(Circular) (Six-base) MAPSORT of: pFN21AB6661.seq Check: 8895 from: 1 to: 5977 pFN21AB6661 5977 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2252 3787 278 Size: 53 83 186 936 716 1535 2468 Fragments arranged by size: 2468 1535 936 716 186 83 53 AccI GT'mk_AC Cuts at: 1937 3320 1937 Size: 1383 4594 Acc65I G'GTAC_C Cuts at: 3298 3298 Size: 5977 AclI AA'CG_TT Cuts at: 1563 4104 4477 1563 Size: 2541 373 3063 Fragments arranged by size: 3063 2541 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3201 4040 5424 5692 1632 Size: 1569 839 1384 268 1917 Fragments arranged by size: 1917 1569 1384 839 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5780 AflIII A'CryG_T Cuts at: 1203 4876 5853 1203 Size: 3673 977 1327 Fragments arranged by size: 3673 1327 977 AgeI A'CCGG_T Cuts at: 4808 4808 Size: 5977 AhdI GACnn_n'nnGTC Cuts at: 4706 4706 Size: 5977 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5977 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2543 1184 Size: 1359 4618 AlwNI CAG_nnn'CTG Cuts at: 2209 2451 2717 3720 5292 2209 Size: 242 266 1003 1572 2894 Fragments arranged by size: 2894 1572 1003 266 242 ApaI G_GGCC'C Cuts at: 2234 2234 Size: 5977 ApaLI G'TGCA_C Cuts at: 4034 5190 4034 Size: 1156 4821 ApoI r'AATT_y Cuts at: 1493 2353 2391 3207 3286 3512 3664 5773 1493 Size: 860 38 816 79 226 152 2109 1697 Fragments arranged by size: 2109 1697 860 816 226 152 79 38 AseI AT'TA_AT Cuts at: 160 1032 4531 160 Size: 872 3499 1606 Fragments arranged by size: 3499 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5977 AvaI C'yCGr_G Cuts at: 1870 1943 2039 2234 3302 1870 Size: 73 96 195 1068 4545 Fragments arranged by size: 4545 1068 195 96 73 AvrII C'CTAG_G Cuts at: 2080 2080 Size: 5977 BamHI G'GATC_C Cuts at: 2430 3307 3674 2430 Size: 877 367 4733 Fragments arranged by size: 4733 877 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3298 4753 618 Size: 325 217 627 1511 1455 1842 Fragments arranged by size: 1842 1511 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2181 2234 3085 3296 1391 Size: 790 53 851 211 4072 Fragments arranged by size: 4072 851 790 211 53 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2749 928 Size: 971 850 4156 Fragments arranged by size: 4156 971 850 BbvCI CC'TCA_GC Cuts at: 3075 3075 Size: 5977 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4168 4202 1903 Size: 34 2231 34 3678 Fragments arranged by size: 3678 2231 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3869 5079 3869 Size: 1210 4767 BclI T'GATC_A Cuts at: 1548 2787 1548 Size: 1239 4738 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4588 136 Size: 107 122 71 631 605 2916 1525 Fragments arranged by size: 2916 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5972 5972 Size: 5977 BlpI GC'TnA_GC Cuts at: 2129 3394 2129 Size: 1265 4712 Bme1580I G_kGCm'C Cuts at: 1790 2234 4038 5194 1790 Size: 444 1804 1156 2573 Fragments arranged by size: 2573 1804 1156 444 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5977 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3708 4666 442 Size: 416 53 83 399 24 2291 958 1753 Fragments arranged by size: 2291 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5977 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4637 1239 Size: 3398 2579 Bpu10I CC'TnA_GC Cuts at: 3075 3167 3075 Size: 92 5885 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2894 3120 4102 4967 5265 2894 Size: 226 982 865 298 3606 Fragments arranged by size: 3606 982 865 298 226 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3264 4640 882 Size: 617 1765 1376 2219 Fragments arranged by size: 2219 1765 1376 617 BsaAI yAC'GTr Cuts at: 493 1204 3768 493 Size: 711 2564 2702 Fragments arranged by size: 2702 2564 711 BsaBI GATnn'nnATC Cuts at: 2429 2487 2792 5971 2429 Size: 58 305 3179 2435 Fragments arranged by size: 3179 2435 305 58 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2249 3784 4166 4801 Size: 53 83 186 936 716 1535 382 635 Cuts at: 4801 275 Size: 1451 Fragments arranged by size: 1535 1451 936 716 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2325 2499 4410 4808 5082 5229 5640 2325 Size: 174 1911 398 274 147 411 2662 Fragments arranged by size: 2662 1911 411 398 274 174 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3032 3062 3261 3291 1178 Size: 30 165 30 1629 30 199 30 3864 Fragments arranged by size: 3864 1629 199 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2785 1186 Size: 1599 4378 BseYI C'CCAG_C Cuts at: 2191 5180 5676 2191 Size: 2989 496 2492 Fragments arranged by size: 2989 2492 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5404 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2218 3427 4188 5216 1540 Size: 275 186 217 1209 761 1028 2301 Fragments arranged by size: 2301 1209 1028 761 275 217 186 BsiHKAI G_wGCw'C Cuts at: 2522 4038 4123 5194 2522 Size: 1516 85 1071 3305 Fragments arranged by size: 3305 1516 1071 85 BsmI GAATG_Cn' Cuts at: 3488 3581 3488 Size: 93 5884 BsmBI CGTCTCn'nnnn_ Cuts at: 2374 2374 Size: 5977 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2181 2234 2522 3085 3296 4038 4123 Size: 399 391 53 288 563 211 742 85 Cuts at: 4123 5194 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 563 399 391 288 211 85 53 BspEI T'CCGG_A Cuts at: 2499 5640 2499 Size: 3141 2836 BspHI T'CATG_A Cuts at: 3866 5596 3866 Size: 1730 4247 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2670 3332 844 Size: 1826 662 3489 Fragments arranged by size: 3489 1826 662 BsrBI CCG'CTC Cuts at: 1394 1823 2620 3864 1394 Size: 429 797 1244 3507 Fragments arranged by size: 3507 1244 797 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4472 4646 65 Size: 1183 3224 174 1396 Fragments arranged by size: 3224 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2019 2220 2257 4621 4808 1700 Size: 110 209 201 37 2364 187 2869 Fragments arranged by size: 2869 2364 209 201 187 110 37 BsrGI T'GTAC_A Cuts at: 96 2442 96 Size: 2346 3631 BssHII G'CGCG_C Cuts at: 1925 2278 1925 Size: 353 5624 BssSI C'ACGA_G Cuts at: 2123 2681 4037 5049 2123 Size: 558 1356 1012 3051 Fragments arranged by size: 3051 1356 1012 558 BstAPI GCAn_nnn'nTGC Cuts at: 794 2125 2209 2483 794 Size: 1331 84 274 4288 Fragments arranged by size: 4288 1331 274 84 BstBI TT'CG_AA Cuts at: 4781 4781 Size: 5977 BstEII G'GTnAC_C Cuts at: 1184 3245 1184 Size: 2061 3916 BstYI r'GATC_y Cuts at: 1972 2430 2793 3042 3307 3674 4059 4076 5528 Size: 458 363 249 265 367 385 17 1452 Cuts at: 5528 5614 5626 5972 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 458 385 367 363 346 265 249 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2064 2141 2226 3265 513 Size: 552 999 77 85 1039 3225 Fragments arranged by size: 3225 1039 999 552 85 77 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2438 487 Size: 683 56 1212 4026 Fragments arranged by size: 4026 1212 683 56 BtsI GCAGTG_nn' Cuts at: 2484 2727 3213 3493 4287 4307 2484 Size: 243 486 280 794 20 4154 Fragments arranged by size: 4154 794 486 280 243 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5977 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5942 DraI TTT'AAA Cuts at: 3281 3281 Size: 5977 DraIII CAC_nnn'GTG Cuts at: 2337 2337 Size: 5977 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4984 809 Size: 507 3668 1802 Fragments arranged by size: 3668 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2215 2556 3424 Size: 54 1425 50 275 18 385 341 868 Cuts at: 3424 4313 5942 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 868 385 341 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2215 3424 1537 Size: 275 403 1209 4090 Fragments arranged by size: 4090 1209 403 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3907 1353 Size: 2554 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4564 4938 5084 387 Size: 1064 138 2975 374 146 1280 Fragments arranged by size: 2975 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3201 4040 4637 5424 5692 1239 Size: 393 1569 839 597 787 268 1524 Fragments arranged by size: 1569 1524 839 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2027 2085 2231 2238 2551 2623 2027 Size: 58 146 7 313 72 5381 Fragments arranged by size: 5381 313 146 72 58 7 EcoRI G'AATT_C Cuts at: 3286 3664 3286 Size: 378 5599 EcoRV GAT'ATC Cuts at: 2890 2890 Size: 5977 FspI TGC'GCA Cuts at: 4483 4483 Size: 5977 HaeII r_GCGC'y Cuts at: 1678 2192 5124 1678 Size: 514 2932 2531 Fragments arranged by size: 2932 2531 514 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2354 2386 2772 2804 4618 Size: 32 135 32 782 32 386 32 1814 Cuts at: 4618 4650 4692 4724 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1814 782 386 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2362 3321 3567 669 Size: 988 281 424 959 246 3079 Fragments arranged by size: 3079 988 959 424 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5977 HpaI GTT'AAC Cuts at: 3567 3567 Size: 5977 KpnI G_GTAC'C Cuts at: 3302 3302 Size: 5977 MfeI C'AATT_G Cuts at: 3201 3576 3201 Size: 375 5602 MluI A'CGCG_T Cuts at: 5853 5853 Size: 5977 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5091 5275 1305 Size: 3786 184 2007 Fragments arranged by size: 3786 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2558 10 Size: 54 1425 343 726 3429 Fragments arranged by size: 3429 1425 726 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3935 4294 4453 518 Size: 648 2769 359 159 2042 Fragments arranged by size: 2769 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2143 2172 2206 2228 2288 2437 3393 4070 Size: 537 29 34 22 60 149 956 677 Cuts at: 4070 5218 5463 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 956 677 537 245 149 60 34 29 22 NaeI GCC'GGC Cuts at: 2021 2222 2259 2021 Size: 201 37 5739 Fragments arranged by size: 5739 201 37 NcoI C'CATG_G Cuts at: 513 1065 2064 3265 513 Size: 552 999 1201 3225 Fragments arranged by size: 3225 1201 999 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5977 NgoMIV G'CCGG_C Cuts at: 2019 2220 2257 2019 Size: 201 37 5739 Fragments arranged by size: 5739 201 37 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5977 NotI GC'GGCC_GC Cuts at: 3424 3424 Size: 5977 NspI r_CATG'y Cuts at: 1132 1229 3175 3335 4880 1132 Size: 97 1946 160 1545 2229 Fragments arranged by size: 2229 1946 1545 160 97 PasI CC'CwG_GG Cuts at: 1348 2968 1348 Size: 1620 4357 PciI A'CATG_T Cuts at: 4876 4876 Size: 5977 PflMI CCAn_nnn'nTGG Cuts at: 1108 2337 3266 1108 Size: 1229 929 3819 Fragments arranged by size: 3819 1229 929 PfoI T'CCnGG_A Cuts at: 3122 3122 Size: 5977 PmeI GTTT'AAAC Cuts at: 3281 3281 Size: 5977 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4046 4078 5559 5591 4046 Size: 32 1481 32 4432 Fragments arranged by size: 4432 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2027 2085 2238 2027 Size: 58 153 5766 Fragments arranged by size: 5766 153 58 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5977 PsiI TTA'TAA Cuts at: 3547 3547 Size: 5977 PspOMI G'GGCC_C Cuts at: 2230 2230 Size: 5977 PstI C_TGCA'G Cuts at: 830 2205 2722 2926 3329 830 Size: 1375 517 204 403 3478 Fragments arranged by size: 3478 1375 517 403 204 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5977 PvuII CAG'CTG Cuts at: 2206 2437 2206 Size: 231 5746 RsrII CG'GwC_CG Cuts at: 2266 2266 Size: 5977 SacII CC_GC'GG Cuts at: 2144 2229 2144 Size: 85 5892 SalI G'TCGA_C Cuts at: 1936 3319 1936 Size: 1383 4594 SanDI GG'GwC_CC Cuts at: 2238 2238 Size: 5977 SbfI CC_TGCA'GG Cuts at: 3329 3329 Size: 5977 ScaI AGT'ACT Cuts at: 4225 5937 4225 Size: 1712 4265 SfcI C'TryA_G Cuts at: 826 1045 2201 2718 2922 3325 4460 5141 5332 Size: 219 1156 517 204 403 1135 681 191 Cuts at: 5332 826 Size: 1471 Fragments arranged by size: 1471 1156 1135 681 517 403 219 204 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5977 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5977 SmaI CCC'GGG Cuts at: 1872 2236 3304 1872 Size: 364 1068 4545 Fragments arranged by size: 4545 1068 364 SmlI C'TyrA_G Cuts at: 820 1017 1943 2873 3135 4081 4982 5244 820 Size: 197 926 930 262 946 901 262 1553 Fragments arranged by size: 1553 946 930 926 901 262 262 197 SnaBI TAC'GTA Cuts at: 493 3768 493 Size: 3275 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5977 SphI G_CATG'C Cuts at: 1132 3175 3335 1132 Size: 2043 160 3774 Fragments arranged by size: 3774 2043 160 SspI AAT'ATT Cuts at: 5 52 3901 5 Size: 47 3849 2081 Fragments arranged by size: 3849 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5977 StyI C'CwwG_G Cuts at: 513 1065 2064 2080 3265 3416 513 Size: 552 999 16 1185 151 3074 Fragments arranged by size: 3074 1185 999 552 151 16 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2254 3102 3131 4004 4021 4174 4359 Size: 1255 674 848 29 873 17 153 185 Cuts at: 4359 4804 325 Size: 445 1498 Fragments arranged by size: 1498 1255 873 848 674 445 185 153 29 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2442 4223 5935 Size: 274 80 33 51 1443 465 1781 1712 Cuts at: 5935 96 Size: 138 Fragments arranged by size: 1781 1712 1443 465 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3032 3064 5937 5969 3032 Size: 32 2873 32 3040 Fragments arranged by size: 3040 2873 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2366 1316 Size: 1050 4927 XbaI T'CTAG_A Cuts at: 3313 3313 Size: 5977 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5977 XmaI C'CCGG_G Cuts at: 1870 2234 3302 1870 Size: 364 1068 4545 Fragments arranged by size: 4545 1068 364 XmnI GAAnn'nnTTC Cuts at: 4106 4106 Size: 5977 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2250 3785 276 Size: 53 83 186 936 716 1535 2468 Fragments arranged by size: 2468 1535 936 716 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI SacI SapI SexAI SfiI SfoI SrfI SwaI XcmI