Restriction map for
pFN21AB9868
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# NO CUT ENZYMES ( 109 enzymes) #
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AatII Acc65I AclI AflII AflIII
AgeI AhdI AlwNI AocI AscI
AseI AsnI Asp718I AspEI AvrII
BamHI BbrPI BbvCI BciVI BclI
BfaI BfrI BlnI BlpI Bpu1102I
BsaBI BsaI BsiWI BsmBI BsmI
Bsp1407I BspDI BspLU11I BsrDI BsrGI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DdeI DraI DrdI Eam1105I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI HincII HindII HindIII HinfI
HpaI KpnI MaeI MamI MfeI
MluI MseI MunI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SalI ScaI SexAI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI StuI SwaI TfiI Tru9I
TspRI Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 643 GTCTAC GT^[AC][GT]AC
AccIII 705 TCCGGA T^CCGGA
AcsI 1225 AAATTC [GA]^AATT[CT]
Alw44I 364 GTGCAC G^TGCAC
Aor51HI 1109 AGCGCT AGC^GCT
ApaLI 364 GTGCAC G^TGCAC
ApoI 1225 AAATTC [GA]^AATT[CT]
Asp700I 1107 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 709 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 650 TGCGCA TGC^GCA
BbsI 891 GTCTTC GAAGAC(2/6)
BcgI 149 GCAACGTGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 320 AGATCT A^GATCT
BpuAI 891 GTCTTC GAAGAC(2/6)
BsaAI 646 TACGTG [CT]AC^GT[GA]
BsaWI 705 TCCGGA [AT]^CCGG[AT]
BseAI 705 TCCGGA T^CCGGA
BsgI 348 CTGCAC GTGCAG(16/14)
BsmAI 219 GTCTC GTCTC(1/5)
BspEI 705 TCCGGA T^CCGGA
BspHI 632 TCATGA T^CATGA
BspMI 90 GCAGGT ACCTGC(4/8)
BssHII 185 GCGCGC G^CGCGC
BssSI 177 CACGAG CACGAG(-5/-1)
DraIII 465 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1109 AGCGCT AGC^GCT
EcoT14I 1070 CCAAGG C^C[AT][AT]GG
FseI 117 GGCCGGCC GGCCGG^CC
FspI 650 TGCGCA TGC^GCA
KspI 997 CCGCGG CCGC^GG
MaeIII 94 GTGAC ^GT[ACGT]AC
MroI 705 TCCGGA T^CCGGA
PflFI 709 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 632 TCATGA T^CATGA
SacI 885 GAGCTC GAGCT^C
SacII 997 CCGCGG CCGC^GG
SbfI 87 CCTGCAGG CCTGCA^GG
SfiI 911 GGCCTCCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 190 CTCAAG C^T[CT][GA]AG
Sse8387I 87 CCTGCAGG CCTGCA^GG
StyI 1070 CCAAGG C^C[AT][AT]GG
Tsp45I 94 GTGAC ^GT[GC]AC
Tsp509I 1226 AATT ^AATT
Tth111I 709 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 275 CCAACGAGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1107 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
BalI 245 TGGCCA TGG^CCA
272 TGGCCA
BpmI 914 CTCCAG CTGGAG(16/14)
877 CTGGAG
BseRI 880 GAGGAG GAGGAG(10/8)
1051 GAGGAG
BsrBI 502 CCGCTC CCGCTC(-3/-3)
907 GAGCGG
BsrI 293 ACTGG ACTGG(1/-1)
395 ACTGG
BstF5I 237 CATCC GGATG(2/0)
258 CATCC
BstXI 377 CCATCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
749 CCAACGGTCTGG
BstYI 320 AGATCT [GA]^GATC[CT]
1316 AGATCC
DsaI 997 CCGCGG C^C[GA][CT]GG
1130 CCGTGG
FokI 237 CATCC GGATG(9/13)
258 CATCC
MflI 320 AGATCT [GA]^GATC[CT]
1316 AGATCC
MluNI 245 TGGCCA TGG^CCA
272 TGGCCA
MscI 245 TGGCCA TGG^CCA
272 TGGCCA
MspA1I 802 CCGCTG C[AC]G^C[GT]G
997 CCGCGG
SapI 342 GCTCTTC GCTCTTC(1/4)
1218 GCTCTTC
XhoII 320 AGATCT [GA]^GATC[CT]
1316 AGATCC
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# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AspHI AvaI AvaII
BanI BanII BbeI BbvI BcnI
BglI BmyI BsaHI BsaJI BsiEI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrFI BssKI BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DpnII
DraII EaeI EagI EarI EclXI
Eco52I EcoO109I EcoRII FauI Fnu4HI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HpaII HphI ItaI
KasI Ksp632I MaeII MboI MboII
MnlI MslI MspI MvaI MvnI
MwoI NaeI NarI NciI NdeII
NgoMIV NlaIII NlaIV PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI TaqI TseI TthHB8I
XmaCI XmaI