(Circular) (Six-base) MAPSORT of: pFN21AB9821.seq Check: 6474 from: 1 to: 5641 pFN21AB9821 5641 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3451 278 Size: 53 83 186 936 1915 2468 Fragments arranged by size: 2468 1915 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2984 1937 Size: 1047 4594 Acc65I G'GTAC_C Cuts at: 2962 2962 Size: 5641 AclI AA'CG_TT Cuts at: 1563 3768 4141 1563 Size: 2205 373 3063 Fragments arranged by size: 3063 2205 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3704 5088 5356 1632 Size: 2072 1384 268 1917 Fragments arranged by size: 2072 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5444 AflIII A'CryG_T Cuts at: 1203 4540 5517 1203 Size: 3337 977 1327 Fragments arranged by size: 3337 1327 977 AgeI A'CCGG_T Cuts at: 4472 4472 Size: 5641 AhdI GACnn_n'nnGTC Cuts at: 4370 4370 Size: 5641 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5641 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5641 AlwNI CAG_nnn'CTG Cuts at: 2790 3384 4956 2790 Size: 594 1572 3475 Fragments arranged by size: 3475 1572 594 ApaLI G'TGCA_C Cuts at: 3698 4854 3698 Size: 1156 4485 ApoI r'AATT_y Cuts at: 1493 2950 3176 3328 5437 1493 Size: 1457 226 152 2109 1697 Fragments arranged by size: 2109 1697 1457 226 152 AseI AT'TA_AT Cuts at: 160 1032 4195 160 Size: 872 3163 1606 Fragments arranged by size: 3163 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5641 AvaI C'yCGr_G Cuts at: 1870 1943 2458 2966 1870 Size: 73 515 508 4545 Fragments arranged by size: 4545 515 508 73 BamHI G'GATC_C Cuts at: 2971 3338 2971 Size: 367 5274 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2920 2962 4417 618 Size: 325 217 627 1133 42 1455 1842 Fragments arranged by size: 1842 1455 1133 627 325 217 42 BanII G_rGCy'C Cuts at: 1391 2207 2255 2306 2753 2813 2960 1391 Size: 816 48 51 447 60 147 4072 Fragments arranged by size: 4072 816 447 147 60 51 48 BbeI G_GCGC'C Cuts at: 2924 2924 Size: 5641 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2063 928 Size: 971 164 4506 Fragments arranged by size: 4506 971 164 BbvCI CC'TCA_GC Cuts at: 2034 2034 Size: 5641 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3832 3866 1903 Size: 34 1895 34 3678 Fragments arranged by size: 3678 1895 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3533 4743 3533 Size: 1210 4431 BclI T'GATC_A Cuts at: 1548 1548 Size: 5641 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2929 4252 136 Size: 107 122 71 631 605 1257 1323 1525 Fragments arranged by size: 1525 1323 1257 631 605 122 107 71 BglII A'GATC_T Cuts at: 5636 5636 Size: 5641 BlpI GC'TnA_GC Cuts at: 3058 3058 Size: 5641 Bme1580I G_kGCm'C Cuts at: 1790 3702 4858 1790 Size: 1912 1156 2573 Fragments arranged by size: 2573 1912 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5641 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2036 3372 4330 Size: 416 53 83 399 24 619 1336 958 Cuts at: 4330 442 Size: 1753 Fragments arranged by size: 1753 1336 958 619 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5641 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2302 2377 2521 2713 2856 4301 1239 Size: 1063 75 144 192 143 1445 2579 Fragments arranged by size: 2579 1445 1063 192 144 143 75 Bpu10I CC'TnA_GC Cuts at: 2034 2128 2034 Size: 94 5547 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3766 4631 4929 3766 Size: 865 298 4478 Fragments arranged by size: 4478 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4304 882 Size: 617 2805 2219 Fragments arranged by size: 2805 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3432 493 Size: 711 2228 2702 Fragments arranged by size: 2702 2228 711 BsaBI GATnn'nnATC Cuts at: 5635 5635 Size: 5641 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2493 2886 2921 3448 Size: 53 83 186 936 960 393 35 527 Cuts at: 3448 3830 4465 275 Size: 382 635 1451 Fragments arranged by size: 1451 960 936 635 527 393 382 186 83 53 35 BsaWI w'CCGG_w Cuts at: 2255 2276 2351 4074 4472 4746 4893 5304 2255 Size: 21 75 1723 398 274 147 411 2592 Fragments arranged by size: 2592 1723 411 398 274 147 75 21 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5416 Fragments arranged by size: 5416 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2197 2281 2338 2356 2401 2428 2500 2593 Size: 1011 84 57 18 45 27 72 93 Cuts at: 2593 2623 2761 2845 1186 Size: 30 138 84 3982 Fragments arranged by size: 3982 1011 138 93 84 84 72 57 45 30 27 18 BseYI C'CCAG_C Cuts at: 2667 2907 4844 5340 2667 Size: 240 1937 496 2968 Fragments arranged by size: 2968 1937 496 240 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5068 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3091 3852 4880 1540 Size: 275 186 1090 761 1028 2301 Fragments arranged by size: 2301 1090 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2753 3702 3787 4858 2753 Size: 949 85 1071 3536 Fragments arranged by size: 3536 1071 949 85 BsmI GAATG_Cn' Cuts at: 3152 3245 3152 Size: 93 5548 BsmBI CGTCTCn'nnnn_ Cuts at: 2500 2635 2500 Size: 135 5506 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2207 2255 2306 2753 2813 2960 3702 Size: 399 417 48 51 447 60 147 742 Cuts at: 3702 3787 4858 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 447 417 399 147 85 60 51 48 BspEI T'CCGG_A Cuts at: 2276 5304 2276 Size: 3028 2613 BspHI T'CATG_A Cuts at: 3530 5260 3530 Size: 1730 3911 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2632 2996 844 Size: 1788 364 3489 Fragments arranged by size: 3489 1788 364 BsrBI CCG'CTC Cuts at: 1394 1823 3528 1394 Size: 429 1705 3507 Fragments arranged by size: 3507 1705 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4136 4310 65 Size: 1183 2888 174 1396 Fragments arranged by size: 2888 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4285 4472 1700 Size: 110 2475 187 2869 Fragments arranged by size: 2869 2475 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5641 BssHII G'CGCG_C Cuts at: 1925 2661 1925 Size: 736 4905 BssSI C'ACGA_G Cuts at: 2656 3701 4713 2656 Size: 1045 1012 3584 Fragments arranged by size: 3584 1045 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2790 794 Size: 1996 3645 BstBI TT'CG_AA Cuts at: 4445 4445 Size: 5641 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5641 BstYI r'GATC_y Cuts at: 1972 2971 3338 3723 3740 5192 5278 5290 5636 Size: 999 367 385 17 1452 86 12 346 Cuts at: 5636 1972 Size: 1977 Fragments arranged by size: 1977 1452 999 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2859 513 Size: 552 1794 3295 Fragments arranged by size: 3295 1794 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4902 Fragments arranged by size: 4902 683 56 BtsI GCAGTG_nn' Cuts at: 3157 3951 3971 3157 Size: 794 20 4827 Fragments arranged by size: 4827 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5641 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5606 DraI TTT'AAA Cuts at: 2945 2945 Size: 5641 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4648 809 Size: 507 3332 1802 Fragments arranged by size: 3332 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2930 3088 3977 Size: 54 1425 50 275 18 1100 158 889 Cuts at: 3977 5606 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1100 889 275 158 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3088 1537 Size: 275 1276 4090 Fragments arranged by size: 4090 1276 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3571 1353 Size: 2218 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2610 2727 4228 4602 4748 387 Size: 1064 138 1021 117 1501 374 146 1280 Fragments arranged by size: 1501 1280 1064 1021 374 146 138 117 EcoICRI GAG'CTC Cuts at: 2751 2751 Size: 5641 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2302 2377 2521 2713 2856 3704 4301 Size: 393 670 75 144 192 143 848 597 Cuts at: 4301 5088 5356 1239 Size: 787 268 1524 Fragments arranged by size: 1524 848 787 670 597 393 268 192 144 143 75 EcoRI G'AATT_C Cuts at: 2950 3328 2950 Size: 378 5263 FspI TGC'GCA Cuts at: 4147 4147 Size: 5641 HaeII r_GCGC'y Cuts at: 1678 2924 4788 1678 Size: 1246 1864 2531 Fragments arranged by size: 2531 1864 1246 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4282 4314 4356 4388 1373 Size: 32 135 32 2710 32 42 32 2626 Fragments arranged by size: 2710 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2985 3231 669 Size: 988 281 1047 246 3079 Fragments arranged by size: 3079 1047 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5641 HpaI GTT'AAC Cuts at: 3231 3231 Size: 5641 KasI G'GCGC_C Cuts at: 2920 2920 Size: 5641 KpnI G_GTAC'C Cuts at: 2966 2966 Size: 5641 MfeI C'AATT_G Cuts at: 3240 3240 Size: 5641 MluI A'CGCG_T Cuts at: 5517 5517 Size: 5641 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4755 4939 1305 Size: 3450 184 2007 Fragments arranged by size: 3450 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3819 Fragments arranged by size: 3819 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3599 3958 4117 518 Size: 648 2433 359 159 2042 Fragments arranged by size: 2433 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2091 2145 2322 2397 2541 2616 2709 2733 Size: 485 54 177 75 144 75 93 24 Cuts at: 2733 2787 3057 3734 4882 5127 1606 Size: 54 270 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 485 270 245 177 144 93 75 75 54 54 24 NarI GG'CG_CC Cuts at: 2921 2921 Size: 5641 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5089 NdeI CA'TA_TG Cuts at: 387 387 Size: 5641 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5641 NotI GC'GGCC_GC Cuts at: 3088 3088 Size: 5641 NspI r_CATG'y Cuts at: 1132 1229 2999 4544 1132 Size: 97 1770 1545 2229 Fragments arranged by size: 2229 1770 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5641 PciI A'CATG_T Cuts at: 4540 4540 Size: 5641 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5641 PfoI T'CCnGG_A Cuts at: 2774 2870 2774 Size: 96 5545 PmeI GTTT'AAAC Cuts at: 2945 2945 Size: 5641 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3710 3742 5223 5255 3710 Size: 32 1481 32 4096 Fragments arranged by size: 4096 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5641 PsiI TTA'TAA Cuts at: 3211 3211 Size: 5641 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2131 2163 2131 Size: 32 5609 PstI C_TGCA'G Cuts at: 830 2993 830 Size: 2163 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5641 SacI G_AGCT'C Cuts at: 2753 2753 Size: 5641 SalI G'TCGA_C Cuts at: 1936 2983 1936 Size: 1047 4594 SbfI CC_TGCA'GG Cuts at: 2993 2993 Size: 5641 ScaI AGT'ACT Cuts at: 2273 3889 5601 2273 Size: 1616 1712 2313 Fragments arranged by size: 2313 1712 1616 SfcI C'TryA_G Cuts at: 826 1045 2989 4124 4805 4996 826 Size: 219 1944 1135 681 191 1471 Fragments arranged by size: 1944 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2922 2922 Size: 5641 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5641 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5641 SmaI CCC'GGG Cuts at: 1872 2968 1872 Size: 1096 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3745 4646 4908 820 Size: 197 926 1802 901 262 1553 Fragments arranged by size: 1802 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3432 493 Size: 2939 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5641 SphI G_CATG'C Cuts at: 1132 2999 1132 Size: 1867 3774 SspI AAT'ATT Cuts at: 5 52 3565 5 Size: 47 3513 2081 Fragments arranged by size: 3513 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5641 StyI C'CwwG_G Cuts at: 513 1065 3080 513 Size: 552 2015 3074 Fragments arranged by size: 3074 2015 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2852 3668 3685 3838 4023 4468 325 Size: 1255 1272 816 17 153 185 445 1498 Fragments arranged by size: 1498 1272 1255 816 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2271 3887 5599 Size: 274 80 33 51 1443 294 1616 1712 Cuts at: 5599 96 Size: 138 Fragments arranged by size: 1712 1616 1443 294 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5601 5633 5601 Size: 32 5609 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5641 XbaI T'CTAG_A Cuts at: 2977 2977 Size: 5641 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5641 XmaI C'CCGG_G Cuts at: 1870 2966 1870 Size: 1096 4545 XmnI GAAnn'nnTTC Cuts at: 3770 3770 Size: 5641 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3449 276 Size: 53 83 186 936 1915 2468 Fragments arranged by size: 2468 1915 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PvuII RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI