(Circular) (Six-base) MAPSORT of: pFN21AB9848.seq Check: 9894 from: 1 to: 5833 pFN21AB9848 5833 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2835 2835 Size: 5833 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2810 3643 278 Size: 53 83 186 936 1274 833 2468 Fragments arranged by size: 2468 1274 936 833 186 83 53 AccI GT'mk_AC Cuts at: 1937 3176 1937 Size: 1239 4594 Acc65I G'GTAC_C Cuts at: 3154 3154 Size: 5833 AclI AA'CG_TT Cuts at: 1563 3132 3960 4333 1563 Size: 1569 828 373 3063 Fragments arranged by size: 3063 1569 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2432 2837 3896 5280 5548 1632 Size: 800 405 1059 1384 268 1917 Fragments arranged by size: 1917 1384 1059 800 405 268 AfeI AGC'GCT Cuts at: 2885 2885 Size: 5833 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5636 AflIII A'CryG_T Cuts at: 1203 2301 4732 5709 1203 Size: 1098 2431 977 1327 Fragments arranged by size: 2431 1327 1098 977 AgeI A'CCGG_T Cuts at: 4664 4664 Size: 5833 AhdI GACnn_n'nnGTC Cuts at: 2685 4562 2685 Size: 1877 3956 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5833 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5833 AlwNI CAG_nnn'CTG Cuts at: 3576 5148 3576 Size: 1572 4261 ApaI G_GGCC'C Cuts at: 2180 2276 2739 2180 Size: 96 463 5274 Fragments arranged by size: 5274 463 96 ApaLI G'TGCA_C Cuts at: 3890 5046 3890 Size: 1156 4677 ApoI r'AATT_y Cuts at: 1493 3142 3368 3520 5629 1493 Size: 1649 226 152 2109 1697 Fragments arranged by size: 2109 1697 1649 226 152 AseI AT'TA_AT Cuts at: 160 1032 4387 160 Size: 872 3355 1606 Fragments arranged by size: 3355 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5833 AvaI C'yCGr_G Cuts at: 1870 1943 2269 2437 2779 3046 3083 3158 1870 Size: 73 326 168 342 267 37 75 4545 Fragments arranged by size: 4545 342 326 267 168 75 73 37 AvrII C'CTAG_G Cuts at: 2551 2551 Size: 5833 BamHI G'GATC_C Cuts at: 3163 3530 3163 Size: 367 5466 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2381 2659 2933 3017 3154 Size: 325 217 627 594 278 274 84 137 Cuts at: 3154 4609 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 594 325 278 274 217 137 84 BanII G_rGCy'C Cuts at: 1391 2117 2180 2276 2728 2739 3081 3152 1391 Size: 726 63 96 452 11 342 71 4072 Fragments arranged by size: 4072 726 452 342 96 71 63 11 BbeI G_GCGC'C Cuts at: 3021 3021 Size: 5833 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2962 928 Size: 971 1063 3799 Fragments arranged by size: 3799 1063 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4024 4058 1903 Size: 34 50 34 2003 34 3678 Fragments arranged by size: 3678 2003 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3725 4935 3725 Size: 1210 4623 BclI T'GATC_A Cuts at: 1548 1548 Size: 5833 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4444 136 Size: 107 122 71 631 605 2772 1525 Fragments arranged by size: 2772 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5828 5828 Size: 5833 BlpI GC'TnA_GC Cuts at: 3250 3250 Size: 5833 Bme1580I G_kGCm'C Cuts at: 1790 2180 2276 2384 2494 2664 2739 3894 5050 Size: 390 96 108 110 170 75 1155 1156 Cuts at: 5050 1790 Size: 2573 Fragments arranged by size: 2573 1156 1155 390 170 110 108 96 75 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5833 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2729 3564 4522 Size: 416 53 83 399 24 1312 835 958 Cuts at: 4522 442 Size: 1753 Fragments arranged by size: 1753 1312 958 835 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5833 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2374 2374 Size: 5833 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2172 2726 2903 4493 1239 Size: 933 554 177 1590 2579 Fragments arranged by size: 2579 1590 933 554 177 Bpu10I CC'TnA_GC Cuts at: 2571 2671 2571 Size: 100 5733 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2586 3958 4823 5121 2586 Size: 1372 865 298 3298 Fragments arranged by size: 3298 1372 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2469 4496 882 Size: 617 970 2027 2219 Fragments arranged by size: 2219 2027 970 617 BsaAI yAC'GTr Cuts at: 493 1204 3624 493 Size: 711 2420 2702 Fragments arranged by size: 2702 2420 711 BsaBI GATnn'nnATC Cuts at: 5827 5827 Size: 5833 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2807 3018 3640 4022 Size: 53 83 186 936 1274 211 622 382 Cuts at: 4022 4657 275 Size: 635 1451 Fragments arranged by size: 1451 1274 936 635 622 382 211 186 83 53 BsaWI w'CCGG_w Cuts at: 4266 4664 4938 5085 5496 4266 Size: 398 274 147 411 4603 Fragments arranged by size: 4603 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2120 2150 1178 Size: 30 165 30 717 30 4861 Fragments arranged by size: 4861 717 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2212 3041 1186 Size: 1026 829 3978 Fragments arranged by size: 3978 1026 829 BseYI C'CCAG_C Cuts at: 2173 3035 5036 5532 2173 Size: 862 2001 496 2474 Fragments arranged by size: 2474 2001 862 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2973 1163 Size: 573 1237 4023 Fragments arranged by size: 4023 1237 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2657 2848 3283 4044 5072 1540 Size: 275 186 656 191 435 761 1028 2301 Fragments arranged by size: 2301 1028 761 656 435 275 191 186 BsiHKAI G_wGCw'C Cuts at: 3894 3979 5050 3894 Size: 85 1071 4677 Fragments arranged by size: 4677 1071 85 BsmI GAATG_Cn' Cuts at: 3344 3437 3344 Size: 93 5740 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2117 2180 2276 2384 2494 2664 2728 Size: 399 327 63 96 108 110 170 64 Cuts at: 2728 2739 3081 3152 3894 3979 5050 1391 Size: 11 342 71 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 342 327 170 110 108 96 85 71 64 63 11 BspEI T'CCGG_A Cuts at: 5496 5496 Size: 5833 BspHI T'CATG_A Cuts at: 3722 5452 3722 Size: 1730 4103 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2088 2243 2835 3188 844 Size: 1244 155 592 353 3489 Fragments arranged by size: 3489 1244 592 353 155 BsrBI CCG'CTC Cuts at: 1394 1823 3720 1394 Size: 429 1897 3507 Fragments arranged by size: 3507 1897 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4328 4502 65 Size: 1183 3080 174 1396 Fragments arranged by size: 3080 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2930 3062 4477 4664 1700 Size: 110 1120 132 1415 187 2869 Fragments arranged by size: 2869 1415 1120 187 132 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5833 BssHII G'CGCG_C Cuts at: 1925 2415 1925 Size: 490 5343 BssSI C'ACGA_G Cuts at: 2110 2359 3011 3893 4905 2110 Size: 249 652 882 1012 3038 Fragments arranged by size: 3038 1012 882 652 249 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5833 BstBI TT'CG_AA Cuts at: 4637 4637 Size: 5833 BstEII G'GTnAC_C Cuts at: 1184 2230 1184 Size: 1046 4787 BstXI CCAn_nnnn'nTGG Cuts at: 2392 2704 2392 Size: 312 5521 BstYI r'GATC_y Cuts at: 1972 3163 3530 3915 3932 5384 5470 5482 5828 Size: 1191 367 385 17 1452 86 12 346 Cuts at: 5828 1972 Size: 1977 Fragments arranged by size: 1977 1452 1191 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2118 2118 Size: 5833 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5281 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5094 Fragments arranged by size: 5094 683 56 BtsI GCAGTG_nn' Cuts at: 3078 3349 4143 4163 3078 Size: 271 794 20 4748 Fragments arranged by size: 4748 794 271 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5833 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2468 2503 529 Size: 35 1904 35 3859 Fragments arranged by size: 3859 1904 35 35 DraI TTT'AAA Cuts at: 3137 3137 Size: 5833 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4840 809 Size: 507 3524 1802 Fragments arranged by size: 3524 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2431 2991 3280 Size: 54 1425 50 275 18 601 560 289 Cuts at: 3280 4169 5798 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 601 560 289 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3280 1537 Size: 275 1468 4090 Fragments arranged by size: 4090 1468 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2876 3763 1353 Size: 1523 887 3423 Fragments arranged by size: 3423 1523 887 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2726 4420 4794 4940 387 Size: 1064 138 1137 1694 374 146 1280 Fragments arranged by size: 1694 1280 1137 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2172 2432 2726 2837 2903 3896 4493 Size: 393 540 260 294 111 66 993 597 Cuts at: 4493 5280 5548 1239 Size: 787 268 1524 Fragments arranged by size: 1524 993 787 597 540 393 294 268 260 111 66 EcoO109I rG'GnC_Cy Cuts at: 2217 2242 2273 2368 2217 Size: 25 31 95 5682 Fragments arranged by size: 5682 95 31 25 EcoRI G'AATT_C Cuts at: 3142 3520 3142 Size: 378 5455 FspI TGC'GCA Cuts at: 2514 2978 4339 2514 Size: 464 1361 4008 Fragments arranged by size: 4008 1361 464 FspAI rTGC'GCAy Cuts at: 2514 2514 Size: 5833 HaeII r_GCGC'y Cuts at: 1678 2054 2447 2887 2914 3021 4980 1678 Size: 376 393 440 27 107 1959 2531 Fragments arranged by size: 2531 1959 440 393 376 107 27 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2120 2152 2671 2703 4474 Size: 32 135 32 548 32 519 32 1771 Cuts at: 4474 4506 4548 4580 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1771 548 519 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3177 3423 669 Size: 988 281 1239 246 3079 Fragments arranged by size: 3079 1239 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5833 HpaI GTT'AAC Cuts at: 3423 3423 Size: 5833 KasI G'GCGC_C Cuts at: 3017 3017 Size: 5833 KpnI G_GTAC'C Cuts at: 3158 3158 Size: 5833 MfeI C'AATT_G Cuts at: 3432 3432 Size: 5833 MluI A'CGCG_T Cuts at: 5709 5709 Size: 5833 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2452 3153 4947 5131 1305 Size: 1147 701 1794 184 2007 Fragments arranged by size: 2007 1794 1147 701 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2433 2993 10 Size: 54 1425 343 601 560 2850 Fragments arranged by size: 2850 1425 601 560 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2313 2508 3791 4150 4309 518 Size: 648 1147 195 1283 359 159 2042 Fragments arranged by size: 2042 1283 1147 648 359 195 159 MspA1I CmG'CkG Cuts at: 1606 2015 2143 2173 2388 2531 2841 3249 3926 Size: 409 128 30 215 143 310 408 677 Cuts at: 3926 5074 5319 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 409 408 310 245 215 143 128 30 NaeI GCC'GGC Cuts at: 3064 3064 Size: 5833 NarI GG'CG_CC Cuts at: 3018 3018 Size: 5833 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5281 NdeI CA'TA_TG Cuts at: 387 387 Size: 5833 NgoMIV G'CCGG_C Cuts at: 3062 3062 Size: 5833 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5833 NotI GC'GGCC_GC Cuts at: 3280 3280 Size: 5833 NspI r_CATG'y Cuts at: 1132 1229 2305 2513 3191 4736 1132 Size: 97 1076 208 678 1545 2229 Fragments arranged by size: 2229 1545 1076 678 208 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5833 PciI A'CATG_T Cuts at: 2301 4732 2301 Size: 2431 3402 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5833 PmeI GTTT'AAAC Cuts at: 3137 3137 Size: 5833 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3902 3934 5415 5447 3902 Size: 32 1481 32 4288 Fragments arranged by size: 4288 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2242 2368 2242 Size: 126 5707 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5833 PsiI TTA'TAA Cuts at: 3403 3403 Size: 5833 PspOMI G'GGCC_C Cuts at: 2176 2272 2735 2176 Size: 96 463 5274 Fragments arranged by size: 5274 463 96 PstI C_TGCA'G Cuts at: 830 2085 2148 2163 2295 2910 2963 3185 830 Size: 1255 63 15 132 615 53 222 3478 Fragments arranged by size: 3478 1255 615 222 132 63 53 15 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5833 PvuII CAG'CTG Cuts at: 2388 2531 2388 Size: 143 5690 SalI G'TCGA_C Cuts at: 1936 3175 1936 Size: 1239 4594 SapI GCTCTTCn'nnn_ Cuts at: 2876 2876 Size: 5833 SbfI CC_TGCA'GG Cuts at: 3185 3185 Size: 5833 ScaI AGT'ACT Cuts at: 4081 5793 4081 Size: 1712 4121 SfcI C'TryA_G Cuts at: 826 1045 2081 2144 2159 2291 2906 2959 3181 Size: 219 1036 63 15 132 615 53 222 Cuts at: 3181 4316 4997 5188 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1036 681 615 222 219 191 132 63 53 15 SfoI GGC'GCC Cuts at: 3019 3019 Size: 5833 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5833 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5833 SmaI CCC'GGG Cuts at: 1872 2271 2439 2781 3085 3160 1872 Size: 399 168 342 304 75 4545 Fragments arranged by size: 4545 399 342 304 168 75 SmlI C'TyrA_G Cuts at: 820 1017 1943 2601 3937 4838 5100 820 Size: 197 926 658 1336 901 262 1553 Fragments arranged by size: 1553 1336 926 901 658 262 197 SnaBI TAC'GTA Cuts at: 493 3624 493 Size: 3131 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5833 SphI G_CATG'C Cuts at: 1132 2513 3191 1132 Size: 1381 678 3774 Fragments arranged by size: 3774 1381 678 SspI AAT'ATT Cuts at: 5 52 3757 5 Size: 47 3705 2081 Fragments arranged by size: 3705 2081 47 StuI AGG'CCT Cuts at: 1517 2576 1517 Size: 1059 4774 StyI C'CwwG_G Cuts at: 513 1065 2262 2551 2577 3021 3272 513 Size: 552 1197 289 26 444 251 3074 Fragments arranged by size: 3074 1197 552 444 289 251 26 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2355 3860 3877 4030 4215 4660 325 Size: 1255 775 1505 17 153 185 445 1498 Fragments arranged by size: 1505 1498 1255 775 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4079 5791 96 Size: 274 80 33 51 1443 2102 1712 138 Fragments arranged by size: 2102 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2345 2377 5793 5825 2345 Size: 32 3416 32 2353 Fragments arranged by size: 3416 2353 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5833 XbaI T'CTAG_A Cuts at: 3169 3169 Size: 5833 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5833 XmaI C'CCGG_G Cuts at: 1870 2269 2437 2779 3083 3158 1870 Size: 399 168 342 304 75 4545 Fragments arranged by size: 4545 399 342 304 168 75 XmnI GAAnn'nnTTC Cuts at: 3962 3962 Size: 5833 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2808 3641 276 Size: 53 83 186 936 1274 833 2468 Fragments arranged by size: 2468 1274 936 833 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI XcmI