(Circular) (Six-base) MAPSORT of: pFN21AF0006.seq Check: 5952 from: 1 to: 6952 pFN21AF0006 6952 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2032 3988 2032 Size: 1956 4996 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4762 278 Size: 53 83 186 936 3226 2468 Fragments arranged by size: 3226 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4295 1937 Size: 2358 4594 Acc65I G'GTAC_C Cuts at: 4056 4273 4056 Size: 217 6735 AclI AA'CG_TT Cuts at: 1563 5079 5452 1563 Size: 3516 373 3063 Fragments arranged by size: 3516 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2698 3752 3838 4120 4211 4216 5015 6399 Size: 1066 1054 86 282 91 5 799 1384 Cuts at: 6399 6667 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1066 1054 799 282 268 91 86 5 AflII C'TTAA_G Cuts at: 820 1017 3707 820 Size: 197 2690 4065 Fragments arranged by size: 4065 2690 197 AflIII A'CryG_T Cuts at: 1203 5851 6828 1203 Size: 4648 977 1327 Fragments arranged by size: 4648 1327 977 AgeI A'CCGG_T Cuts at: 5783 5783 Size: 6952 AhdI GACnn_n'nnGTC Cuts at: 5681 5681 Size: 6952 AleI CACnn'nnGTG Cuts at: 1166 2371 3092 3530 1166 Size: 1205 721 438 4588 Fragments arranged by size: 4588 1205 721 438 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2561 2741 1184 Size: 1377 180 5395 Fragments arranged by size: 5395 1377 180 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2981 3013 2981 Size: 32 6920 AlwNI CAG_nnn'CTG Cuts at: 2227 2779 3157 4003 4189 4695 6267 2227 Size: 552 378 846 186 506 1572 2912 Fragments arranged by size: 2912 1572 846 552 506 378 186 ApaI G_GGCC'C Cuts at: 2395 2575 2395 Size: 180 6772 ApaLI G'TGCA_C Cuts at: 3346 5009 6165 3346 Size: 1663 1156 4133 Fragments arranged by size: 4133 1663 1156 ApoI r'AATT_y Cuts at: 1493 4261 4487 4639 6748 1493 Size: 2768 226 152 2109 1697 Fragments arranged by size: 2768 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5506 160 Size: 872 4474 1606 Fragments arranged by size: 4474 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6952 AvaI C'yCGr_G Cuts at: 1870 1943 2922 4084 4277 1870 Size: 73 979 1162 193 4545 Fragments arranged by size: 4545 1162 979 193 73 BamHI G'GATC_C Cuts at: 4282 4649 4282 Size: 367 6585 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2545 2612 3167 3450 3755 Size: 325 217 627 758 67 555 283 305 Cuts at: 3755 4056 4273 5728 618 Size: 301 217 1455 1842 Fragments arranged by size: 1842 1455 758 627 555 325 305 301 283 217 217 67 BanII G_rGCy'C Cuts at: 1391 2395 2575 2921 3570 3990 4113 4161 4271 Size: 1004 180 346 649 420 123 48 110 Cuts at: 4271 1391 Size: 4072 Fragments arranged by size: 4072 1004 649 420 346 180 123 110 48 BbeI G_GCGC'C Cuts at: 3454 3759 3454 Size: 305 6647 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5981 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3770 3804 4104 4138 5143 5177 1903 Size: 34 1833 34 300 34 1005 34 3678 Fragments arranged by size: 3678 1833 1005 300 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4844 6054 4844 Size: 1210 5742 BclI T'GATC_A Cuts at: 1548 2829 1548 Size: 1281 5671 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3582 3710 5563 Size: 107 122 71 631 605 1910 128 1853 Cuts at: 5563 136 Size: 1525 Fragments arranged by size: 1910 1853 1525 631 605 128 122 107 71 BglII A'GATC_T Cuts at: 2073 6947 2073 Size: 4874 2078 BlpI GC'TnA_GC Cuts at: 4369 4369 Size: 6952 Bme1580I G_kGCm'C Cuts at: 1790 2395 2550 2575 2615 3350 5013 6169 1790 Size: 605 155 25 40 735 1663 1156 2573 Fragments arranged by size: 2573 1663 1156 735 605 155 40 25 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6952 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4162 4683 5641 Size: 416 53 83 399 24 2745 521 958 Cuts at: 5641 442 Size: 1753 Fragments arranged by size: 2745 1753 958 521 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6952 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2194 3710 2194 Size: 1516 5436 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2164 2267 2669 2786 3278 3366 4015 4037 Size: 925 103 402 117 492 88 649 22 Cuts at: 4037 5612 1239 Size: 1575 2579 Fragments arranged by size: 2579 1575 925 649 492 402 117 103 88 22 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2945 3251 5077 5942 6240 2945 Size: 306 1826 865 298 3657 Fragments arranged by size: 3657 1826 865 306 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2453 5615 882 Size: 617 954 3162 2219 Fragments arranged by size: 3162 2219 954 617 BsaAI yAC'GTr Cuts at: 493 1204 3427 4743 493 Size: 711 2223 1316 2702 Fragments arranged by size: 2702 2223 1316 711 BsaBI GATnn'nnATC Cuts at: 6946 6946 Size: 6952 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2661 3451 3756 4200 Size: 53 83 186 936 1128 790 305 444 Cuts at: 4200 4759 5141 5776 275 Size: 559 382 635 1451 Fragments arranged by size: 1451 1128 936 790 635 559 444 382 305 186 83 53 BsaWI w'CCGG_w Cuts at: 5385 5783 6057 6204 6615 5385 Size: 398 274 147 411 5722 Fragments arranged by size: 5722 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2903 2933 2981 3011 3803 Size: 30 165 30 1500 30 48 30 792 Cuts at: 3803 3833 4040 4070 1178 Size: 30 207 30 4060 Fragments arranged by size: 4060 1500 792 207 165 48 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2666 2669 3797 3905 1186 Size: 1480 3 1128 108 4233 Fragments arranged by size: 4233 1480 1128 108 3 BseYI C'CCAG_C Cuts at: 2507 2728 2944 3207 3499 3555 4089 4236 6155 Size: 221 216 263 292 56 534 147 1919 Cuts at: 6155 6651 2507 Size: 496 2808 Fragments arranged by size: 2808 1919 534 496 292 263 221 216 147 56 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2822 2939 3932 3989 1163 Size: 573 1086 117 993 57 4126 Fragments arranged by size: 4126 1086 993 573 117 57 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4402 5163 6191 1540 Size: 275 186 2401 761 1028 2301 Fragments arranged by size: 2401 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2568 3350 3437 3552 3570 3843 4026 4113 4161 Size: 782 87 115 18 273 183 87 48 Cuts at: 4161 5013 5098 6169 2568 Size: 852 85 1071 3351 Fragments arranged by size: 3351 1071 852 782 273 183 115 87 87 85 48 18 BsmI GAATG_Cn' Cuts at: 4463 4556 4463 Size: 93 6859 BsmBI CGTCTCn'nnnn_ Cuts at: 2305 2305 Size: 6952 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2395 2550 2568 2575 2615 2921 3350 Size: 399 605 155 18 7 40 306 429 Cuts at: 3350 3437 3552 3570 3843 3990 4026 4113 4161 Size: 87 115 18 273 147 36 87 48 Cuts at: 4161 4271 5013 5098 6169 1391 Size: 110 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 605 429 399 306 273 155 147 115 110 87 87 85 48 40 36 18 18 7 BspEI T'CCGG_A Cuts at: 6615 6615 Size: 6952 BspHI T'CATG_A Cuts at: 4841 6571 4841 Size: 1730 5222 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2032 2499 2703 2787 3988 4307 844 Size: 1188 467 204 84 1201 319 3489 Fragments arranged by size: 3489 1201 1188 467 319 204 84 BsrBI CCG'CTC Cuts at: 1394 1823 2313 2328 3481 3969 4839 1394 Size: 429 490 15 1153 488 870 3507 Fragments arranged by size: 3507 1153 870 490 488 429 15 BsrDI GCAATG_nn' Cuts at: 65 1248 3135 5447 5621 65 Size: 1183 1887 2312 174 1396 Fragments arranged by size: 2312 1887 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2342 2615 3077 3164 3447 5596 5783 Size: 110 532 273 462 87 283 2149 187 Cuts at: 5783 1700 Size: 2869 Fragments arranged by size: 2869 2149 532 462 283 273 187 110 87 BsrGI T'GTAC_A Cuts at: 96 2151 96 Size: 2055 4897 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6952 BssSI C'ACGA_G Cuts at: 3416 3680 5012 6024 3416 Size: 264 1332 1012 4344 Fragments arranged by size: 4344 1332 1012 264 BstAPI GCAn_nnn'nTGC Cuts at: 794 2517 3944 4003 794 Size: 1723 1427 59 3743 Fragments arranged by size: 3743 1723 1427 59 BstBI TT'CG_AA Cuts at: 3720 5756 3720 Size: 2036 4916 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6952 BstXI CCAn_nnnn'nTGG Cuts at: 2188 2373 2728 2869 3073 3604 2188 Size: 185 355 141 204 531 5536 Fragments arranged by size: 5536 531 355 204 185 141 BstYI r'GATC_y Cuts at: 1972 2073 2275 4282 4649 5034 5051 6503 6589 Size: 101 202 2007 367 385 17 1452 86 Cuts at: 6589 6601 6947 1972 Size: 12 346 1977 Fragments arranged by size: 2007 1977 1452 385 367 346 202 101 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3099 4044 513 Size: 552 2034 945 3421 Fragments arranged by size: 3421 2034 945 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2449 3625 487 Size: 683 56 1223 1176 3814 Fragments arranged by size: 3814 1223 1176 683 56 BtsI GCAGTG_nn' Cuts at: 2834 3228 3550 3945 4468 5262 5282 2834 Size: 394 322 395 523 794 20 4504 Fragments arranged by size: 4504 794 523 395 394 322 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6952 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6917 DraI TTT'AAA Cuts at: 4256 4256 Size: 6952 DraIII CAC_nnn'GTG Cuts at: 2269 2269 Size: 6952 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2750 5959 809 Size: 507 1434 3209 1802 Fragments arranged by size: 3209 1802 1434 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2079 2340 2625 Size: 54 1425 50 275 18 249 261 285 Cuts at: 2625 3063 3075 3096 4399 5288 6917 8 Size: 438 12 21 1303 889 1629 43 Fragments arranged by size: 1629 1425 1303 889 438 285 275 261 249 54 50 43 21 18 12 EagI C'GGCC_G Cuts at: 1537 1812 4399 1537 Size: 275 2587 4090 Fragments arranged by size: 4090 2587 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2094 2399 2964 3245 4163 4235 4882 1353 Size: 741 305 565 281 918 72 647 3423 Fragments arranged by size: 3423 918 741 647 565 305 281 72 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5539 5913 6059 387 Size: 1064 138 3950 374 146 1280 Fragments arranged by size: 3950 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3568 4111 4159 3568 Size: 543 48 6361 Fragments arranged by size: 6361 543 48 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2164 2267 2669 2698 2786 3278 3366 Size: 393 532 103 402 29 88 492 88 Cuts at: 3366 3752 3838 4015 4037 4120 4211 4216 5015 Size: 386 86 177 22 83 91 5 799 Cuts at: 5015 5612 6399 6667 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 799 787 597 532 492 402 393 386 268 177 103 91 88 88 86 83 29 22 5 EcoNI CCTnn'n_nnAGG Cuts at: 2651 2963 3185 4145 4193 2651 Size: 312 222 960 48 5410 Fragments arranged by size: 5410 960 312 222 48 EcoO109I rG'GnC_Cy Cuts at: 2391 2392 2488 2572 2692 2752 2899 3204 3463 Size: 1 96 84 120 60 147 305 259 Cuts at: 3463 3690 3712 2391 Size: 227 22 5631 Fragments arranged by size: 5631 305 259 227 147 120 96 84 60 22 1 EcoRI G'AATT_C Cuts at: 4261 4639 4261 Size: 378 6574 EcoRV GAT'ATC Cuts at: 3022 3022 Size: 6952 FspI TGC'GCA Cuts at: 2558 2891 4079 5458 2558 Size: 333 1188 1379 4052 Fragments arranged by size: 4052 1379 1188 333 HaeII r_GCGC'y Cuts at: 1678 3115 3454 3759 6099 1678 Size: 1437 339 305 2340 2531 Fragments arranged by size: 2531 2340 1437 339 305 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3011 3043 3803 3835 5593 Size: 32 135 32 1439 32 760 32 1758 Cuts at: 5593 5625 5667 5699 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1758 1439 760 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4296 4542 669 Size: 988 281 2358 246 3079 Fragments arranged by size: 3079 2358 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6952 HpaI GTT'AAC Cuts at: 4542 4542 Size: 6952 KasI G'GCGC_C Cuts at: 3450 3755 3450 Size: 305 6647 KpnI G_GTAC'C Cuts at: 4060 4277 4060 Size: 217 6735 MfeI C'AATT_G Cuts at: 4551 4551 Size: 6952 MluI A'CGCG_T Cuts at: 6828 6828 Size: 6952 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2475 3840 6066 6250 1305 Size: 1170 1365 2226 184 2007 Fragments arranged by size: 2226 2007 1365 1170 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2081 3065 10 Size: 54 1425 343 249 984 3897 Fragments arranged by size: 3897 1425 984 343 249 54 MslI CAynn'nnrTG Cuts at: 518 1166 2371 2543 2618 3092 3530 4214 4910 Size: 648 1205 172 75 474 438 684 696 Cuts at: 4910 5269 5428 518 Size: 359 159 2042 Fragments arranged by size: 2042 1205 696 684 648 474 438 359 172 159 75 MspA1I CmG'CkG Cuts at: 1606 2371 2749 3211 3409 4236 4368 5045 6193 Size: 765 378 462 198 827 132 677 1148 Cuts at: 6193 6438 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 827 765 677 462 378 245 198 132 NaeI GCC'GGC Cuts at: 2344 3079 3166 2344 Size: 735 87 6130 Fragments arranged by size: 6130 735 87 NarI GG'CG_CC Cuts at: 3451 3756 3451 Size: 305 6647 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6400 NdeI CA'TA_TG Cuts at: 387 2060 387 Size: 1673 5279 NgoMIV G'CCGG_C Cuts at: 2342 3077 3164 2342 Size: 735 87 6130 Fragments arranged by size: 6130 735 87 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6952 NotI GC'GGCC_GC Cuts at: 4399 4399 Size: 6952 NspI r_CATG'y Cuts at: 1132 1229 2953 3947 4213 4310 5855 1132 Size: 97 1724 994 266 97 1545 2229 Fragments arranged by size: 2229 1724 1545 994 266 97 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6952 PciI A'CATG_T Cuts at: 5851 5851 Size: 6952 PflMI CCAn_nnn'nTGG Cuts at: 1108 2269 3157 1108 Size: 1161 888 4903 Fragments arranged by size: 4903 1161 888 PfoI T'CCnGG_A Cuts at: 2646 2802 3309 3612 4203 2646 Size: 156 507 303 591 5395 Fragments arranged by size: 5395 591 507 303 156 PmeI GTTT'AAAC Cuts at: 4256 4256 Size: 6952 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2981 3013 5021 5053 6534 6566 2981 Size: 32 2008 32 1481 32 3367 Fragments arranged by size: 3367 2008 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2488 2692 2752 2899 3204 3690 2488 Size: 204 60 147 305 486 5750 Fragments arranged by size: 5750 486 305 204 147 60 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6952 PsiI TTA'TAA Cuts at: 4522 4522 Size: 6952 PspOMI G'GGCC_C Cuts at: 2391 2571 2391 Size: 180 6772 PstI C_TGCA'G Cuts at: 830 2400 3368 3793 4304 830 Size: 1570 968 425 511 3478 Fragments arranged by size: 3478 1570 968 511 425 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6952 PvuII CAG'CTG Cuts at: 2371 4236 2371 Size: 1865 5087 SacI G_AGCT'C Cuts at: 3570 4113 4161 3570 Size: 543 48 6361 Fragments arranged by size: 6361 543 48 SalI G'TCGA_C Cuts at: 1936 4294 1936 Size: 2358 4594 SanDI GG'GwC_CC Cuts at: 3690 3690 Size: 6952 SapI GCTCTTCn'nnn_ Cuts at: 2964 3245 2964 Size: 281 6671 SbfI CC_TGCA'GG Cuts at: 4304 4304 Size: 6952 ScaI AGT'ACT Cuts at: 3635 5200 6912 3635 Size: 1565 1712 3675 Fragments arranged by size: 3675 1712 1565 SfcI C'TryA_G Cuts at: 826 1045 2396 3364 3388 3789 4300 5435 6116 Size: 219 1351 968 24 401 511 1135 681 Cuts at: 6116 6307 826 Size: 191 1471 Fragments arranged by size: 1471 1351 1135 968 681 511 401 219 191 24 SfiI GGCCn_nnn'nGGCC Cuts at: 3710 3710 Size: 6952 SfoI GGC'GCC Cuts at: 3452 3757 3452 Size: 305 6647 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6952 SgrAI Cr'CCGG_yG Cuts at: 1700 3447 1700 Size: 1747 5205 SmaI CCC'GGG Cuts at: 1872 2924 4086 4279 1872 Size: 1052 1162 193 4545 Fragments arranged by size: 4545 1162 1052 193 SmlI C'TyrA_G Cuts at: 820 1017 1943 2960 3266 3707 5056 5957 6219 Size: 197 926 1017 306 441 1349 901 262 Cuts at: 6219 820 Size: 1553 Fragments arranged by size: 1553 1349 1017 926 901 441 306 262 197 SnaBI TAC'GTA Cuts at: 493 4743 493 Size: 4250 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6952 SphI G_CATG'C Cuts at: 1132 2953 3947 4310 1132 Size: 1821 994 363 3774 Fragments arranged by size: 3774 1821 994 363 SrfI GCCC'GGGC Cuts at: 4086 4086 Size: 6952 SspI AAT'ATT Cuts at: 5 52 4876 5 Size: 47 4824 2081 Fragments arranged by size: 4824 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6952 StyI C'CwwG_G Cuts at: 513 1065 3459 4391 513 Size: 552 2394 932 3074 Fragments arranged by size: 3074 2394 932 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2270 2720 3547 4979 4996 5149 5334 Size: 1255 690 450 827 1432 17 153 185 Cuts at: 5334 5779 325 Size: 445 1498 Fragments arranged by size: 1498 1432 1255 827 690 450 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2085 2151 3633 Size: 274 80 33 51 1443 108 66 1482 Cuts at: 3633 5198 6910 96 Size: 1565 1712 138 Fragments arranged by size: 1712 1565 1482 1443 274 138 108 80 66 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3324 3356 6912 6944 3324 Size: 32 3556 32 3332 Fragments arranged by size: 3556 3332 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6952 XbaI T'CTAG_A Cuts at: 4288 4288 Size: 6952 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3325 3466 3325 Size: 141 6811 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6952 XmaI C'CCGG_G Cuts at: 1870 2922 4084 4277 1870 Size: 1052 1162 193 4545 Fragments arranged by size: 4545 1162 1052 193 XmnI GAAnn'nnTTC Cuts at: 5081 5081 Size: 6952 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4760 276 Size: 53 83 186 936 3226 2468 Fragments arranged by size: 3226 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SexAI SwaI