(Linear) (Six-base) MAPSORT of: KIBB9843.seq  Check: 5465  from: 1  to: 1098



KIBB9843.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:12  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     219     995    1098 
   Size:        219     776     103

  Fragments arranged by size: 

                 776     219     103
 
AlwNI CAG_nnn'CTG

Cuts at:      0     382    1098 
   Size:        382     716
 
ApoI r'AATT_y

Cuts at:      0      12      68     779     811     968    1098 
   Size:         12      56     711      32     157     130

  Fragments arranged by size: 

                 711     157     130      56      32      12
 
AseI AT'TA_AT

Cuts at:      0     889    1098 
   Size:        889     209
 
BanI G'GyrC_C

Cuts at:      0     485    1098 
   Size:        485     613
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1061    1098 
   Size:       1061      37
 
BclI T'GATC_A

Cuts at:      0      21    1098 
   Size:         21    1077
 
BfrBI ATG'CAT

Cuts at:      0     515    1098 
   Size:        515     583
 
BglII A'GATC_T

Cuts at:      0     845    1098 
   Size:        845     253
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     501    1098 
   Size:        501     597
 
BsaAI yAC'GTr

Cuts at:      0     323    1098 
   Size:        323     775
 
BsaBI GATnn'nnATC

Cuts at:      0     362     592    1098 
   Size:        362     230     506

  Fragments arranged by size: 

                 506     362     230
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     461     682    1098 
   Size:        461     221     416

  Fragments arranged by size: 

                 461     416     221
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     303    1098 
   Size:        145     158     795

  Fragments arranged by size: 

                 795     158     145
 
BsiHKAI G_wGCw'C

Cuts at:      0     328    1098 
   Size:        328     770
 
BsmI GAATG_Cn'

Cuts at:      0      62     517    1069    1098 
   Size:         62     455     552      29

  Fragments arranged by size: 

                 552     455      62      29
 
Bsp1286I G_dGCh'C

Cuts at:      0     328    1098 
   Size:        328     770
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     322    1098 
   Size:        322     776
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      72     882     993    1098 
   Size:         72     810     111     105

  Fragments arranged by size: 

                 810     111     105      72
 
BstYI r'GATC_y

Cuts at:      0     845    1098 
   Size:        845     253
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     566     601    1098 
   Size:        566      35     497

  Fragments arranged by size: 

                 566     497      35
 
EaeI y'GGCC_r

Cuts at:      0     678    1098 
   Size:        678     420
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     219     501     995    1098 
   Size:        219     282     494     103

  Fragments arranged by size: 

                 494     282     219     103
 
EcoRI G'AATT_C

Cuts at:      0     811    1098 
   Size:        811     287
 
HincII GTy'rAC

Cuts at:      0     306    1098 
   Size:        306     792
 
HindIII A'AGCT_T

Cuts at:      0    1078    1098 
   Size:       1078      20
 
MscI TGG'CCA

Cuts at:      0     680    1098 
   Size:        680     418
 
NsiI A_TGCA'T

Cuts at:      0     517    1098 
   Size:        517     581
 
NspI r_CATG'y

Cuts at:      0     418    1098 
   Size:        418     680
 
PasI CC'CwG_GG

Cuts at:      0     882    1098 
   Size:        882     216
 
PmlI CAC'GTG

Cuts at:      0     323    1098 
   Size:        323     775
 
PsiI TTA'TAA

Cuts at:      0    1024    1098 
   Size:       1024      74
 
SfcI C'TryA_G

Cuts at:      0     347    1098 
   Size:        347     751
 
SphI G_CATG'C

Cuts at:      0     418    1098 
   Size:        418     680
 
StyI C'CwwG_G

Cuts at:      0     489    1098 
   Size:        489     609
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     886    1098 
   Size:        886     212
 
TatI w'GTAC_w

Cuts at:      0     735    1098 
   Size:        735     363
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     523     555    1098 
   Size:        523      32     543

  Fragments arranged by size: 

                 543     523      32
 
XmnI GAAnn'nnTTC

Cuts at:      0    1069    1098 
   Size:       1069      29
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI     AseI     BanI     BbsI     BclI    BfrBI
    BglII     BpmI    BsaAI    BsaBI    BseRI     BsgI  BsiHKAI     BsmI
 Bsp1286I    BspMI    BstXI    BstYI    CspCI     EaeI  Eco57MI    EcoRI
   HincII  HindIII     MscI     NsiI     NspI     PasI     PmlI     PsiI
     SfcI     SphI     StyI    TaqII     TatI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI
     BcgI    BciVI     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI    BsaXI    BseYI
    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI     DraI   DraIII     DrdI     EagI
     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     PacI     PciI    PflMI     PfoI
     PmeI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI