(Circular) (Six-base) MAPSORT of: pFN21AB9839.seq Check: 6365 from: 1 to: 5770 pFN21AB9839 5770 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3580 278 Size: 53 83 186 936 2044 2468 Fragments arranged by size: 2468 2044 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3113 1937 Size: 1176 4594 Acc65I G'GTAC_C Cuts at: 3091 3091 Size: 5770 AclI AA'CG_TT Cuts at: 1563 3897 4270 1563 Size: 2334 373 3063 Fragments arranged by size: 3063 2334 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3833 5217 5485 1632 Size: 2201 1384 268 1917 Fragments arranged by size: 2201 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5573 AflIII A'CryG_T Cuts at: 1203 2364 4669 5646 1203 Size: 1161 2305 977 1327 Fragments arranged by size: 2305 1327 1161 977 AgeI A'CCGG_T Cuts at: 4601 4601 Size: 5770 AhdI GACnn_n'nnGTC Cuts at: 4499 4499 Size: 5770 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5770 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5770 AlwNI CAG_nnn'CTG Cuts at: 2963 3513 5085 2963 Size: 550 1572 3648 Fragments arranged by size: 3648 1572 550 ApaLI G'TGCA_C Cuts at: 3827 4983 3827 Size: 1156 4614 ApoI r'AATT_y Cuts at: 1493 2036 2160 2302 2633 3079 3305 3457 5566 Size: 543 124 142 331 446 226 152 2109 Cuts at: 5566 1493 Size: 1697 Fragments arranged by size: 2109 1697 543 446 331 226 152 142 124 AseI AT'TA_AT Cuts at: 160 1032 4324 160 Size: 872 3292 1606 Fragments arranged by size: 3292 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5770 AvaI C'yCGr_G Cuts at: 1870 1943 3095 1870 Size: 73 1152 4545 Fragments arranged by size: 4545 1152 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2960 2993 2960 Size: 33 5737 BamHI G'GATC_C Cuts at: 3100 3467 3100 Size: 367 5403 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2610 3091 4546 618 Size: 325 217 627 823 481 1455 1842 Fragments arranged by size: 1842 1455 823 627 481 325 217 BanII G_rGCy'C Cuts at: 1391 2814 3089 1391 Size: 1423 275 4072 Fragments arranged by size: 4072 1423 275 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2216 928 Size: 971 317 4482 Fragments arranged by size: 4482 971 317 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3961 3995 1903 Size: 34 2024 34 3678 Fragments arranged by size: 3678 2024 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3662 4872 3662 Size: 1210 4560 BclI T'GATC_A Cuts at: 1548 2394 2604 1548 Size: 846 210 4714 Fragments arranged by size: 4714 846 210 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4381 136 Size: 107 122 71 631 605 2709 1525 Fragments arranged by size: 2709 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5765 5765 Size: 5770 BlpI GC'TnA_GC Cuts at: 3187 3187 Size: 5770 Bme1580I G_kGCm'C Cuts at: 1790 3831 4987 1790 Size: 2041 1156 2573 Fragments arranged by size: 2573 2041 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5770 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2128 2350 3501 Size: 416 53 83 399 24 711 222 1151 Cuts at: 3501 4459 442 Size: 958 1753 Fragments arranged by size: 1753 1151 958 711 416 399 222 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5770 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2096 2710 4430 1239 Size: 857 614 1720 2579 Fragments arranged by size: 2579 1720 857 614 Bpu10I CC'TnA_GC Cuts at: 3023 3023 Size: 5770 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2441 2957 3051 3895 4760 5058 2441 Size: 516 94 844 865 298 3153 Fragments arranged by size: 3153 865 844 516 298 94 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2128 4433 882 Size: 617 629 2305 2219 Fragments arranged by size: 2305 2219 629 617 BsaAI yAC'GTr Cuts at: 493 1204 3561 493 Size: 711 2357 2702 Fragments arranged by size: 2702 2357 711 BsaBI GATnn'nnATC Cuts at: 5764 5764 Size: 5770 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3577 3959 4594 275 Size: 53 83 186 936 2044 382 635 1451 Fragments arranged by size: 2044 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2949 4203 4601 4875 5022 5433 2949 Size: 1254 398 274 147 411 3286 Fragments arranged by size: 3286 1254 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2108 2138 1178 Size: 30 165 30 705 30 4810 Fragments arranged by size: 4810 705 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2105 1186 Size: 919 4851 BseYI C'CCAG_C Cuts at: 2104 4973 5469 2104 Size: 2869 496 2405 Fragments arranged by size: 2869 2405 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5197 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3220 3981 5009 1540 Size: 275 186 1219 761 1028 2301 Fragments arranged by size: 2301 1219 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2814 3831 3916 4987 2814 Size: 1017 85 1071 3597 Fragments arranged by size: 3597 1071 1017 85 BsmI GAATG_Cn' Cuts at: 2882 3281 3374 2882 Size: 399 93 5278 Fragments arranged by size: 5278 399 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2814 3089 3831 3916 4987 1391 Size: 399 1024 275 742 85 1071 2174 Fragments arranged by size: 2174 1071 1024 742 399 275 85 BspEI T'CCGG_A Cuts at: 5433 5433 Size: 5770 BspHI T'CATG_A Cuts at: 3659 5389 3659 Size: 1730 4040 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3125 844 Size: 2281 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3657 1394 Size: 429 1834 3507 Fragments arranged by size: 3507 1834 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2244 4265 4439 65 Size: 1183 996 2021 174 1396 Fragments arranged by size: 2021 1396 1183 996 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4414 4601 1700 Size: 110 2604 187 2869 Fragments arranged by size: 2869 2604 187 110 BsrGI T'GTAC_A Cuts at: 96 2280 96 Size: 2184 3586 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5770 BssSI C'ACGA_G Cuts at: 3830 4842 3830 Size: 1012 4758 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5770 BstBI TT'CG_AA Cuts at: 4574 4574 Size: 5770 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5770 BstXI CCAn_nnnn'nTGG Cuts at: 2878 2878 Size: 5770 BstYI r'GATC_y Cuts at: 1972 3100 3467 3852 3869 5321 5407 5419 5765 Size: 1128 367 385 17 1452 86 12 346 Cuts at: 5765 1972 Size: 1977 Fragments arranged by size: 1977 1452 1128 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4279 Fragments arranged by size: 4279 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5031 Fragments arranged by size: 5031 683 56 BtsI GCAGTG_nn' Cuts at: 2349 3286 4080 4100 2349 Size: 937 794 20 4019 Fragments arranged by size: 4019 937 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5770 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5735 DraI TTT'AAA Cuts at: 3074 3074 Size: 5770 DraIII CAC_nnn'GTG Cuts at: 2226 2845 2985 2226 Size: 619 140 5011 Fragments arranged by size: 5011 619 140 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4777 809 Size: 507 3461 1802 Fragments arranged by size: 3461 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2295 3217 4106 Size: 54 1425 50 275 18 465 922 889 Cuts at: 4106 5735 8 Size: 1629 43 Fragments arranged by size: 1629 1425 922 889 465 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3217 1537 Size: 275 1405 4090 Fragments arranged by size: 4090 1405 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2657 3700 1353 Size: 1304 1043 3423 Fragments arranged by size: 3423 1304 1043 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4357 4731 4877 387 Size: 1064 138 2768 374 146 1280 Fragments arranged by size: 2768 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2812 2812 Size: 5770 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2096 2710 3833 4430 5217 5485 1239 Size: 393 464 614 1123 597 787 268 1524 Fragments arranged by size: 1524 1123 787 614 597 464 393 268 EcoRI G'AATT_C Cuts at: 2160 3079 3457 2160 Size: 919 378 4473 Fragments arranged by size: 4473 919 378 FspI TGC'GCA Cuts at: 4276 4276 Size: 5770 HaeII r_GCGC'y Cuts at: 1678 4917 1678 Size: 3239 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2054 2086 2773 2805 4411 Size: 32 135 32 482 32 687 32 1606 Cuts at: 4411 4443 4485 4517 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1606 687 482 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3114 3360 669 Size: 988 281 1176 246 3079 Fragments arranged by size: 3079 1176 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5770 HpaI GTT'AAC Cuts at: 3360 3360 Size: 5770 KpnI G_GTAC'C Cuts at: 3095 3095 Size: 5770 MfeI C'AATT_G Cuts at: 3369 3369 Size: 5770 MluI A'CGCG_T Cuts at: 5646 5646 Size: 5770 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4884 5068 1305 Size: 3579 184 2007 Fragments arranged by size: 3579 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2297 10 Size: 54 1425 343 465 3483 Fragments arranged by size: 3483 1425 465 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3728 4087 4246 518 Size: 648 2562 359 159 2042 Fragments arranged by size: 2562 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2104 2997 3186 3863 5011 5256 1606 Size: 498 893 189 677 1148 245 2120 Fragments arranged by size: 2120 1148 893 677 498 245 189 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4279 Fragments arranged by size: 4279 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5770 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5770 NotI GC'GGCC_GC Cuts at: 3217 3217 Size: 5770 NspI r_CATG'y Cuts at: 1132 1229 2368 3128 4673 1132 Size: 97 1139 760 1545 2229 Fragments arranged by size: 2229 1545 1139 760 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5770 PciI A'CATG_T Cuts at: 2364 4669 2364 Size: 2305 3465 PflMI CCAn_nnn'nTGG Cuts at: 1108 2226 1108 Size: 1118 4652 PfoI T'CCnGG_A Cuts at: 2164 2529 3054 2164 Size: 365 525 4880 Fragments arranged by size: 4880 525 365 PmeI GTTT'AAAC Cuts at: 3074 3074 Size: 5770 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3839 3871 5352 5384 3839 Size: 32 1481 32 4225 Fragments arranged by size: 4225 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5770 PsiI TTA'TAA Cuts at: 3340 3340 Size: 5770 PstI C_TGCA'G Cuts at: 830 3122 830 Size: 2292 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5770 PvuII CAG'CTG Cuts at: 2104 2997 2104 Size: 893 4877 SacI G_AGCT'C Cuts at: 2814 2814 Size: 5770 SalI G'TCGA_C Cuts at: 1936 3112 1936 Size: 1176 4594 SbfI CC_TGCA'GG Cuts at: 3122 3122 Size: 5770 ScaI AGT'ACT Cuts at: 4018 5730 4018 Size: 1712 4058 SfcI C'TryA_G Cuts at: 826 1045 3118 4253 4934 5125 826 Size: 219 2073 1135 681 191 1471 Fragments arranged by size: 2073 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5770 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5770 SmaI CCC'GGG Cuts at: 1872 3097 1872 Size: 1225 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2456 2972 3066 3874 4775 5037 Size: 197 926 513 516 94 808 901 262 Cuts at: 5037 820 Size: 1553 Fragments arranged by size: 1553 926 901 808 516 513 262 197 94 SnaBI TAC'GTA Cuts at: 493 3561 493 Size: 3068 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5770 SphI G_CATG'C Cuts at: 1132 3128 1132 Size: 1996 3774 SspI AAT'ATT Cuts at: 5 52 3694 5 Size: 47 3642 2081 Fragments arranged by size: 3642 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5770 StyI C'CwwG_G Cuts at: 513 1065 2004 3209 513 Size: 552 939 1205 3074 Fragments arranged by size: 3074 1205 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2617 3797 3814 3967 4152 4597 325 Size: 1255 1037 1180 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1180 1037 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2280 2523 2976 Size: 274 80 33 51 1443 303 243 453 Cuts at: 2976 4016 5728 96 Size: 1040 1712 138 Fragments arranged by size: 1712 1443 1040 453 303 274 243 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2108 2140 5730 5762 2108 Size: 32 3590 32 2116 Fragments arranged by size: 3590 2116 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5770 XbaI T'CTAG_A Cuts at: 3106 3106 Size: 5770 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2315 2011 Size: 304 5466 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5770 XmaI C'CCGG_G Cuts at: 1870 3095 1870 Size: 1225 4545 XmnI GAAnn'nnTTC Cuts at: 3899 3899 Size: 5770 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3578 276 Size: 53 83 186 936 2044 2468 Fragments arranged by size: 2468 2044 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI