Restriction map for
pFN21AB9820
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflIII AgeI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AvaI
AviII AvrII BamHI BbeI BbrPI
BbsI BcgI BclI BcnI BlnI
BpuAI BsaAI BsaBI BsaHI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I Cac8I CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HindIII
HpaI KasI KpnI KspI MaeII
MamI MluI MslI MspA1I MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfaNI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI TthHB8I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccIII 696 TCCGGA T^CCGGA
AcsI 25 GAATTT [GA]^AATT[CT]
AfaI 101 GTAC GT^AC
AflII 769 CTTAAG C^TTAAG
AhdI 349 GACGAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 306 GGATC GGATC(4/5)
ApoI 25 GAATTT [GA]^AATT[CT]
AspEI 349 GACGAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 191 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 45 TGGCCA TGG^CCA
BanII 457 GAGCTC G[GA]GC[CT]^C
BciVI 434 GTATCC GTATCC(6/5)
BfaI 607 CTAG C^TAG
BfrI 769 CTTAAG C^TTAAG
BglI 263 GCCCCCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 650 AGATCT A^GATCT
BlpI 124 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 124 GCTGAGC GC^T[ACGT]AGC
BsaI 189 GAGACC GGTCTC(1/5)
BsaWI 696 TCCGGA [AT]^CCGG[AT]
BseAI 696 TCCGGA T^CCGGA
BsgI 643 GTGCAG GTGCAG(16/14)
BspEI 696 TCCGGA T^CCGGA
BssSI 851 CTCGTG CACGAG(-5/-1)
CelII 124 GCTGAGC GC^T[ACGT]AGC
DsaI 42 CCATGG C^C[GA][CT]GG
EaeI 45 TGGCCA [CT]^GGCC[GA]
Eam1105I 349 GACGAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 566 CCTGGCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HapII 697 CCGG C^CGG
HincII 912 GTTGAC GT[CT]^[GA]AC
HindII 912 GTTGAC GT[CT]^[GA]AC
HpaII 697 CCGG C^CGG
HphI 662 TCACC GGTGA(8/7)
MaeI 607 CTAG C^TAG
MfeI 585 CAATTG C^AATTG
MluNI 45 TGGCCA TGG^CCA
MroI 696 TCCGGA T^CCGGA
MscI 45 TGGCCA TGG^CCA
MspI 697 CCGG C^CGG
MunI 585 CAATTG C^AATTG
NcoI 42 CCATGG C^CATGG
PflFI 191 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 227 CCAATCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 50 AGGTCCC [GA]G^G[AT]CC[CT]
RsaI 101 GTAC GT^AC
SacI 457 GAGCTC GAGCT^C
SapI 708 GAAGAGC GCTCTTC(1/4)
SmlI 769 CTTAAG C^T[CT][GA]AG
TfiI 881 GAATC G^A[AT]TC
Tsp45I 449 GTGAC ^GT[GC]AC
Tth111I 191 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 227 CCAATCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AciI 831 CCGC CCGC(-3/-1)
834 CCGC
BanI 756 GGTGCC G^G[CT][GA]CC
827 GGCACC
BbvCI 331 CCTCAGC CCTCAGC(-5/-2)
475 GCTGAGG
BbvI 272 GCAGC GCAGC(8/12)
60 GCTGC
BpmI 402 CTCCAG CTGGAG(16/14)
499 CTCCAG
BstYI 306 GGATCT [GA]^GATC[CT]
650 AGATCT
EcoT14I 42 CCATGG C^C[AT][AT]GG
267 CCTTGG
MaeIII 449 GTGAC ^GT[ACGT]AC
521 GTTAC
MflI 306 GGATCT [GA]^GATC[CT]
650 AGATCT
MwoI 263 GCCCCCTTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
704 GCTGGAAGAGC
StyI 42 CCATGG C^C[AT][AT]GG
267 CCTTGG
TseI 60 GCTGC G^C[AT]GC
272 GCAGC
XhoII 306 GGATCT [GA]^GATC[CT]
650 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AluI AspHI AvaII BmyI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII Fnu4HI FokI
HaeIII HinfI ItaI Ksp632I MboI
MboII MnlI MseI MvaI NdeII
NlaIII NlaIV PleI Sau3AI Sau96I
ScrFI Tru9I Tsp509I TspRI