(Circular) (Six-base) MAPSORT of: pFN21AB9825.seq Check: 6815 from: 1 to: 5656 pFN21AB9825 5656 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2555 2555 Size: 5656 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3466 278 Size: 53 83 186 936 1930 2468 Fragments arranged by size: 2468 1930 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2999 1937 Size: 1062 4594 Acc65I G'GTAC_C Cuts at: 2788 2977 2788 Size: 189 5467 AclI AA'CG_TT Cuts at: 1563 3783 4156 1563 Size: 2220 373 3063 Fragments arranged by size: 3063 2220 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2501 3719 5103 5371 1632 Size: 869 1218 1384 268 1917 Fragments arranged by size: 1917 1384 1218 869 268 AfeI AGC'GCT Cuts at: 2441 2441 Size: 5656 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5459 AflIII A'CryG_T Cuts at: 1203 4555 5532 1203 Size: 3352 977 1327 Fragments arranged by size: 3352 1327 977 AgeI A'CCGG_T Cuts at: 4487 4487 Size: 5656 AhdI GACnn_n'nnGTC Cuts at: 2311 4385 2311 Size: 2074 3582 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5656 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2133 1184 Size: 949 4707 AlwNI CAG_nnn'CTG Cuts at: 3399 4971 3399 Size: 1572 4084 ApaLI G'TGCA_C Cuts at: 3713 4869 3713 Size: 1156 4500 ApoI r'AATT_y Cuts at: 1493 2965 3191 3343 5452 1493 Size: 1472 226 152 2109 1697 Fragments arranged by size: 2109 1697 1472 226 152 AseI AT'TA_AT Cuts at: 160 1032 4210 160 Size: 872 3178 1606 Fragments arranged by size: 3178 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5656 AvaI C'yCGr_G Cuts at: 1870 1943 2981 1870 Size: 73 1038 4545 Fragments arranged by size: 4545 1038 73 BamHI G'GATC_C Cuts at: 2986 3353 2986 Size: 367 5289 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2150 2788 2977 4432 618 Size: 325 217 627 363 638 189 1455 1842 Fragments arranged by size: 1842 1455 638 627 363 325 217 189 BanII G_rGCy'C Cuts at: 1391 2733 2839 2975 1391 Size: 1342 106 136 4072 Fragments arranged by size: 4072 1342 136 106 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2001 928 Size: 971 102 4583 Fragments arranged by size: 4583 971 102 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3847 3881 1903 Size: 34 1910 34 3678 Fragments arranged by size: 3678 1910 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3548 4758 3548 Size: 1210 4446 BclI T'GATC_A Cuts at: 1548 1548 Size: 5656 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4267 136 Size: 107 122 71 631 605 2595 1525 Fragments arranged by size: 2595 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2589 5651 2589 Size: 3062 2594 BlpI GC'TnA_GC Cuts at: 3073 3073 Size: 5656 Bme1580I G_kGCm'C Cuts at: 1790 2630 3717 4873 1790 Size: 840 1087 1156 2573 Fragments arranged by size: 2573 1156 1087 840 BmgBI CAC'GTC Cuts at: 1318 2503 1318 Size: 1185 4471 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3387 4345 442 Size: 416 53 83 399 24 1970 958 1753 Fragments arranged by size: 1970 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5656 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2348 2348 Size: 5656 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2177 2266 2435 2795 2888 4316 1239 Size: 938 89 169 360 93 1428 2579 Fragments arranged by size: 2579 1428 938 360 169 93 89 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3781 4646 4944 3781 Size: 865 298 4493 Fragments arranged by size: 4493 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4319 882 Size: 617 2820 2219 Fragments arranged by size: 2820 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3447 493 Size: 711 2243 2702 Fragments arranged by size: 2702 2243 711 BsaBI GATnn'nnATC Cuts at: 5650 5650 Size: 5656 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3463 3845 4480 275 Size: 53 83 186 936 1930 382 635 1451 Fragments arranged by size: 1930 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4089 4487 4761 4908 5319 4089 Size: 398 274 147 411 4426 Fragments arranged by size: 4426 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5431 Fragments arranged by size: 5431 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2116 2119 2120 2122 2127 2297 2675 2690 Size: 930 3 1 2 5 170 378 15 Cuts at: 2690 2954 1186 Size: 264 3888 Fragments arranged by size: 3888 930 378 264 170 15 5 3 2 1 BseYI C'CCAG_C Cuts at: 2575 2680 4859 5355 2575 Size: 105 2179 496 2876 Fragments arranged by size: 2876 2179 496 105 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2780 1163 Size: 573 1044 4039 Fragments arranged by size: 4039 1044 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3106 3867 4895 1540 Size: 275 186 1105 761 1028 2301 Fragments arranged by size: 2301 1105 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2715 3717 3802 4873 2715 Size: 1002 85 1071 3498 Fragments arranged by size: 3498 1071 1002 85 BsmI GAATG_Cn' Cuts at: 3167 3260 3167 Size: 93 5563 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2630 2715 2733 2839 2975 3717 3802 Size: 399 840 85 18 106 136 742 85 Cuts at: 3802 4873 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 840 742 399 136 106 85 85 18 BspEI T'CCGG_A Cuts at: 5319 5319 Size: 5656 BspHI T'CATG_A Cuts at: 3545 5275 3545 Size: 1730 3926 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2174 2555 2776 3011 844 Size: 1330 381 221 235 3489 Fragments arranged by size: 3489 1330 381 235 221 BsrBI CCG'CTC Cuts at: 1394 1823 3543 1394 Size: 429 1720 3507 Fragments arranged by size: 3507 1720 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2643 2901 4151 4325 65 Size: 1183 1395 258 1250 174 1396 Fragments arranged by size: 1396 1395 1250 1183 258 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2781 4300 4487 1700 Size: 110 971 1519 187 2869 Fragments arranged by size: 2869 1519 971 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5656 BssHII G'CGCG_C Cuts at: 1925 2807 1925 Size: 882 4774 BssSI C'ACGA_G Cuts at: 3716 4728 3716 Size: 1012 4644 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5656 BstBI TT'CG_AA Cuts at: 4460 4460 Size: 5656 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5656 BstXI CCAn_nnnn'nTGG Cuts at: 2392 2392 Size: 5656 BstYI r'GATC_y Cuts at: 1972 2589 2986 3353 3738 3755 5207 5293 5305 Size: 617 397 367 385 17 1452 86 12 Cuts at: 5305 5651 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 617 397 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2040 2202 2040 Size: 162 5494 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5104 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4917 Fragments arranged by size: 4917 683 56 BtsI GCAGTG_nn' Cuts at: 2029 3172 3966 3986 2029 Size: 1143 794 20 3699 Fragments arranged by size: 3699 1143 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5656 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5621 DraI TTT'AAA Cuts at: 2960 2960 Size: 5656 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4663 809 Size: 507 3347 1802 Fragments arranged by size: 3347 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3103 3992 5621 Size: 54 1425 50 275 18 1273 889 1629 Cuts at: 5621 8 Size: 43 Fragments arranged by size: 1629 1425 1273 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3103 1537 Size: 275 1291 4090 Fragments arranged by size: 4090 1291 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2015 2058 2274 2940 3586 1353 Size: 662 43 216 666 646 3423 Fragments arranged by size: 3423 666 662 646 216 43 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4243 4617 4763 387 Size: 1064 138 2654 374 146 1280 Fragments arranged by size: 2654 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2177 2266 2435 2501 2795 2888 3719 Size: 393 545 89 169 66 294 93 831 Cuts at: 3719 4316 5103 5371 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 831 787 597 545 393 294 268 169 93 89 66 EcoNI CCTnn'n_nnAGG Cuts at: 2043 2043 Size: 5656 EcoO109I rG'GnC_Cy Cuts at: 2068 2186 2068 Size: 118 5538 EcoRI G'AATT_C Cuts at: 2965 3343 2965 Size: 378 5278 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2461 2506 2461 Size: 45 5611 FspI TGC'GCA Cuts at: 2088 4162 2088 Size: 2074 3582 HaeII r_GCGC'y Cuts at: 1678 2443 2655 4803 1678 Size: 765 212 2148 2531 Fragments arranged by size: 2531 2148 765 212 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2297 2329 2639 2671 4297 Size: 32 135 32 725 32 310 32 1626 Cuts at: 4297 4329 4371 4403 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1626 725 310 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3000 3246 669 Size: 988 281 1062 246 3079 Fragments arranged by size: 3079 1062 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5656 HpaI GTT'AAC Cuts at: 3246 3246 Size: 5656 KpnI G_GTAC'C Cuts at: 2792 2981 2792 Size: 189 5467 MfeI C'AATT_G Cuts at: 2631 3255 2631 Size: 624 5032 MluI A'CGCG_T Cuts at: 5532 5532 Size: 5656 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4770 4954 1305 Size: 3465 184 2007 Fragments arranged by size: 3465 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3834 Fragments arranged by size: 3834 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2501 3614 3973 4132 518 Size: 648 1335 1113 359 159 2042 Fragments arranged by size: 2042 1335 1113 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3072 3749 4897 5142 1606 Size: 1466 677 1148 245 2120 Fragments arranged by size: 2120 1466 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5104 NdeI CA'TA_TG Cuts at: 387 387 Size: 5656 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5656 NotI GC'GGCC_GC Cuts at: 3103 3103 Size: 5656 NspI r_CATG'y Cuts at: 1132 1229 2876 3014 4559 1132 Size: 97 1647 138 1545 2229 Fragments arranged by size: 2229 1647 1545 138 97 PasI CC'CwG_GG Cuts at: 1348 2071 1348 Size: 723 4933 PciI A'CATG_T Cuts at: 4555 4555 Size: 5656 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5656 PfoI T'CCnGG_A Cuts at: 2865 2865 Size: 5656 PmeI GTTT'AAAC Cuts at: 2960 2960 Size: 5656 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2303 2335 3725 3757 5238 5270 2303 Size: 32 1390 32 1481 32 2689 Fragments arranged by size: 2689 1481 1390 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2186 2186 Size: 5656 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5656 PsiI TTA'TAA Cuts at: 3226 3226 Size: 5656 PstI C_TGCA'G Cuts at: 830 3008 830 Size: 2178 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5656 SalI G'TCGA_C Cuts at: 1936 2998 1936 Size: 1062 4594 SbfI CC_TGCA'GG Cuts at: 3008 3008 Size: 5656 ScaI AGT'ACT Cuts at: 3904 5616 3904 Size: 1712 3944 SfcI C'TryA_G Cuts at: 826 1045 3004 4139 4820 5011 826 Size: 219 1959 1135 681 191 1471 Fragments arranged by size: 1959 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5656 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5656 SmaI CCC'GGG Cuts at: 1872 2983 1872 Size: 1111 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3760 4661 4923 820 Size: 197 926 1817 901 262 1553 Fragments arranged by size: 1817 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3447 493 Size: 2954 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5656 SphI G_CATG'C Cuts at: 1132 3014 1132 Size: 1882 3774 SspI AAT'ATT Cuts at: 5 52 3580 5 Size: 47 3528 2081 Fragments arranged by size: 3528 2081 47 StuI AGG'CCT Cuts at: 1517 2607 1517 Size: 1090 4566 StyI C'CwwG_G Cuts at: 513 1065 2385 2831 3095 513 Size: 552 1320 446 264 3074 Fragments arranged by size: 3074 1320 552 446 264 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2072 2672 2746 2762 3683 3700 3853 Size: 1255 492 600 74 16 921 17 153 Cuts at: 3853 4038 4483 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 921 600 492 445 185 153 74 17 16 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3902 5614 96 Size: 274 80 33 51 1443 1925 1712 138 Fragments arranged by size: 1925 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5616 5648 5616 Size: 32 5624 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5656 XbaI T'CTAG_A Cuts at: 2992 2992 Size: 5656 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5656 XmaI C'CCGG_G Cuts at: 1870 2981 1870 Size: 1111 4545 XmnI GAAnn'nnTTC Cuts at: 3785 3785 Size: 5656 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3464 276 Size: 53 83 186 936 1930 2468 Fragments arranged by size: 2468 1930 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI