Restriction map for
pFN21AB9852
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# NO CUT ENZYMES ( 157 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspHI
AspI AvaI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BclI BcnI BfrI
BglI BglII BlnI BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstUI BstZ17I Cfr10I
ClaI CpoI DraI DraIII DrdI
EaeI EagI EclXI Eco47III Eco52I
EcoO65I EcoRV EcoT22I FbaI FokI
FseI FspI HapII HgaI Hin1I
HincII HindII HindIII HpaI HpaII
KasI KpnI KspI MaeII MamI
MfeI MluI MluNI MroI MscI
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcsI 1026 GAATTC [GA]^AATT[CT]
AhdI 775 GACTGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 17 CCTTAGG CC^T[ACGT]AGG
ApoI 1026 GAATTC [GA]^AATT[CT]
Asp700I 878 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 775 GACTGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII 762 GAGCCC G[GA]GC[CT]^C
BciVI 473 GTATCC GTATCC(6/5)
BlpI 759 GCTGAGC GC^T[ACGT]AGC
BmyI 762 GAGCCC G[AGT]GC[ACT]^C
Bpu1102I 759 GCTGAGC GC^T[ACGT]AGC
BsaI 782 GGTCTC GGTCTC(1/5)
BsgI 941 GTGCAG GTGCAG(16/14)
Bsp1286I 762 GAGCCC G[AGT]GC[ACT]^C
BsrBI 824 CCGCTC CCGCTC(-3/-3)
BstXI 348 CCAAACTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 567 AGATCC [GA]^GATC[CT]
Bsu36I 17 CCTTAGG CC^T[ACGT]AGG
CelII 759 GCTGAGC GC^T[ACGT]AGC
DraII 233 GGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 608 CCACGG C^C[GA][CT]GG
Eam1105I 775 GACTGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 17 CCTTAGG CC^T[ACGT]AGG
EcoNI 30 CCTTGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 233 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 1026 GAATTC G^AATTC
EcoT14I 650 CCTTGG C^C[AT][AT]GG
FauI 967 GCGGG CCCGC(4/6)
HaeII 13 AGCGCC [GA]GCGC^[CT]
MflI 567 AGATCC [GA]^GATC[CT]
MspA1I 116 CAGCGG C[AC]G^C[GT]G
NlaIV 233 GGGTCC GG[ACGT]^[ACGT]CC
NspI 371 ACATGC [GA]CATG^[CT]
PleI 124 GAGTC GAGTC(4/5)
PpuMI 233 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 238 CTGCAG CTGCA^G
SapI 418 GAAGAGC GCTCTTC(1/4)
ScaI 81 AGTACT AGT^ACT
SexAI 174 ACCTGGT A^CC[AT]GGT
SfaNI 297 GATGC GCATC(5/9)
SfcI 238 CTGCAG C^T[GA][CT]AG
StyI 650 CCTTGG C^C[AT][AT]GG
TaqI 954 TCGA T^CGA
Tsp45I 5 GTGAC ^GT[GC]AC
TthHB8I 954 TCGA T^CGA
XcmI 741 CCATGCGACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 567 AGATCC [GA]^GATC[CT]
XmnI 878 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AvaII 234 GGTCC G^G[AT]CC
1077 GGACC
BfaI 1039 CTAG C^TAG
1066 CTAG
BseRI 813 CTCCTC GAGGAG(10/8)
845 CTCCTC
BsmAI 783 GTCTC GTCTC(1/5)
843 GTCTC
BsmFI 112 GGGAC GGGAC(10/14)
657 GTCCC
Cfr13I 234 GGTCC G^G[ACGT]CC
1077 GGACC
MaeI 1039 CTAG C^TAG
1066 CTAG
MseI 707 TTAA T^TAA
719 TTAA
Sau96I 234 GGTCC G^G[ACGT]CC
1077 GGACC
SmlI 337 CTCAAG C^T[CT][GA]AG
540 CTTGAG
TfiI 98 GAATC G^A[AT]TC
533 GATTC
Tru9I 707 TTAA T^TAA
719 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BpmI BsaJI BsiYI BslI BsrI
BssKI BstNI Cac8I CfoI DdeI
DpnII EarI EcoRII Fnu4HI HaeIII
HhaI HinP1I HinfI HphI ItaI
Ksp632I MaeIII MboI MboII MnlI
MslI MvaI MwoI NdeII NlaIII
RsaI Sau3AI ScrFI TseI Tsp509I
TspRI