(Circular) (Six-base) MAPSORT of: pFN21AB7942.seq Check: 8069 from: 1 to: 5440 pFN21AB7942 5440 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2127 2410 2127 Size: 283 5157 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3250 278 Size: 53 83 186 936 1714 2468 Fragments arranged by size: 2468 1714 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2783 1937 Size: 846 4594 Acc65I G'GTAC_C Cuts at: 2761 2761 Size: 5440 AclI AA'CG_TT Cuts at: 1563 2739 3567 3940 1563 Size: 1176 828 373 3063 Fragments arranged by size: 3063 1176 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2711 3503 4887 5155 1632 Size: 1079 792 1384 268 1917 Fragments arranged by size: 1917 1384 1079 792 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5243 AflIII A'CryG_T Cuts at: 1203 2627 4339 5316 1203 Size: 1424 1712 977 1327 Fragments arranged by size: 1712 1424 1327 977 AgeI A'CCGG_T Cuts at: 4271 4271 Size: 5440 AhdI GACnn_n'nnGTC Cuts at: 4169 4169 Size: 5440 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5440 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2336 1184 Size: 1152 4288 AlwNI CAG_nnn'CTG Cuts at: 3183 4755 3183 Size: 1572 3868 ApaLI G'TGCA_C Cuts at: 3497 4653 3497 Size: 1156 4284 ApoI r'AATT_y Cuts at: 1493 2749 2975 3127 5236 1493 Size: 1256 226 152 2109 1697 Fragments arranged by size: 2109 1697 1256 226 152 AseI AT'TA_AT Cuts at: 160 1032 3994 160 Size: 872 2962 1606 Fragments arranged by size: 2962 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5440 AvaI C'yCGr_G Cuts at: 1870 1943 2068 2765 1870 Size: 73 125 697 4545 Fragments arranged by size: 4545 697 125 73 BamHI G'GATC_C Cuts at: 2770 3137 2770 Size: 367 5073 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2725 2761 4216 618 Size: 325 217 627 938 36 1455 1842 Fragments arranged by size: 1842 1455 938 627 325 217 36 BanII G_rGCy'C Cuts at: 1391 2759 1391 Size: 1368 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2385 928 Size: 971 486 3983 Fragments arranged by size: 3983 971 486 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3631 3665 1903 Size: 34 1694 34 3678 Fragments arranged by size: 3678 1694 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3332 4542 3332 Size: 1210 4230 BclI T'GATC_A Cuts at: 1548 1548 Size: 5440 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4051 136 Size: 107 122 71 631 605 2379 1525 Fragments arranged by size: 2379 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5435 5435 Size: 5440 BlpI GC'TnA_GC Cuts at: 2857 2857 Size: 5440 Bme1580I G_kGCm'C Cuts at: 1790 2025 3501 4657 1790 Size: 235 1476 1156 2573 Fragments arranged by size: 2573 1476 1156 235 BmgBI CAC'GTC Cuts at: 1318 2518 1318 Size: 1200 4240 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3171 4129 442 Size: 416 53 83 399 24 1754 958 1753 Fragments arranged by size: 1754 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2477 1056 Size: 1421 4019 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1996 2051 2150 2219 4100 1239 Size: 757 55 99 69 1881 2579 Fragments arranged by size: 2579 1881 757 99 69 55 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3565 4430 4728 3565 Size: 865 298 4277 Fragments arranged by size: 4277 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2554 4103 882 Size: 617 1055 1549 2219 Fragments arranged by size: 2219 1549 1055 617 BsaAI yAC'GTr Cuts at: 493 1204 3231 493 Size: 711 2027 2702 Fragments arranged by size: 2702 2027 711 BsaBI GATnn'nnATC Cuts at: 5434 5434 Size: 5440 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3247 3629 4264 275 Size: 53 83 186 936 1714 382 635 1451 Fragments arranged by size: 1714 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3873 4271 4545 4692 5103 3873 Size: 398 274 147 411 4210 Fragments arranged by size: 4210 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2578 2608 1178 Size: 30 165 30 1175 30 4010 Fragments arranged by size: 4010 1175 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5440 BseYI C'CCAG_C Cuts at: 2047 4643 5139 2047 Size: 2596 496 2348 Fragments arranged by size: 2596 2348 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2438 2631 1163 Size: 573 702 193 3972 Fragments arranged by size: 3972 702 573 193 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2056 2890 3651 4679 1540 Size: 275 186 55 834 761 1028 2301 Fragments arranged by size: 2301 1028 834 761 275 186 55 BsiHKAI G_wGCw'C Cuts at: 3501 3586 4657 3501 Size: 85 1071 4284 Fragments arranged by size: 4284 1071 85 BsmI GAATG_Cn' Cuts at: 2951 3044 2951 Size: 93 5347 BsmBI CGTCTCn'nnnn_ Cuts at: 2508 2598 2508 Size: 90 5350 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2025 2759 3501 3586 4657 1391 Size: 399 235 734 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 734 399 235 85 BspEI T'CCGG_A Cuts at: 5103 5103 Size: 5440 BspHI T'CATG_A Cuts at: 3329 5059 3329 Size: 1730 3710 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2127 2410 2795 844 Size: 1283 283 385 3489 Fragments arranged by size: 3489 1283 385 283 BsrBI CCG'CTC Cuts at: 1394 1823 2035 2347 3327 1394 Size: 429 212 312 980 3507 Fragments arranged by size: 3507 980 429 312 212 BsrDI GCAATG_nn' Cuts at: 65 1248 2673 3935 4109 65 Size: 1183 1425 1262 174 1396 Fragments arranged by size: 1425 1396 1262 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2041 4084 4271 1700 Size: 110 231 2043 187 2869 Fragments arranged by size: 2869 2043 231 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5440 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5440 BssSI C'ACGA_G Cuts at: 3500 4512 3500 Size: 1012 4428 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5440 BstBI TT'CG_AA Cuts at: 4244 4244 Size: 5440 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5440 BstYI r'GATC_y Cuts at: 1972 2770 3137 3522 3539 4991 5077 5089 5435 Size: 798 367 385 17 1452 86 12 346 Cuts at: 5435 1972 Size: 1977 Fragments arranged by size: 1977 1452 798 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2607 513 Size: 552 1542 3346 Fragments arranged by size: 3346 1542 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4701 Fragments arranged by size: 4701 683 56 BtsI GCAGTG_nn' Cuts at: 2956 3750 3770 2956 Size: 794 20 4626 Fragments arranged by size: 4626 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5440 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5405 DraI TTT'AAA Cuts at: 2744 2744 Size: 5440 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4447 809 Size: 507 3131 1802 Fragments arranged by size: 3131 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2053 2185 2664 Size: 54 1425 50 275 18 223 132 479 Cuts at: 2664 2887 3776 5405 8 Size: 223 889 1629 43 Fragments arranged by size: 1629 1425 889 479 275 223 223 132 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2053 2887 1537 Size: 275 241 834 4090 Fragments arranged by size: 4090 834 275 241 EarI CTCTTCn'nnn_ Cuts at: 1353 2270 2434 3370 1353 Size: 917 164 936 3423 Fragments arranged by size: 3423 936 917 164 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2177 4027 4401 4547 387 Size: 1064 138 588 1850 374 146 1280 Fragments arranged by size: 1850 1280 1064 588 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1996 2051 2150 2219 2711 3503 4100 Size: 393 364 55 99 69 492 792 597 Cuts at: 4100 4887 5155 1239 Size: 787 268 1524 Fragments arranged by size: 1524 792 787 597 492 393 364 268 99 69 55 EcoNI CCTnn'n_nnAGG Cuts at: 2405 2405 Size: 5440 EcoRI G'AATT_C Cuts at: 2749 3127 2749 Size: 378 5062 FspI TGC'GCA Cuts at: 3946 3946 Size: 5440 HaeII r_GCGC'y Cuts at: 1678 2048 2607 4587 1678 Size: 370 559 1980 2531 Fragments arranged by size: 2531 1980 559 370 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2341 2373 2561 2578 2593 Size: 32 135 32 769 32 188 17 15 Cuts at: 2593 2610 4081 4113 4155 4187 1373 Size: 17 1471 32 42 32 2626 Fragments arranged by size: 2626 1471 769 188 135 42 32 32 32 32 32 17 17 15 HincII GTy'rAC Cuts at: 669 1657 1938 2737 2784 3030 669 Size: 988 281 799 47 246 3079 Fragments arranged by size: 3079 988 799 281 246 47 HindIII A'AGCT_T Cuts at: 748 748 Size: 5440 HpaI GTT'AAC Cuts at: 3030 3030 Size: 5440 KpnI G_GTAC'C Cuts at: 2765 2765 Size: 5440 MfeI C'AATT_G Cuts at: 3039 3039 Size: 5440 MluI A'CGCG_T Cuts at: 5316 5316 Size: 5440 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4554 4738 1305 Size: 3249 184 2007 Fragments arranged by size: 3249 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2666 10 Size: 54 1425 343 834 2784 Fragments arranged by size: 2784 1425 834 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2660 3398 3757 3916 518 Size: 648 1494 738 359 159 2042 Fragments arranged by size: 2042 1494 738 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2220 2254 2320 2856 3533 4681 4926 1606 Size: 614 34 66 536 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 614 536 245 66 34 NcoI C'CATG_G Cuts at: 513 1065 2607 513 Size: 552 1542 3346 Fragments arranged by size: 3346 1542 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5440 NheI G'CTAG_C Cuts at: 1052 2473 1052 Size: 1421 4019 NotI GC'GGCC_GC Cuts at: 2053 2887 2053 Size: 834 4606 NspI r_CATG'y Cuts at: 1132 1229 2631 2798 4343 1132 Size: 97 1402 167 1545 2229 Fragments arranged by size: 2229 1545 1402 167 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5440 PciI A'CATG_T Cuts at: 2627 4339 2627 Size: 1712 3728 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5440 PmeI GTTT'AAAC Cuts at: 2744 2744 Size: 5440 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3509 3541 5022 5054 3509 Size: 32 1481 32 3895 Fragments arranged by size: 3895 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5440 PsiI TTA'TAA Cuts at: 3010 3010 Size: 5440 PstI C_TGCA'G Cuts at: 830 2091 2325 2792 830 Size: 1261 234 467 3478 Fragments arranged by size: 3478 1261 467 234 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5440 PvuII CAG'CTG Cuts at: 2254 2320 2254 Size: 66 5374 SalI G'TCGA_C Cuts at: 1936 2782 1936 Size: 846 4594 SapI GCTCTTCn'nnn_ Cuts at: 2434 2434 Size: 5440 SbfI CC_TGCA'GG Cuts at: 2091 2792 2091 Size: 701 4739 ScaI AGT'ACT Cuts at: 3688 5400 3688 Size: 1712 3728 SexAI A'CCwGG_T Cuts at: 2721 2721 Size: 5440 SfcI C'TryA_G Cuts at: 826 1045 2087 2321 2788 3923 4604 4795 826 Size: 219 1042 234 467 1135 681 191 1471 Fragments arranged by size: 1471 1135 1042 681 467 234 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5440 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5440 SmaI CCC'GGG Cuts at: 1872 2070 2767 1872 Size: 198 697 4545 Fragments arranged by size: 4545 697 198 SmlI C'TyrA_G Cuts at: 820 1017 1943 3544 4445 4707 820 Size: 197 926 1601 901 262 1553 Fragments arranged by size: 1601 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3231 493 Size: 2738 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5440 SphI G_CATG'C Cuts at: 1132 2798 1132 Size: 1666 3774 SrfI GCCC'GGGC Cuts at: 2070 2070 Size: 5440 SspI AAT'ATT Cuts at: 5 52 3364 5 Size: 47 3312 2081 Fragments arranged by size: 3312 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5440 StyI C'CwwG_G Cuts at: 513 1065 2607 2697 2879 513 Size: 552 1542 90 182 3074 Fragments arranged by size: 3074 1542 552 182 90 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3467 3484 3637 3822 4267 325 Size: 1255 1887 17 153 185 445 1498 Fragments arranged by size: 1887 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3686 5398 96 Size: 274 80 33 51 1443 1709 1712 138 Fragments arranged by size: 1712 1709 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5400 5432 5400 Size: 32 5408 Tth111I GACn'n_nGTC Cuts at: 1316 2590 1316 Size: 1274 4166 XbaI T'CTAG_A Cuts at: 2776 2776 Size: 5440 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5440 XmaI C'CCGG_G Cuts at: 1870 2068 2765 1870 Size: 198 697 4545 Fragments arranged by size: 4545 697 198 XmnI GAAnn'nnTTC Cuts at: 3569 3569 Size: 5440 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3248 276 Size: 53 83 186 936 1714 2468 Fragments arranged by size: 2468 1714 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SwaI XcmI