(Circular) (Six-base) MAPSORT of: pFN21AB9846.seq Check: 7598 from: 1 to: 5827 pFN21AB9846 5827 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2277 3637 278 Size: 53 83 186 936 741 1360 2468 Fragments arranged by size: 2468 1360 936 741 186 83 53 AccI GT'mk_AC Cuts at: 1937 3170 1937 Size: 1233 4594 Acc65I G'GTAC_C Cuts at: 3148 3148 Size: 5827 AclI AA'CG_TT Cuts at: 1563 2854 3954 4327 1563 Size: 1291 1100 373 3063 Fragments arranged by size: 3063 1291 1100 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2630 2855 3890 5274 5542 1632 Size: 998 225 1035 1384 268 1917 Fragments arranged by size: 1917 1384 1035 998 268 225 AfeI AGC'GCT Cuts at: 2588 2811 2588 Size: 223 5604 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5630 AflIII A'CryG_T Cuts at: 1203 4726 5703 1203 Size: 3523 977 1327 Fragments arranged by size: 3523 1327 977 AgeI A'CCGG_T Cuts at: 4658 4658 Size: 5827 AhdI GACnn_n'nnGTC Cuts at: 4556 4556 Size: 5827 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5827 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5827 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2664 2696 2664 Size: 32 5795 AlwNI CAG_nnn'CTG Cuts at: 2026 2079 3570 5142 2026 Size: 53 1491 1572 2711 Fragments arranged by size: 2711 1572 1491 53 ApaI G_GGCC'C Cuts at: 2311 2311 Size: 5827 ApaLI G'TGCA_C Cuts at: 2432 3884 5040 2432 Size: 1452 1156 3219 Fragments arranged by size: 3219 1452 1156 ApoI r'AATT_y Cuts at: 1493 2734 3136 3362 3514 5623 1493 Size: 1241 402 226 152 2109 1697 Fragments arranged by size: 2109 1697 1241 402 226 152 AseI AT'TA_AT Cuts at: 160 1032 4381 160 Size: 872 3349 1606 Fragments arranged by size: 3349 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5827 AvaI C'yCGr_G Cuts at: 1870 1943 2312 2359 3152 1870 Size: 73 369 47 793 4545 Fragments arranged by size: 4545 793 369 73 47 BamHI G'GATC_C Cuts at: 3157 3524 3157 Size: 367 5460 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3148 4603 618 Size: 325 217 627 1361 1455 1842 Fragments arranged by size: 1842 1455 1361 627 325 217 BanII G_rGCy'C Cuts at: 1391 2311 3146 1391 Size: 920 835 4072 Fragments arranged by size: 4072 920 835 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2501 928 Size: 971 602 4254 Fragments arranged by size: 4254 971 602 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2012 2046 4018 4052 1903 Size: 34 75 34 1972 34 3678 Fragments arranged by size: 3678 1972 75 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3719 4929 3719 Size: 1210 4617 BclI T'GATC_A Cuts at: 1548 1548 Size: 5827 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4438 136 Size: 107 122 71 631 605 2766 1525 Fragments arranged by size: 2766 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5822 5822 Size: 5827 BlpI GC'TnA_GC Cuts at: 3244 3244 Size: 5827 Bme1580I G_kGCm'C Cuts at: 1790 2311 2436 2913 3888 5044 1790 Size: 521 125 477 975 1156 2573 Fragments arranged by size: 2573 1156 975 521 477 125 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5827 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2854 3558 4516 Size: 416 53 83 399 24 1437 704 958 Cuts at: 4516 442 Size: 1753 Fragments arranged by size: 1753 1437 958 704 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5827 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2198 2318 2459 2537 2715 3011 4487 1239 Size: 959 120 141 78 178 296 1476 2579 Fragments arranged by size: 2579 1476 959 296 178 141 120 78 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2041 3952 4817 5115 2041 Size: 1911 865 298 2753 Fragments arranged by size: 2753 1911 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2059 2987 3107 4490 882 Size: 617 560 928 120 1383 2219 Fragments arranged by size: 2219 1383 928 617 560 120 BsaAI yAC'GTr Cuts at: 493 1204 3618 493 Size: 711 2414 2702 Fragments arranged by size: 2702 2414 711 BsaBI GATnn'nnATC Cuts at: 5821 5821 Size: 5827 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2274 2364 2373 3634 Size: 53 83 186 936 741 90 9 1261 Cuts at: 3634 4016 4651 275 Size: 382 635 1451 Fragments arranged by size: 1451 1261 936 741 635 382 186 90 83 53 9 BsaWI w'CCGG_w Cuts at: 4260 4658 4932 5079 5490 4260 Size: 398 274 147 411 4597 Fragments arranged by size: 4597 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5602 Fragments arranged by size: 5602 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2396 2933 1186 Size: 1210 537 4080 Fragments arranged by size: 4080 1210 537 BseYI C'CCAG_C Cuts at: 2770 2806 5030 5526 2770 Size: 36 2224 496 3071 Fragments arranged by size: 3071 2224 496 36 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2174 2360 1163 Size: 573 438 186 4630 Fragments arranged by size: 4630 573 438 186 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3277 4038 5066 1540 Size: 275 186 1276 761 1028 2301 Fragments arranged by size: 2301 1276 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2436 3888 3973 5044 2436 Size: 1452 85 1071 3219 Fragments arranged by size: 3219 1452 1071 85 BsmI GAATG_Cn' Cuts at: 3338 3431 3338 Size: 93 5734 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2311 2436 2913 3146 3888 3973 5044 Size: 399 521 125 477 233 742 85 1071 Cuts at: 5044 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 521 477 399 233 125 85 BspEI T'CCGG_A Cuts at: 5490 5490 Size: 5827 BspHI T'CATG_A Cuts at: 3716 5446 3716 Size: 1730 4097 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2133 3182 844 Size: 1289 1049 3489 Fragments arranged by size: 3489 1289 1049 BsrBI CCG'CTC Cuts at: 1394 1823 2182 3714 1394 Size: 429 359 1532 3507 Fragments arranged by size: 3507 1532 429 359 BsrDI GCAATG_nn' Cuts at: 65 1248 4322 4496 65 Size: 1183 3074 174 1396 Fragments arranged by size: 3074 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2418 4471 4658 1700 Size: 110 608 2053 187 2869 Fragments arranged by size: 2869 2053 608 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5827 BssHII G'CGCG_C Cuts at: 1925 2117 1925 Size: 192 5635 BssSI C'ACGA_G Cuts at: 3887 4899 3887 Size: 1012 4815 BstAPI GCAn_nnn'nTGC Cuts at: 794 2440 794 Size: 1646 4181 BstBI TT'CG_AA Cuts at: 4631 4631 Size: 5827 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5827 BstYI r'GATC_y Cuts at: 1972 3157 3524 3909 3926 5378 5464 5476 5822 Size: 1185 367 385 17 1452 86 12 346 Cuts at: 5822 1972 Size: 1977 Fragments arranged by size: 1977 1452 1185 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4336 Fragments arranged by size: 4336 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5088 Fragments arranged by size: 5088 683 56 BtsI GCAGTG_nn' Cuts at: 2117 3343 4137 4157 2117 Size: 1226 794 20 3787 Fragments arranged by size: 3787 1226 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5827 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5792 DraI TTT'AAA Cuts at: 3131 3131 Size: 5827 DraIII CAC_nnn'GTG Cuts at: 2338 2338 Size: 5827 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4834 809 Size: 507 3518 1802 Fragments arranged by size: 3518 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3274 4163 5792 Size: 54 1425 50 275 18 1444 889 1629 Cuts at: 5792 8 Size: 43 Fragments arranged by size: 1629 1444 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3274 1537 Size: 275 1462 4090 Fragments arranged by size: 4090 1462 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2658 3069 3757 1353 Size: 1305 411 688 3423 Fragments arranged by size: 3423 1305 688 411 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2519 4414 4788 4934 387 Size: 1064 138 930 1895 374 146 1280 Fragments arranged by size: 1895 1280 1064 930 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2198 2318 2459 2537 2630 2715 2855 Size: 393 566 120 141 78 93 85 140 Cuts at: 2855 3011 3890 4487 5274 5542 1239 Size: 156 879 597 787 268 1524 Fragments arranged by size: 1524 879 787 597 566 393 268 156 141 140 120 93 85 78 EcoNI CCTnn'n_nnAGG Cuts at: 2948 2948 Size: 5827 EcoO109I rG'GnC_Cy Cuts at: 2083 2257 2308 2953 2083 Size: 174 51 645 4957 Fragments arranged by size: 4957 645 174 51 EcoRI G'AATT_C Cuts at: 2734 3136 3514 2734 Size: 402 378 5047 Fragments arranged by size: 5047 402 378 FspI TGC'GCA Cuts at: 4333 4333 Size: 5827 HaeII r_GCGC'y Cuts at: 1678 2146 2590 2630 2813 4974 1678 Size: 468 444 40 183 2161 2531 Fragments arranged by size: 2531 2161 468 444 183 40 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4468 4500 4542 4574 1373 Size: 32 135 32 2896 32 42 32 2626 Fragments arranged by size: 2896 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2852 3171 3417 669 Size: 988 281 914 319 246 3079 Fragments arranged by size: 3079 988 914 319 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5827 HpaI GTT'AAC Cuts at: 3417 3417 Size: 5827 KpnI G_GTAC'C Cuts at: 3152 3152 Size: 5827 MfeI C'AATT_G Cuts at: 3426 3426 Size: 5827 MluI A'CGCG_T Cuts at: 5703 5703 Size: 5827 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2649 2869 4941 5125 1305 Size: 1344 220 2072 184 2007 Fragments arranged by size: 2072 2007 1344 220 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4005 Fragments arranged by size: 4005 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3785 4144 4303 518 Size: 648 2619 359 159 2042 Fragments arranged by size: 2619 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3243 3920 5068 5313 1606 Size: 1637 677 1148 245 2120 Fragments arranged by size: 2120 1637 1148 677 245 NaeI GCC'GGC Cuts at: 2420 2420 Size: 5827 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4336 Fragments arranged by size: 4336 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5827 NgoMIV G'CCGG_C Cuts at: 2418 2418 Size: 5827 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5827 NotI GC'GGCC_GC Cuts at: 3274 3274 Size: 5827 NspI r_CATG'y Cuts at: 1132 1229 2440 3185 4730 1132 Size: 97 1211 745 1545 2229 Fragments arranged by size: 2229 1545 1211 745 97 PasI CC'CwG_GG Cuts at: 1348 2913 1348 Size: 1565 4262 PciI A'CATG_T Cuts at: 4726 4726 Size: 5827 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5827 PfoI T'CCnGG_A Cuts at: 2236 2367 2475 2236 Size: 131 108 5588 Fragments arranged by size: 5588 131 108 PmeI GTTT'AAAC Cuts at: 3131 3131 Size: 5827 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3896 3928 5409 5441 3896 Size: 32 1481 32 4282 Fragments arranged by size: 4282 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2083 2083 Size: 5827 PshAI GACnn'nnGTC Cuts at: 1613 2847 1613 Size: 1234 4593 PsiI TTA'TAA Cuts at: 3397 3397 Size: 5827 PspOMI G'GGCC_C Cuts at: 2307 2307 Size: 5827 PstI C_TGCA'G Cuts at: 830 2022 2529 3179 830 Size: 1192 507 650 3478 Fragments arranged by size: 3478 1192 650 507 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5827 SalI G'TCGA_C Cuts at: 1936 3169 1936 Size: 1233 4594 SanDI GG'GwC_CC Cuts at: 2083 2083 Size: 5827 SapI GCTCTTCn'nnn_ Cuts at: 3069 3069 Size: 5827 SbfI CC_TGCA'GG Cuts at: 3179 3179 Size: 5827 ScaI AGT'ACT Cuts at: 4075 5787 4075 Size: 1712 4115 SexAI A'CCwGG_T Cuts at: 2334 3028 2334 Size: 694 5133 SfcI C'TryA_G Cuts at: 826 1045 2018 2455 2525 3123 3175 4310 4991 Size: 219 973 437 70 598 52 1135 681 Cuts at: 4991 5182 826 Size: 191 1471 Fragments arranged by size: 1471 1135 973 681 598 437 219 191 70 52 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5827 SgrAI Cr'CCGG_yG Cuts at: 1700 2418 1700 Size: 718 5109 SmaI CCC'GGG Cuts at: 1872 2361 3154 1872 Size: 489 793 4545 Fragments arranged by size: 4545 793 489 SmlI C'TyrA_G Cuts at: 820 1017 1943 2056 3931 4832 5094 820 Size: 197 926 113 1875 901 262 1553 Fragments arranged by size: 1875 1553 926 901 262 197 113 SnaBI TAC'GTA Cuts at: 493 3618 493 Size: 3125 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5827 SphI G_CATG'C Cuts at: 1132 3185 1132 Size: 2053 3774 SrfI GCCC'GGGC Cuts at: 2361 2361 Size: 5827 SspI AAT'ATT Cuts at: 5 52 3751 5 Size: 47 3699 2081 Fragments arranged by size: 3699 2081 47 StuI AGG'CCT Cuts at: 1517 3114 1517 Size: 1597 4230 StyI C'CwwG_G Cuts at: 513 1065 2004 3266 513 Size: 552 939 1262 3074 Fragments arranged by size: 3074 1262 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2252 2820 3854 3871 4024 4209 4654 Size: 1255 672 568 1034 17 153 185 445 Cuts at: 4654 325 Size: 1498 Fragments arranged by size: 1498 1255 1034 672 568 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4073 5785 96 Size: 274 80 33 51 1443 2096 1712 138 Fragments arranged by size: 2096 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5787 5819 5787 Size: 32 5795 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5827 XbaI T'CTAG_A Cuts at: 3163 3163 Size: 5827 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2144 2690 2144 Size: 546 5281 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5827 XmaI C'CCGG_G Cuts at: 1870 2359 3152 1870 Size: 489 793 4545 Fragments arranged by size: 4545 793 489 XmnI GAAnn'nnTTC Cuts at: 2013 2603 3956 2013 Size: 590 1353 3884 Fragments arranged by size: 3884 1353 590 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2275 3635 276 Size: 53 83 186 936 741 1360 2468 Fragments arranged by size: 2468 1360 936 741 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SfiI SfoI SwaI