(Circular) (Six-base) MAPSORT of: pFN21AF0017.seq Check: 6124 from: 1 to: 5887 pFN21AF0017 5887 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2564 2564 Size: 5887 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2283 3697 278 Size: 53 83 186 936 747 1414 2468 Fragments arranged by size: 2468 1414 936 747 186 83 53 AccI GT'mk_AC Cuts at: 1937 3230 1937 Size: 1293 4594 Acc65I G'GTAC_C Cuts at: 2339 3208 2339 Size: 869 5018 AclI AA'CG_TT Cuts at: 1563 4014 4387 1563 Size: 2451 373 3063 Fragments arranged by size: 3063 2451 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2132 3950 5334 5602 1632 Size: 500 1818 1384 268 1917 Fragments arranged by size: 1917 1818 1384 500 268 AfeI AGC'GCT Cuts at: 2639 2639 Size: 5887 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5690 AflIII A'CryG_T Cuts at: 1203 2562 4786 5763 1203 Size: 1359 2224 977 1327 Fragments arranged by size: 2224 1359 1327 977 AgeI A'CCGG_T Cuts at: 4718 4718 Size: 5887 AhdI GACnn_n'nnGTC Cuts at: 4616 4616 Size: 5887 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5887 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5887 AlwNI CAG_nnn'CTG Cuts at: 2092 2110 2446 3630 5202 2092 Size: 18 336 1184 1572 2777 Fragments arranged by size: 2777 1572 1184 336 18 ApaLI G'TGCA_C Cuts at: 3944 5100 3944 Size: 1156 4731 ApoI r'AATT_y Cuts at: 1493 3196 3422 3574 5683 1493 Size: 1703 226 152 2109 1697 Fragments arranged by size: 2109 1703 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4441 160 Size: 872 3409 1606 Fragments arranged by size: 3409 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5887 AvaI C'yCGr_G Cuts at: 1870 1943 2684 3212 1870 Size: 73 741 528 4545 Fragments arranged by size: 4545 741 528 73 BamHI G'GATC_C Cuts at: 2359 3217 3584 2359 Size: 858 367 4662 Fragments arranged by size: 4662 858 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2076 2339 3208 4663 618 Size: 325 217 627 289 263 869 1455 1842 Fragments arranged by size: 1842 1455 869 627 325 289 263 217 BanII G_rGCy'C Cuts at: 1391 2028 2976 3206 1391 Size: 637 948 230 4072 Fragments arranged by size: 4072 948 637 230 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2530 2831 928 Size: 971 631 301 3984 Fragments arranged by size: 3984 971 631 301 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4078 4112 1903 Size: 34 2141 34 3678 Fragments arranged by size: 3678 2141 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2252 3779 4989 2252 Size: 1527 1210 3150 Fragments arranged by size: 3150 1527 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5887 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4498 136 Size: 107 122 71 631 605 2826 1525 Fragments arranged by size: 2826 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5882 5882 Size: 5887 BlpI GC'TnA_GC Cuts at: 2036 3304 2036 Size: 1268 4619 Bme1580I G_kGCm'C Cuts at: 1790 3948 5104 1790 Size: 2158 1156 2573 Fragments arranged by size: 2573 2158 1156 BmgBI CAC'GTC Cuts at: 1318 2829 1318 Size: 1511 4376 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3618 4576 442 Size: 416 53 83 399 24 2201 958 1753 Fragments arranged by size: 2201 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5887 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2749 2944 2749 Size: 195 5692 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2114 2366 2990 4547 1239 Size: 875 252 624 1557 2579 Fragments arranged by size: 2579 1557 875 624 252 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2789 2939 4012 4877 5175 2789 Size: 150 1073 865 298 3501 Fragments arranged by size: 3501 1073 865 298 150 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3157 4550 882 Size: 617 1658 1393 2219 Fragments arranged by size: 2219 1658 1393 617 BsaAI yAC'GTr Cuts at: 493 1204 2563 3678 493 Size: 711 1359 1115 2702 Fragments arranged by size: 2702 1359 1115 711 BsaBI GATnn'nnATC Cuts at: 5881 5881 Size: 5887 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2280 2535 3694 4076 Size: 53 83 186 936 747 255 1159 382 Cuts at: 4076 4711 275 Size: 635 1451 Fragments arranged by size: 1451 1159 936 747 635 382 255 186 83 53 BsaWI w'CCGG_w Cuts at: 2487 4320 4718 4992 5139 5550 2487 Size: 1833 398 274 147 411 2824 Fragments arranged by size: 2824 1833 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5662 Fragments arranged by size: 5662 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2180 1186 Size: 994 4893 BseYI C'CCAG_C Cuts at: 5090 5586 5090 Size: 496 5391 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2954 1163 Size: 573 1218 4096 Fragments arranged by size: 4096 1218 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3337 4098 5126 1540 Size: 275 186 1336 761 1028 2301 Fragments arranged by size: 2301 1336 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2262 2976 3948 4033 5104 2262 Size: 714 972 85 1071 3045 Fragments arranged by size: 3045 1071 972 714 85 BsmI GAATG_Cn' Cuts at: 3398 3491 3398 Size: 93 5794 BsmBI CGTCTCn'nnnn_ Cuts at: 2042 2042 Size: 5887 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2028 2262 2976 3206 3948 4033 5104 Size: 399 238 234 714 230 742 85 1071 Cuts at: 5104 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 714 399 238 234 230 85 BspEI T'CCGG_A Cuts at: 5550 5550 Size: 5887 BspHI T'CATG_A Cuts at: 3776 5506 3776 Size: 1730 4157 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2365 2379 2564 2688 3175 3242 844 Size: 1521 14 185 124 487 67 3489 Fragments arranged by size: 3489 1521 487 185 124 67 14 BsrBI CCG'CTC Cuts at: 1394 1823 3774 1394 Size: 429 1951 3507 Fragments arranged by size: 3507 1951 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2289 2761 4382 4556 65 Size: 1183 1041 472 1621 174 1396 Fragments arranged by size: 1621 1396 1183 1041 472 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4531 4718 1700 Size: 110 2721 187 2869 Fragments arranged by size: 2869 2721 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5887 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5887 BssSI C'ACGA_G Cuts at: 2402 3947 4959 2402 Size: 1545 1012 3330 Fragments arranged by size: 3330 1545 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2266 794 Size: 1472 4415 BstBI TT'CG_AA Cuts at: 4691 4691 Size: 5887 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5887 BstYI r'GATC_y Cuts at: 1972 2359 3217 3584 3969 3986 5438 5524 5536 Size: 387 858 367 385 17 1452 86 12 Cuts at: 5536 5882 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 858 387 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2435 2742 2435 Size: 307 5580 BtgI C'CryG_G Cuts at: 513 1065 2004 2028 2656 2900 513 Size: 552 939 24 628 244 3500 Fragments arranged by size: 3500 939 628 552 244 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5148 Fragments arranged by size: 5148 683 56 BtsI GCAGTG_nn' Cuts at: 3403 4197 4217 3403 Size: 794 20 5073 Fragments arranged by size: 5073 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5887 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5852 DraI TTT'AAA Cuts at: 3191 3191 Size: 5887 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4894 809 Size: 507 3578 1802 Fragments arranged by size: 3578 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3334 4223 5852 Size: 54 1425 50 275 18 1504 889 1629 Cuts at: 5852 8 Size: 43 Fragments arranged by size: 1629 1504 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3334 1537 Size: 275 1522 4090 Fragments arranged by size: 4090 1522 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2049 2711 3817 1353 Size: 696 662 1106 3423 Fragments arranged by size: 3423 1106 696 662 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4474 4848 4994 387 Size: 1064 138 2885 374 146 1280 Fragments arranged by size: 2885 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2974 2974 Size: 5887 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2114 2132 2366 2990 3950 4547 5334 Size: 393 482 18 234 624 960 597 787 Cuts at: 5334 5602 1239 Size: 268 1524 Fragments arranged by size: 1524 960 787 624 597 482 393 268 234 18 EcoNI CCTnn'n_nnAGG Cuts at: 2426 2684 2426 Size: 258 5629 EcoO109I rG'GnC_Cy Cuts at: 2317 2377 2431 2317 Size: 60 54 5773 Fragments arranged by size: 5773 60 54 EcoRI G'AATT_C Cuts at: 3196 3574 3196 Size: 378 5509 FspI TGC'GCA Cuts at: 4393 4393 Size: 5887 HaeII r_GCGC'y Cuts at: 1678 2641 2842 5034 1678 Size: 963 201 2192 2531 Fragments arranged by size: 2531 2192 963 201 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2505 2537 3108 3137 3140 Size: 32 135 32 933 32 571 29 3 Cuts at: 3140 3169 4528 4560 4602 4634 1373 Size: 29 1359 32 42 32 2626 Fragments arranged by size: 2626 1359 933 571 135 42 32 32 32 32 32 29 29 3 HincII GTy'rAC Cuts at: 669 1657 1938 3231 3477 669 Size: 988 281 1293 246 3079 Fragments arranged by size: 3079 1293 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5887 HpaI GTT'AAC Cuts at: 3477 3477 Size: 5887 KpnI G_GTAC'C Cuts at: 2343 3212 2343 Size: 869 5018 MfeI C'AATT_G Cuts at: 3486 3486 Size: 5887 MluI A'CGCG_T Cuts at: 5763 5763 Size: 5887 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5001 5185 1305 Size: 3696 184 2007 Fragments arranged by size: 3696 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4065 Fragments arranged by size: 4065 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2582 3845 4204 4363 518 Size: 648 1416 1263 359 159 2042 Fragments arranged by size: 2042 1416 1263 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2030 2043 2658 2695 3303 3980 5128 5373 Size: 424 13 615 37 608 677 1148 245 Cuts at: 5373 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 615 608 424 245 37 13 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4396 Fragments arranged by size: 4396 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5887 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5887 NotI GC'GGCC_GC Cuts at: 3334 3334 Size: 5887 NspI r_CATG'y Cuts at: 1132 1229 3245 4790 1132 Size: 97 2016 1545 2229 Fragments arranged by size: 2229 2016 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5887 PciI A'CATG_T Cuts at: 4786 4786 Size: 5887 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5887 PfoI T'CCnGG_A Cuts at: 2091 2283 2616 2091 Size: 192 333 5362 Fragments arranged by size: 5362 333 192 PmeI GTTT'AAAC Cuts at: 3191 3191 Size: 5887 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3956 3988 5469 5501 3956 Size: 32 1481 32 4342 Fragments arranged by size: 4342 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2377 2377 Size: 5887 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5887 PsiI TTA'TAA Cuts at: 3457 3457 Size: 5887 PstI C_TGCA'G Cuts at: 830 2376 3239 830 Size: 1546 863 3478 Fragments arranged by size: 3478 1546 863 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5887 PvuII CAG'CTG Cuts at: 2695 2695 Size: 5887 SacI G_AGCT'C Cuts at: 2976 2976 Size: 5887 SacII CC_GC'GG Cuts at: 2031 2659 2031 Size: 628 5259 SalI G'TCGA_C Cuts at: 1936 3229 1936 Size: 1293 4594 SbfI CC_TGCA'GG Cuts at: 2376 3239 2376 Size: 863 5024 ScaI AGT'ACT Cuts at: 4135 5847 4135 Size: 1712 4175 SexAI A'CCwGG_T Cuts at: 3147 3147 Size: 5887 SfcI C'TryA_G Cuts at: 826 1045 2372 3235 4370 5051 5242 826 Size: 219 1327 863 1135 681 191 1471 Fragments arranged by size: 1471 1327 1135 863 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5887 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5887 SmaI CCC'GGG Cuts at: 1872 3214 1872 Size: 1342 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2804 2918 3991 4892 5154 820 Size: 197 926 861 114 1073 901 262 1553 Fragments arranged by size: 1553 1073 926 901 861 262 197 114 SnaBI TAC'GTA Cuts at: 493 3678 493 Size: 3185 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5887 SphI G_CATG'C Cuts at: 1132 3245 1132 Size: 2113 3774 SspI AAT'ATT Cuts at: 5 52 3811 5 Size: 47 3759 2081 Fragments arranged by size: 3759 2081 47 StuI AGG'CCT Cuts at: 1517 2387 2501 3011 1517 Size: 870 114 510 4393 Fragments arranged by size: 4393 870 510 114 StyI C'CwwG_G Cuts at: 513 1065 2004 2235 3326 513 Size: 552 939 231 1091 3074 Fragments arranged by size: 3074 1091 939 552 231 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2034 2870 3035 3914 3931 4084 4269 Size: 1255 454 836 165 879 17 153 185 Cuts at: 4269 4714 325 Size: 445 1498 Fragments arranged by size: 1498 1255 879 836 454 445 185 165 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4133 5845 96 Size: 274 80 33 51 1443 2156 1712 138 Fragments arranged by size: 2156 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5847 5879 5847 Size: 32 5855 Tth111I GACn'n_nGTC Cuts at: 1316 3149 1316 Size: 1833 4054 XbaI T'CTAG_A Cuts at: 3223 3223 Size: 5887 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5887 XmaI C'CCGG_G Cuts at: 1870 3212 1870 Size: 1342 4545 XmnI GAAnn'nnTTC Cuts at: 2705 2960 4016 2705 Size: 255 1056 4576 Fragments arranged by size: 4576 1056 255 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2281 3695 276 Size: 53 83 186 936 747 1414 2468 Fragments arranged by size: 2468 1414 936 747 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SanDI SapI SfiI SfoI SrfI SwaI XcmI