(Circular) (Six-base) MAPSORT of: pFN21AB9854.seq Check: 8596 from: 1 to: 5872 pFN21AB9854 5872 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3682 278 Size: 53 83 186 936 2146 2468 Fragments arranged by size: 2468 2146 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2326 3215 1937 Size: 389 889 4594 Fragments arranged by size: 4594 889 389 Acc65I G'GTAC_C Cuts at: 3084 3193 3084 Size: 109 5763 AclI AA'CG_TT Cuts at: 1563 2782 3999 4372 1563 Size: 1219 1217 373 3063 Fragments arranged by size: 3063 1219 1217 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2709 2735 3935 5319 5587 1632 Size: 1077 26 1200 1384 268 1917 Fragments arranged by size: 1917 1384 1200 1077 268 26 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5675 AflIII A'CryG_T Cuts at: 1203 4771 5748 1203 Size: 3568 977 1327 Fragments arranged by size: 3568 1327 977 AgeI A'CCGG_T Cuts at: 4703 4703 Size: 5872 AhdI GACnn_n'nnGTC Cuts at: 4601 4601 Size: 5872 AleI CACnn'nnGTG Cuts at: 1166 3150 1166 Size: 1984 3888 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5872 AlwNI CAG_nnn'CTG Cuts at: 3615 5187 3615 Size: 1572 4300 ApaLI G'TGCA_C Cuts at: 3929 5085 3929 Size: 1156 4716 ApoI r'AATT_y Cuts at: 1493 2098 2291 2470 2483 2513 3181 3407 3559 Size: 605 193 179 13 30 668 226 152 Cuts at: 3559 5668 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 668 605 226 193 179 152 30 13 AseI AT'TA_AT Cuts at: 160 1032 4426 160 Size: 872 3394 1606 Fragments arranged by size: 3394 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5872 AvaI C'yCGr_G Cuts at: 1870 1943 3197 1870 Size: 73 1254 4545 Fragments arranged by size: 4545 1254 73 BamHI G'GATC_C Cuts at: 2545 3202 3569 2545 Size: 657 367 4848 Fragments arranged by size: 4848 657 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3084 3193 4648 618 Size: 325 217 627 1297 109 1455 1842 Fragments arranged by size: 1842 1455 1297 627 325 217 109 BanII G_rGCy'C Cuts at: 1391 3191 1391 Size: 1800 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2965 928 Size: 971 1066 3835 Fragments arranged by size: 3835 1066 971 BbvCI CC'TCA_GC Cuts at: 3075 3075 Size: 5872 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4063 4097 1903 Size: 34 2126 34 3678 Fragments arranged by size: 3678 2126 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3764 4974 3764 Size: 1210 4662 BclI T'GATC_A Cuts at: 1548 2976 1548 Size: 1428 4444 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4483 136 Size: 107 122 71 631 605 2811 1525 Fragments arranged by size: 2811 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5867 5867 Size: 5872 BlpI GC'TnA_GC Cuts at: 3289 3289 Size: 5872 Bme1580I G_kGCm'C Cuts at: 1790 3933 5089 1790 Size: 2143 1156 2573 Fragments arranged by size: 2573 2143 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5872 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2472 3603 4561 Size: 416 53 83 399 24 1055 1131 958 Cuts at: 4561 442 Size: 1753 Fragments arranged by size: 1753 1131 1055 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5872 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4532 1239 Size: 3293 2579 Bpu10I CC'TnA_GC Cuts at: 3075 3105 3075 Size: 30 5842 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3997 4862 5160 3997 Size: 865 298 4709 Fragments arranged by size: 4709 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4535 882 Size: 617 3036 2219 Fragments arranged by size: 3036 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2395 3663 493 Size: 711 1191 1268 2702 Fragments arranged by size: 2702 1268 1191 711 BsaBI GATnn'nnATC Cuts at: 5866 5866 Size: 5872 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3679 4061 4696 275 Size: 53 83 186 936 2146 382 635 1451 Fragments arranged by size: 2146 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4305 4703 4977 5124 5535 4305 Size: 398 274 147 411 4642 Fragments arranged by size: 4642 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2952 2982 1178 Size: 30 165 30 1549 30 4068 Fragments arranged by size: 4068 1549 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5872 BseYI C'CCAG_C Cuts at: 5075 5571 5075 Size: 496 5376 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3128 1163 Size: 573 1392 3907 Fragments arranged by size: 3907 1392 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3322 4083 5111 1540 Size: 275 186 1321 761 1028 2301 Fragments arranged by size: 2301 1321 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2795 3933 4018 5089 2795 Size: 1138 85 1071 3578 Fragments arranged by size: 3578 1138 1071 85 BsmI GAATG_Cn' Cuts at: 3383 3476 3383 Size: 93 5779 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2795 3191 3933 4018 5089 1391 Size: 399 1005 396 742 85 1071 2174 Fragments arranged by size: 2174 1071 1005 742 399 396 85 BspEI T'CCGG_A Cuts at: 5535 5535 Size: 5872 BspHI T'CATG_A Cuts at: 2379 2943 3761 5491 2379 Size: 564 818 1730 2760 Fragments arranged by size: 2760 1730 818 564 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3227 844 Size: 2383 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3759 1394 Size: 429 1936 3507 Fragments arranged by size: 3507 1936 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2425 2652 4367 4541 65 Size: 1183 1177 227 1715 174 1396 Fragments arranged by size: 1715 1396 1183 1177 227 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4516 4703 1700 Size: 110 2706 187 2869 Fragments arranged by size: 2869 2706 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5872 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5872 BssSI C'ACGA_G Cuts at: 3932 4944 3932 Size: 1012 4860 BstAPI GCAn_nnn'nTGC Cuts at: 794 2937 794 Size: 2143 3729 BstBI TT'CG_AA Cuts at: 4676 4676 Size: 5872 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5872 BstYI r'GATC_y Cuts at: 1972 2248 2545 3202 3569 3954 3971 5423 5509 Size: 276 297 657 367 385 17 1452 86 Cuts at: 5509 5521 5867 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 657 385 367 346 297 276 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5320 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5133 Fragments arranged by size: 5133 683 56 BtsI GCAGTG_nn' Cuts at: 3140 3388 4182 4202 3140 Size: 248 794 20 4810 Fragments arranged by size: 4810 794 248 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5872 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5837 DraI TTT'AAA Cuts at: 3176 3176 Size: 5872 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4879 809 Size: 507 3563 1802 Fragments arranged by size: 3563 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3319 4208 5837 Size: 54 1425 50 275 18 1489 889 1629 Cuts at: 5837 8 Size: 43 Fragments arranged by size: 1629 1489 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3319 1537 Size: 275 1507 4090 Fragments arranged by size: 4090 1507 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2685 3802 1353 Size: 1332 1117 3423 Fragments arranged by size: 3423 1332 1117 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4459 4833 4979 387 Size: 1064 138 2870 374 146 1280 Fragments arranged by size: 2870 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2709 2735 3935 4532 5319 5587 1239 Size: 393 1077 26 1200 597 787 268 1524 Fragments arranged by size: 1524 1200 1077 787 597 393 268 26 EcoRI G'AATT_C Cuts at: 3181 3559 3181 Size: 378 5494 EcoRV GAT'ATC Cuts at: 2377 2377 Size: 5872 FspI TGC'GCA Cuts at: 4378 4378 Size: 5872 HaeII r_GCGC'y Cuts at: 1678 5019 1678 Size: 3341 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2952 2984 4513 4545 4587 Size: 32 135 32 1380 32 1529 32 42 Cuts at: 4587 4619 1373 Size: 32 2626 Fragments arranged by size: 2626 1529 1380 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3216 3462 669 Size: 988 281 1278 246 3079 Fragments arranged by size: 3079 1278 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5872 HpaI GTT'AAC Cuts at: 3462 3462 Size: 5872 KpnI G_GTAC'C Cuts at: 3088 3197 3088 Size: 109 5763 MfeI C'AATT_G Cuts at: 3471 3471 Size: 5872 MluI A'CGCG_T Cuts at: 5748 5748 Size: 5872 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2414 4986 5170 1305 Size: 1109 2572 184 2007 Fragments arranged by size: 2572 2007 1109 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4050 Fragments arranged by size: 4050 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2984 3150 3830 4189 4348 518 Size: 648 1818 166 680 359 159 2042 Fragments arranged by size: 2042 1818 680 648 359 166 159 MspA1I CmG'CkG Cuts at: 1606 3074 3288 3965 5113 5358 1606 Size: 1468 214 677 1148 245 2120 Fragments arranged by size: 2120 1468 1148 677 245 214 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5320 NdeI CA'TA_TG Cuts at: 387 2672 387 Size: 2285 3587 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5872 NotI GC'GGCC_GC Cuts at: 3319 3319 Size: 5872 NspI r_CATG'y Cuts at: 1132 1229 2626 3230 4775 1132 Size: 97 1397 604 1545 2229 Fragments arranged by size: 2229 1545 1397 604 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5872 PciI A'CATG_T Cuts at: 4771 4771 Size: 5872 PflMI CCAn_nnn'nTGG Cuts at: 1108 2087 1108 Size: 979 4893 PfoI T'CCnGG_A Cuts at: 2637 2637 Size: 5872 PmeI GTTT'AAAC Cuts at: 3176 3176 Size: 5872 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3941 3973 5454 5486 3941 Size: 32 1481 32 4327 Fragments arranged by size: 4327 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5872 PsiI TTA'TAA Cuts at: 2048 3442 2048 Size: 1394 4478 PstI C_TGCA'G Cuts at: 830 2726 3224 830 Size: 1896 498 3478 Fragments arranged by size: 3478 1896 498 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5872 PvuII CAG'CTG Cuts at: 3074 3074 Size: 5872 SalI G'TCGA_C Cuts at: 1936 3214 1936 Size: 1278 4594 SbfI CC_TGCA'GG Cuts at: 3224 3224 Size: 5872 ScaI AGT'ACT Cuts at: 2720 3000 4120 5832 2720 Size: 280 1120 1712 2760 Fragments arranged by size: 2760 1712 1120 280 SfcI C'TryA_G Cuts at: 826 1045 2563 2722 3220 4355 5036 5227 826 Size: 219 1518 159 498 1135 681 191 1471 Fragments arranged by size: 1518 1471 1135 681 498 219 191 159 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5872 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5872 SmaI CCC'GGG Cuts at: 1872 3199 1872 Size: 1327 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3976 4877 5139 820 Size: 197 926 2033 901 262 1553 Fragments arranged by size: 2033 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2395 3663 493 Size: 1902 1268 2702 Fragments arranged by size: 2702 1902 1268 SpeI A'CTAG_T Cuts at: 152 152 Size: 5872 SphI G_CATG'C Cuts at: 1132 3230 1132 Size: 2098 3774 SspI AAT'ATT Cuts at: 5 52 3016 3796 5 Size: 47 2964 780 2081 Fragments arranged by size: 2964 2081 780 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5872 StyI C'CwwG_G Cuts at: 513 1065 3311 513 Size: 552 2246 3074 Fragments arranged by size: 3074 2246 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3153 3899 3916 4069 4254 4699 325 Size: 1255 1573 746 17 153 185 445 1498 Fragments arranged by size: 1573 1498 1255 746 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2718 2998 4118 Size: 274 80 33 51 1443 741 280 1120 Cuts at: 4118 5830 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1120 741 280 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5832 5864 5832 Size: 32 5840 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5872 XbaI T'CTAG_A Cuts at: 2317 2795 3208 2317 Size: 478 413 4981 Fragments arranged by size: 4981 478 413 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5872 XmaI C'CCGG_G Cuts at: 1870 3197 1870 Size: 1327 4545 XmnI GAAnn'nnTTC Cuts at: 2076 2292 4001 2076 Size: 216 1709 3947 Fragments arranged by size: 3947 1709 216 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3680 276 Size: 53 83 186 936 2146 2468 Fragments arranged by size: 2468 2146 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI