(Circular) (Six-base) MAPSORT of: pFN21AF0267.seq Check: 9930 from: 1 to: 8008 pFN21AF0267 8008 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2088 2769 4894 2088 Size: 681 2125 5202 Fragments arranged by size: 5202 2125 681 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5818 278 Size: 53 83 186 936 4282 2468 Fragments arranged by size: 4282 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4229 5351 1937 Size: 2292 1122 4594 Fragments arranged by size: 4594 2292 1122 Acc65I G'GTAC_C Cuts at: 4422 5329 4422 Size: 907 7101 AclI AA'CG_TT Cuts at: 1563 3315 6135 6508 1563 Size: 1752 2820 373 3063 Fragments arranged by size: 3063 2820 1752 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3819 4506 4611 5187 6071 7455 7723 1632 Size: 2187 687 105 576 884 1384 268 1917 Fragments arranged by size: 2187 1917 1384 884 687 576 268 105 AfeI AGC'GCT Cuts at: 2381 2381 Size: 8008 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7811 AflIII A'CryG_T Cuts at: 1203 2632 6907 7884 1203 Size: 1429 4275 977 1327 Fragments arranged by size: 4275 1429 1327 977 AgeI A'CCGG_T Cuts at: 6839 6839 Size: 8008 AhdI GACnn_n'nnGTC Cuts at: 3508 6737 3508 Size: 3229 4779 AleI CACnn'nnGTG Cuts at: 1166 2633 1166 Size: 1467 6541 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4912 1184 Size: 3728 4280 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2403 2435 2403 Size: 32 7976 AlwNI CAG_nnn'CTG Cuts at: 3566 3768 3937 4484 5751 7323 3566 Size: 202 169 547 1267 1572 4251 Fragments arranged by size: 4251 1572 1267 547 202 169 ApaI G_GGCC'C Cuts at: 3045 4324 3045 Size: 1279 6729 ApaLI G'TGCA_C Cuts at: 6065 7221 6065 Size: 1156 6852 ApoI r'AATT_y Cuts at: 1493 2129 2210 5317 5543 5695 7804 1493 Size: 636 81 3107 226 152 2109 1697 Fragments arranged by size: 3107 2109 1697 636 226 152 81 AseI AT'TA_AT Cuts at: 160 1032 6562 160 Size: 872 5530 1606 Fragments arranged by size: 5530 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8008 AvaI C'yCGr_G Cuts at: 1870 1943 2709 2726 4900 4950 5016 5333 1870 Size: 73 766 17 2174 50 66 317 4545 Fragments arranged by size: 4545 2174 766 317 73 66 50 17 BamHI G'GATC_C Cuts at: 2311 5186 5338 5705 2311 Size: 2875 152 367 4614 Fragments arranged by size: 4614 2875 367 152 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2101 4422 4822 4882 5329 Size: 325 217 627 314 2321 400 60 447 Cuts at: 5329 6784 618 Size: 1455 1842 Fragments arranged by size: 2321 1842 1455 627 447 400 325 314 217 60 BanII G_rGCy'C Cuts at: 1391 2527 3045 3672 4324 5160 5327 1391 Size: 1136 518 627 652 836 167 4072 Fragments arranged by size: 4072 1136 836 652 627 518 167 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4464 928 Size: 971 2565 4472 Fragments arranged by size: 4472 2565 971 BbvCI CC'TCA_GC Cuts at: 2349 2537 2934 4341 4377 4539 4683 2349 Size: 188 397 1407 36 162 144 5674 Fragments arranged by size: 5674 1407 397 188 162 144 36 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6199 6233 1903 Size: 34 4262 34 3678 Fragments arranged by size: 4262 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5900 7110 5900 Size: 1210 6798 BclI T'GATC_A Cuts at: 1548 1548 Size: 8008 BfrBI ATG'CAT Cuts at: 2497 5267 2497 Size: 2770 5238 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3031 3194 4708 Size: 107 122 71 631 605 1359 163 1514 Cuts at: 4708 4758 4912 6619 136 Size: 50 154 1707 1525 Fragments arranged by size: 1707 1525 1514 1359 631 605 163 154 122 107 71 50 BglII A'GATC_T Cuts at: 8003 8003 Size: 8008 BlpI GC'TnA_GC Cuts at: 3993 5425 3993 Size: 1432 6576 Bme1580I G_kGCm'C Cuts at: 1790 2106 2485 3045 3297 3353 4324 4827 6069 Size: 316 379 560 252 56 971 503 1242 Cuts at: 6069 7225 1790 Size: 1156 2573 Fragments arranged by size: 2573 1242 1156 971 560 503 379 316 252 56 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8008 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3149 4710 4848 Size: 416 53 83 399 24 1732 1561 138 Cuts at: 4848 5739 6697 442 Size: 891 958 1753 Fragments arranged by size: 1753 1732 1561 958 891 416 399 138 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8008 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3718 3718 Size: 8008 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2459 3518 3683 3923 4020 4578 4634 4950 Size: 1220 1059 165 240 97 558 56 316 Cuts at: 4950 5174 6668 1239 Size: 224 1494 2579 Fragments arranged by size: 2579 1494 1220 1059 558 316 240 224 165 97 56 Bpu10I CC'TnA_GC Cuts at: 2349 2376 2537 2934 4341 4377 4539 4683 5144 Size: 27 161 397 1407 36 162 144 461 Cuts at: 5144 2349 Size: 5213 Fragments arranged by size: 5213 1407 461 397 162 161 144 36 27 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3365 3436 3535 5025 6133 6998 7296 3365 Size: 71 99 1490 1108 865 298 4077 Fragments arranged by size: 4077 1490 1108 865 298 99 71 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2185 4037 4539 6671 882 Size: 617 686 1852 502 2132 2219 Fragments arranged by size: 2219 2132 1852 686 617 502 BsaAI yAC'GTr Cuts at: 493 1204 2635 5799 493 Size: 711 1431 3164 2702 Fragments arranged by size: 3164 2702 1431 711 BsaBI GATnn'nnATC Cuts at: 8002 8002 Size: 8008 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5815 6197 6832 275 Size: 53 83 186 936 4282 382 635 1451 Fragments arranged by size: 4282 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6441 6839 7113 7260 7671 6441 Size: 398 274 147 411 6778 Fragments arranged by size: 6778 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2258 2288 2672 2702 3518 Size: 30 165 30 855 30 384 30 816 Cuts at: 3518 3548 4406 4436 1178 Size: 30 858 30 4750 Fragments arranged by size: 4750 858 855 816 384 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2153 2173 2266 3680 3710 3821 4233 4358 Size: 967 20 93 1414 30 111 412 125 Cuts at: 4358 4506 4706 5124 1186 Size: 148 200 418 4070 Fragments arranged by size: 4070 1414 967 418 412 200 148 125 111 93 30 20 BseYI C'CCAG_C Cuts at: 3008 3044 3176 3197 3761 3966 4088 7211 7707 Size: 36 132 21 564 205 122 3123 496 Cuts at: 7707 3008 Size: 3309 Fragments arranged by size: 3309 3123 564 496 205 132 122 36 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2145 3234 3542 5193 1163 Size: 573 409 1089 308 1651 3978 Fragments arranged by size: 3978 1651 1089 573 409 308 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5458 6219 7247 1540 Size: 275 186 3457 761 1028 2301 Fragments arranged by size: 3457 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2079 2838 3672 5160 6069 6154 7225 2079 Size: 759 834 1488 909 85 1071 2862 Fragments arranged by size: 2862 1488 1071 909 834 759 85 BsmI GAATG_Cn' Cuts at: 5519 5612 5519 Size: 93 7915 BsmBI CGTCTCn'nnnn_ Cuts at: 2278 2278 Size: 8008 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2079 2106 2485 2527 2838 3045 3297 Size: 399 289 27 379 42 311 207 252 Cuts at: 3297 3353 3672 4324 4827 5160 5327 6069 6154 Size: 56 319 652 503 333 167 742 85 Cuts at: 6154 7225 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 652 503 399 379 333 319 311 289 252 207 167 85 56 42 27 BspEI T'CCGG_A Cuts at: 7671 7671 Size: 8008 BspHI T'CATG_A Cuts at: 2448 5897 7627 2448 Size: 3449 1730 2829 Fragments arranged by size: 3449 2829 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2088 2244 2541 2769 4894 5247 5363 844 Size: 1244 156 297 228 2125 353 116 3489 Fragments arranged by size: 3489 2125 1244 353 297 228 156 116 BsrBI CCG'CTC Cuts at: 1394 1823 2050 2224 4809 5895 1394 Size: 429 227 174 2585 1086 3507 Fragments arranged by size: 3507 2585 1086 429 227 174 BsrDI GCAATG_nn' Cuts at: 65 1248 3065 4866 6503 6677 65 Size: 1183 1817 1801 1637 174 1396 Fragments arranged by size: 1817 1801 1637 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2328 3366 4911 6652 6839 1700 Size: 110 518 1038 1545 1741 187 2869 Fragments arranged by size: 2869 1741 1545 1038 518 187 110 BsrGI T'GTAC_A Cuts at: 96 4360 96 Size: 4264 3744 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8008 BssSI C'ACGA_G Cuts at: 3171 6068 7080 3171 Size: 2897 1012 4099 Fragments arranged by size: 4099 2897 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8008 BstBI TT'CG_AA Cuts at: 6812 6812 Size: 8008 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8008 BstXI CCAn_nnnn'nTGG Cuts at: 2614 2648 2986 4451 4556 2614 Size: 34 338 1465 105 6066 Fragments arranged by size: 6066 1465 338 105 34 BstYI r'GATC_y Cuts at: 1972 2160 2311 2391 5186 5338 5705 6090 6107 Size: 188 151 80 2795 152 367 385 17 Cuts at: 6107 7559 7645 7657 8003 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2795 1977 1452 385 367 346 188 152 151 86 80 17 12 BstZ17I GTA'TAC Cuts at: 4230 4230 Size: 8008 Bsu36I CC'TnA_GG Cuts at: 2560 3126 3687 4704 2560 Size: 566 561 1017 5864 Fragments arranged by size: 5864 1017 566 561 BtgI C'CryG_G Cuts at: 513 1065 2924 3337 3774 513 Size: 552 1859 413 437 4747 Fragments arranged by size: 4747 1859 552 437 413 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2799 487 Size: 683 56 1573 5696 Fragments arranged by size: 5696 1573 683 56 BtsI GCAGTG_nn' Cuts at: 2266 2747 2966 5524 6318 6338 2266 Size: 481 219 2558 794 20 3936 Fragments arranged by size: 3936 2558 794 481 219 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8008 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7973 DraI TTT'AAA Cuts at: 5312 5312 Size: 8008 DraIII CAC_nnn'GTG Cuts at: 2999 3143 2999 Size: 144 7864 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7015 809 Size: 507 5699 1802 Fragments arranged by size: 5699 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2511 2899 2972 Size: 54 1425 50 275 18 681 388 73 Cuts at: 2972 3056 3324 3368 4909 5455 6344 7973 8 Size: 84 268 44 1541 546 889 1629 43 Fragments arranged by size: 1629 1541 1425 889 681 546 388 275 268 84 73 54 50 44 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5455 1537 Size: 275 3643 4090 Fragments arranged by size: 4090 3643 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3948 4017 4131 4149 4257 4569 4654 4656 Size: 2595 69 114 18 108 312 85 2 Cuts at: 4656 5938 1353 Size: 1282 3423 Fragments arranged by size: 3423 2595 1282 312 114 108 85 69 18 2 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6595 6969 7115 387 Size: 1064 138 5006 374 146 1280 Fragments arranged by size: 5006 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3670 5158 3670 Size: 1488 6520 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2459 3518 3683 3819 3923 4020 4506 Size: 393 827 1059 165 136 104 97 486 Cuts at: 4506 4578 4611 4634 4950 5174 5187 6071 6668 Size: 72 33 23 316 224 13 884 597 Cuts at: 6668 7455 7723 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1059 884 827 787 597 486 393 316 268 224 165 136 104 97 72 33 23 13 EcoNI CCTnn'n_nnAGG Cuts at: 2285 3325 4969 5292 2285 Size: 1040 1644 323 5001 Fragments arranged by size: 5001 1644 1040 323 EcoO109I rG'GnC_Cy Cuts at: 2433 2706 2731 3041 3866 4306 4320 4947 2433 Size: 273 25 310 825 440 14 627 5494 Fragments arranged by size: 5494 825 627 440 310 273 25 14 EcoRI G'AATT_C Cuts at: 5317 5695 5317 Size: 378 7630 FspI TGC'GCA Cuts at: 6514 6514 Size: 8008 HaeII r_GCGC'y Cuts at: 1678 2383 7155 1678 Size: 705 4772 2531 Fragments arranged by size: 4772 2531 705 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2936 2968 3494 3518 3526 Size: 32 135 32 1364 32 526 24 8 Cuts at: 3526 3550 3713 3745 4571 4603 5023 5055 5147 Size: 24 163 32 826 32 420 32 92 Cuts at: 5147 5179 6649 6681 6723 6755 1373 Size: 32 1470 32 42 32 2626 Fragments arranged by size: 2626 1470 1364 826 526 420 163 135 92 42 32 32 32 32 32 32 32 32 32 24 24 8 HincII GTy'rAC Cuts at: 669 1657 1938 3259 4621 5352 5598 669 Size: 988 281 1321 1362 731 246 3079 Fragments arranged by size: 3079 1362 1321 988 731 281 246 HindIII A'AGCT_T Cuts at: 748 2198 748 Size: 1450 6558 HpaI GTT'AAC Cuts at: 5598 5598 Size: 8008 KpnI G_GTAC'C Cuts at: 4426 5333 4426 Size: 907 7101 MfeI C'AATT_G Cuts at: 2366 5607 2366 Size: 3241 4767 MluI A'CGCG_T Cuts at: 7884 7884 Size: 8008 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2211 2979 3414 4822 7122 7306 1305 Size: 906 768 435 1408 2300 184 2007 Fragments arranged by size: 2300 2007 1408 906 768 435 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2513 2901 3326 10 Size: 54 1425 343 681 388 425 4692 Fragments arranged by size: 4692 1425 681 425 388 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2591 2612 2633 5966 6325 6484 518 Size: 648 1425 21 21 3333 359 159 2042 Fragments arranged by size: 3333 2042 1425 648 359 159 21 21 MspA1I CmG'CkG Cuts at: 1606 2579 3008 3765 3785 5424 6101 7249 7494 Size: 973 429 757 20 1639 677 1148 245 Cuts at: 7494 1606 Size: 2120 Fragments arranged by size: 2120 1639 1148 973 757 677 429 245 20 NaeI GCC'GGC Cuts at: 4913 4913 Size: 8008 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7456 NdeI CA'TA_TG Cuts at: 387 5263 387 Size: 4876 3132 NgoMIV G'CCGG_C Cuts at: 4911 4911 Size: 8008 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8008 NotI GC'GGCC_GC Cuts at: 5455 5455 Size: 8008 NsiI A_TGCA'T Cuts at: 2499 5269 2499 Size: 2770 5238 NspI r_CATG'y Cuts at: 1132 1229 2497 2501 3966 4771 5366 6911 1132 Size: 97 1268 4 1465 805 595 1545 2229 Fragments arranged by size: 2229 1545 1465 1268 805 595 97 4 PasI CC'CwG_GG Cuts at: 1348 3632 3647 5288 1348 Size: 2284 15 1641 4068 Fragments arranged by size: 4068 2284 1641 15 PciI A'CATG_T Cuts at: 6907 6907 Size: 8008 PflMI CCAn_nnn'nTGG Cuts at: 1108 2520 3176 1108 Size: 1412 656 5940 Fragments arranged by size: 5940 1412 656 PfoI T'CCnGG_A Cuts at: 4927 5189 4927 Size: 262 7746 PmeI GTTT'AAAC Cuts at: 5312 5312 Size: 8008 PmlI CAC'GTG Cuts at: 2635 2635 Size: 8008 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3293 3325 6077 6109 7590 7622 3293 Size: 32 2752 32 1481 32 3679 Fragments arranged by size: 3679 2752 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2433 2706 2731 3866 4306 4947 2433 Size: 273 25 1135 440 641 5494 Fragments arranged by size: 5494 1135 641 440 273 25 PshAI GACnn'nnGTC Cuts at: 1613 2238 3852 4109 4728 1613 Size: 625 1614 257 619 4893 Fragments arranged by size: 4893 1614 625 619 257 PsiI TTA'TAA Cuts at: 5578 5578 Size: 8008 PspOMI G'GGCC_C Cuts at: 3041 4320 3041 Size: 1279 6729 PstI C_TGCA'G Cuts at: 830 2085 2778 3007 5360 830 Size: 1255 693 229 2353 3478 Fragments arranged by size: 3478 2353 1255 693 229 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8008 PvuII CAG'CTG Cuts at: 3008 3765 3008 Size: 757 7251 SacI G_AGCT'C Cuts at: 3672 5160 3672 Size: 1488 6520 SalI G'TCGA_C Cuts at: 1936 5350 1936 Size: 3414 4594 SanDI GG'GwC_CC Cuts at: 2706 4947 2706 Size: 2241 5767 SapI GCTCTTCn'nnn_ Cuts at: 3948 3948 Size: 8008 SbfI CC_TGCA'GG Cuts at: 5360 5360 Size: 8008 ScaI AGT'ACT Cuts at: 6256 7968 6256 Size: 1712 6296 SfcI C'TryA_G Cuts at: 826 1045 2081 2355 2428 2774 3003 3096 3359 Size: 219 1036 274 73 346 229 93 263 Cuts at: 3359 5356 6491 7172 7363 826 Size: 1997 1135 681 191 1471 Fragments arranged by size: 1997 1471 1135 1036 681 346 274 263 229 219 191 93 73 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8008 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8008 SmaI CCC'GGG Cuts at: 1872 2711 5018 5335 1872 Size: 839 2307 317 4545 Fragments arranged by size: 4545 2307 839 317 SmlI C'TyrA_G Cuts at: 820 1017 1943 3344 3451 3550 5040 6112 7013 Size: 197 926 1401 107 99 1490 1072 901 Cuts at: 7013 7275 820 Size: 262 1553 Fragments arranged by size: 1553 1490 1401 1072 926 901 262 197 107 99 SnaBI TAC'GTA Cuts at: 493 5799 493 Size: 5306 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8008 SphI G_CATG'C Cuts at: 1132 2501 3966 4771 5366 1132 Size: 1369 1465 805 595 3774 Fragments arranged by size: 3774 1465 1369 805 595 SspI AAT'ATT Cuts at: 5 52 5932 5 Size: 47 5880 2081 Fragments arranged by size: 5880 2081 47 StuI AGG'CCT Cuts at: 1517 2291 2558 3796 4906 1517 Size: 774 267 1238 1110 4619 Fragments arranged by size: 4619 1238 1110 774 267 StyI C'CwwG_G Cuts at: 513 1065 3321 3406 4047 4149 4444 5447 513 Size: 552 2256 85 641 102 295 1003 3074 Fragments arranged by size: 3074 2256 1003 641 552 295 102 85 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2049 2676 3131 3562 4745 6035 6052 Size: 1255 469 627 455 431 1183 1290 17 Cuts at: 6052 6205 6390 6835 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1290 1255 1183 627 469 455 445 431 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4360 5066 6254 Size: 274 80 33 51 1443 2383 706 1188 Cuts at: 6254 7966 96 Size: 1712 138 Fragments arranged by size: 2383 1712 1443 1188 706 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2258 2290 2672 2704 7968 8000 2258 Size: 32 382 32 5264 32 2266 Fragments arranged by size: 5264 2266 382 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8008 XbaI T'CTAG_A Cuts at: 5344 5344 Size: 8008 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4295 4295 Size: 8008 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8008 XmaI C'CCGG_G Cuts at: 1870 2709 5016 5333 1870 Size: 839 2307 317 4545 Fragments arranged by size: 4545 2307 839 317 XmnI GAAnn'nnTTC Cuts at: 6137 6137 Size: 8008 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5816 276 Size: 53 83 186 936 4282 2468 Fragments arranged by size: 4282 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BsiWI EcoRV FalI FseI FspAI KasI NarI NruI PacI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI