Restriction map for
pFN21AB7955
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# NO CUT ENZYMES ( 178 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwI AlwNI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaII AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmFI BsmI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstUI BstYI BstZ17I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FokI FseI
FspI HaeII HhaI Hin1I HinP1I
HindIII HpaI KasI KpnI KspI
MaeI MaeII MamI MfeI MflI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspBI SwaI TaqI
Tth111I TthHB8I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AflIII 45 ACATGT A^C[GA][CT]GT
AocI 87 CCTCAGG CC^T[ACGT]AGG
Asp700I 648 GAAGGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 506 CCCGAG C^[CT]CG[GA]G
BsaI 553 GGTCTC GGTCTC(1/5)
BsaJI 61 CCTTGG C^C[ACGT][ACGT]GG
BsaWI 702 ACCGGA [AT]^CCGG[AT]
BsoBI 506 CCCGAG C^[CT]CG[GA]G
BspLU11I 45 ACATGT A^CATGT
BspMI 186 ACCTGC ACCTGC(4/8)
BssKI 425 CCTGG ^CC[ACGT]GG
BstNI 425 CCTGG CC^[AT]GG
BstXI 688 CCAGCGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 87 CCTCAGG CC^T[ACGT]AGG
DraIII 367 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 556 CTCTTC CTCTTC(1/4)
Eco81I 87 CCTCAGG CC^T[ACGT]AGG
EcoRII 425 CCTGG ^CC[AT]GG
EcoT14I 61 CCTTGG C^C[AT][AT]GG
HapII 703 CCGG C^CGG
HincII 598 GTCAAC GT[CT]^[GA]AC
HindII 598 GTCAAC GT[CT]^[GA]AC
HpaII 703 CCGG C^CGG
Ksp632I 556 CTCTTC CTCTTC(1/4)
MspA1I 731 CAGCTG C[AC]G^C[GT]G
MspI 703 CCGG C^CGG
MvaI 425 CCTGG CC^[AT]GG
NlaIV 139 GGTTCC GG[ACGT]^[ACGT]CC
PflMI 56 CCATTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 578 GAGTC GAGTC(4/5)
PstI 80 CTGCAG CTGCA^G
PvuII 731 CAGCTG CAG^CTG
ScaI 746 AGTACT AGT^ACT
ScrFI 425 CCTGG CC^[ACGT]GG
SphI 201 GCATGC GCATG^C
SspI 278 AATATT AAT^ATT
StuI 91 AGGCCT AGG^CCT
StyI 61 CCTTGG C^C[AT][AT]GG
Van91I 56 CCATTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 648 GAAGGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AciI 639 CCGC CCGC(-3/-1)
4 GCGG
AcsI 123 AAATTT [GA]^AATT[CT]
361 GAATTT
AfaI 221 GTAC GT^AC
747 GTAC
ApoI 123 AAATTT [GA]^AATT[CT]
361 GAATTT
Cac8I 93 GCCTGC GC[ACGT]^[ACGT]GC
201 GCATGC
Cfr13I 459 GGGCC G^G[ACGT]CC
615 GGGCC
DpnII 604 GATC ^GATC
717 GATC
HgaI 31 GCGTC GACGC(5/10)
596 GCGTC
MaeIII 193 GTCAC ^GT[ACGT]AC
347 GTGAC
MboI 604 GATC ^GATC
717 GATC
MslI 46 CATGTGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
312 CACCTTTGTG
MwoI 31 GCGTCGGTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
76 GCCACTGCAGC
NdeII 604 GATC ^GATC
717 GATC
RsaI 221 GTAC GT^AC
747 GTAC
Sau3AI 604 GATC ^GATC
717 GATC
Sau96I 459 GGGCC G^G[ACGT]CC
615 GGGCC
TfiI 403 GATTC G^A[AT]TC
706 GATTC
Tsp45I 193 GTCAC ^GT[GC]AC
347 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AluI BbvI BsiYI BslI BsmAI
BsrI DdeI Fnu4HI HaeIII HinfI
HphI ItaI MboII MnlI MseI
NlaIII NspI SfcI Tru9I TseI
Tsp509I TspRI