(Circular) (Six-base) MAPSORT of: pFN21AB9856.seq Check: 7915 from: 1 to: 5875 pFN21AB9856 5875 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3685 278 Size: 53 83 186 936 2149 2468 Fragments arranged by size: 2468 2149 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2634 3218 1937 Size: 697 584 4594 Fragments arranged by size: 4594 697 584 Acc65I G'GTAC_C Cuts at: 3196 3196 Size: 5875 AclI AA'CG_TT Cuts at: 1563 4002 4375 1563 Size: 2439 373 3063 Fragments arranged by size: 3063 2439 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3938 5322 5590 1632 Size: 2306 1384 268 1917 Fragments arranged by size: 2306 1917 1384 268 AfeI AGC'GCT Cuts at: 2753 2753 Size: 5875 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5678 AflIII A'CryG_T Cuts at: 1203 4774 5751 1203 Size: 3571 977 1327 Fragments arranged by size: 3571 1327 977 AgeI A'CCGG_T Cuts at: 4706 4706 Size: 5875 AhdI GACnn_n'nnGTC Cuts at: 4604 4604 Size: 5875 AleI CACnn'nnGTG Cuts at: 1166 2424 1166 Size: 1258 4617 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5875 AlwNI CAG_nnn'CTG Cuts at: 3618 5190 3618 Size: 1572 4303 ApaLI G'TGCA_C Cuts at: 3166 3932 5088 3166 Size: 766 1156 3953 Fragments arranged by size: 3953 1156 766 ApoI r'AATT_y Cuts at: 1493 3184 3410 3562 5671 1493 Size: 1691 226 152 2109 1697 Fragments arranged by size: 2109 1697 1691 226 152 AseI AT'TA_AT Cuts at: 160 1032 4429 160 Size: 872 3397 1606 Fragments arranged by size: 3397 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5875 AvaI C'yCGr_G Cuts at: 1870 1943 2760 2786 3200 1870 Size: 73 817 26 414 4545 Fragments arranged by size: 4545 817 414 73 26 AvrII C'CTAG_G Cuts at: 2950 2950 Size: 5875 BamHI G'GATC_C Cuts at: 3205 3572 3205 Size: 367 5508 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3196 4651 618 Size: 325 217 627 1409 1455 1842 Fragments arranged by size: 1842 1455 1409 627 325 217 BanII G_rGCy'C Cuts at: 1391 2051 2767 3194 1391 Size: 660 716 427 4072 Fragments arranged by size: 4072 716 660 427 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2521 928 Size: 971 622 4282 Fragments arranged by size: 4282 971 622 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4066 4100 1903 Size: 34 2129 34 3678 Fragments arranged by size: 3678 2129 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3767 4977 3767 Size: 1210 4665 BclI T'GATC_A Cuts at: 1548 1548 Size: 5875 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2276 4486 136 Size: 107 122 71 631 605 604 2210 1525 Fragments arranged by size: 2210 1525 631 605 604 122 107 71 BglII A'GATC_T Cuts at: 5870 5870 Size: 5875 BlpI GC'TnA_GC Cuts at: 3292 3292 Size: 5875 Bme1580I G_kGCm'C Cuts at: 1790 2838 3170 3936 5092 1790 Size: 1048 332 766 1156 2573 Fragments arranged by size: 2573 1156 1048 766 332 BmgBI CAC'GTC Cuts at: 1318 2796 2833 1318 Size: 1478 37 4360 Fragments arranged by size: 4360 1478 37 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2363 2458 3606 Size: 416 53 83 399 24 946 95 1148 Cuts at: 3606 4564 442 Size: 958 1753 Fragments arranged by size: 1753 1148 958 946 416 399 95 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5875 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4535 1239 Size: 3296 2579 Bpu10I CC'TnA_GC Cuts at: 2574 2574 Size: 5875 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2052 4000 4865 5163 2052 Size: 1948 865 298 2764 Fragments arranged by size: 2764 1948 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2048 4538 882 Size: 617 549 2490 2219 Fragments arranged by size: 2490 2219 617 549 BsaAI yAC'GTr Cuts at: 493 1204 2701 3666 493 Size: 711 1497 965 2702 Fragments arranged by size: 2702 1497 965 711 BsaBI GATnn'nnATC Cuts at: 2315 3017 5869 2315 Size: 702 2852 2321 Fragments arranged by size: 2852 2321 702 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2463 2566 2732 2892 Size: 53 83 186 936 930 103 166 160 Cuts at: 2892 3682 4064 4699 275 Size: 790 382 635 1451 Fragments arranged by size: 1451 936 930 790 635 382 186 166 160 103 83 53 BsaWI w'CCGG_w Cuts at: 2229 2846 4308 4706 4980 5127 5538 2229 Size: 617 1462 398 274 147 411 2566 Fragments arranged by size: 2566 1462 617 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2114 2120 2144 2150 1178 Size: 30 165 30 711 6 24 6 4903 Fragments arranged by size: 4903 711 165 30 30 24 6 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2600 2663 1186 Size: 1414 63 4398 Fragments arranged by size: 4398 1414 63 BseYI C'CCAG_C Cuts at: 2766 2837 3073 5078 5574 2766 Size: 71 236 2005 496 3067 Fragments arranged by size: 3067 2005 496 236 71 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2228 2402 1163 Size: 573 492 174 4636 Fragments arranged by size: 4636 573 492 174 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2344 3325 4086 5114 1540 Size: 275 186 343 981 761 1028 2301 Fragments arranged by size: 2301 1028 981 761 343 275 186 BsiHKAI G_wGCw'C Cuts at: 2023 3170 3936 4021 5092 2023 Size: 1147 766 85 1071 2806 Fragments arranged by size: 2806 1147 1071 766 85 BsmI GAATG_Cn' Cuts at: 2603 3140 3386 3479 2603 Size: 537 246 93 4999 Fragments arranged by size: 4999 537 246 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2023 2051 2767 2838 3170 3194 3936 Size: 399 233 28 716 71 332 24 742 Cuts at: 3936 4021 5092 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 716 399 332 233 85 71 28 24 BspEI T'CCGG_A Cuts at: 2846 5538 2846 Size: 2692 3183 BspHI T'CATG_A Cuts at: 2508 3764 5494 2508 Size: 1256 1730 2889 Fragments arranged by size: 2889 1730 1256 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2298 3230 844 Size: 1454 932 3489 Fragments arranged by size: 3489 1454 932 BsrBI CCG'CTC Cuts at: 1394 1823 2712 3762 1394 Size: 429 889 1050 3507 Fragments arranged by size: 3507 1050 889 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4370 4544 65 Size: 1183 3122 174 1396 Fragments arranged by size: 3122 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2339 2744 4519 4706 1700 Size: 110 529 405 1775 187 2869 Fragments arranged by size: 2869 1775 529 405 187 110 BsrGI T'GTAC_A Cuts at: 96 2328 96 Size: 2232 3643 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5875 BssSI C'ACGA_G Cuts at: 3935 4947 3935 Size: 1012 4863 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5875 BstBI TT'CG_AA Cuts at: 4679 4679 Size: 5875 BstEII G'GTnAC_C Cuts at: 1184 2281 2803 1184 Size: 1097 522 4256 Fragments arranged by size: 4256 1097 522 BstYI r'GATC_y Cuts at: 1972 2849 3205 3572 3957 3974 5426 5512 5524 Size: 877 356 367 385 17 1452 86 12 Cuts at: 5524 5870 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 877 385 367 356 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2043 2466 2640 513 Size: 552 939 39 423 174 3748 Fragments arranged by size: 3748 939 552 423 174 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2416 2662 487 Size: 683 56 1190 246 3700 Fragments arranged by size: 3700 1190 683 246 56 BtsI GCAGTG_nn' Cuts at: 2403 3391 4185 4205 2403 Size: 988 794 20 4073 Fragments arranged by size: 4073 988 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5875 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5840 DraI TTT'AAA Cuts at: 3133 3179 3133 Size: 46 5829 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4882 809 Size: 507 3566 1802 Fragments arranged by size: 3566 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2187 2341 2689 Size: 54 1425 50 275 18 357 154 348 Cuts at: 2689 2919 3322 4211 5840 8 Size: 230 403 889 1629 43 Fragments arranged by size: 1629 1425 889 403 357 348 275 230 154 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2341 3322 1537 Size: 275 529 981 4090 Fragments arranged by size: 4090 981 529 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2577 3805 1353 Size: 1224 1228 3423 Fragments arranged by size: 3423 1228 1224 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4462 4836 4982 387 Size: 1064 138 2873 374 146 1280 Fragments arranged by size: 2873 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3938 4535 5322 5590 1239 Size: 393 2306 597 787 268 1524 Fragments arranged by size: 2306 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 3184 3562 3184 Size: 378 5497 FspI TGC'GCA Cuts at: 4381 4381 Size: 5875 HaeII r_GCGC'y Cuts at: 1678 2109 2177 2755 5022 1678 Size: 431 68 578 2267 2531 Fragments arranged by size: 2531 2267 578 431 68 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2120 2152 2257 2289 2576 Size: 32 135 32 548 32 105 32 287 Cuts at: 2576 2608 4516 4548 4590 4622 1373 Size: 32 1908 32 42 32 2626 Fragments arranged by size: 2626 1908 548 287 135 105 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3219 3465 669 Size: 988 281 1281 246 3079 Fragments arranged by size: 3079 1281 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5875 HpaI GTT'AAC Cuts at: 3465 3465 Size: 5875 KpnI G_GTAC'C Cuts at: 3200 3200 Size: 5875 MfeI C'AATT_G Cuts at: 3474 3474 Size: 5875 MluI A'CGCG_T Cuts at: 5751 5751 Size: 5875 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2367 2574 2913 4989 5173 1305 Size: 1062 207 339 2076 184 2007 Fragments arranged by size: 2076 2007 1062 339 207 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2189 10 Size: 54 1425 343 357 3696 Fragments arranged by size: 3696 1425 357 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2306 2424 3833 4192 4351 518 Size: 648 1140 118 1409 359 159 2042 Fragments arranged by size: 2042 1409 1140 648 359 159 118 MspA1I CmG'CkG Cuts at: 1606 2045 2346 2770 3291 3968 5116 5361 1606 Size: 439 301 424 521 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 521 439 424 301 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4384 Fragments arranged by size: 4384 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5875 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5875 NotI GC'GGCC_GC Cuts at: 3322 3322 Size: 5875 NspI r_CATG'y Cuts at: 1132 1229 3233 4778 1132 Size: 97 2004 1545 2229 Fragments arranged by size: 2229 2004 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5875 PciI A'CATG_T Cuts at: 4774 4774 Size: 5875 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5875 PfoI T'CCnGG_A Cuts at: 2714 2714 Size: 5875 PmeI GTTT'AAAC Cuts at: 3179 3179 Size: 5875 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3944 3976 5457 5489 3944 Size: 32 1481 32 4330 Fragments arranged by size: 4330 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5875 PsiI TTA'TAA Cuts at: 3445 3445 Size: 5875 PstI C_TGCA'G Cuts at: 830 2105 3227 830 Size: 1275 1122 3478 Fragments arranged by size: 3478 1275 1122 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5875 PvuII CAG'CTG Cuts at: 2770 2770 Size: 5875 SacII CC_GC'GG Cuts at: 2046 2046 Size: 5875 SalI G'TCGA_C Cuts at: 1936 3217 1936 Size: 1281 4594 SbfI CC_TGCA'GG Cuts at: 3227 3227 Size: 5875 ScaI AGT'ACT Cuts at: 2154 2474 4123 5835 2154 Size: 320 1649 1712 2194 Fragments arranged by size: 2194 1712 1649 320 SfcI C'TryA_G Cuts at: 826 1045 2101 3223 4358 5039 5230 826 Size: 219 1056 1122 1135 681 191 1471 Fragments arranged by size: 1471 1135 1122 1056 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5875 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5875 SmaI CCC'GGG Cuts at: 1872 2788 3202 1872 Size: 916 414 4545 Fragments arranged by size: 4545 916 414 SmlI C'TyrA_G Cuts at: 820 1017 1943 2067 3979 4880 5142 820 Size: 197 926 124 1912 901 262 1553 Fragments arranged by size: 1912 1553 926 901 262 197 124 SnaBI TAC'GTA Cuts at: 493 2701 3666 493 Size: 2208 965 2702 Fragments arranged by size: 2702 2208 965 SpeI A'CTAG_T Cuts at: 152 152 Size: 5875 SphI G_CATG'C Cuts at: 1132 3233 1132 Size: 2101 3774 SspI AAT'ATT Cuts at: 5 52 3799 5 Size: 47 3747 2081 Fragments arranged by size: 3747 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5875 StyI C'CwwG_G Cuts at: 513 1065 2004 2109 2184 2950 3314 513 Size: 552 939 105 75 766 364 3074 Fragments arranged by size: 3074 939 766 552 364 105 75 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2636 2791 3902 3919 4072 4257 4702 Size: 1255 1056 155 1111 17 153 185 445 Cuts at: 4702 325 Size: 1498 Fragments arranged by size: 1498 1255 1111 1056 445 185 155 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2152 2328 2472 Size: 274 80 33 51 1443 175 176 144 Cuts at: 2472 4121 5833 96 Size: 1649 1712 138 Fragments arranged by size: 1712 1649 1443 274 176 175 144 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2841 2873 5835 5867 2841 Size: 32 2962 32 2849 Fragments arranged by size: 2962 2849 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5875 XbaI T'CTAG_A Cuts at: 3211 3211 Size: 5875 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5875 XmaI C'CCGG_G Cuts at: 1870 2786 3200 1870 Size: 916 414 4545 Fragments arranged by size: 4545 916 414 XmnI GAAnn'nnTTC Cuts at: 4004 4004 Size: 5875 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3683 276 Size: 53 83 186 936 2149 2468 Fragments arranged by size: 2468 2149 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI