(Circular) (Six-base) MAPSORT of: pFN21AB9883.seq Check: 3022 from: 1 to: 6574 pFN21AB9883 6574 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4384 278 Size: 53 83 186 936 2848 2468 Fragments arranged by size: 2848 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3917 1937 Size: 1980 4594 Acc65I G'GTAC_C Cuts at: 3895 3895 Size: 6574 AclI AA'CG_TT Cuts at: 1563 2287 4701 5074 1563 Size: 724 2414 373 3063 Fragments arranged by size: 3063 2414 724 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 1999 2753 2885 4637 6021 6289 1632 Size: 367 754 132 1752 1384 268 1917 Fragments arranged by size: 1917 1752 1384 754 367 268 132 AfeI AGC'GCT Cuts at: 2870 2870 Size: 6574 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6377 AflIII A'CryG_T Cuts at: 1203 5473 6450 1203 Size: 4270 977 1327 Fragments arranged by size: 4270 1327 977 AgeI A'CCGG_T Cuts at: 5405 5405 Size: 6574 AhdI GACnn_n'nnGTC Cuts at: 5303 5303 Size: 6574 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6574 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2723 3252 1184 Size: 1539 529 4506 Fragments arranged by size: 4506 1539 529 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3354 3386 3354 Size: 32 6542 AlwNI CAG_nnn'CTG Cuts at: 2095 2731 3400 4317 5889 2095 Size: 636 669 917 1572 2780 Fragments arranged by size: 2780 1572 917 669 636 ApaLI G'TGCA_C Cuts at: 3566 4631 5787 3566 Size: 1065 1156 4353 Fragments arranged by size: 4353 1156 1065 ApoI r'AATT_y Cuts at: 1493 3516 3883 4109 4261 6370 1493 Size: 2023 367 226 152 2109 1697 Fragments arranged by size: 2109 2023 1697 367 226 152 AseI AT'TA_AT Cuts at: 160 1032 5128 160 Size: 872 4096 1606 Fragments arranged by size: 4096 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6574 AvaI C'yCGr_G Cuts at: 1870 1943 2136 3025 3899 1870 Size: 73 193 889 874 4545 Fragments arranged by size: 4545 889 874 193 73 BamHI G'GATC_C Cuts at: 3904 4271 3904 Size: 367 6207 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2316 3135 3228 3895 5350 Size: 325 217 627 529 819 93 667 1455 Cuts at: 5350 618 Size: 1842 Fragments arranged by size: 1842 1455 819 667 627 529 325 217 93 BanII G_rGCy'C Cuts at: 1391 2717 2978 3071 3281 3342 3609 3893 1391 Size: 1326 261 93 210 61 267 284 4072 Fragments arranged by size: 4072 1326 284 267 261 210 93 61 BbeI G_GCGC'C Cuts at: 2320 3139 2320 Size: 819 5755 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2574 928 Size: 971 675 4928 Fragments arranged by size: 4928 971 675 BbvCI CC'TCA_GC Cuts at: 3333 3425 3333 Size: 92 6482 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3105 3139 4765 4799 1903 Size: 34 1168 34 1626 34 3678 Fragments arranged by size: 3678 1626 1168 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2814 4466 5676 2814 Size: 1652 1210 3712 Fragments arranged by size: 3712 1652 1210 BclI T'GATC_A Cuts at: 1548 3820 1548 Size: 2272 4302 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2218 5185 136 Size: 107 122 71 631 605 546 2967 1525 Fragments arranged by size: 2967 1525 631 605 546 122 107 71 BglII A'GATC_T Cuts at: 6569 6569 Size: 6574 BlpI GC'TnA_GC Cuts at: 3991 3991 Size: 6574 Bme1580I G_kGCm'C Cuts at: 1790 2592 3024 3570 4635 5791 1790 Size: 802 432 546 1065 1156 2573 Fragments arranged by size: 2573 1156 1065 802 546 432 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6574 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4305 5263 442 Size: 416 53 83 399 24 2888 958 1753 Fragments arranged by size: 2888 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6574 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3819 3819 Size: 6574 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2680 2918 2954 3107 3766 5234 1239 Size: 1441 238 36 153 659 1468 2579 Fragments arranged by size: 2579 1468 1441 659 238 153 36 Bpu10I CC'TnA_GC Cuts at: 2258 2847 2979 3275 3333 3425 2258 Size: 589 132 296 58 92 5407 Fragments arranged by size: 5407 589 296 132 92 58 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2789 4699 5564 5862 2789 Size: 1910 865 298 3501 Fragments arranged by size: 3501 1910 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2505 5237 882 Size: 617 1006 2732 2219 Fragments arranged by size: 2732 2219 1006 617 BsaAI yAC'GTr Cuts at: 493 1204 3565 4365 493 Size: 711 2361 800 2702 Fragments arranged by size: 2702 2361 800 711 BsaBI GATnn'nnATC Cuts at: 6568 6568 Size: 6574 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2317 3136 4381 4763 Size: 53 83 186 936 784 819 1245 382 Cuts at: 4763 5398 275 Size: 635 1451 Fragments arranged by size: 1451 1245 936 819 784 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2111 5007 5405 5679 5826 6237 2111 Size: 2896 398 274 147 411 2448 Fragments arranged by size: 2896 2448 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3153 3183 3263 3293 3653 Size: 30 165 30 1750 30 80 30 360 Cuts at: 3653 3683 1178 Size: 30 4069 Fragments arranged by size: 4069 1750 360 165 80 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2472 2873 3214 3350 3806 1186 Size: 1286 401 341 136 456 3954 Fragments arranged by size: 3954 1286 456 401 341 136 BseYI C'CCAG_C Cuts at: 2028 2095 2168 2379 2391 2682 3840 5777 6273 Size: 67 73 211 12 291 1158 1937 496 Cuts at: 6273 2028 Size: 2329 Fragments arranged by size: 2329 1937 1158 496 291 211 73 67 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2168 3611 3740 1163 Size: 573 432 1443 129 3997 Fragments arranged by size: 3997 1443 573 432 129 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2399 4024 4785 5813 1540 Size: 275 186 398 1625 761 1028 2301 Fragments arranged by size: 2301 1625 1028 761 398 275 186 BsiHKAI G_wGCw'C Cuts at: 2135 2231 3114 3281 3342 3570 3609 4635 4720 Size: 96 883 167 61 228 39 1026 85 Cuts at: 4720 5791 2135 Size: 1071 2918 Fragments arranged by size: 2918 1071 1026 883 228 167 96 85 61 39 BsmI GAATG_Cn' Cuts at: 4085 4178 4085 Size: 93 6481 BsmBI CGTCTCn'nnnn_ Cuts at: 2807 2807 Size: 6574 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2135 2231 2592 2717 2978 3024 3071 Size: 399 345 96 361 125 261 46 47 Cuts at: 3071 3114 3281 3342 3570 3609 3893 4635 4720 Size: 43 167 61 228 39 284 742 85 Cuts at: 4720 5791 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 361 345 284 261 228 167 125 96 85 61 47 46 43 39 BspEI T'CCGG_A Cuts at: 2111 6237 2111 Size: 4126 2448 BspHI T'CATG_A Cuts at: 4463 6193 4463 Size: 1730 4844 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3929 844 Size: 3085 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2578 2902 3412 4461 1394 Size: 429 755 324 510 1049 3507 Fragments arranged by size: 3507 1049 755 510 429 324 BsrDI GCAATG_nn' Cuts at: 65 1248 5069 5243 65 Size: 1183 3821 174 1396 Fragments arranged by size: 3821 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2301 2910 3036 5218 5405 1700 Size: 110 491 609 126 2182 187 2869 Fragments arranged by size: 2869 2182 609 491 187 126 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6574 BssHII G'CGCG_C Cuts at: 1925 3095 3097 3475 1925 Size: 1170 2 378 5024 Fragments arranged by size: 5024 1170 378 2 BssSI C'ACGA_G Cuts at: 4634 5646 4634 Size: 1012 5562 BstAPI GCAn_nnn'nTGC Cuts at: 794 3187 3718 794 Size: 2393 531 3650 Fragments arranged by size: 3650 2393 531 BstBI TT'CG_AA Cuts at: 5378 5378 Size: 6574 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6574 BstYI r'GATC_y Cuts at: 1972 2044 3543 3904 4271 4656 4673 6125 6211 Size: 72 1499 361 367 385 17 1452 86 Cuts at: 6211 6223 6569 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1499 1452 385 367 361 346 86 72 17 12 Bsu36I CC'TnA_GG Cuts at: 3344 3401 3617 3774 3344 Size: 57 216 157 6144 Fragments arranged by size: 6144 216 157 57 BtgI C'CryG_G Cuts at: 513 1065 2004 2060 2207 3240 513 Size: 552 939 56 147 1033 3847 Fragments arranged by size: 3847 1033 939 552 147 56 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2277 487 Size: 683 56 1051 4784 Fragments arranged by size: 4784 1051 683 56 BtsI GCAGTG_nn' Cuts at: 2237 2529 2945 4090 4884 4904 2237 Size: 292 416 1145 794 20 3907 Fragments arranged by size: 3907 1145 794 416 292 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6574 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6539 DraI TTT'AAA Cuts at: 3878 3878 Size: 6574 DraIII CAC_nnn'GTG Cuts at: 2130 2507 2130 Size: 377 6197 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2544 5581 809 Size: 507 1228 3037 1802 Fragments arranged by size: 3037 1802 1228 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2396 4021 4910 Size: 54 1425 50 275 18 566 1625 889 Cuts at: 4910 6539 8 Size: 1629 43 Fragments arranged by size: 1629 1625 1425 889 566 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2396 4021 1537 Size: 275 584 1625 4090 Fragments arranged by size: 4090 1625 584 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2630 2729 2742 3444 4504 1353 Size: 1277 99 13 702 1060 3423 Fragments arranged by size: 3423 1277 1060 702 99 13 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2085 3548 5161 5535 5681 387 Size: 1064 138 496 1463 1613 374 146 1280 Fragments arranged by size: 1613 1463 1280 1064 496 374 146 138 EcoICRI GAG'CTC Cuts at: 3279 3340 3607 3279 Size: 61 267 6246 Fragments arranged by size: 6246 267 61 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1999 2680 2753 2885 2918 2954 3107 Size: 393 367 681 73 132 33 36 153 Cuts at: 3107 3766 4637 5234 6021 6289 1239 Size: 659 871 597 787 268 1524 Fragments arranged by size: 1524 871 787 681 659 597 393 367 268 153 132 73 36 33 EcoNI CCTnn'n_nnAGG Cuts at: 3404 3404 Size: 6574 EcoO109I rG'GnC_Cy Cuts at: 2277 2728 3307 3397 2277 Size: 451 579 90 5454 Fragments arranged by size: 5454 579 451 90 EcoRI G'AATT_C Cuts at: 3516 3883 4261 3516 Size: 367 378 5829 Fragments arranged by size: 5829 378 367 FspI TGC'GCA Cuts at: 3509 5080 3509 Size: 1571 5003 HaeII r_GCGC'y Cuts at: 1678 2320 2872 2911 3139 3803 5721 1678 Size: 642 552 39 228 664 1918 2531 Fragments arranged by size: 2531 1918 664 642 552 228 39 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5215 5247 5289 5321 1373 Size: 32 135 32 3643 32 42 32 2626 Fragments arranged by size: 3643 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3918 4164 669 Size: 988 281 1980 246 3079 Fragments arranged by size: 3079 1980 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6574 HpaI GTT'AAC Cuts at: 4164 4164 Size: 6574 KasI G'GCGC_C Cuts at: 2316 3135 2316 Size: 819 5755 KpnI G_GTAC'C Cuts at: 3899 3899 Size: 6574 MfeI C'AATT_G Cuts at: 4173 4173 Size: 6574 MluI A'CGCG_T Cuts at: 6450 6450 Size: 6574 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3571 5688 5872 1305 Size: 2266 2117 184 2007 Fragments arranged by size: 2266 2117 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4752 Fragments arranged by size: 4752 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3234 4532 4891 5050 518 Size: 648 2068 1298 359 159 2042 Fragments arranged by size: 2068 2042 1298 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2062 2092 2209 2383 2395 3193 3352 3376 Size: 456 30 117 174 12 798 159 24 Cuts at: 3376 3990 4667 5815 6060 1606 Size: 614 677 1148 245 2120 Fragments arranged by size: 2120 1148 798 677 614 456 245 174 159 117 30 24 12 NaeI GCC'GGC Cuts at: 2912 2912 Size: 6574 NarI GG'CG_CC Cuts at: 2317 3136 2317 Size: 819 5755 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5083 Fragments arranged by size: 5083 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6574 NgoMIV G'CCGG_C Cuts at: 2910 2910 Size: 6574 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6574 NotI GC'GGCC_GC Cuts at: 2396 4021 2396 Size: 1625 4949 NspI r_CATG'y Cuts at: 1132 1229 3239 3932 5477 1132 Size: 97 2010 693 1545 2229 Fragments arranged by size: 2229 2010 1545 693 97 PasI CC'CwG_GG Cuts at: 1348 2428 1348 Size: 1080 5494 PciI A'CATG_T Cuts at: 5473 5473 Size: 6574 PflMI CCAn_nnn'nTGG Cuts at: 1108 2689 1108 Size: 1581 4993 PfoI T'CCnGG_A Cuts at: 2291 3171 3261 3600 3753 3782 2291 Size: 880 90 339 153 29 5083 Fragments arranged by size: 5083 880 339 153 90 29 PmeI GTTT'AAAC Cuts at: 3878 3878 Size: 6574 PmlI CAC'GTG Cuts at: 3565 3565 Size: 6574 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4643 4675 6156 6188 4643 Size: 32 1481 32 5029 Fragments arranged by size: 5029 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2728 3307 2728 Size: 579 5995 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6574 PsiI TTA'TAA Cuts at: 4144 4144 Size: 6574 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3854 3886 3854 Size: 32 6542 PstI C_TGCA'G Cuts at: 830 2835 3192 3198 3926 830 Size: 2005 357 6 728 3478 Fragments arranged by size: 3478 2005 728 357 6 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6574 PvuII CAG'CTG Cuts at: 2383 3193 3352 2383 Size: 810 159 5605 Fragments arranged by size: 5605 810 159 SacI G_AGCT'C Cuts at: 3281 3342 3609 3281 Size: 61 267 6246 Fragments arranged by size: 6246 267 61 SacII CC_GC'GG Cuts at: 2063 2210 2063 Size: 147 6427 SalI G'TCGA_C Cuts at: 1936 3916 1936 Size: 1980 4594 SapI GCTCTTCn'nnn_ Cuts at: 2729 3444 2729 Size: 715 5859 SbfI CC_TGCA'GG Cuts at: 3926 3926 Size: 6574 ScaI AGT'ACT Cuts at: 4822 6534 4822 Size: 1712 4862 SexAI A'CCwGG_T Cuts at: 2652 2652 Size: 6574 SfcI C'TryA_G Cuts at: 826 1045 2831 3188 3194 3922 5057 5738 5929 Size: 219 1786 357 6 728 1135 681 191 Cuts at: 5929 826 Size: 1471 Fragments arranged by size: 1786 1471 1135 728 681 357 219 191 6 SfoI GGC'GCC Cuts at: 2318 3137 2318 Size: 819 5755 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6574 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6574 SmaI CCC'GGG Cuts at: 1872 2138 3027 3901 1872 Size: 266 889 874 4545 Fragments arranged by size: 4545 889 874 266 SmlI C'TyrA_G Cuts at: 820 1017 1943 2804 4678 5579 5841 820 Size: 197 926 861 1874 901 262 1553 Fragments arranged by size: 1874 1553 926 901 861 262 197 SnaBI TAC'GTA Cuts at: 493 4365 493 Size: 3872 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6574 SphI G_CATG'C Cuts at: 1132 3932 1132 Size: 2800 3774 SspI AAT'ATT Cuts at: 5 52 4498 5 Size: 47 4446 2081 Fragments arranged by size: 4446 2081 47 StuI AGG'CCT Cuts at: 1517 2852 3779 1517 Size: 1335 927 4312 Fragments arranged by size: 4312 1335 927 StyI C'CwwG_G Cuts at: 513 1065 2004 2688 3327 3733 4013 513 Size: 552 939 684 639 406 280 3074 Fragments arranged by size: 3074 939 684 639 552 406 280 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2738 4601 4618 4771 4956 5401 325 Size: 1255 1158 1863 17 153 185 445 1498 Fragments arranged by size: 1863 1498 1255 1158 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4820 6532 96 Size: 274 80 33 51 1443 2843 1712 138 Fragments arranged by size: 2843 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2137 2169 3023 3055 3653 3685 6534 6566 2137 Size: 32 854 32 598 32 2849 32 2145 Fragments arranged by size: 2849 2145 854 598 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6574 XbaI T'CTAG_A Cuts at: 3910 3910 Size: 6574 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2092 2092 Size: 6574 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6574 XmaI C'CCGG_G Cuts at: 1870 2136 3025 3899 1870 Size: 266 889 874 4545 Fragments arranged by size: 4545 889 874 266 XmnI GAAnn'nnTTC Cuts at: 4703 4703 Size: 6574 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4382 276 Size: 53 83 186 936 2848 2468 Fragments arranged by size: 2848 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I EcoRV FalI FseI FspAI NruI NsiI PacI PspOMI PspXI RsrII SanDI SfiI SrfI SwaI