(Circular) (Six-base) MAPSORT of: pFN21AB9852.seq Check: 2898 from: 1 to: 5851 pFN21AB9852 5851 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3661 278 Size: 53 83 186 936 2125 2468 Fragments arranged by size: 2468 2125 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3194 1937 Size: 1257 4594 Acc65I G'GTAC_C Cuts at: 3172 3172 Size: 5851 AclI AA'CG_TT Cuts at: 1563 3978 4351 1563 Size: 2415 373 3063 Fragments arranged by size: 3063 2415 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3914 5298 5566 1632 Size: 2282 1384 268 1917 Fragments arranged by size: 2282 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5654 AflIII A'CryG_T Cuts at: 1203 4750 5727 1203 Size: 3547 977 1327 Fragments arranged by size: 3547 1327 977 AgeI A'CCGG_T Cuts at: 4682 4682 Size: 5851 AhdI GACnn_n'nnGTC Cuts at: 2785 4580 2785 Size: 1795 4056 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5851 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5851 AlwNI CAG_nnn'CTG Cuts at: 3594 5166 3594 Size: 1572 4279 ApaLI G'TGCA_C Cuts at: 3908 5064 3908 Size: 1156 4695 ApoI r'AATT_y Cuts at: 1493 3031 3160 3386 3538 5647 1493 Size: 1538 129 226 152 2109 1697 Fragments arranged by size: 2109 1697 1538 226 152 129 AseI AT'TA_AT Cuts at: 160 1032 4405 160 Size: 872 3373 1606 Fragments arranged by size: 3373 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5851 AvaI C'yCGr_G Cuts at: 1870 1943 3176 1870 Size: 73 1233 4545 Fragments arranged by size: 4545 1233 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2261 2294 2261 Size: 33 5818 BamHI G'GATC_C Cuts at: 3181 3548 3181 Size: 367 5484 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3172 4627 618 Size: 325 217 627 1385 1455 1842 Fragments arranged by size: 1842 1455 1385 627 325 217 BanII G_rGCy'C Cuts at: 1391 2771 3170 1391 Size: 1380 399 4072 Fragments arranged by size: 4072 1380 399 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4880 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4042 4076 1903 Size: 34 2105 34 3678 Fragments arranged by size: 3678 2105 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2489 3743 4953 2489 Size: 1254 1210 3387 Fragments arranged by size: 3387 1254 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5851 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4462 136 Size: 107 122 71 631 605 2790 1525 Fragments arranged by size: 2790 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5846 5846 Size: 5851 BlpI GC'TnA_GC Cuts at: 2765 3268 2765 Size: 503 5348 Bme1580I G_kGCm'C Cuts at: 1790 3912 5068 1790 Size: 2122 1156 2573 Fragments arranged by size: 2573 2122 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5851 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2941 3582 4540 Size: 416 53 83 399 24 1524 641 958 Cuts at: 4540 442 Size: 1753 Fragments arranged by size: 1753 1524 958 641 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5851 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2035 2085 3164 4511 1239 Size: 796 50 1079 1347 2579 Fragments arranged by size: 2579 1347 1079 796 50 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2327 2566 3976 4841 5139 2327 Size: 239 1410 865 298 3039 Fragments arranged by size: 3039 1410 865 298 239 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2793 4514 882 Size: 617 1294 1721 2219 Fragments arranged by size: 2219 1721 1294 617 BsaAI yAC'GTr Cuts at: 493 1204 3642 493 Size: 711 2438 2702 Fragments arranged by size: 2702 2438 711 BsaBI GATnn'nnATC Cuts at: 5845 5845 Size: 5851 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3658 4040 4675 275 Size: 53 83 186 936 2125 382 635 1451 Fragments arranged by size: 2125 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4284 4682 4956 5103 5514 4284 Size: 398 274 147 411 4621 Fragments arranged by size: 4621 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2058 2088 1178 Size: 30 165 30 655 30 4941 Fragments arranged by size: 4941 655 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2809 2841 1186 Size: 1623 32 4196 Fragments arranged by size: 4196 1623 32 BseYI C'CCAG_C Cuts at: 2113 5054 5550 2113 Size: 2941 496 2414 Fragments arranged by size: 2941 2414 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2967 1163 Size: 573 1231 4047 Fragments arranged by size: 4047 1231 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3301 4062 5090 1540 Size: 275 186 1300 761 1028 2301 Fragments arranged by size: 2301 1300 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3912 3997 5068 3912 Size: 85 1071 4695 Fragments arranged by size: 4695 1071 85 BsmI GAATG_Cn' Cuts at: 3362 3455 3362 Size: 93 5758 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2771 3170 3912 3997 5068 1391 Size: 399 981 399 742 85 1071 2174 Fragments arranged by size: 2174 1071 981 742 399 399 85 BspEI T'CCGG_A Cuts at: 5514 5514 Size: 5851 BspHI T'CATG_A Cuts at: 3740 5470 3740 Size: 1730 4121 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3206 844 Size: 2362 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2831 3738 1394 Size: 429 1008 907 3507 Fragments arranged by size: 3507 1008 907 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4346 4520 65 Size: 1183 3098 174 1396 Fragments arranged by size: 3098 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4495 4682 1700 Size: 110 2685 187 2869 Fragments arranged by size: 2869 2685 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5851 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5851 BssSI C'ACGA_G Cuts at: 3911 4923 3911 Size: 1012 4839 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5851 BstBI TT'CG_AA Cuts at: 4655 4655 Size: 5851 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5851 BstXI CCAn_nnnn'nTGG Cuts at: 2360 2360 Size: 5851 BstYI r'GATC_y Cuts at: 1972 2572 3181 3548 3933 3950 5402 5488 5500 Size: 600 609 367 385 17 1452 86 12 Cuts at: 5500 5846 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 609 600 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2023 2023 Size: 5851 BtgI C'CryG_G Cuts at: 513 1065 2004 2613 513 Size: 552 939 609 3751 Fragments arranged by size: 3751 939 609 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5112 Fragments arranged by size: 5112 683 56 BtsI GCAGTG_nn' Cuts at: 3367 4161 4181 3367 Size: 794 20 5037 Fragments arranged by size: 5037 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5851 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2764 2799 529 Size: 35 2200 35 3581 Fragments arranged by size: 3581 2200 35 35 DraI TTT'AAA Cuts at: 3155 3155 Size: 5851 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4858 809 Size: 507 3542 1802 Fragments arranged by size: 3542 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3298 4187 5816 Size: 54 1425 50 275 18 1468 889 1629 Cuts at: 5816 8 Size: 43 Fragments arranged by size: 1629 1468 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3298 1537 Size: 275 1486 4090 Fragments arranged by size: 4090 1486 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2307 2418 2489 2693 3781 1353 Size: 954 111 71 204 1088 3423 Fragments arranged by size: 3423 1088 954 204 111 71 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4438 4812 4958 387 Size: 1064 138 2849 374 146 1280 Fragments arranged by size: 2849 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2035 2085 3164 3914 4511 5298 5566 Size: 393 403 50 1079 750 597 787 268 Cuts at: 5566 1239 Size: 1524 Fragments arranged by size: 1524 1079 787 750 597 403 393 268 50 EcoNI CCTnn'n_nnAGG Cuts at: 2039 2039 Size: 5851 EcoO109I rG'GnC_Cy Cuts at: 2239 2239 Size: 5851 EcoRI G'AATT_C Cuts at: 3031 3160 3538 3031 Size: 129 378 5344 Fragments arranged by size: 5344 378 129 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2512 2512 Size: 5851 FspI TGC'GCA Cuts at: 4357 4357 Size: 5851 HaeII r_GCGC'y Cuts at: 1678 2022 4998 1678 Size: 344 2976 2531 Fragments arranged by size: 2976 2531 344 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2135 2167 2771 2803 4492 Size: 32 135 32 563 32 604 32 1689 Cuts at: 4492 4524 4566 4598 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1689 604 563 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3195 3441 669 Size: 988 281 1257 246 3079 Fragments arranged by size: 3079 1257 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5851 HpaI GTT'AAC Cuts at: 3441 3441 Size: 5851 KpnI G_GTAC'C Cuts at: 3176 3176 Size: 5851 MfeI C'AATT_G Cuts at: 3450 3450 Size: 5851 MluI A'CGCG_T Cuts at: 5727 5727 Size: 5851 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4965 5149 1305 Size: 3660 184 2007 Fragments arranged by size: 3660 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4029 Fragments arranged by size: 4029 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2255 2639 2645 3809 4168 4327 518 Size: 648 1089 384 6 1164 359 159 2042 Fragments arranged by size: 2042 1164 1089 648 384 359 159 6 MspA1I CmG'CkG Cuts at: 1606 2123 3267 3944 5092 5337 1606 Size: 517 1144 677 1148 245 2120 Fragments arranged by size: 2120 1148 1144 677 517 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4360 Fragments arranged by size: 4360 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5851 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5851 NotI GC'GGCC_GC Cuts at: 3298 3298 Size: 5851 NspI r_CATG'y Cuts at: 1132 1229 2380 3209 4754 1132 Size: 97 1151 829 1545 2229 Fragments arranged by size: 2229 1545 1151 829 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5851 PciI A'CATG_T Cuts at: 4750 4750 Size: 5851 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5851 PmeI GTTT'AAAC Cuts at: 3155 3155 Size: 5851 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3920 3952 5433 5465 3920 Size: 32 1481 32 4306 Fragments arranged by size: 4306 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2239 2239 Size: 5851 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5851 PsiI TTA'TAA Cuts at: 3421 3421 Size: 5851 PstI C_TGCA'G Cuts at: 830 2247 3203 830 Size: 1417 956 3478 Fragments arranged by size: 3478 1417 956 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5851 SalI G'TCGA_C Cuts at: 1936 3193 1936 Size: 1257 4594 SapI GCTCTTCn'nnn_ Cuts at: 2418 2418 Size: 5851 SbfI CC_TGCA'GG Cuts at: 3203 3203 Size: 5851 ScaI AGT'ACT Cuts at: 2088 4099 5811 2088 Size: 2011 1712 2128 Fragments arranged by size: 2128 2011 1712 SexAI A'CCwGG_T Cuts at: 2179 2179 Size: 5851 SfcI C'TryA_G Cuts at: 826 1045 2243 3199 4334 5015 5206 826 Size: 219 1198 956 1135 681 191 1471 Fragments arranged by size: 1471 1198 1135 956 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5851 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5851 SmaI CCC'GGG Cuts at: 1872 3178 1872 Size: 1306 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2342 2545 3955 4856 5118 820 Size: 197 926 399 203 1410 901 262 1553 Fragments arranged by size: 1553 1410 926 901 399 262 203 197 SnaBI TAC'GTA Cuts at: 493 3642 493 Size: 3149 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5851 SphI G_CATG'C Cuts at: 1132 3209 1132 Size: 2077 3774 SspI AAT'ATT Cuts at: 5 52 3775 5 Size: 47 3723 2081 Fragments arranged by size: 3723 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5851 StyI C'CwwG_G Cuts at: 513 1065 2004 2655 3290 513 Size: 552 939 651 635 3074 Fragments arranged by size: 3074 939 651 635 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 1997 3878 3895 4048 4233 4678 325 Size: 1255 417 1881 17 153 185 445 1498 Fragments arranged by size: 1881 1498 1255 445 417 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2086 4097 5809 Size: 274 80 33 51 1443 109 2011 1712 Cuts at: 5809 96 Size: 138 Fragments arranged by size: 2011 1712 1443 274 138 109 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2058 2090 5811 5843 2058 Size: 32 3721 32 2066 Fragments arranged by size: 3721 2066 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5851 XbaI T'CTAG_A Cuts at: 3187 3187 Size: 5851 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2753 2753 Size: 5851 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5851 XmaI C'CCGG_G Cuts at: 1870 3176 1870 Size: 1306 4545 XmnI GAAnn'nnTTC Cuts at: 2887 3980 2887 Size: 1093 4758 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3659 276 Size: 53 83 186 936 2125 2468 Fragments arranged by size: 2468 2125 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI