2588    2604    2498 
   Size:         90      16    5439

  Fragments arranged by size: 

                5439      90      16
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    4993
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5545
 
NgoMIV G'CCGG_C

Cuts at:   2339    2339 
   Size:       5545
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5545
 
NotI GC'GGCC_GC

Cuts at:   2992    2992 
   Size:       5545
 
NspI r_CATG'y

Cuts at:   1132    1229    2903    4448    1132 
   Size:         97    1674    1545    2229

  Fragments arranged by size: 

                2229    1674    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5545
 
PciI A'CATG_T

Cuts at:   4444    4444 
   Size:       5545
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5545
 
PmeI GTTT'AAAC

Cuts at:   2849    2849 
   Size:       5545
 
PmlI CAC'GTG

Cuts at:   2385    2385 
   Size:       5545
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3614    3646    5127    5159    3614 
   Size:         32    1481      32    4000

  Fragments arranged by size: 

                4000    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2519    2519 
   Size:       5545
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5545
 
PsiI TTA'TAA

Cuts at:   3115    3115 
   Size:       5545
 
PspOMI G'GGCC_C

Cuts at:   2773    2773 
   Size:       5545
 
PstI C_TGCA'G

Cuts at:    830    2280    2897     830 
   Size:       1450     617    3478

  Fragments arranged by size: 

                3478    1450     617
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5545
 
PvuII CAG'CTG

Cuts at:   2012    2173    2012 
   Size:        161    5384
 
SalI G'TCGA_C

Cuts at:   1936    2887    1936 
   Size:        951    4594
 
SanDI GG'GwC_CC

Cuts at:   2519    2519 
   Size:       5545
 
SapI GCTCTTCn'nnn_

Cuts at:   2137    2137 
   Size:       5545
 
SbfI CC_TGCA'GG

Cuts at:   2897    2897 
   Size:       5545
 
ScaI AGT'ACT

Cuts at:   3793    5505    3793 
   Size:       1712    3833
 
SfcI C'TryA_G

Cuts at:    826    1045    2276    2893    4028    4709    4900     826 
   Size:        219    1231     617    1135     681     191    1471

  Fragments arranged by size: 

                1471    1231    1135     681     617     219     191
 
SfoI GGC'GCC

Cuts at:   2499    2589    2605    2499 
   Size:         90      16    5439

  Fragments arranged by size: 

                5439      90      16
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5545
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5545
 
SmaI CCC'GGG

Cuts at:   1872    2473    2578    2872    1872 
   Size:        601     105     294    4545

  Fragments arranged by size: 

                4545     601     294     105
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3649    4550    4812     820 
   Size:        197     926    1706     901     262    1553

  Fragments arranged by size: 

                1706    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3336     493 
   Size:       2843    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5545
 
SphI G_CATG'C

Cuts at:   1132    2903    1132 
   Size:       1771    3774
 
SspI AAT'ATT

Cuts at:      5      52    3469       5 
   Size:         47    3417    2081

  Fragments arranged by size: 

                3417    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5545
 
StyI C'CwwG_G

Cuts at:    513    1065    2984     513 
   Size:        552    1919    3074

  Fragments arranged by size: 

                3074    1919     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3572    3589    3742    3927    4372     325 
   Size:       1255    1992      17     153     185     445    1498

  Fragments arranged by size: 

                1992    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3791    5503      96 
   Size:        274      80      33      51    1443    1814    1712     138

  Fragments arranged by size: 

                1814    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2810    2842    5505    5537    2810 
   Size:         32    2663      32    2818

  Fragments arranged by size: 

                2818    2663      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5545
 
XbaI T'CTAG_A

Cuts at:   2881    2881 
   Size:       5545
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5545
 
XmaI C'CCGG_G

Cuts at:   1870    2471    2576    2870    1870 
   Size:        601     105     294    4545

  Fragments arranged by size: 

                4545     601     294     105
 
XmnI GAAnn'nnTTC

Cuts at:   3674    3674 
   Size:       5545
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3353     276 
   Size:         53      83     186     936    1819    2468

  Fragments arranged by size: 

                2468    1819     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbeI     BbsI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI EcoO109I    EcoRI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NspI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI     PstI     PvuI    PvuII
     SalI    SanDI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     AscI    AvrII     BaeI    BbvCI    BfrBI
     BplI    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I   DraIII  EcoICRI
    EcoNI    EcoRV     FalI     FseI     NruI     NsiI     PacI     PfoI
    PspXI     PsrI    RsrII     SacI    SacII    SexAI     SfiI     SrfI
     SwaI     XcmI
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