(Circular) (Six-base) MAPSORT of: pFN21AB9885.seq Check: 1582 from: 1 to: 6664 pFN21AB9885 6664 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3031 3031 Size: 6664 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4474 278 Size: 53 83 186 936 2938 2468 Fragments arranged by size: 2938 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3187 3585 4007 1937 Size: 1250 398 422 4594 Fragments arranged by size: 4594 1250 422 398 Acc65I G'GTAC_C Cuts at: 3985 3985 Size: 6664 AclI AA'CG_TT Cuts at: 1563 4791 5164 1563 Size: 3228 373 3063 Fragments arranged by size: 3228 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2519 2658 4727 6111 6379 1632 Size: 887 139 2069 1384 268 1917 Fragments arranged by size: 2069 1917 1384 887 268 139 AflII C'TTAA_G Cuts at: 820 1017 2846 820 Size: 197 1829 4638 Fragments arranged by size: 4638 1829 197 AflIII A'CryG_T Cuts at: 1203 5563 6540 1203 Size: 4360 977 1327 Fragments arranged by size: 4360 1327 977 AgeI A'CCGG_T Cuts at: 5495 5495 Size: 6664 AhdI GACnn_n'nnGTC Cuts at: 5393 5393 Size: 6664 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6664 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3331 1184 Size: 2147 4517 AlwNI CAG_nnn'CTG Cuts at: 2146 2945 3325 3895 4407 5979 2146 Size: 799 380 570 512 1572 2831 Fragments arranged by size: 2831 1572 799 570 512 380 ApaLI G'TGCA_C Cuts at: 4721 5877 4721 Size: 1156 5508 ApoI r'AATT_y Cuts at: 1493 3554 3798 3973 4199 4351 6460 1493 Size: 2061 244 175 226 152 2109 1697 Fragments arranged by size: 2109 2061 1697 244 226 175 152 AseI AT'TA_AT Cuts at: 160 1032 5218 160 Size: 872 4186 1606 Fragments arranged by size: 4186 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6664 AvaI C'yCGr_G Cuts at: 1870 1943 2814 3989 1870 Size: 73 871 1175 4545 Fragments arranged by size: 4545 1175 871 73 AvrII C'CTAG_G Cuts at: 3011 3011 Size: 6664 BamHI G'GATC_C Cuts at: 3994 4361 3994 Size: 367 6297 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3578 3985 5440 618 Size: 325 217 627 1791 407 1455 1842 Fragments arranged by size: 1842 1791 1455 627 407 325 217 BanII G_rGCy'C Cuts at: 1391 2277 2370 2799 2823 2844 2990 3983 1391 Size: 886 93 429 24 21 146 993 4072 Fragments arranged by size: 4072 993 886 429 146 93 24 21 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5693 BbvCI CC'TCA_GC Cuts at: 2430 3573 2430 Size: 1143 5521 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3671 3705 4855 4889 1903 Size: 34 1734 34 1150 34 3678 Fragments arranged by size: 3678 1734 1150 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4556 5766 4556 Size: 1210 5454 BclI T'GATC_A Cuts at: 1548 1548 Size: 6664 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2933 3240 5275 Size: 107 122 71 631 605 1261 307 2035 Cuts at: 5275 136 Size: 1525 Fragments arranged by size: 2035 1525 1261 631 605 307 122 107 71 BglII A'GATC_T Cuts at: 3277 3927 6659 3277 Size: 650 2732 3282 Fragments arranged by size: 3282 2732 650 BlpI GC'TnA_GC Cuts at: 2276 3260 3410 4081 2276 Size: 984 150 671 4859 Fragments arranged by size: 4859 984 671 150 Bme1580I G_kGCm'C Cuts at: 1790 3581 4725 5881 1790 Size: 1791 1144 1156 2573 Fragments arranged by size: 2573 1791 1156 1144 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6664 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3231 3956 4395 Size: 416 53 83 399 24 1814 725 439 Cuts at: 4395 5353 442 Size: 958 1753 Fragments arranged by size: 1814 1753 958 725 439 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6664 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3067 3067 Size: 6664 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2168 2543 2786 3090 3635 3662 5324 1239 Size: 929 375 243 304 545 27 1662 2579 Fragments arranged by size: 2579 1662 929 545 375 304 243 27 Bpu10I CC'TnA_GC Cuts at: 2430 3573 3601 2430 Size: 1143 28 5493 Fragments arranged by size: 5493 1143 28 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3062 3344 4789 5654 5952 3062 Size: 282 1445 865 298 3774 Fragments arranged by size: 3774 1445 865 298 282 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5327 882 Size: 617 3828 2219 Fragments arranged by size: 3828 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3139 4455 493 Size: 711 1935 1316 2702 Fragments arranged by size: 2702 1935 1316 711 BsaBI GATnn'nnATC Cuts at: 6658 6658 Size: 6664 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2320 2440 4471 4853 Size: 53 83 186 936 787 120 2031 382 Cuts at: 4853 5488 275 Size: 635 1451 Fragments arranged by size: 2031 1451 936 787 635 382 186 120 83 53 BsaWI w'CCGG_w Cuts at: 3859 5097 5495 5769 5916 6327 3859 Size: 1238 398 274 147 411 4196 Fragments arranged by size: 4196 1238 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2335 2365 1178 Size: 30 165 30 932 30 5477 Fragments arranged by size: 5477 932 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2447 2591 2684 2783 2798 2801 3285 1186 Size: 1261 144 93 99 15 3 484 4565 Fragments arranged by size: 4565 1261 484 144 99 93 15 3 BseYI C'CCAG_C Cuts at: 2226 2270 3685 5867 6363 2226 Size: 44 1415 2182 496 2527 Fragments arranged by size: 2527 2182 1415 496 44 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2099 2723 1163 Size: 573 363 624 5104 Fragments arranged by size: 5104 624 573 363 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4114 4875 5903 1540 Size: 275 186 2113 761 1028 2301 Fragments arranged by size: 2301 2113 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2799 2823 2844 3636 4725 4810 5881 2799 Size: 24 21 792 1089 85 1071 3582 Fragments arranged by size: 3582 1089 1071 792 85 24 21 BsmI GAATG_Cn' Cuts at: 2066 4175 4268 2066 Size: 2109 93 4462 Fragments arranged by size: 4462 2109 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2277 2370 2799 2823 2844 2990 3581 Size: 399 487 93 429 24 21 146 591 Cuts at: 3581 3636 3983 4725 4810 5881 1391 Size: 55 347 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 591 487 429 399 347 146 93 85 55 24 21 BspEI T'CCGG_A Cuts at: 6327 6327 Size: 6664 BspHI T'CATG_A Cuts at: 4553 6283 4553 Size: 1730 4934 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2691 3031 4019 844 Size: 1847 340 988 3489 Fragments arranged by size: 3489 1847 988 340 BsrBI CCG'CTC Cuts at: 1394 1823 2437 4551 1394 Size: 429 614 2114 3507 Fragments arranged by size: 3507 2114 614 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2691 3315 3735 3916 5159 5333 65 Size: 1183 1443 624 420 181 1243 174 1396 Fragments arranged by size: 1443 1396 1243 1183 624 420 181 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5308 5495 1700 Size: 110 3498 187 2869 Fragments arranged by size: 3498 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2405 96 Size: 2309 4355 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6664 BssSI C'ACGA_G Cuts at: 3759 4724 5736 3759 Size: 965 1012 4687 Fragments arranged by size: 4687 1012 965 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6664 BstBI TT'CG_AA Cuts at: 5468 5468 Size: 6664 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6664 BstXI CCAn_nnnn'nTGG Cuts at: 2074 2567 2074 Size: 493 6171 BstYI r'GATC_y Cuts at: 1972 3277 3927 3994 4361 4746 4763 6215 6301 Size: 1305 650 67 367 385 17 1452 86 Cuts at: 6301 6313 6659 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1305 650 385 367 346 86 67 17 12 BstZ17I GTA'TAC Cuts at: 3188 3188 Size: 6664 Bsu36I CC'TnA_GG Cuts at: 3042 3813 3882 3042 Size: 771 69 5824 Fragments arranged by size: 5824 771 69 BtgI C'CryG_G Cuts at: 513 1065 2004 2928 3172 3419 513 Size: 552 939 924 244 247 3758 Fragments arranged by size: 3758 939 924 552 247 244 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5925 Fragments arranged by size: 5925 683 56 BtsI GCAGTG_nn' Cuts at: 2799 3071 3382 4180 4974 4994 2799 Size: 272 311 798 794 20 4469 Fragments arranged by size: 4469 798 794 311 272 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6664 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6629 DraI TTT'AAA Cuts at: 3968 3968 Size: 6664 DraIII CAC_nnn'GTG Cuts at: 3388 3388 Size: 6664 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3855 5671 809 Size: 507 2539 1816 1802 Fragments arranged by size: 2539 1816 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2122 2160 2398 Size: 54 1425 50 275 18 292 38 238 Cuts at: 2398 2733 3379 3445 4111 5000 6629 8 Size: 335 646 66 666 889 1629 43 Fragments arranged by size: 1629 1425 889 666 646 335 292 275 238 66 54 50 43 38 18 EagI C'GGCC_G Cuts at: 1537 1812 4111 1537 Size: 275 2299 4090 Fragments arranged by size: 4090 2299 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2085 4594 1353 Size: 732 2509 3423 Fragments arranged by size: 3423 2509 732 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2515 3215 5251 5625 5771 387 Size: 1064 138 926 700 2036 374 146 1280 Fragments arranged by size: 2036 1280 1064 926 700 374 146 138 EcoICRI GAG'CTC Cuts at: 2797 2821 2842 2797 Size: 24 21 6619 Fragments arranged by size: 6619 24 21 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2168 2519 2543 2658 2786 3090 3635 Size: 393 536 351 24 115 128 304 545 Cuts at: 3635 3662 4727 5324 6111 6379 1239 Size: 27 1065 597 787 268 1524 Fragments arranged by size: 1524 1065 787 597 545 536 393 351 304 268 128 115 27 24 EcoNI CCTnn'n_nnAGG Cuts at: 3397 3397 Size: 6664 EcoO109I rG'GnC_Cy Cuts at: 2530 3015 3038 3671 2530 Size: 485 23 633 5523 Fragments arranged by size: 5523 633 485 23 EcoRI G'AATT_C Cuts at: 3973 4351 3973 Size: 378 6286 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2527 2527 Size: 6664 FspI TGC'GCA Cuts at: 5170 5170 Size: 6664 HaeII r_GCGC'y Cuts at: 1678 5811 1678 Size: 4133 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2224 2256 3491 3523 5305 Size: 32 135 32 652 32 1235 32 1782 Cuts at: 5305 5337 5379 5411 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1782 1235 652 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2463 2908 4008 4254 669 Size: 988 281 525 445 1100 246 3079 Fragments arranged by size: 3079 1100 988 525 445 281 246 HindIII A'AGCT_T Cuts at: 748 2108 3262 3479 748 Size: 1360 1154 217 3933 Fragments arranged by size: 3933 1360 1154 217 HpaI GTT'AAC Cuts at: 2463 2908 4254 2463 Size: 445 1346 4873 Fragments arranged by size: 4873 1346 445 KpnI G_GTAC'C Cuts at: 3989 3989 Size: 6664 MfeI C'AATT_G Cuts at: 4263 4263 Size: 6664 MluI A'CGCG_T Cuts at: 6540 6540 Size: 6664 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5778 5962 1305 Size: 4473 184 2007 Fragments arranged by size: 4473 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2124 2162 2400 2735 3381 Size: 54 1425 343 292 38 238 335 646 Cuts at: 3381 3447 10 Size: 66 3227 Fragments arranged by size: 3227 1425 646 343 335 292 238 66 54 38 MslI CAynn'nnrTG Cuts at: 518 1166 2072 3749 4622 4981 5140 518 Size: 648 906 1677 873 359 159 2042 Fragments arranged by size: 2042 1677 906 873 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2098 2184 2230 2270 2429 2497 2777 3034 Size: 492 86 46 40 159 68 280 257 Cuts at: 3034 3409 3528 3685 3732 3892 4080 4757 5905 Size: 375 119 157 47 160 188 677 1148 Cuts at: 5905 6150 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 492 375 280 257 245 188 160 159 157 119 86 68 47 46 40 NcoI C'CATG_G Cuts at: 513 1065 2004 2928 3419 513 Size: 552 939 924 491 3758 Fragments arranged by size: 3758 939 924 552 491 NdeI CA'TA_TG Cuts at: 387 387 Size: 6664 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6664 NotI GC'GGCC_GC Cuts at: 4111 4111 Size: 6664 NspI r_CATG'y Cuts at: 1132 1229 3748 4022 5567 1132 Size: 97 2519 274 1545 2229 Fragments arranged by size: 2519 2229 1545 274 97 PasI CC'CwG_GG Cuts at: 1348 3595 1348 Size: 2247 4417 PciI A'CATG_T Cuts at: 5563 5563 Size: 6664 PflMI CCAn_nnn'nTGG Cuts at: 1108 2146 3388 1108 Size: 1038 1242 4384 Fragments arranged by size: 4384 1242 1038 PfoI T'CCnGG_A Cuts at: 2046 2559 2589 3106 2046 Size: 513 30 517 5604 Fragments arranged by size: 5604 517 513 30 PmeI GTTT'AAAC Cuts at: 3968 3968 Size: 6664 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4733 4765 6246 6278 4733 Size: 32 1481 32 5119 Fragments arranged by size: 5119 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2530 3038 2530 Size: 508 6156 PshAI GACnn'nnGTC Cuts at: 1613 3786 1613 Size: 2173 4491 PsiI TTA'TAA Cuts at: 4234 4234 Size: 6664 PstI C_TGCA'G Cuts at: 830 2014 2496 4016 830 Size: 1184 482 1520 3478 Fragments arranged by size: 3478 1520 1184 482 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6664 PvuII CAG'CTG Cuts at: 2098 2230 2270 2429 2777 3409 3528 3685 3892 Size: 132 40 159 348 632 119 157 207 Cuts at: 3892 2098 Size: 4870 Fragments arranged by size: 4870 632 348 207 159 157 132 119 40 SacI G_AGCT'C Cuts at: 2799 2823 2844 2799 Size: 24 21 6619 Fragments arranged by size: 6619 24 21 SalI G'TCGA_C Cuts at: 1936 4006 1936 Size: 2070 4594 SapI GCTCTTCn'nnn_ Cuts at: 2085 2085 Size: 6664 SbfI CC_TGCA'GG Cuts at: 4016 4016 Size: 6664 ScaI AGT'ACT Cuts at: 3159 4912 6624 3159 Size: 1753 1712 3199 Fragments arranged by size: 3199 1753 1712 SfcI C'TryA_G Cuts at: 826 1045 2010 2492 3666 3826 3931 4012 5147 Size: 219 965 482 1174 160 105 81 1135 Cuts at: 5147 5828 6019 826 Size: 681 191 1471 Fragments arranged by size: 1471 1174 1135 965 681 482 219 191 160 105 81 SfiI GGCCn_nnn'nGGCC Cuts at: 2933 2933 Size: 6664 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6664 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6664 SmaI CCC'GGG Cuts at: 1872 3991 1872 Size: 2119 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2846 3077 3359 4768 5669 5931 Size: 197 926 903 231 282 1409 901 262 Cuts at: 5931 820 Size: 1553 Fragments arranged by size: 1553 1409 926 903 901 282 262 231 197 SnaBI TAC'GTA Cuts at: 493 4455 493 Size: 3962 2702 SpeI A'CTAG_T Cuts at: 152 3160 152 Size: 3008 3656 SphI G_CATG'C Cuts at: 1132 4022 1132 Size: 2890 3774 SspI AAT'ATT Cuts at: 5 52 4588 5 Size: 47 4536 2081 Fragments arranged by size: 4536 2081 47 StuI AGG'CCT Cuts at: 1517 3606 1517 Size: 2089 4575 StyI C'CwwG_G Cuts at: 513 1065 2004 2511 2526 2928 3011 3268 3372 Size: 552 939 507 15 402 83 257 104 Cuts at: 3372 3419 4103 513 Size: 47 684 3074 Fragments arranged by size: 3074 939 684 552 507 402 257 104 83 47 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2295 3608 4691 4708 4861 5046 5491 Size: 1255 715 1313 1083 17 153 185 445 Cuts at: 5491 325 Size: 1498 Fragments arranged by size: 1498 1313 1255 1083 715 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2405 3157 4910 Size: 274 80 33 51 1443 428 752 1753 Cuts at: 4910 6622 96 Size: 1712 138 Fragments arranged by size: 1753 1712 1443 752 428 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3498 3530 6624 6656 3498 Size: 32 3094 32 3506 Fragments arranged by size: 3506 3094 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6664 XbaI T'CTAG_A Cuts at: 4000 4000 Size: 6664 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2393 2659 3326 3644 2393 Size: 266 667 318 5413 Fragments arranged by size: 5413 667 318 266 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6664 XmaI C'CCGG_G Cuts at: 1870 3989 1870 Size: 2119 4545 XmnI GAAnn'nnTTC Cuts at: 2618 3952 4793 2618 Size: 1334 841 4489 Fragments arranged by size: 4489 1334 841 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4472 276 Size: 53 83 186 936 2938 2468 Fragments arranged by size: 2938 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbeI BfrBI BsiWI BsmBI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfoI SrfI SwaI