Restriction map for
pFN21AB9856
(Linear) (Six-base) MAPSORT of: KIBB9856.seq Check: 3515 from: 1 to: 1170
KIBB9856.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AccI GT'mk_AC
Cuts at: 0 629 1170
Size: 629 541
AfeI AGC'GCT
Cuts at: 0 748 1170
Size: 748 422
AleI CACnn'nnGTG
Cuts at: 0 419 1170
Size: 419 751
ApaLI G'TGCA_C
Cuts at: 0 1161 1170
Size: 1161 9
AvaI C'yCGr_G
Cuts at: 0 755 781 1170
Size: 755 26 389
Fragments arranged by size:
755 389 26
AvrII C'CTAG_G
Cuts at: 0 945 1170
Size: 945 225
BanII G_rGCy'C
Cuts at: 0 46 762 1170
Size: 46 716 408
Fragments arranged by size:
716 408 46
BbsI GAAGACnn'nnnn_
Cuts at: 0 516 1170
Size: 516 654
BglI GCCn_nnn'nGGC
Cuts at: 0 271 1170
Size: 271 899
Bme1580I G_kGCm'C
Cuts at: 0 833 1165 1170
Size: 833 332 5
Fragments arranged by size:
833 332 5
BmgBI CAC'GTC
Cuts at: 0 791 828 1170
Size: 791 37 342
Fragments arranged by size:
791 342 37
BmrI ACTGGGnnnn_n'
Cuts at: 0 358 453 1170
Size: 358 95 717
Fragments arranged by size:
717 358 95
Bpu10I CC'TnA_GC
Cuts at: 0 569 1170
Size: 569 601
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 47 1170
Size: 47 1123
BsaI GGTCTCn'nnnn_
Cuts at: 0 43 1170
Size: 43 1127
BsaAI yAC'GTr
Cuts at: 0 696 1170
Size: 696 474
BsaBI GATnn'nnATC
Cuts at: 0 310 1012 1170
Size: 310 702 158
Fragments arranged by size:
702 310 158
BsaHI Gr'CG_yC
Cuts at: 0 458 561 727 887 1170
Size: 458 103 166 160 283
Fragments arranged by size:
458 283 166 160 103
BsaWI w'CCGG_w
Cuts at: 0 224 841 1170
Size: 224 617 329
Fragments arranged by size:
617 329 224
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 109 115 139 145 1170
Size: 109 6 24 6 1025
Fragments arranged by size:
1025 109 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 595 658 1170
Size: 595 63 512
Fragments arranged by size:
595 512 63
BseYI C'CCAG_C
Cuts at: 0 761 832 1068 1170
Size: 761 71 236 102
Fragments arranged by size:
761 236 102 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 397 1170
Size: 223 174 773
Fragments arranged by size:
773 223 174
BsiEI CG_ry'CG
Cuts at: 0 339 1170
Size: 339 831
BsiHKAI G_wGCw'C
Cuts at: 0 18 1165 1170
Size: 18 1147 5
Fragments arranged by size:
1147 18 5
BsmI GAATG_Cn'
Cuts at: 0 598 1135 1170
Size: 598 537 35
Fragments arranged by size:
598 537 35
Bsp1286I G_dGCh'C
Cuts at: 0 18 46 762 833 1165 1170
Size: 18 28 716 71 332 5
Fragments arranged by size:
716 332 71 28 18 5
BspEI T'CCGG_A
Cuts at: 0 841 1170
Size: 841 329
BspHI T'CATG_A
Cuts at: 0 503 1170
Size: 503 667
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 293 1170
Size: 293 877
BsrBI CCG'CTC
Cuts at: 0 707 1170
Size: 707 463
BsrFI r'CCGG_y
Cuts at: 0 334 739 1170
Size: 334 405 431
Fragments arranged by size:
431 405 334
BsrGI T'GTAC_A
Cuts at: 0 323 1170
Size: 323 847
BstEII G'GTnAC_C
Cuts at: 0 276 798 1170
Size: 276 522 372
Fragments arranged by size:
522 372 276
BstYI r'GATC_y
Cuts at: 0 844 1170
Size: 844 326
BtgI C'CryG_G
Cuts at: 0 38 461 635 1170
Size: 38 423 174 535
Fragments arranged by size:
535 423 174 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 411 657 1170
Size: 411 246 513
Fragments arranged by size:
513 411 246
BtsI GCAGTG_nn'
Cuts at: 0 398 1170
Size: 398 772
DraI TTT'AAA
Cuts at: 0 1128 1170
Size: 1128 42
EaeI y'GGCC_r
Cuts at: 0 182 336 684 914 1170
Size: 182 154 348 230 256
Fragments arranged by size:
348 256 230 182 154
EagI C'GGCC_G
Cuts at: 0 336 1170
Size: 336 834
EarI CTCTTCn'nnn_
Cuts at: 0 572 1170
Size: 572 598
HaeII r_GCGC'y
Cuts at: 0 104 172 750 1170
Size: 104 68 578 420
Fragments arranged by size:
578 420 104 68
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 115 147 252 284 571 603 1170
Size: 115 32 105 32 287 32 567
Fragments arranged by size:
567 287 115 105 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 362 569 908 1170
Size: 362 207 339 262
Fragments arranged by size:
362 339 262 207
MscI TGG'CCA
Cuts at: 0 184 1170
Size: 184 986
MslI CAynn'nnrTG
Cuts at: 0 301 419 1170
Size: 301 118 751
Fragments arranged by size:
751 301 118
MspA1I CmG'CkG
Cuts at: 0 40 341 765 1170
Size: 40 301 424 405
Fragments arranged by size:
424 405 301 40
PfoI T'CCnGG_A
Cuts at: 0 709 1170
Size: 709 461
PstI C_TGCA'G
Cuts at: 0 100 1170
Size: 100 1070
PvuII CAG'CTG
Cuts at: 0 765 1170
Size: 765 405
SacII CC_GC'GG
Cuts at: 0 41 1170
Size: 41 1129
ScaI AGT'ACT
Cuts at: 0 149 469 1170
Size: 149 320 701
Fragments arranged by size:
701 320 149
SfcI C'TryA_G
Cuts at: 0 96 1170
Size: 96 1074
SmaI CCC'GGG
Cuts at: 0 783 1170
Size: 783 387
SmlI C'TyrA_G
Cuts at: 0 62 1170
Size: 62 1108
SnaBI TAC'GTA
Cuts at: 0 696 1170
Size: 696 474
StyI C'CwwG_G
Cuts at: 0 104 179 945 1170
Size: 104 75 766 225
Fragments arranged by size:
766 225 104 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 631 786 1170
Size: 631 155 384
Fragments arranged by size:
631 384 155
TatI w'GTAC_w
Cuts at: 0 147 323 467 1170
Size: 147 176 144 703
Fragments arranged by size:
703 176 147 144
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 836 868 1170
Size: 836 32 302
Fragments arranged by size:
836 302 32
XmaI C'CCGG_G
Cuts at: 0 781 1170
Size: 781 389
Enzymes that do cut:
AccI AfeI AleI ApaLI AvaI AvrII BanII BbsI
BglI Bme1580I BmgBI BmrI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI
BsrGI BstEII BstYI BtgI BtgZI BtsI DraI EaeI
EagI EarI HaeII Hin4I MmeI MscI MslI MspA1I
PfoI PstI PvuII SacII ScaI SfcI SmaI SmlI
SnaBI StyI TaqII TatI TstI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApoI AscI AseI
AsiSI BaeI BamHI BanI BbeI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmtI BplI BpmI BsiWI
BsmBI BsrDI BssHII BssSI BstAPI BstBI BstXI BstZ17I
Bsu36I ClaI CspCI DraIII DrdI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SalI SanDI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SpeI SphI SrfI SspI StuI SwaI
Tth111I XbaI XcmI XhoI XmnI ZraI