Restriction map for
pFN21AB9849
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AhdI AlwNI
AocI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI BamHI
BbeI BbrPI BcgI BciVI BclI
BfrI BglII BlpI BpmI Bpu1102I
BsaBI BsaI BseAI BseRI BsgI
BsmAI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssSI Bst1107I BstBI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI Eam1105I Eco47III Eco81I EcoNI
EcoRI EcoRV EcoT22I FbaI FseI
HincII HindII HindIII HpaI KasI
KpnI MamI MfeI MluI MroI
MseI MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SbfI ScaI SexAI
SfoI SfuI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI Tru9I Tsp509I
TthHB8I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AcyI 400 GGCGTC G[GA]^CG[CT]C
AgeI 389 ACCGGT A^CCGGT
Alw44I 385 GTGCAC G^TGCAC
AlwI 1019 GATCC GGATC(4/5)
ApaLI 385 GTGCAC G^TGCAC
AspI 1083 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 709 CCTAGG C^CTAGG
BalI 881 TGGCCA TGG^CCA
BbsI 757 GTCTTC GAAGAC(2/6)
BbvCI 825 CCTCAGC CCTCAGC(-5/-2)
BlnI 709 CCTAGG C^CTAGG
BpuAI 757 GTCTTC GAAGAC(2/6)
BsaHI 400 GGCGTC G[GA]^CG[CT]C
BsaWI 389 ACCGGT [AT]^CCGG[AT]
BsiEI 874 CGGCCG CG[GA][CT]^CG
BsiWI 939 CGTACG C^GTACG
BsmFI 691 GGGAC GGGAC(10/14)
BsmI 195 GAATGC GAATGC(1/-1)
BstAPI 238 GCACTGTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 867 GGTTACC G^GT[ACGT]ACC
BstPI 867 GGTTACC G^GT[ACGT]ACC
BstYI 1018 AGATCC [GA]^GATC[CT]
DpnII 1019 GATC ^GATC
EagI 874 CGGCCG C^GGCCG
EclXI 874 CGGCCG C^GGCCG
Eco52I 874 CGGCCG C^GGCCG
EcoO65I 867 GGTTACC G^GT[ACGT]ACC
EcoT14I 709 CCTAGG C^C[AT][AT]GG
HaeII 159 GGCGCT [GA]GCGC^[CT]
Hin1I 400 GGCGTC G[GA]^CG[CT]C
KspI 282 CCGCGG CCGC^GG
MboI 1019 GATC ^GATC
MflI 1018 AGATCC [GA]^GATC[CT]
MluNI 881 TGGCCA TGG^CCA
MscI 881 TGGCCA TGG^CCA
NaeI 1095 GCCGGC GCC^GGC
NdeII 1019 GATC ^GATC
NgoMIV 1095 GCCGGC G^CCGGC
NspI 1034 ACATGC [GA]CATG^[CT]
PflFI 1083 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 964 CCACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 389 ACCGGT A^CCGGT
PpuMI 691 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 828 CAGCTG CAG^CTG
SacI 568 GAGCTC GAGCT^C
SacII 282 CCGCGG CCGC^GG
Sau3AI 1019 GATC ^GATC
SfiI 486 GGCCTGCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 388 CACCGGTG C[GA]^CCGG[CT]G
SmlI 184 CTCAAG C^T[CT][GA]AG
StyI 709 CCTAGG C^C[AT][AT]GG
Tth111I 1083 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 964 CCACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1018 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 96 GTAC GT^AC
940 GTAC
ApaI 15 GGGCCC GGGCC^C
437 GGGCCC
AvaII 692 GGACC G^G[AT]CC
822 GGACC
AviII 731 TGCGCA TGC^GCA
1013 TGCGCA
BfaI 710 CTAG C^TAG
1076 CTAG
BglI 487 GCCTGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
985 GCCACCCCGGC
BsaAI 97 TACGTG [CT]AC^GT[GA]
652 TACGTG
BspMI 373 ACCTGC ACCTGC(4/8)
544 ACCTGC
BsrFI 389 ACCGGT [GA]^CCGG[CT]
1095 GCCGGC
BssHII 524 GCGCGC G^CGCGC
526 GCGCGC
BstXI 737 CCATCGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
848 CCATCAACATGG
Cfr10I 389 ACCGGT [GA]^CCGG[CT]
1095 GCCGGC
FspI 731 TGCGCA TGC^GCA
1013 TGCGCA
HgaI 199 GCGTC GACGC(5/10)
401 GCGTC
HinfI 1072 GACTC G^A[ACGT]TC
1087 GAGTC
MaeI 710 CTAG C^TAG
1076 CTAG
PleI 1072 GACTC GAGTC(4/5)
1087 GAGTC
PstI 354 CTGCAG CTGCA^G
999 CTGCAG
RsaI 96 GTAC GT^AC
940 GTAC
SapI 570 GCTCTTC GCTCTTC(1/4)
615 GCTCTTC
Tsp45I 514 GTCAC ^GT[GC]AC
787 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccII AciI AluI AspHI AvaI
BanI BanII BbvI BcnI BmyI
BsaJI BsiHKAI BsiYI BslI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DraII DsaI EaeI EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI Ksp632I MaeII MaeIII MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NlaIII NlaIV
Sau96I ScrFI SfaNI SfcI TseI
TspRI