(Circular) (Six-base) MAPSORT of: pFN21AB9875.seq Check: 9263 from: 1 to: 6286 pFN21AB9875 6286 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4096 278 Size: 53 83 186 936 2560 2468 Fragments arranged by size: 2560 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3629 1937 Size: 1692 4594 Acc65I G'GTAC_C Cuts at: 3295 3607 3295 Size: 312 5974 AclI AA'CG_TT Cuts at: 1563 4413 4786 1563 Size: 2850 373 3063 Fragments arranged by size: 3063 2850 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2195 2427 2588 3078 3168 4349 5733 6001 Size: 563 232 161 490 90 1181 1384 268 Cuts at: 6001 1632 Size: 1917 Fragments arranged by size: 1917 1384 1181 563 490 268 232 161 90 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6089 AflIII A'CryG_T Cuts at: 1203 5185 6162 1203 Size: 3982 977 1327 Fragments arranged by size: 3982 1327 977 AgeI A'CCGG_T Cuts at: 5117 5117 Size: 6286 AhdI GACnn_n'nnGTC Cuts at: 5015 5015 Size: 6286 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6286 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2774 1184 Size: 1590 4696 AlwNI CAG_nnn'CTG Cuts at: 4029 5601 4029 Size: 1572 4714 ApaLI G'TGCA_C Cuts at: 4343 5499 4343 Size: 1156 5130 ApoI r'AATT_y Cuts at: 1493 2071 2567 2813 2898 3152 3197 3595 3821 Size: 578 496 246 85 254 45 398 226 Cuts at: 3821 3973 6082 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 578 496 398 254 246 226 152 85 45 AseI AT'TA_AT Cuts at: 160 1032 2430 4840 160 Size: 872 1398 2410 1606 Fragments arranged by size: 2410 1606 1398 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6286 AvaI C'yCGr_G Cuts at: 1870 1943 3611 1870 Size: 73 1668 4545 Fragments arranged by size: 4545 1668 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3279 3312 3279 Size: 33 6253 BamHI G'GATC_C Cuts at: 3616 3983 3616 Size: 367 5919 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3295 3607 5062 618 Size: 325 217 627 1508 312 1455 1842 Fragments arranged by size: 1842 1508 1455 627 325 312 217 BanII G_rGCy'C Cuts at: 1391 3318 3466 3605 1391 Size: 1927 148 139 4072 Fragments arranged by size: 4072 1927 148 139 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3147 928 Size: 971 1248 4067 Fragments arranged by size: 4067 1248 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4477 4511 1903 Size: 34 2540 34 3678 Fragments arranged by size: 3678 2540 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4178 5388 4178 Size: 1210 5076 BclI T'GATC_A Cuts at: 1548 1548 Size: 6286 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4897 136 Size: 107 122 71 631 605 3225 1525 Fragments arranged by size: 3225 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6281 6281 Size: 6286 BlpI GC'TnA_GC Cuts at: 3703 3703 Size: 6286 Bme1580I G_kGCm'C Cuts at: 1790 3360 4347 5503 1790 Size: 1570 987 1156 2573 Fragments arranged by size: 2573 1570 1156 987 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6286 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3370 4017 4975 Size: 416 53 83 399 24 1953 647 958 Cuts at: 4975 442 Size: 1753 Fragments arranged by size: 1953 1753 958 647 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6286 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2154 2764 4946 1239 Size: 915 610 2182 2579 Fragments arranged by size: 2579 2182 915 610 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4411 5276 5574 4411 Size: 865 298 5123 Fragments arranged by size: 5123 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2906 4949 882 Size: 617 1407 2043 2219 Fragments arranged by size: 2219 2043 1407 617 BsaAI yAC'GTr Cuts at: 493 1204 4077 493 Size: 711 2873 2702 Fragments arranged by size: 2873 2702 711 BsaBI GATnn'nnATC Cuts at: 6280 6280 Size: 6286 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4093 4475 5110 275 Size: 53 83 186 936 2560 382 635 1451 Fragments arranged by size: 2560 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4719 5117 5391 5538 5949 4719 Size: 398 274 147 411 5056 Fragments arranged by size: 5056 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6061 Fragments arranged by size: 6061 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2205 1186 Size: 1019 5267 BseYI C'CCAG_C Cuts at: 2227 2737 5489 5985 2227 Size: 510 2752 496 2528 Fragments arranged by size: 2752 2528 510 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2359 3186 1163 Size: 573 623 827 4263 Fragments arranged by size: 4263 827 623 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3736 4497 5525 1540 Size: 275 186 1735 761 1028 2301 Fragments arranged by size: 2301 1735 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3318 3466 4347 4432 5503 3318 Size: 148 881 85 1071 4101 Fragments arranged by size: 4101 1071 881 148 85 BsmI GAATG_Cn' Cuts at: 3797 3890 3797 Size: 93 6193 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3318 3360 3466 3605 4347 4432 5503 Size: 399 1528 42 106 139 742 85 1071 Cuts at: 5503 1391 Size: 2174 Fragments arranged by size: 2174 1528 1071 742 399 139 106 85 42 BspEI T'CCGG_A Cuts at: 5949 5949 Size: 6286 BspHI T'CATG_A Cuts at: 4175 5905 4175 Size: 1730 4556 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3641 844 Size: 2797 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4173 1394 Size: 429 2350 3507 Fragments arranged by size: 3507 2350 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3445 4781 4955 65 Size: 1183 2197 1336 174 1396 Fragments arranged by size: 2197 1396 1336 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4930 5117 1700 Size: 110 3120 187 2869 Fragments arranged by size: 3120 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2439 2703 96 Size: 2343 264 3679 Fragments arranged by size: 3679 2343 264 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6286 BssSI C'ACGA_G Cuts at: 4346 5358 4346 Size: 1012 5274 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6286 BstBI TT'CG_AA Cuts at: 5090 5090 Size: 6286 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6286 BstXI CCAn_nnnn'nTGG Cuts at: 2434 2434 Size: 6286 BstYI r'GATC_y Cuts at: 1972 2120 3115 3382 3616 3983 4368 4385 5837 Size: 148 995 267 234 367 385 17 1452 Cuts at: 5837 5923 5935 6281 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 995 385 367 346 267 234 148 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2284 513 Size: 552 1219 4515 Fragments arranged by size: 4515 1219 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5547 Fragments arranged by size: 5547 683 56 BtsI GCAGTG_nn' Cuts at: 3802 4596 4616 3802 Size: 794 20 5472 Fragments arranged by size: 5472 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6286 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3118 3153 529 Size: 35 2554 35 3662 Fragments arranged by size: 3662 2554 35 35 DraI TTT'AAA Cuts at: 3590 3590 Size: 6286 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5293 809 Size: 507 3977 1802 Fragments arranged by size: 3977 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3733 4622 6251 Size: 54 1425 50 275 18 1903 889 1629 Cuts at: 6251 8 Size: 43 Fragments arranged by size: 1903 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3733 1537 Size: 275 1921 4090 Fragments arranged by size: 4090 1921 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2223 3069 4216 1353 Size: 870 846 1147 3423 Fragments arranged by size: 3423 1147 870 846 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4873 5247 5393 387 Size: 1064 138 3284 374 146 1280 Fragments arranged by size: 3284 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3316 3464 3316 Size: 148 6138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2154 2195 2427 2588 2764 3078 3168 Size: 393 522 41 232 161 176 314 90 Cuts at: 3168 4349 4946 5733 6001 1239 Size: 1181 597 787 268 1524 Fragments arranged by size: 1524 1181 787 597 522 393 314 268 232 176 161 90 41 EcoRI G'AATT_C Cuts at: 3595 3973 3595 Size: 378 5908 EcoRV GAT'ATC Cuts at: 3283 3283 Size: 6286 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3580 3580 Size: 6286 FspI TGC'GCA Cuts at: 4792 4792 Size: 6286 HaeII r_GCGC'y Cuts at: 1678 5433 1678 Size: 3755 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2600 2632 4927 4959 5001 Size: 32 135 32 1028 32 2295 32 42 Cuts at: 5001 5033 1373 Size: 32 2626 Fragments arranged by size: 2626 2295 1028 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3630 3876 669 Size: 988 281 1692 246 3079 Fragments arranged by size: 3079 1692 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6286 HpaI GTT'AAC Cuts at: 3876 3876 Size: 6286 KpnI G_GTAC'C Cuts at: 3299 3611 3299 Size: 312 5974 MfeI C'AATT_G Cuts at: 3885 3885 Size: 6286 MluI A'CGCG_T Cuts at: 6162 6162 Size: 6286 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5400 5584 1305 Size: 4095 184 2007 Fragments arranged by size: 4095 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4464 Fragments arranged by size: 4464 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2148 2163 2432 3351 4244 4603 4762 Size: 648 982 15 269 919 893 359 159 Cuts at: 4762 518 Size: 2042 Fragments arranged by size: 2042 982 919 893 648 359 269 159 15 MspA1I CmG'CkG Cuts at: 1606 2741 3702 4379 5527 5772 1606 Size: 1135 961 677 1148 245 2120 Fragments arranged by size: 2120 1148 1135 961 677 245 NcoI C'CATG_G Cuts at: 513 1065 2284 513 Size: 552 1219 4515 Fragments arranged by size: 4515 1219 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6286 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6286 NotI GC'GGCC_GC Cuts at: 3733 3733 Size: 6286 NspI r_CATG'y Cuts at: 1132 1229 2491 3261 3449 3644 5189 1132 Size: 97 1262 770 188 195 1545 2229 Fragments arranged by size: 2229 1545 1262 770 195 188 97 PasI CC'CwG_GG Cuts at: 1348 2728 1348 Size: 1380 4906 PciI A'CATG_T Cuts at: 5185 5185 Size: 6286 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6286 PmeI GTTT'AAAC Cuts at: 3590 3590 Size: 6286 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3384 3416 4355 4387 5868 5900 3384 Size: 32 939 32 1481 32 3770 Fragments arranged by size: 3770 1481 939 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6286 PsiI TTA'TAA Cuts at: 3856 3856 Size: 6286 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2385 2417 3514 3546 2385 Size: 32 1097 32 5125 Fragments arranged by size: 5125 1097 32 32 PstI C_TGCA'G Cuts at: 830 2016 3638 830 Size: 1186 1622 3478 Fragments arranged by size: 3478 1622 1186 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6286 PvuII CAG'CTG Cuts at: 2741 2741 Size: 6286 SacI G_AGCT'C Cuts at: 3318 3466 3318 Size: 148 6138 SalI G'TCGA_C Cuts at: 1936 3628 1936 Size: 1692 4594 SapI GCTCTTCn'nnn_ Cuts at: 3069 3069 Size: 6286 SbfI CC_TGCA'GG Cuts at: 3638 3638 Size: 6286 ScaI AGT'ACT Cuts at: 4534 6246 4534 Size: 1712 4574 SfcI C'TryA_G Cuts at: 826 1045 2012 3634 4769 5450 5641 826 Size: 219 967 1622 1135 681 191 1471 Fragments arranged by size: 1622 1471 1135 967 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6286 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6286 SmaI CCC'GGG Cuts at: 1872 3613 1872 Size: 1741 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4390 5291 5553 820 Size: 197 926 2447 901 262 1553 Fragments arranged by size: 2447 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4077 493 Size: 3584 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6286 SphI G_CATG'C Cuts at: 1132 3644 1132 Size: 2512 3774 SspI AAT'ATT Cuts at: 5 52 4210 5 Size: 47 4158 2081 Fragments arranged by size: 4158 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6286 StyI C'CwwG_G Cuts at: 513 1065 2284 3725 513 Size: 552 1219 1441 3074 Fragments arranged by size: 3074 1441 1219 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2450 2562 4313 4330 4483 4668 5113 Size: 1255 870 112 1751 17 153 185 445 Cuts at: 5113 325 Size: 1498 Fragments arranged by size: 1751 1498 1255 870 445 185 153 112 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2439 2703 4532 Size: 274 80 33 51 1443 462 264 1829 Cuts at: 4532 6244 96 Size: 1712 138 Fragments arranged by size: 1829 1712 1443 462 274 264 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6246 6278 6246 Size: 32 6254 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6286 XbaI T'CTAG_A Cuts at: 3622 3622 Size: 6286 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6286 XmaI C'CCGG_G Cuts at: 1870 3611 1870 Size: 1741 4545 XmnI GAAnn'nnTTC Cuts at: 4415 4415 Size: 6286 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4094 276 Size: 53 83 186 936 2560 2468 Fragments arranged by size: 2560 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI