(Circular) (Six-base) MAPSORT of: pFN21AB9816.seq Check: 2176 from: 1 to: 5605 pFN21AB9816 5605 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3415 278 Size: 53 83 186 936 1879 2468 Fragments arranged by size: 2468 1879 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2133 2948 1937 Size: 196 815 4594 Fragments arranged by size: 4594 815 196 Acc65I G'GTAC_C Cuts at: 2926 2926 Size: 5605 AclI AA'CG_TT Cuts at: 1563 3732 4105 1563 Size: 2169 373 3063 Fragments arranged by size: 3063 2169 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2207 2539 2879 3668 5052 5320 1632 Size: 575 332 340 789 1384 268 1917 Fragments arranged by size: 1917 1384 789 575 340 332 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5408 AflIII A'CryG_T Cuts at: 1203 4504 5481 1203 Size: 3301 977 1327 Fragments arranged by size: 3301 1327 977 AgeI A'CCGG_T Cuts at: 2831 4436 2831 Size: 1605 4000 AhdI GACnn_n'nnGTC Cuts at: 4334 4334 Size: 5605 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5605 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5605 AlwNI CAG_nnn'CTG Cuts at: 3348 4920 3348 Size: 1572 4033 ApaI G_GGCC'C Cuts at: 2401 2692 2401 Size: 291 5314 ApaLI G'TGCA_C Cuts at: 3662 4818 3662 Size: 1156 4449 ApoI r'AATT_y Cuts at: 1493 2914 3140 3292 5401 1493 Size: 1421 226 152 2109 1697 Fragments arranged by size: 2109 1697 1421 226 152 AseI AT'TA_AT Cuts at: 160 1032 4159 160 Size: 872 3127 1606 Fragments arranged by size: 3127 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5605 AvaI C'yCGr_G Cuts at: 1870 1943 2930 1870 Size: 73 987 4545 Fragments arranged by size: 4545 987 73 BamHI G'GATC_C Cuts at: 2935 3302 2935 Size: 367 5238 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2798 2926 4381 618 Size: 325 217 627 1011 128 1455 1842 Fragments arranged by size: 1842 1455 1011 627 325 217 128 BanII G_rGCy'C Cuts at: 1391 2401 2692 2790 2924 1391 Size: 1010 291 98 134 4072 Fragments arranged by size: 4072 1010 291 134 98 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2239 2867 928 Size: 971 340 628 3666 Fragments arranged by size: 3666 971 628 340 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3796 3830 1903 Size: 34 1859 34 3678 Fragments arranged by size: 3678 1859 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3497 4707 3497 Size: 1210 4395 BclI T'GATC_A Cuts at: 1548 1548 Size: 5605 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2213 2396 2546 Size: 107 122 71 631 605 541 183 150 Cuts at: 2546 2678 2687 4216 136 Size: 132 9 1529 1525 Fragments arranged by size: 1529 1525 631 605 541 183 150 132 122 107 71 9 BglII A'GATC_T Cuts at: 5600 5600 Size: 5605 BlpI GC'TnA_GC Cuts at: 3022 3022 Size: 5605 Bme1580I G_kGCm'C Cuts at: 1790 2243 2401 2629 2692 3666 4822 1790 Size: 453 158 228 63 974 1156 2573 Fragments arranged by size: 2573 1156 974 453 228 158 63 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5605 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3336 4294 442 Size: 416 53 83 399 24 1919 958 1753 Fragments arranged by size: 1919 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2207 1056 Size: 1151 4454 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2427 2537 2824 4265 1239 Size: 1188 110 287 1441 2579 Fragments arranged by size: 2579 1441 1188 287 110 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3730 4595 4893 3730 Size: 865 298 4442 Fragments arranged by size: 4442 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4268 882 Size: 617 2769 2219 Fragments arranged by size: 2769 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2611 3396 493 Size: 711 1407 785 2702 Fragments arranged by size: 2702 1407 785 711 BsaBI GATnn'nnATC Cuts at: 5599 5599 Size: 5605 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3412 3794 4429 275 Size: 53 83 186 936 1879 382 635 1451 Fragments arranged by size: 1879 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2355 2506 2831 4038 4436 4710 4857 5268 2355 Size: 151 325 1207 398 274 147 411 2692 Fragments arranged by size: 2692 1207 411 398 325 274 151 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2714 2744 1178 Size: 30 165 30 1311 30 4039 Fragments arranged by size: 4039 1311 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2684 1186 Size: 1498 4107 BseYI C'CCAG_C Cuts at: 2056 2782 4808 5304 2056 Size: 726 2026 496 2357 Fragments arranged by size: 2357 2026 726 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2328 1163 Size: 573 592 4440 Fragments arranged by size: 4440 592 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3055 3816 4844 1540 Size: 275 186 1054 761 1028 2301 Fragments arranged by size: 2301 1054 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3666 3751 4822 3666 Size: 85 1071 4449 Fragments arranged by size: 4449 1071 85 BsmI GAATG_Cn' Cuts at: 3116 3209 3116 Size: 93 5512 BsmBI CGTCTCn'nnnn_ Cuts at: 2120 2120 Size: 5605 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2243 2401 2629 2692 2790 2924 3666 Size: 399 453 158 228 63 98 134 742 Cuts at: 3666 3751 4822 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 453 399 228 158 134 98 85 63 BspEI T'CCGG_A Cuts at: 2355 5268 2355 Size: 2913 2692 BspHI T'CATG_A Cuts at: 3494 5224 3494 Size: 1730 3875 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2019 2169 2960 844 Size: 1175 150 791 3489 Fragments arranged by size: 3489 1175 791 150 BsrBI CCG'CTC Cuts at: 1394 1823 2068 2637 3492 1394 Size: 429 245 569 855 3507 Fragments arranged by size: 3507 855 569 429 245 BsrDI GCAATG_nn' Cuts at: 65 1248 4100 4274 65 Size: 1183 2852 174 1396 Fragments arranged by size: 2852 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2227 2279 2491 2540 2681 2831 4249 Size: 110 417 52 212 49 141 150 1418 Cuts at: 4249 4436 1700 Size: 187 2869 Fragments arranged by size: 2869 1418 417 212 187 150 141 110 52 49 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5605 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5605 BssSI C'ACGA_G Cuts at: 3665 4677 3665 Size: 1012 4593 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5605 BstBI TT'CG_AA Cuts at: 4409 4409 Size: 5605 BstEII G'GTnAC_C Cuts at: 1184 2827 1184 Size: 1643 3962 BstXI CCAn_nnnn'nTGG Cuts at: 2451 2451 Size: 5605 BstYI r'GATC_y Cuts at: 1972 2935 3302 3687 3704 5156 5242 5254 5600 Size: 963 367 385 17 1452 86 12 346 Cuts at: 5600 1972 Size: 1977 Fragments arranged by size: 1977 1452 963 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4114 Fragments arranged by size: 4114 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4866 Fragments arranged by size: 4866 683 56 BtsI GCAGTG_nn' Cuts at: 3121 3915 3935 3121 Size: 794 20 4791 Fragments arranged by size: 4791 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5605 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5570 DraI TTT'AAA Cuts at: 2909 2909 Size: 5605 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4612 809 Size: 507 3296 1802 Fragments arranged by size: 3296 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2043 2079 2295 Size: 54 1425 50 275 18 213 36 216 Cuts at: 2295 2388 2589 2679 2739 3052 3941 5570 8 Size: 93 201 90 60 313 889 1629 43 Fragments arranged by size: 1629 1425 889 313 275 216 213 201 93 90 60 54 50 43 36 18 EagI C'GGCC_G Cuts at: 1537 1812 3052 1537 Size: 275 1240 4090 Fragments arranged by size: 4090 1240 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3535 1353 Size: 2182 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2671 2791 4192 4566 4712 387 Size: 1064 138 1082 120 1401 374 146 1280 Fragments arranged by size: 1401 1280 1082 1064 374 146 138 120 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2207 2427 2537 2539 2824 2879 3668 Size: 393 575 220 110 2 285 55 789 Cuts at: 3668 4265 5052 5320 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 789 787 597 575 393 285 268 220 110 55 2 EcoNI CCTnn'n_nnAGG Cuts at: 2356 2356 Size: 5605 EcoO109I rG'GnC_Cy Cuts at: 2214 2398 2527 2688 2689 2717 2849 2214 Size: 184 129 161 1 28 132 4970 Fragments arranged by size: 4970 184 161 132 129 28 1 EcoRI G'AATT_C Cuts at: 2914 3292 2914 Size: 378 5227 FspI TGC'GCA Cuts at: 4111 4111 Size: 5605 HaeII r_GCGC'y Cuts at: 1678 2204 4752 1678 Size: 526 2548 2531 Fragments arranged by size: 2548 2531 526 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2161 2193 2519 2551 4246 Size: 32 135 32 589 32 326 32 1695 Cuts at: 4246 4278 4320 4352 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1695 589 326 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2497 2949 3195 669 Size: 988 281 559 452 246 3079 Fragments arranged by size: 3079 988 559 452 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5605 HpaI GTT'AAC Cuts at: 3195 3195 Size: 5605 KpnI G_GTAC'C Cuts at: 2930 2930 Size: 5605 MfeI C'AATT_G Cuts at: 3204 3204 Size: 5605 MluI A'CGCG_T Cuts at: 5481 5481 Size: 5605 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2181 2415 2757 4719 4903 1305 Size: 876 234 342 1962 184 2007 Fragments arranged by size: 2007 1962 876 342 234 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2081 2591 2741 10 Size: 54 1425 343 249 510 150 2874 Fragments arranged by size: 2874 1425 510 343 249 150 54 MslI CAynn'nnrTG Cuts at: 518 1166 3563 3922 4081 518 Size: 648 2397 359 159 2042 Fragments arranged by size: 2397 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2824 3021 3698 4846 5091 1606 Size: 1218 197 677 1148 245 2120 Fragments arranged by size: 2120 1218 1148 677 245 197 NaeI GCC'GGC Cuts at: 2229 2281 2542 2683 2229 Size: 52 261 141 5151 Fragments arranged by size: 5151 261 141 52 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4114 Fragments arranged by size: 4114 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5605 NgoMIV G'CCGG_C Cuts at: 2227 2279 2540 2681 2227 Size: 52 261 141 5151 Fragments arranged by size: 5151 261 141 52 NheI G'CTAG_C Cuts at: 1052 2203 1052 Size: 1151 4454 NotI GC'GGCC_GC Cuts at: 3052 3052 Size: 5605 NspI r_CATG'y Cuts at: 1132 1229 2963 4508 1132 Size: 97 1734 1545 2229 Fragments arranged by size: 2229 1734 1545 97 PasI CC'CwG_GG Cuts at: 1348 2476 1348 Size: 1128 4477 PciI A'CATG_T Cuts at: 4504 4504 Size: 5605 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5605 PfoI T'CCnGG_A Cuts at: 2443 2443 Size: 5605 PmeI GTTT'AAAC Cuts at: 2909 2909 Size: 5605 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3674 3706 5187 5219 3674 Size: 32 1481 32 4060 Fragments arranged by size: 4060 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2527 2717 2849 2527 Size: 190 132 5283 Fragments arranged by size: 5283 190 132 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5605 PsiI TTA'TAA Cuts at: 3175 3175 Size: 5605 PspOMI G'GGCC_C Cuts at: 2397 2688 2397 Size: 291 5314 PstI C_TGCA'G Cuts at: 830 2957 830 Size: 2127 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5605 SalI G'TCGA_C Cuts at: 1936 2947 1936 Size: 1011 4594 SanDI GG'GwC_CC Cuts at: 2527 2527 Size: 5605 SbfI CC_TGCA'GG Cuts at: 2957 2957 Size: 5605 ScaI AGT'ACT Cuts at: 3853 5565 3853 Size: 1712 3893 SfcI C'TryA_G Cuts at: 826 1045 2953 4088 4769 4960 826 Size: 219 1908 1135 681 191 1471 Fragments arranged by size: 1908 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2396 2687 2396 Size: 291 5314 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5605 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5605 SmaI CCC'GGG Cuts at: 1872 2932 1872 Size: 1060 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3709 4610 4872 820 Size: 197 926 1766 901 262 1553 Fragments arranged by size: 1766 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3396 493 Size: 2903 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5605 SphI G_CATG'C Cuts at: 1132 2963 1132 Size: 1831 3774 SspI AAT'ATT Cuts at: 5 52 3529 5 Size: 47 3477 2081 Fragments arranged by size: 3477 2081 47 StuI AGG'CCT Cuts at: 1517 2522 1517 Size: 1005 4600 StyI C'CwwG_G Cuts at: 513 1065 2004 2337 2586 3044 513 Size: 552 939 333 249 458 3074 Fragments arranged by size: 3074 939 552 458 333 249 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2665 3632 3649 3802 3987 4432 325 Size: 1255 1085 967 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1085 967 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3851 5563 96 Size: 274 80 33 51 1443 1874 1712 138 Fragments arranged by size: 1874 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5565 5597 5565 Size: 32 5573 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5605 XbaI T'CTAG_A Cuts at: 2941 2941 Size: 5605 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5605 XmaI C'CCGG_G Cuts at: 1870 2930 1870 Size: 1060 4545 XmnI GAAnn'nnTTC Cuts at: 3734 3734 Size: 5605 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3413 276 Size: 53 83 186 936 1879 2468 Fragments arranged by size: 2468 1879 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfoI SrfI SwaI XcmI