(Circular) (Six-base) MAPSORT of: pFN21AB9811.seq Check: 1308 from: 1 to: 5449 pFN21AB9811 5449 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3259 278 Size: 53 83 186 936 1723 2468 Fragments arranged by size: 2468 1723 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2792 1937 Size: 855 4594 Acc65I G'GTAC_C Cuts at: 2770 2770 Size: 5449 AclI AA'CG_TT Cuts at: 1563 3576 3949 1563 Size: 2013 373 3063 Fragments arranged by size: 3063 2013 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2387 3512 4896 5164 1632 Size: 755 1125 1384 268 1917 Fragments arranged by size: 1917 1384 1125 755 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5252 AflIII A'CryG_T Cuts at: 1203 4348 5325 1203 Size: 3145 977 1327 Fragments arranged by size: 3145 1327 977 AgeI A'CCGG_T Cuts at: 2411 4280 2411 Size: 1869 3580 AhdI GACnn_n'nnGTC Cuts at: 4178 4178 Size: 5449 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5449 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5449 AlwNI CAG_nnn'CTG Cuts at: 2671 3192 4764 2671 Size: 521 1572 3356 Fragments arranged by size: 3356 1572 521 ApaI G_GGCC'C Cuts at: 2241 2241 Size: 5449 ApaLI G'TGCA_C Cuts at: 3506 4662 3506 Size: 1156 4293 ApoI r'AATT_y Cuts at: 1493 2758 2984 3136 5245 1493 Size: 1265 226 152 2109 1697 Fragments arranged by size: 2109 1697 1265 226 152 AseI AT'TA_AT Cuts at: 160 1032 4003 160 Size: 872 2971 1606 Fragments arranged by size: 2971 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5449 AvaI C'yCGr_G Cuts at: 1870 1943 2240 2564 2774 1870 Size: 73 297 324 210 4545 Fragments arranged by size: 4545 324 297 210 73 BamHI G'GATC_C Cuts at: 2779 3146 2779 Size: 367 5082 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2087 2770 4225 618 Size: 325 217 627 300 683 1455 1842 Fragments arranged by size: 1842 1455 683 627 325 300 217 BanII G_rGCy'C Cuts at: 1391 2112 2225 2241 2280 2742 2768 1391 Size: 721 113 16 39 462 26 4072 Fragments arranged by size: 4072 721 462 113 39 26 16 BbeI G_GCGC'C Cuts at: 2091 2091 Size: 5449 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2350 2530 928 Size: 971 451 180 3847 Fragments arranged by size: 3847 971 451 180 BbvCI CC'TCA_GC Cuts at: 2627 2627 Size: 5449 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3640 3674 1903 Size: 34 1703 34 3678 Fragments arranged by size: 3678 1703 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3341 4551 3341 Size: 1210 4239 BclI T'GATC_A Cuts at: 1548 1548 Size: 5449 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2039 2405 4060 Size: 107 122 71 631 605 367 366 1655 Cuts at: 4060 136 Size: 1525 Fragments arranged by size: 1655 1525 631 605 367 366 122 107 71 BglII A'GATC_T Cuts at: 5444 5444 Size: 5449 BlpI GC'TnA_GC Cuts at: 2866 2866 Size: 5449 Bme1580I G_kGCm'C Cuts at: 1790 2241 3510 4666 1790 Size: 451 1269 1156 2573 Fragments arranged by size: 2573 1269 1156 451 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5449 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3180 4138 442 Size: 416 53 83 399 24 1763 958 1753 Fragments arranged by size: 1763 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5449 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2128 2239 2593 2128 Size: 111 354 4984 Fragments arranged by size: 4984 354 111 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2200 2264 4109 1239 Size: 961 64 1845 2579 Fragments arranged by size: 2579 1845 961 64 Bpu10I CC'TnA_GC Cuts at: 2627 2627 Size: 5449 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3574 4439 4737 3574 Size: 865 298 4286 Fragments arranged by size: 4286 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4112 882 Size: 617 2613 2219 Fragments arranged by size: 2613 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3240 493 Size: 711 2036 2702 Fragments arranged by size: 2702 2036 711 BsaBI GATnn'nnATC Cuts at: 5443 5443 Size: 5449 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2088 2343 3256 3638 Size: 53 83 186 936 555 255 913 382 Cuts at: 3638 4273 275 Size: 635 1451 Fragments arranged by size: 1451 936 913 635 555 382 255 186 83 53 BsaWI w'CCGG_w Cuts at: 2411 3882 4280 4554 4701 5112 2411 Size: 1471 398 274 147 411 2748 Fragments arranged by size: 2748 1471 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5224 Fragments arranged by size: 5224 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2120 2231 2243 2582 1186 Size: 934 111 12 339 4053 Fragments arranged by size: 4053 934 339 111 12 BseYI C'CCAG_C Cuts at: 2302 4652 5148 2302 Size: 2350 496 2603 Fragments arranged by size: 2603 2350 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2603 1163 Size: 573 867 4009 Fragments arranged by size: 4009 867 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2700 2899 3660 4688 1540 Size: 275 186 699 199 761 1028 2301 Fragments arranged by size: 2301 1028 761 699 275 199 186 BsiHKAI G_wGCw'C Cuts at: 2112 2280 3510 3595 4666 2112 Size: 168 1230 85 1071 2895 Fragments arranged by size: 2895 1230 1071 168 85 BsmI GAATG_Cn' Cuts at: 2960 3053 2960 Size: 93 5356 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2112 2225 2241 2280 2742 2768 3510 Size: 399 322 113 16 39 462 26 742 Cuts at: 3510 3595 4666 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 462 399 322 113 85 39 26 16 BspEI T'CCGG_A Cuts at: 5112 5112 Size: 5449 BspHI T'CATG_A Cuts at: 2079 3338 5068 2079 Size: 1259 1730 2460 Fragments arranged by size: 2460 1730 1259 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2139 2804 844 Size: 1295 665 3489 Fragments arranged by size: 3489 1295 665 BsrBI CCG'CTC Cuts at: 1394 1823 3336 1394 Size: 429 1513 3507 Fragments arranged by size: 3507 1513 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3944 4118 65 Size: 1183 2696 174 1396 Fragments arranged by size: 2696 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2411 4093 4280 1700 Size: 110 601 1682 187 2869 Fragments arranged by size: 2869 1682 601 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5449 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5449 BssSI C'ACGA_G Cuts at: 3509 4521 3509 Size: 1012 4437 BstAPI GCAn_nnn'nTGC Cuts at: 794 2671 794 Size: 1877 3572 BstBI TT'CG_AA Cuts at: 4253 4253 Size: 5449 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5449 BstXI CCAn_nnnn'nTGG Cuts at: 2512 2581 2512 Size: 69 5380 BstYI r'GATC_y Cuts at: 1972 2779 3146 3531 3548 5000 5086 5098 5444 Size: 807 367 385 17 1452 86 12 346 Cuts at: 5444 1972 Size: 1977 Fragments arranged by size: 1977 1452 807 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2091 2466 2607 513 Size: 552 939 87 375 141 3355 Fragments arranged by size: 3355 939 552 375 141 87 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4710 Fragments arranged by size: 4710 683 56 BtsI GCAGTG_nn' Cuts at: 2965 3759 3779 2965 Size: 794 20 4635 Fragments arranged by size: 4635 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5449 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5414 DraI TTT'AAA Cuts at: 2753 2753 Size: 5449 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4456 809 Size: 507 3140 1802 Fragments arranged by size: 3140 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2164 2397 2406 Size: 54 1425 50 275 18 334 233 9 Cuts at: 2406 2583 2697 2896 3785 5414 8 Size: 177 114 199 889 1629 43 Fragments arranged by size: 1629 1425 889 334 275 233 199 177 114 54 50 43 18 9 EagI C'GGCC_G Cuts at: 1537 1812 2697 2896 1537 Size: 275 885 199 4090 Fragments arranged by size: 4090 885 275 199 EarI CTCTTCn'nnn_ Cuts at: 1353 2115 2709 3379 1353 Size: 762 594 670 3423 Fragments arranged by size: 3423 762 670 594 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2498 4036 4410 4556 387 Size: 1064 138 909 1538 374 146 1280 Fragments arranged by size: 1538 1280 1064 909 374 146 138 EcoICRI GAG'CTC Cuts at: 2110 2278 2110 Size: 168 5281 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2200 2264 2387 3512 4109 4896 5164 Size: 393 568 64 123 1125 597 787 268 Cuts at: 5164 1239 Size: 1524 Fragments arranged by size: 1524 1125 787 597 568 393 268 123 64 EcoNI CCTnn'n_nnAGG Cuts at: 2327 2399 2630 2327 Size: 72 231 5146 Fragments arranged by size: 5146 231 72 EcoO109I rG'GnC_Cy Cuts at: 2237 2237 Size: 5449 EcoRI G'AATT_C Cuts at: 2758 3136 2758 Size: 378 5071 FspI TGC'GCA Cuts at: 3955 3955 Size: 5449 HaeII r_GCGC'y Cuts at: 1678 2091 4596 1678 Size: 413 2505 2531 Fragments arranged by size: 2531 2505 413 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2002 2034 4090 4122 4164 Size: 32 135 32 430 32 2056 32 42 Cuts at: 4164 4196 1373 Size: 32 2626 Fragments arranged by size: 2626 2056 430 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2793 3039 669 Size: 988 281 855 246 3079 Fragments arranged by size: 3079 988 855 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5449 HpaI GTT'AAC Cuts at: 3039 3039 Size: 5449 KasI G'GCGC_C Cuts at: 2087 2087 Size: 5449 KpnI G_GTAC'C Cuts at: 2774 2774 Size: 5449 MfeI C'AATT_G Cuts at: 3048 3048 Size: 5449 MluI A'CGCG_T Cuts at: 5325 5325 Size: 5449 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4563 4747 1305 Size: 3258 184 2007 Fragments arranged by size: 3258 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2408 2585 10 Size: 54 1425 343 576 177 2874 Fragments arranged by size: 2874 1425 576 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 3407 3766 3925 518 Size: 648 2241 359 159 2042 Fragments arranged by size: 2241 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2668 2865 3542 4690 4935 1606 Size: 1062 197 677 1148 245 2120 Fragments arranged by size: 2120 1148 1062 677 245 197 NarI GG'CG_CC Cuts at: 2088 2088 Size: 5449 NcoI C'CATG_G Cuts at: 513 1065 2004 2466 2607 513 Size: 552 939 462 141 3355 Fragments arranged by size: 3355 939 552 462 141 NdeI CA'TA_TG Cuts at: 387 387 Size: 5449 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5449 NotI GC'GGCC_GC Cuts at: 2896 2896 Size: 5449 NspI r_CATG'y Cuts at: 1132 1229 2807 4352 1132 Size: 97 1578 1545 2229 Fragments arranged by size: 2229 1578 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5449 PciI A'CATG_T Cuts at: 4348 4348 Size: 5449 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5449 PmeI GTTT'AAAC Cuts at: 2753 2753 Size: 5449 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3518 3550 5031 5063 3518 Size: 32 1481 32 3904 Fragments arranged by size: 3904 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5449 PsiI TTA'TAA Cuts at: 3019 3019 Size: 5449 PspOMI G'GGCC_C Cuts at: 2237 2237 Size: 5449 PstI C_TGCA'G Cuts at: 830 2801 830 Size: 1971 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5449 PvuII CAG'CTG Cuts at: 2668 2668 Size: 5449 SacI G_AGCT'C Cuts at: 2112 2280 2112 Size: 168 5281 SalI G'TCGA_C Cuts at: 1936 2791 1936 Size: 855 4594 SapI GCTCTTCn'nnn_ Cuts at: 2115 2115 Size: 5449 SbfI CC_TGCA'GG Cuts at: 2801 2801 Size: 5449 ScaI AGT'ACT Cuts at: 3697 5409 3697 Size: 1712 3737 SfcI C'TryA_G Cuts at: 826 1045 2797 3932 4613 4804 826 Size: 219 1752 1135 681 191 1471 Fragments arranged by size: 1752 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2405 2405 Size: 5449 SfoI GGC'GCC Cuts at: 2089 2089 Size: 5449 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5449 SgrAI Cr'CCGG_yG Cuts at: 1700 2411 1700 Size: 711 4738 SmaI CCC'GGG Cuts at: 1872 2776 1872 Size: 904 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3553 4454 4716 820 Size: 197 926 1610 901 262 1553 Fragments arranged by size: 1610 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3240 493 Size: 2747 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5449 SphI G_CATG'C Cuts at: 1132 2807 1132 Size: 1675 3774 SspI AAT'ATT Cuts at: 5 52 3373 5 Size: 47 3321 2081 Fragments arranged by size: 3321 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5449 StyI C'CwwG_G Cuts at: 513 1065 2004 2466 2607 2888 513 Size: 552 939 462 141 281 3074 Fragments arranged by size: 3074 939 552 462 281 141 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3476 3493 3646 3831 4276 325 Size: 1255 1896 17 153 185 445 1498 Fragments arranged by size: 1896 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3695 5407 96 Size: 274 80 33 51 1443 1718 1712 138 Fragments arranged by size: 1718 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5409 5441 5409 Size: 32 5417 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5449 XbaI T'CTAG_A Cuts at: 2785 2785 Size: 5449 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5449 XmaI C'CCGG_G Cuts at: 1870 2774 1870 Size: 904 4545 XmnI GAAnn'nnTTC Cuts at: 3578 3578 Size: 5449 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3257 276 Size: 53 83 186 936 1723 2468 Fragments arranged by size: 2468 1723 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SrfI SwaI XcmI