(Circular) (Six-base) MAPSORT of: pFN21AB9834.seq Check: 9340 from: 1 to: 5743 pFN21AB9834 5743 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2170 2170 Size: 5743 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2952 3553 278 Size: 53 83 186 936 1416 601 2468 Fragments arranged by size: 2468 1416 936 601 186 83 53 AccI GT'mk_AC Cuts at: 1937 3086 1937 Size: 1149 4594 Acc65I G'GTAC_C Cuts at: 3064 3064 Size: 5743 AclI AA'CG_TT Cuts at: 1563 3870 4243 1563 Size: 2307 373 3063 Fragments arranged by size: 3063 2307 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2630 3806 5190 5458 1632 Size: 998 1176 1384 268 1917 Fragments arranged by size: 1917 1384 1176 998 268 AfeI AGC'GCT Cuts at: 2960 2960 Size: 5743 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5546 AflIII A'CryG_T Cuts at: 1203 4642 5619 1203 Size: 3439 977 1327 Fragments arranged by size: 3439 1327 977 AgeI A'CCGG_T Cuts at: 4574 4574 Size: 5743 AhdI GACnn_n'nnGTC Cuts at: 4472 4472 Size: 5743 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5743 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5743 AlwNI CAG_nnn'CTG Cuts at: 2200 3486 5058 2200 Size: 1286 1572 2885 Fragments arranged by size: 2885 1572 1286 ApaI G_GGCC'C Cuts at: 2134 2134 Size: 5743 ApaLI G'TGCA_C Cuts at: 2278 2597 3800 4956 2278 Size: 319 1203 1156 3065 Fragments arranged by size: 3065 1203 1156 319 ApoI r'AATT_y Cuts at: 1493 3052 3278 3430 5539 1493 Size: 1559 226 152 2109 1697 Fragments arranged by size: 2109 1697 1559 226 152 AseI AT'TA_AT Cuts at: 160 1032 4297 160 Size: 872 3265 1606 Fragments arranged by size: 3265 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5743 AvaI C'yCGr_G Cuts at: 1870 1943 2127 2930 3068 1870 Size: 73 184 803 138 4545 Fragments arranged by size: 4545 803 184 138 73 AvrII C'CTAG_G Cuts at: 2404 2404 Size: 5743 BamHI G'GATC_C Cuts at: 3073 3440 3073 Size: 367 5376 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2462 2833 3064 4519 618 Size: 325 217 627 675 371 231 1455 1842 Fragments arranged by size: 1842 1455 675 627 371 325 231 217 BanII G_rGCy'C Cuts at: 1391 2134 2325 2754 3062 1391 Size: 743 191 429 308 4072 Fragments arranged by size: 4072 743 429 308 191 BbeI G_GCGC'C Cuts at: 2466 2837 2466 Size: 371 5372 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2905 928 Size: 971 1006 3766 Fragments arranged by size: 3766 1006 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3934 3968 1903 Size: 34 50 34 1913 34 3678 Fragments arranged by size: 3678 1913 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3635 4845 3635 Size: 1210 4533 BclI T'GATC_A Cuts at: 1548 2628 1548 Size: 1080 4663 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2149 4354 136 Size: 107 122 71 631 605 477 2205 1525 Fragments arranged by size: 2205 1525 631 605 477 122 107 71 BglII A'GATC_T Cuts at: 2686 5738 2686 Size: 3052 2691 BlpI GC'TnA_GC Cuts at: 2319 3160 2319 Size: 841 4902 Bme1580I G_kGCm'C Cuts at: 1790 2134 2282 2601 3804 4960 1790 Size: 344 148 319 1203 1156 2573 Fragments arranged by size: 2573 1203 1156 344 319 148 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5743 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2182 3474 4432 Size: 416 53 83 399 24 765 1292 958 Cuts at: 4432 442 Size: 1753 Fragments arranged by size: 1753 1292 958 765 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5743 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2229 2532 2986 4403 1239 Size: 990 303 454 1417 2579 Fragments arranged by size: 2579 1417 990 454 303 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2525 3868 4733 5031 2525 Size: 1343 865 298 3237 Fragments arranged by size: 3237 1343 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2962 4406 882 Size: 617 1463 1444 2219 Fragments arranged by size: 2219 1463 1444 617 BsaAI yAC'GTr Cuts at: 493 1204 3534 493 Size: 711 2330 2702 Fragments arranged by size: 2702 2330 711 BsaBI GATnn'nnATC Cuts at: 5737 5737 Size: 5743 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2463 2774 2834 2949 Size: 53 83 186 936 930 311 60 115 Cuts at: 2949 3550 3932 4567 275 Size: 601 382 635 1451 Fragments arranged by size: 1451 936 930 635 601 382 311 186 115 83 60 53 BsaWI w'CCGG_w Cuts at: 2037 4176 4574 4848 4995 5406 2037 Size: 2139 398 274 147 411 2374 Fragments arranged by size: 2374 2139 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2168 2198 2754 2784 1178 Size: 30 165 30 765 30 556 30 4137 Fragments arranged by size: 4137 765 556 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5743 BseYI C'CCAG_C Cuts at: 2200 2972 4946 5442 2200 Size: 772 1974 496 2501 Fragments arranged by size: 2501 1974 772 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5170 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3193 3954 4982 1540 Size: 275 186 1192 761 1028 2301 Fragments arranged by size: 2301 1192 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2165 2282 2325 2601 2754 3804 3889 4960 2165 Size: 117 43 276 153 1050 85 1071 2948 Fragments arranged by size: 2948 1071 1050 276 153 117 85 43 BsmI GAATG_Cn' Cuts at: 3254 3347 3254 Size: 93 5650 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2134 2165 2282 2325 2601 2754 3062 Size: 399 344 31 117 43 276 153 308 Cuts at: 3062 3804 3889 4960 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 344 308 276 153 117 85 43 31 BspEI T'CCGG_A Cuts at: 2037 5406 2037 Size: 3369 2374 BspHI T'CATG_A Cuts at: 3632 5362 3632 Size: 1730 4013 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2104 2170 2895 3098 844 Size: 1260 66 725 203 3489 Fragments arranged by size: 3489 1260 725 203 66 BsrBI CCG'CTC Cuts at: 1394 1823 2851 3630 1394 Size: 429 1028 779 3507 Fragments arranged by size: 3507 1028 779 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4238 4412 65 Size: 1183 2990 174 1396 Fragments arranged by size: 2990 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2148 2468 4387 4574 1700 Size: 110 338 320 1919 187 2869 Fragments arranged by size: 2869 1919 338 320 187 110 BsrGI T'GTAC_A Cuts at: 96 2226 96 Size: 2130 3613 BssHII G'CGCG_C Cuts at: 1925 2994 1925 Size: 1069 4674 BssSI C'ACGA_G Cuts at: 3803 4815 3803 Size: 1012 4731 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5743 BstBI TT'CG_AA Cuts at: 4547 4547 Size: 5743 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5743 BstXI CCAn_nnnn'nTGG Cuts at: 2419 2419 Size: 5743 BstYI r'GATC_y Cuts at: 1972 2686 3013 3073 3440 3825 3842 5294 5380 Size: 714 327 60 367 385 17 1452 86 Cuts at: 5380 5392 5738 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 714 385 367 346 327 86 60 17 12 Bsu36I CC'TnA_GG Cuts at: 2478 2478 Size: 5743 BtgI C'CryG_G Cuts at: 513 1065 2135 2242 513 Size: 552 1070 107 4014 Fragments arranged by size: 4014 1070 552 107 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5004 Fragments arranged by size: 5004 683 56 BtsI GCAGTG_nn' Cuts at: 2500 3259 4053 4073 2500 Size: 759 794 20 4170 Fragments arranged by size: 4170 794 759 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5743 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5708 DraI TTT'AAA Cuts at: 3047 3047 Size: 5743 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4750 809 Size: 507 3434 1802 Fragments arranged by size: 3434 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2256 2470 3190 Size: 54 1425 50 275 18 426 214 720 Cuts at: 3190 4079 5708 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 720 426 275 214 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3190 1537 Size: 275 1378 4090 Fragments arranged by size: 4090 1378 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2127 2579 3673 1353 Size: 774 452 1094 3423 Fragments arranged by size: 3423 1094 774 452 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2320 4330 4704 4850 387 Size: 1064 138 731 2010 374 146 1280 Fragments arranged by size: 2010 1280 1064 731 374 146 138 EcoICRI GAG'CTC Cuts at: 2323 2752 2323 Size: 429 5314 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2229 2532 2630 2986 3806 4403 5190 Size: 393 597 303 98 356 820 597 787 Cuts at: 5190 5458 1239 Size: 268 1524 Fragments arranged by size: 1524 820 787 597 597 393 356 303 268 98 EcoNI CCTnn'n_nnAGG Cuts at: 2721 2721 Size: 5743 EcoO109I rG'GnC_Cy Cuts at: 2131 2131 Size: 5743 EcoRI G'AATT_C Cuts at: 3052 3430 3052 Size: 378 5365 FspI TGC'GCA Cuts at: 4249 4249 Size: 5743 HaeII r_GCGC'y Cuts at: 1678 2466 2837 2941 2962 4890 1678 Size: 788 371 104 21 1928 2531 Fragments arranged by size: 2531 1928 788 371 104 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3005 3037 4384 4416 4458 Size: 32 135 32 1433 32 1347 32 42 Cuts at: 4458 4490 1373 Size: 32 2626 Fragments arranged by size: 2626 1433 1347 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3087 3333 669 Size: 988 281 1149 246 3079 Fragments arranged by size: 3079 1149 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5743 HpaI GTT'AAC Cuts at: 3333 3333 Size: 5743 KasI G'GCGC_C Cuts at: 2462 2833 2462 Size: 371 5372 KpnI G_GTAC'C Cuts at: 3068 3068 Size: 5743 MfeI C'AATT_G Cuts at: 3342 3342 Size: 5743 MluI A'CGCG_T Cuts at: 5619 5619 Size: 5743 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2661 4857 5041 1305 Size: 1356 2196 184 2007 Fragments arranged by size: 2196 2007 1356 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2258 10 Size: 54 1425 343 426 3495 Fragments arranged by size: 3495 1425 426 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2417 3701 4060 4219 518 Size: 648 1251 1284 359 159 2042 Fragments arranged by size: 2042 1284 1251 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2137 2244 2906 3159 3836 4984 5229 1606 Size: 531 107 662 253 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 662 531 253 245 107 NaeI GCC'GGC Cuts at: 2150 2470 2150 Size: 320 5423 NarI GG'CG_CC Cuts at: 2463 2834 2463 Size: 371 5372 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5191 NdeI CA'TA_TG Cuts at: 387 387 Size: 5743 NgoMIV G'CCGG_C Cuts at: 2148 2468 2148 Size: 320 5423 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5743 NotI GC'GGCC_GC Cuts at: 3190 3190 Size: 5743 NspI r_CATG'y Cuts at: 1132 1229 3101 4646 1132 Size: 97 1872 1545 2229 Fragments arranged by size: 2229 1872 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5743 PciI A'CATG_T Cuts at: 4642 4642 Size: 5743 PflMI CCAn_nnn'nTGG Cuts at: 1108 2172 1108 Size: 1064 4679 PmeI GTTT'AAAC Cuts at: 3047 3047 Size: 5743 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3812 3844 5325 5357 3812 Size: 32 1481 32 4198 Fragments arranged by size: 4198 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5743 PsiI TTA'TAA Cuts at: 3313 3313 Size: 5743 PspOMI G'GGCC_C Cuts at: 2130 2130 Size: 5743 PstI C_TGCA'G Cuts at: 830 2078 2196 3095 830 Size: 1248 118 899 3478 Fragments arranged by size: 3478 1248 899 118 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5743 SacI G_AGCT'C Cuts at: 2325 2754 2325 Size: 429 5314 SacII CC_GC'GG Cuts at: 2138 2245 2138 Size: 107 5636 SalI G'TCGA_C Cuts at: 1936 3085 1936 Size: 1149 4594 SapI GCTCTTCn'nnn_ Cuts at: 2579 2579 Size: 5743 SbfI CC_TGCA'GG Cuts at: 3095 3095 Size: 5743 ScaI AGT'ACT Cuts at: 2546 3991 5703 2546 Size: 1445 1712 2586 Fragments arranged by size: 2586 1712 1445 SfcI C'TryA_G Cuts at: 826 1045 2074 2192 3091 4226 4907 5098 826 Size: 219 1029 118 899 1135 681 191 1471 Fragments arranged by size: 1471 1135 1029 899 681 219 191 118 SfoI GGC'GCC Cuts at: 2464 2835 2464 Size: 371 5372 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5743 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5743 SmaI CCC'GGG Cuts at: 1872 2932 3070 1872 Size: 1060 138 4545 Fragments arranged by size: 4545 1060 138 SmlI C'TyrA_G Cuts at: 820 1017 1943 2540 3847 4748 5010 820 Size: 197 926 597 1307 901 262 1553 Fragments arranged by size: 1553 1307 926 901 597 262 197 SnaBI TAC'GTA Cuts at: 493 3534 493 Size: 3041 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5743 SphI G_CATG'C Cuts at: 1132 3101 1132 Size: 1969 3774 SspI AAT'ATT Cuts at: 5 52 3667 5 Size: 47 3615 2081 Fragments arranged by size: 3615 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5743 StyI C'CwwG_G Cuts at: 513 1065 2404 2941 3182 513 Size: 552 1339 537 241 3074 Fragments arranged by size: 3074 1339 552 537 241 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2750 3770 3787 3940 4125 4570 325 Size: 1255 1170 1020 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1170 1020 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2226 2544 3989 Size: 274 80 33 51 1443 249 318 1445 Cuts at: 3989 5701 96 Size: 1712 138 Fragments arranged by size: 1712 1445 1443 318 274 249 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2168 2200 2754 2786 5703 5735 2168 Size: 32 554 32 2917 32 2176 Fragments arranged by size: 2917 2176 554 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5743 XbaI T'CTAG_A Cuts at: 3079 3079 Size: 5743 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2456 2456 Size: 5743 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5743 XmaI C'CCGG_G Cuts at: 1870 2930 3068 1870 Size: 1060 138 4545 Fragments arranged by size: 4545 1060 138 XmnI GAAnn'nnTTC Cuts at: 3872 3872 Size: 5743 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2950 3551 276 Size: 53 83 186 936 1416 601 2468 Fragments arranged by size: 2468 1416 936 601 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SanDI SexAI SfiI SrfI SwaI