(Circular) (Six-base) MAPSORT of: pFN21AB7965.seq Check: 2416 from: 1 to: 5545 pFN21AB7965 5545 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3355 278 Size: 53 83 186 936 1819 2468 Fragments arranged by size: 2468 1819 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2745 2888 1937 Size: 808 143 4594 Fragments arranged by size: 4594 808 143 Acc65I G'GTAC_C Cuts at: 2668 2866 2668 Size: 198 5347 AclI AA'CG_TT Cuts at: 1563 2844 3672 4045 1563 Size: 1281 828 373 3063 Fragments arranged by size: 3063 1281 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2219 2329 3608 4992 5260 1632 Size: 587 110 1279 1384 268 1917 Fragments arranged by size: 1917 1384 1279 587 268 110 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5348 AflIII A'CryG_T Cuts at: 1203 4444 5421 1203 Size: 3241 977 1327 Fragments arranged by size: 3241 1327 977 AgeI A'CCGG_T Cuts at: 4376 4376 Size: 5545 AhdI GACnn_n'nnGTC Cuts at: 4274 4274 Size: 5545 AleI CACnn'nnGTG Cuts at: 1166 2768 1166 Size: 1602 3943 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2435 1184 Size: 1251 4294 AlwNI CAG_nnn'CTG Cuts at: 2099 3288 4860 2099 Size: 1189 1572 2784 Fragments arranged by size: 2784 1572 1189 ApaI G_GGCC'C Cuts at: 2777 2777 Size: 5545 ApaLI G'TGCA_C Cuts at: 3602 4758 3602 Size: 1156 4389 ApoI r'AATT_y Cuts at: 1493 2029 2854 3080 3232 5341 1493 Size: 536 825 226 152 2109 1697 Fragments arranged by size: 2109 1697 825 536 226 152 AseI AT'TA_AT Cuts at: 160 1032 4099 160 Size: 872 3067 1606 Fragments arranged by size: 3067 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5545 AvaI C'yCGr_G Cuts at: 1870 1943 2471 2576 2870 1870 Size: 73 528 105 294 4545 Fragments arranged by size: 4545 528 294 105 73 BamHI G'GATC_C Cuts at: 2875 3242 2875 Size: 367 5178 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2497 2587 2603 2668 2762 Size: 325 217 627 710 90 16 65 94 Cuts at: 2762 2866 4321 618 Size: 104 1455 1842 Fragments arranged by size: 1842 1455 710 627 325 217 104 94 90 65 16 BanII G_rGCy'C Cuts at: 1391 2777 2864 1391 Size: 1386 87 4072 Fragments arranged by size: 4072 1386 87 BbeI G_GCGC'C Cuts at: 2501 2591 2607 2501 Size: 90 16 5439 Fragments arranged by size: 5439 90 16 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4574 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3736 3770 1903 Size: 34 50 34 1715 34 3678 Fragments arranged by size: 3678 1715 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3437 4647 3437 Size: 1210 4335 BclI T'GATC_A Cuts at: 1548 1548 Size: 5545 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2495 4156 136 Size: 107 122 71 631 605 823 1661 1525 Fragments arranged by size: 1661 1525 823 631 605 122 107 71 BglII A'GATC_T Cuts at: 5540 5540 Size: 5545 BlpI GC'TnA_GC Cuts at: 2303 2962 2303 Size: 659 4886 Bme1580I G_kGCm'C Cuts at: 1790 2571 2777 3606 4762 1790 Size: 781 206 829 1156 2573 Fragments arranged by size: 2573 1156 829 781 206 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5545 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3276 4234 442 Size: 416 53 83 399 24 1859 958 1753 Fragments arranged by size: 1859 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5545 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2249 2652 4205 1239 Size: 1010 403 1553 2579 Fragments arranged by size: 2579 1553 1010 403 Bpu10I CC'TnA_GC Cuts at: 2643 2643 Size: 5545 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3670 4535 4833 3670 Size: 865 298 4382 Fragments arranged by size: 4382 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4208 882 Size: 617 2709 2219 Fragments arranged by size: 2709 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2385 3336 493 Size: 711 1181 951 2702 Fragments arranged by size: 2702 1181 951 711 BsaBI GATnn'nnATC Cuts at: 5539 5539 Size: 5545 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2498 2588 2604 3352 Size: 53 83 186 936 965 90 16 748 Cuts at: 3352 3734 4369 275 Size: 382 635 1451 Fragments arranged by size: 1451 965 936 748 635 382 186 90 83 53 16 BsaWI w'CCGG_w Cuts at: 2077 2268 3978 4376 4650 4797 5208 2077 Size: 191 1710 398 274 147 411 2414 Fragments arranged by size: 2414 1710 411 398 274 191 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5320 Fragments arranged by size: 5320 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5545 BseYI C'CCAG_C Cuts at: 2056 2323 2532 2617 4748 5244 2056 Size: 267 209 85 2131 496 2357 Fragments arranged by size: 2357 2131 496 267 209 85 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4972 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2995 3756 4784 1540 Size: 275 186 994 761 1028 2301 Fragments arranged by size: 2301 1028 994 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2106 2309 3606 3691 4762 2106 Size: 203 1297 85 1071 2889 Fragments arranged by size: 2889 1297 1071 203 85 BsmI GAATG_Cn' Cuts at: 2218 3056 3149 2218 Size: 838 93 4614 Fragments arranged by size: 4614 838 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2106 2309 2571 2777 2864 3606 3691 Size: 399 316 203 262 206 87 742 85 Cuts at: 3691 4762 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 316 262 206 203 87 85 BspEI T'CCGG_A Cuts at: 2268 5208 2268 Size: 2940 2605 BspHI T'CATG_A Cuts at: 3434 5164 3434 Size: 1730 3815 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2283 2900 844 Size: 1439 617 3489 Fragments arranged by size: 3489 1439 617 BsrBI CCG'CTC Cuts at: 1394 1823 3432 1394 Size: 429 1609 3507 Fragments arranged by size: 3507 1609 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4040 4214 65 Size: 1183 2792 174 1396 Fragments arranged by size: 2792 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2339 2671 4189 4376 1700 Size: 110 529 332 1518 187 2869 Fragments arranged by size: 2869 1518 529 332 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5545 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5545 BssSI C'ACGA_G Cuts at: 2564 3605 4617 2564 Size: 1041 1012 3492 Fragments arranged by size: 3492 1041 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5545 BstBI TT'CG_AA Cuts at: 4349 4349 Size: 5545 BstEII G'GTnAC_C Cuts at: 1184 2365 1184 Size: 1181 4364 BstYI r'GATC_y Cuts at: 1972 2875 3242 3627 3644 5096 5182 5194 5540 Size: 903 367 385 17 1452 86 12 346 Cuts at: 5540 1972 Size: 1977 Fragments arranged by size: 1977 1452 903 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2769 2834 513 Size: 552 1704 65 3224 Fragments arranged by size: 3224 1704 552 65 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4806 Fragments arranged by size: 4806 683 56 BtsI GCAGTG_nn' Cuts at: 2790 3061 3855 3875 2790 Size: 271 794 20 4460 Fragments arranged by size: 4460 794 271 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5545 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5510 DraI TTT'AAA Cuts at: 2849 2849 Size: 5545 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4552 809 Size: 507 3236 1802 Fragments arranged by size: 3236 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2425 2433 2683 Size: 54 1425 50 275 18 595 8 250 Cuts at: 2683 2992 3881 5510 8 Size: 309 889 1629 43 Fragments arranged by size: 1629 1425 889 595 309 275 250 54 50 43 18 8 EagI C'GGCC_G Cuts at: 1537 1812 2992 1537 Size: 275 1180 4090 Fragments arranged by size: 4090 1180 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2137 3475 1353 Size: 784 1338 3423 Fragments arranged by size: 3423 1338 784 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2252 2831 2854 4132 4506 4652 Size: 1064 138 663 579 23 1278 374 146 Cuts at: 4652 387 Size: 1280 Fragments arranged by size: 1280 1278 1064 663 579 374 146 138 23 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2219 2249 2329 2652 3608 4205 4992 Size: 393 587 30 80 323 956 597 787 Cuts at: 4992 5260 1239 Size: 268 1524 Fragments arranged by size: 1524 956 787 597 587 393 323 268 80 30 EcoO109I rG'GnC_Cy Cuts at: 2519 2712 2774 2519 Size: 193 62 5290 Fragments arranged by size: 5290 193 62 EcoRI G'AATT_C Cuts at: 2854 3232 2854 Size: 378 5167 FspI TGC'GCA Cuts at: 2411 4051 2411 Size: 1640 3905 FspAI rTGC'GCAy Cuts at: 2411 2411 Size: 5545 HaeII r_GCGC'y Cuts at: 1678 2501 2591 2607 4692 1678 Size: 823 90 16 2085 2531 Fragments arranged by size: 2531 2085 823 90 16 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4186 4218 4260 4292 1373 Size: 32 135 32 2614 32 42 32 2626 Fragments arranged by size: 2626 2614 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2068 2889 3135 669 Size: 988 281 130 821 246 3079 Fragments arranged by size: 3079 988 821 281 246 130 HindIII A'AGCT_T Cuts at: 748 748 Size: 5545 HpaI GTT'AAC Cuts at: 3135 3135 Size: 5545 KasI G'GCGC_C Cuts at: 2497 2587 2603 2497 Size: 90 16 5439 Fragments arranged by size: 5439 90 16 KpnI G_GTAC'C Cuts at: 2672 2870 2672 Size: 198 5347 MfeI C'AATT_G Cuts at: 3144 3144 Size: 5545 MluI A'CGCG_T Cuts at: 5421 5421 Size: 5545 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4659 4843 1305 Size: 3354 184 2007 Fragments arranged by size: 3354 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2435 2685 10 Size: 54 1425 343 603 250 2870 Fragments arranged by size: 2870 1425 603 343 250 54 MslI CAynn'nnrTG Cuts at: 518 1166 2768 3503 3862 4021 518 Size: 648 1602 735 359 159 2042 Fragments arranged by size: 2042 1602 735 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2012 2173 2281 2727 2760 2799 2961 3638 Size: 406 161 108 446 33 39 162 677 Cuts at: 3638 4786 5031 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 446 406 245 162 161 108 39 33 NaeI GCC'GGC Cuts at: 2341 2341 Size: 5545 NarI GG'CG_CC Cuts at: 2498 2588 2604 2498 Size: 90 16 5439 Fragments arranged by size: 5439 90 16 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4993 NdeI CA'TA_TG Cuts at: 387 387 Size: 5545 NgoMIV G'CCGG_C Cuts at: 2339 2339 Size: 5545 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5545 NotI GC'GGCC_GC Cuts at: 2992 2992 Size: 5545 NspI r_CATG'y Cuts at: 1132 1229 2903 4448 1132 Size: 97 1674 1545 2229 Fragments arranged by size: 2229 1674 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5545 PciI A'CATG_T Cuts at: 4444 4444 Size: 5545 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5545 PmeI GTTT'AAAC Cuts at: 2849 2849 Size: 5545 PmlI CAC'GTG Cuts at: 2385 2385 Size: 5545 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3614 3646 5127 5159 3614 Size: 32 1481 32 4000 Fragments arranged by size: 4000 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2519 2519 Size: 5545 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5545 PsiI TTA'TAA Cuts at: 3115 3115 Size: 5545 PspOMI G'GGCC_C Cuts at: 2773 2773 Size: 5545 PstI C_TGCA'G Cuts at: 830 2280 2897 830 Size: 1450 617 3478 Fragments arranged by size: 3478 1450 617 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5545 PvuII CAG'CTG Cuts at: 2012 2173 2012 Size: 161 5384 SalI G'TCGA_C Cuts at: 1936 2887 1936 Size: 951 4594 SanDI GG'GwC_CC Cuts at: 2519 2519 Size: 5545 SapI GCTCTTCn'nnn_ Cuts at: 2137 2137 Size: 5545 SbfI CC_TGCA'GG Cuts at: 2897 2897 Size: 5545 ScaI AGT'ACT Cuts at: 3793 5505 3793 Size: 1712 3833 SfcI C'TryA_G Cuts at: 826 1045 2276 2893 4028 4709 4900 826 Size: 219 1231 617 1135 681 191 1471 Fragments arranged by size: 1471 1231 1135 681 617 219 191 SfoI GGC'GCC Cuts at: 2499 2589 2605 2499 Size: 90 16 5439 Fragments arranged by size: 5439 90 16 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5545 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5545 SmaI CCC'GGG Cuts at: 1872 2473 2578 2872 1872 Size: 601 105 294 4545 Fragments arranged by size: 4545 601 294 105 SmlI C'TyrA_G Cuts at: 820 1017 1943 3649 4550 4812 820 Size: 197 926 1706 901 262 1553 Fragments arranged by size: 1706 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3336 493 Size: 2843 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5545 SphI G_CATG'C Cuts at: 1132 2903 1132 Size: 1771 3774 SspI AAT'ATT Cuts at: 5 52 3469 5 Size: 47 3417 2081 Fragments arranged by size: 3417 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5545 StyI C'CwwG_G Cuts at: 513 1065 2984 513 Size: 552 1919 3074 Fragments arranged by size: 3074 1919 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3572 3589 3742 3927 4372 325 Size: 1255 1992 17 153 185 445 1498 Fragments arranged by size: 1992 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3791 5503 96 Size: 274 80 33 51 1443 1814 1712 138 Fragments arranged by size: 1814 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2810 2842 5505 5537 2810 Size: 32 2663 32 2818 Fragments arranged by size: 2818 2663 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5545 XbaI T'CTAG_A Cuts at: 2881 2881 Size: 5545 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5545 XmaI C'CCGG_G Cuts at: 1870 2471 2576 2870 1870 Size: 601 105 294 4545 Fragments arranged by size: 4545 601 294 105 XmnI GAAnn'nnTTC Cuts at: 3674 3674 Size: 5545 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3353 276 Size: 53 83 186 936 1819 2468 Fragments arranged by size: 2468 1819 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SacII SexAI SfiI SrfI SwaI XcmI