(Circular) (Six-base) MAPSORT of: pFN21AB9829.seq Check: 4887 from: 1 to: 5725 pFN21AB9829 5725 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2329 2329 Size: 5725 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3535 278 Size: 53 83 186 936 1999 2468 Fragments arranged by size: 2468 1999 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2620 3068 1937 Size: 683 448 4594 Fragments arranged by size: 4594 683 448 Acc65I G'GTAC_C Cuts at: 3046 3046 Size: 5725 AclI AA'CG_TT Cuts at: 1563 3852 4225 1563 Size: 2289 373 3063 Fragments arranged by size: 3063 2289 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3788 5172 5440 1632 Size: 2156 1384 268 1917 Fragments arranged by size: 2156 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5528 AflIII A'CryG_T Cuts at: 1203 2282 4624 5601 1203 Size: 1079 2342 977 1327 Fragments arranged by size: 2342 1327 1079 977 AgeI A'CCGG_T Cuts at: 4556 4556 Size: 5725 AhdI GACnn_n'nnGTC Cuts at: 4454 4454 Size: 5725 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5725 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2162 2285 2361 1184 Size: 978 123 76 4548 Fragments arranged by size: 4548 978 123 76 AlwNI CAG_nnn'CTG Cuts at: 3468 5040 3468 Size: 1572 4153 ApaI G_GGCC'C Cuts at: 2177 2785 2177 Size: 608 5117 ApaLI G'TGCA_C Cuts at: 3782 4938 3782 Size: 1156 4569 ApoI r'AATT_y Cuts at: 1493 3034 3260 3412 5521 1493 Size: 1541 226 152 2109 1697 Fragments arranged by size: 2109 1697 1541 226 152 AseI AT'TA_AT Cuts at: 160 1032 4279 160 Size: 872 3247 1606 Fragments arranged by size: 3247 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5725 AvaI C'yCGr_G Cuts at: 1870 1943 2153 3050 1870 Size: 73 210 897 4545 Fragments arranged by size: 4545 897 210 73 BamHI G'GATC_C Cuts at: 3055 3422 3055 Size: 367 5358 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2393 2708 2746 3046 4501 Size: 325 217 627 606 315 38 300 1455 Cuts at: 4501 618 Size: 1842 Fragments arranged by size: 1842 1455 627 606 325 315 300 217 38 BanII G_rGCy'C Cuts at: 1391 2077 2087 2177 2785 3044 1391 Size: 686 10 90 608 259 4072 Fragments arranged by size: 4072 686 608 259 90 10 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2165 2285 2437 2533 928 Size: 971 266 120 152 96 4120 Fragments arranged by size: 4120 971 266 152 120 96 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3916 3950 1903 Size: 34 50 34 1895 34 3678 Fragments arranged by size: 3678 1895 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3617 4827 3617 Size: 1210 4515 BclI T'GATC_A Cuts at: 1548 2049 1548 Size: 501 5224 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2014 2433 4336 Size: 107 122 71 631 605 342 419 1903 Cuts at: 4336 136 Size: 1525 Fragments arranged by size: 1903 1525 631 605 419 342 122 107 71 BglII A'GATC_T Cuts at: 5720 5720 Size: 5725 BlpI GC'TnA_GC Cuts at: 3142 3142 Size: 5725 Bme1580I G_kGCm'C Cuts at: 1790 2177 2398 2713 2751 2785 3786 4942 1790 Size: 387 221 315 38 34 1001 1156 2573 Fragments arranged by size: 2573 1156 1001 387 315 221 38 34 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5725 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3456 4414 442 Size: 416 53 83 399 24 2039 958 1753 Fragments arranged by size: 2039 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5725 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2551 2551 Size: 5725 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2747 4385 1239 Size: 1508 1638 2579 Fragments arranged by size: 2579 1638 1508 Bpu10I CC'TnA_GC Cuts at: 2121 2349 2526 2121 Size: 228 177 5320 Fragments arranged by size: 5320 228 177 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3850 4715 5013 3850 Size: 865 298 4562 Fragments arranged by size: 4562 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4388 882 Size: 617 2889 2219 Fragments arranged by size: 2889 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2285 3516 493 Size: 711 1081 1231 2702 Fragments arranged by size: 2702 1231 1081 711 BsaBI GATnn'nnATC Cuts at: 5719 5719 Size: 5725 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3532 3914 4549 275 Size: 53 83 186 936 1999 382 635 1451 Fragments arranged by size: 1999 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4158 4556 4830 4977 5388 4158 Size: 398 274 147 411 4495 Fragments arranged by size: 4495 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2984 3014 1178 Size: 30 165 30 1581 30 3889 Fragments arranged by size: 3889 1581 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2579 1186 Size: 1393 4332 BseYI C'CCAG_C Cuts at: 2063 2125 2428 2459 4928 5424 2063 Size: 62 303 31 2469 496 2364 Fragments arranged by size: 2469 2364 496 303 62 31 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5152 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3175 3936 4964 1540 Size: 275 186 1174 761 1028 2301 Fragments arranged by size: 2301 1174 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2370 2470 2979 3786 3871 4942 2370 Size: 100 509 807 85 1071 3153 Fragments arranged by size: 3153 1071 807 509 100 85 BsmI GAATG_Cn' Cuts at: 2686 3236 3329 2686 Size: 550 93 5082 Fragments arranged by size: 5082 550 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2077 2087 2177 2370 2398 2470 2713 Size: 399 287 10 90 193 28 72 243 Cuts at: 2713 2751 2785 2979 3044 3786 3871 4942 1391 Size: 38 34 194 65 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 287 243 194 193 90 85 72 65 38 34 28 10 BspEI T'CCGG_A Cuts at: 5388 5388 Size: 5725 BspHI T'CATG_A Cuts at: 3614 5344 3614 Size: 1730 3995 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2329 3080 844 Size: 1485 751 3489 Fragments arranged by size: 3489 1485 751 BsrBI CCG'CTC Cuts at: 1394 1823 3612 1394 Size: 429 1789 3507 Fragments arranged by size: 3507 1789 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2669 4220 4394 65 Size: 1183 1421 1551 174 1396 Fragments arranged by size: 1551 1421 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2334 4369 4556 1700 Size: 110 524 2035 187 2869 Fragments arranged by size: 2869 2035 524 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5725 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5725 BssSI C'ACGA_G Cuts at: 3785 4797 3785 Size: 1012 4713 BstAPI GCAn_nnn'nTGC Cuts at: 794 2671 794 Size: 1877 3848 BstBI TT'CG_AA Cuts at: 4529 4529 Size: 5725 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5725 BstXI CCAn_nnnn'nTGG Cuts at: 2641 2641 Size: 5725 BstYI r'GATC_y Cuts at: 1972 3055 3422 3807 3824 5276 5362 5374 5720 Size: 1083 367 385 17 1452 86 12 346 Cuts at: 5720 1972 Size: 1977 Fragments arranged by size: 1977 1452 1083 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2178 2178 Size: 5725 BtgI C'CryG_G Cuts at: 513 1065 2055 513 Size: 552 990 4183 Fragments arranged by size: 4183 990 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4986 Fragments arranged by size: 4986 683 56 BtsI GCAGTG_nn' Cuts at: 2221 3241 4035 4055 2221 Size: 1020 794 20 3891 Fragments arranged by size: 3891 1020 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5725 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5690 DraI TTT'AAA Cuts at: 3029 3029 Size: 5725 DraIII CAC_nnn'GTG Cuts at: 2474 2778 2474 Size: 304 5421 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4732 809 Size: 507 3416 1802 Fragments arranged by size: 3416 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2015 3172 4061 Size: 54 1425 50 275 18 185 1157 889 Cuts at: 4061 5690 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1157 889 275 185 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3172 1537 Size: 275 1360 4090 Fragments arranged by size: 4090 1360 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2940 3655 1353 Size: 1587 715 3423 Fragments arranged by size: 3423 1587 715 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4312 4686 4832 387 Size: 1064 138 2723 374 146 1280 Fragments arranged by size: 2723 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2747 3788 4385 5172 5440 1239 Size: 393 1115 1041 597 787 268 1524 Fragments arranged by size: 1524 1115 1041 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2174 2782 2915 2950 2174 Size: 608 133 35 4949 Fragments arranged by size: 4949 608 133 35 EcoRI G'AATT_C Cuts at: 3034 3412 3034 Size: 378 5347 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2969 2969 Size: 5725 FspI TGC'GCA Cuts at: 4231 4231 Size: 5725 HaeII r_GCGC'y Cuts at: 1678 2307 2490 2992 4872 1678 Size: 629 183 502 1880 2531 Fragments arranged by size: 2531 1880 629 502 183 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4366 4398 4440 4472 1373 Size: 32 135 32 2794 32 42 32 2626 Fragments arranged by size: 2794 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2621 3069 3315 669 Size: 988 281 683 448 246 3079 Fragments arranged by size: 3079 988 683 448 281 246 HindIII A'AGCT_T Cuts at: 748 2636 748 Size: 1888 3837 HpaI GTT'AAC Cuts at: 3315 3315 Size: 5725 KpnI G_GTAC'C Cuts at: 3050 3050 Size: 5725 MfeI C'AATT_G Cuts at: 3324 3324 Size: 5725 MluI A'CGCG_T Cuts at: 5601 5601 Size: 5725 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2892 4839 5023 1305 Size: 1587 1947 184 2007 Fragments arranged by size: 2007 1947 1587 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2017 10 Size: 54 1425 343 185 3718 Fragments arranged by size: 3718 1425 343 185 54 MslI CAynn'nnrTG Cuts at: 518 1166 3683 4042 4201 518 Size: 648 2517 359 159 2042 Fragments arranged by size: 2517 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2063 2269 2404 2432 2463 3141 3818 4966 Size: 457 206 135 28 31 678 677 1148 Cuts at: 4966 5211 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 678 677 457 245 206 135 31 28 NcoI C'CATG_G Cuts at: 513 1065 2055 513 Size: 552 990 4183 Fragments arranged by size: 4183 990 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5725 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5725 NotI GC'GGCC_GC Cuts at: 3172 3172 Size: 5725 NspI r_CATG'y Cuts at: 1132 1229 2887 2912 3083 4628 1132 Size: 97 1658 25 171 1545 2229 Fragments arranged by size: 2229 1658 1545 171 97 25 PasI CC'CwG_GG Cuts at: 1348 2569 2809 1348 Size: 1221 240 4264 Fragments arranged by size: 4264 1221 240 PciI A'CATG_T Cuts at: 4624 4624 Size: 5725 PflMI CCAn_nnn'nTGG Cuts at: 1108 2778 1108 Size: 1670 4055 PfoI T'CCnGG_A Cuts at: 2982 2982 Size: 5725 PmeI GTTT'AAAC Cuts at: 3029 3029 Size: 5725 PmlI CAC'GTG Cuts at: 2285 2285 Size: 5725 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3794 3826 5307 5339 3794 Size: 32 1481 32 4180 Fragments arranged by size: 4180 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5725 PsiI TTA'TAA Cuts at: 3295 3295 Size: 5725 PspOMI G'GGCC_C Cuts at: 2173 2781 2173 Size: 608 5117 PstI C_TGCA'G Cuts at: 830 2551 2563 3077 830 Size: 1721 12 514 3478 Fragments arranged by size: 3478 1721 514 12 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5725 PvuII CAG'CTG Cuts at: 2063 2404 2432 2063 Size: 341 28 5356 Fragments arranged by size: 5356 341 28 SalI G'TCGA_C Cuts at: 1936 2619 3067 1936 Size: 683 448 4594 Fragments arranged by size: 4594 683 448 SbfI CC_TGCA'GG Cuts at: 2551 3077 2551 Size: 526 5199 ScaI AGT'ACT Cuts at: 3973 5685 3973 Size: 1712 4013 SexAI A'CCwGG_T Cuts at: 2389 2871 2389 Size: 482 5243 SfcI C'TryA_G Cuts at: 826 1045 2547 2559 2598 2692 3073 4208 4889 Size: 219 1502 12 39 94 381 1135 681 Cuts at: 4889 5080 826 Size: 191 1471 Fragments arranged by size: 1502 1471 1135 681 381 219 191 94 39 12 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5725 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5725 SmaI CCC'GGG Cuts at: 1872 3052 1872 Size: 1180 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2153 3829 4730 4992 820 Size: 197 926 210 1676 901 262 1553 Fragments arranged by size: 1676 1553 926 901 262 210 197 SnaBI TAC'GTA Cuts at: 493 3516 493 Size: 3023 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5725 SphI G_CATG'C Cuts at: 1132 2912 3083 1132 Size: 1780 171 3774 Fragments arranged by size: 3774 1780 171 SspI AAT'ATT Cuts at: 5 52 3649 5 Size: 47 3597 2081 Fragments arranged by size: 3597 2081 47 StuI AGG'CCT Cuts at: 1517 2524 1517 Size: 1007 4718 StyI C'CwwG_G Cuts at: 513 1065 2055 3164 513 Size: 552 990 1109 3074 Fragments arranged by size: 3074 1109 990 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2696 2809 2837 3752 3769 3922 4107 Size: 1255 1116 113 28 915 17 153 185 Cuts at: 4107 4552 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1116 915 445 185 153 113 28 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3971 5683 96 Size: 274 80 33 51 1443 1994 1712 138 Fragments arranged by size: 1994 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2873 2905 2984 3016 5685 5717 2873 Size: 32 79 32 2669 32 2881 Fragments arranged by size: 2881 2669 79 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2873 1316 Size: 1557 4168 XbaI T'CTAG_A Cuts at: 3061 3061 Size: 5725 XhoI C'TCGA_G Cuts at: 1943 2153 1943 Size: 210 5515 XmaI C'CCGG_G Cuts at: 1870 3050 1870 Size: 1180 4545 XmnI GAAnn'nnTTC Cuts at: 3854 3854 Size: 5725 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3533 276 Size: 53 83 186 936 1999 2468 Fragments arranged by size: 2468 1999 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI