Restriction map for
pFN21AB9864
(Linear) (Six-base) MAPSORT of: KIBB9864.seq Check: 5643 from: 1 to: 1266
KIBB9864.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AfeI AGC'GCT
Cuts at: 0 193 1266
Size: 193 1073
AflIII A'CryG_T
Cuts at: 0 1226 1266
Size: 1226 40
AlwNI CAG_nnn'CTG
Cuts at: 0 404 785 1266
Size: 404 381 481
Fragments arranged by size:
481 404 381
ApaLI G'TGCA_C
Cuts at: 0 883 1266
Size: 883 383
AscI GG'CGCG_CC
Cuts at: 0 453 1266
Size: 453 813
AvaI C'yCGr_G
Cuts at: 0 1013 1266
Size: 1013 253
BanI G'GyrC_C
Cuts at: 0 859 1054 1144 1266
Size: 859 195 90 122
Fragments arranged by size:
859 195 122 90
BbeI G_GCGC'C
Cuts at: 0 863 1148 1266
Size: 863 285 118
Fragments arranged by size:
863 285 118
BbsI GAAGACnn'nnnn_
Cuts at: 0 542 709 1030 1039 1266
Size: 542 167 321 9 227
Fragments arranged by size:
542 321 227 167 9
BclI T'GATC_A
Cuts at: 0 110 1266
Size: 110 1156
BlpI GC'TnA_GC
Cuts at: 0 10 1266
Size: 10 1256
Bme1580I G_kGCm'C
Cuts at: 0 133 887 951 1057 1266
Size: 133 754 64 106 209
Fragments arranged by size:
754 209 133 106 64
BmgBI CAC'GTC
Cuts at: 0 90 348 1266
Size: 90 258 918
Fragments arranged by size:
918 258 90
BmrI ACTGGGnnnn_n'
Cuts at: 0 319 414 1266
Size: 319 95 852
Fragments arranged by size:
852 319 95
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 807 889 1266
Size: 807 82 377
Fragments arranged by size:
807 377 82
BsaI GGTCTCn'nnnn_
Cuts at: 0 52 1266
Size: 52 1214
BsaAI yAC'GTr
Cuts at: 0 432 1266
Size: 432 834
BsaHI Gr'CG_yC
Cuts at: 0 445 860 1145 1266
Size: 445 415 285 121
Fragments arranged by size:
445 415 285 121
BsaWI w'CCGG_w
Cuts at: 0 706 1266
Size: 706 560
BseYI C'CCAG_C
Cuts at: 0 126 153 516 792 811 922 1038 1161
Size: 126 27 363 276 19 111 116 123
Cuts at: 1161 1266
Size: 105
Fragments arranged by size:
363 276 126 123 116 111 105 27
19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 342 1266
Size: 342 924
BsiHKAI G_wGCw'C
Cuts at: 0 11 296 822 887 911 1266
Size: 11 285 526 65 24 355
Fragments arranged by size:
526 355 285 65 24 11
Bsp1286I G_dGCh'C
Cuts at: 0 11 133 296 822 887 911 951 1057
Size: 11 122 163 526 65 24 40 106
Cuts at: 1057 1266
Size: 209
Fragments arranged by size:
526 209 163 122 106 65 40 24
11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 331 1266
Size: 331 935
BsrBI CCG'CTC
Cuts at: 0 542 712 1266
Size: 542 170 554
Fragments arranged by size:
554 542 170
BsrDI GCAATG_nn'
Cuts at: 0 165 1266
Size: 165 1101
BsrFI r'CCGG_y
Cuts at: 0 45 61 1266
Size: 45 16 1205
Fragments arranged by size:
1205 45 16
BssHII G'CGCG_C
Cuts at: 0 453 664 1266
Size: 453 211 602
Fragments arranged by size:
602 453 211
BstAPI GCAn_nnn'nTGC
Cuts at: 0 164 1266
Size: 164 1102
BstEII G'GTnAC_C
Cuts at: 0 237 318 1266
Size: 237 81 948
Fragments arranged by size:
948 237 81
BstXI CCAn_nnnn'nTGG
Cuts at: 0 186 393 1140 1266
Size: 186 207 747 126
Fragments arranged by size:
747 207 186 126
BtgI C'CryG_G
Cuts at: 0 272 723 1266
Size: 272 451 543
Fragments arranged by size:
543 451 272
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 165 321 372 1266
Size: 165 156 51 894
Fragments arranged by size:
894 165 156 51
DraIII CAC_nnn'GTG
Cuts at: 0 891 1266
Size: 891 375
DrdI GACnn_nn'nnGTC
Cuts at: 0 94 1266
Size: 94 1172
EaeI y'GGCC_r
Cuts at: 0 209 275 1266
Size: 209 66 991
Fragments arranged by size:
991 209 66
EarI CTCTTCn'nnn_
Cuts at: 0 748 1266
Size: 748 518
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 807 889 1266
Size: 807 82 377
Fragments arranged by size:
807 377 82
HaeII r_GCGC'y
Cuts at: 0 195 863 1148 1266
Size: 195 668 285 118
Fragments arranged by size:
668 285 195 118
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 102 134 1266
Size: 102 32 1132
Fragments arranged by size:
1132 102 32
KasI G'GCGC_C
Cuts at: 0 859 1144 1266
Size: 859 285 122
Fragments arranged by size:
859 285 122
MfeI C'AATT_G
Cuts at: 0 1155 1266
Size: 1155 111
MluI A'CGCG_T
Cuts at: 0 1226 1266
Size: 1226 40
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 1196 1266
Size: 110 1086 70
Fragments arranged by size:
1086 110 70
MscI TGG'CCA
Cuts at: 0 211 1266
Size: 211 1055
MslI CAynn'nnrTG
Cuts at: 0 1048 1266
Size: 1048 218
MspA1I CmG'CkG
Cuts at: 0 460 621 1121 1266
Size: 460 161 500 145
Fragments arranged by size:
500 460 161 145
NarI GG'CG_CC
Cuts at: 0 860 1145 1266
Size: 860 285 121
Fragments arranged by size:
860 285 121
NcoI C'CATG_G
Cuts at: 0 272 723 1266
Size: 272 451 543
Fragments arranged by size:
543 451 272
NspI r_CATG'y
Cuts at: 0 560 1131 1266
Size: 560 571 135
Fragments arranged by size:
571 560 135
PstI C_TGCA'G
Cuts at: 0 203 1266
Size: 203 1063
PvuII CAG'CTG
Cuts at: 0 1121 1266
Size: 1121 145
SfcI C'TryA_G
Cuts at: 0 199 1266
Size: 199 1067
SfoI GGC'GCC
Cuts at: 0 861 1146 1266
Size: 861 285 120
Fragments arranged by size:
861 285 120
SphI G_CATG'C
Cuts at: 0 560 1266
Size: 560 706
StyI C'CwwG_G
Cuts at: 0 185 272 723 1266
Size: 185 87 451 543
Fragments arranged by size:
543 451 185 87
Enzymes that do cut:
AfeI AflIII AlwNI ApaLI AscI AvaI BanI BbeI
BbsI BclI BlpI Bme1580I BmgBI BmrI BpmI BsaI
BsaAI BsaHI BsaWI BseYI BsgI BsiHKAI Bsp1286I BspMI
BsrBI BsrDI BsrFI BssHII BstAPI BstEII BstXI BtgI
BtgZI DraIII DrdI EaeI EarI Eco57MI HaeII Hin4I
KasI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NspI PstI PvuII SfcI SfoI SphI StyI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AgeI
AhdI AleI AlfI AloI ApaI ApoI AseI AsiSI
AvrII BaeI BamHI BanII BbvCI BcgI BciVI BfrBI
BglI BglII BmtI BplI Bpu10I BpuEI BsaBI BsaXI
BseRI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrGI
BssSI BstBI BstYI BstZ17I Bsu36I BtsI ClaI CspCI
DraI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KpnI
NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI
StuI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI