(Linear) (Six-base) MAPSORT of: KIBB6661.seq Check: 8547 from: 1 to: 1272 KIBB6661.orf With 182 enzymes: SgfI * December 27, 2011 16:29 .. AatII G_ACGT'C Cuts at: 0 247 1272 Size: 247 1025 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1196 1272 Size: 1196 76 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 538 1272 Size: 538 734 AlwNI CAG_nnn'CTG Cuts at: 0 204 446 712 1272 Size: 204 242 266 560 Fragments arranged by size: 560 266 242 204 ApaI G_GGCC'C Cuts at: 0 229 1272 Size: 229 1043 ApoI r'AATT_y Cuts at: 0 348 386 1202 1272 Size: 348 38 816 70 Fragments arranged by size: 816 348 70 38 AvaI C'yCGr_G Cuts at: 0 34 229 1272 Size: 34 195 1043 Fragments arranged by size: 1043 195 34 AvrII C'CTAG_G Cuts at: 0 75 1272 Size: 75 1197 BamHI G'GATC_C Cuts at: 0 425 1272 Size: 425 847 BanII G_rGCy'C Cuts at: 0 176 229 1080 1272 Size: 176 53 851 192 Fragments arranged by size: 851 192 176 53 BbsI GAAGACnn'nnnn_ Cuts at: 0 744 1272 Size: 744 528 BbvCI CC'TCA_GC Cuts at: 0 1070 1272 Size: 1070 202 BclI T'GATC_A Cuts at: 0 782 1272 Size: 782 490 BlpI GC'TnA_GC Cuts at: 0 124 1272 Size: 124 1148 Bme1580I G_kGCm'C Cuts at: 0 229 1272 Size: 229 1043 Bpu10I CC'TnA_GC Cuts at: 0 1070 1162 1272 Size: 1070 92 110 Fragments arranged by size: 1070 110 92 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 889 1115 1272 Size: 889 226 157 Fragments arranged by size: 889 226 157 BsaI GGTCTCn'nnnn_ Cuts at: 0 1259 1272 Size: 1259 13 BsaBI GATnn'nnATC Cuts at: 0 424 482 787 1272 Size: 424 58 305 485 Fragments arranged by size: 485 424 305 58 BsaHI Gr'CG_yC Cuts at: 0 244 1272 Size: 244 1028 BsaWI w'CCGG_w Cuts at: 0 320 494 1272 Size: 320 174 778 Fragments arranged by size: 778 320 174 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1027 1057 1272 Size: 1027 30 215 Fragments arranged by size: 1027 215 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 780 1272 Size: 780 492 BseYI C'CCAG_C Cuts at: 0 186 1272 Size: 186 1086 BsiEI CG_ry'CG Cuts at: 0 213 1272 Size: 213 1059 BsiHKAI G_wGCw'C Cuts at: 0 517 1272 Size: 517 755 BsmBI CGTCTCn'nnnn_ Cuts at: 0 369 1272 Size: 369 903 Bsp1286I G_dGCh'C Cuts at: 0 176 229 517 1080 1272 Size: 176 53 288 563 192 Fragments arranged by size: 563 288 192 176 53 BspEI T'CCGG_A Cuts at: 0 494 1272 Size: 494 778 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 665 1272 Size: 665 607 BsrBI CCG'CTC Cuts at: 0 615 1272 Size: 615 657 BsrFI r'CCGG_y Cuts at: 0 14 215 252 1272 Size: 14 201 37 1020 Fragments arranged by size: 1020 201 37 14 BsrGI T'GTAC_A Cuts at: 0 437 1272 Size: 437 835 BssHII G'CGCG_C Cuts at: 0 273 1272 Size: 273 999 BssSI C'ACGA_G Cuts at: 0 118 676 1272 Size: 118 558 596 Fragments arranged by size: 596 558 118 BstAPI GCAn_nnn'nTGC Cuts at: 0 120 204 478 1272 Size: 120 84 274 794 Fragments arranged by size: 794 274 120 84 BstEII G'GTnAC_C Cuts at: 0 1240 1272 Size: 1240 32 BstYI r'GATC_y Cuts at: 0 425 788 1037 1272 Size: 425 363 249 235 Fragments arranged by size: 425 363 249 235 BtgI C'CryG_G Cuts at: 0 59 136 221 1260 1272 Size: 59 77 85 1039 12 Fragments arranged by size: 1039 85 77 59 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 433 1272 Size: 433 839 BtsI GCAGTG_nn' Cuts at: 0 479 722 1208 1272 Size: 479 243 486 64 Fragments arranged by size: 486 479 243 64 DraIII CAC_nnn'GTG Cuts at: 0 332 1272 Size: 332 940 EaeI y'GGCC_r Cuts at: 0 210 551 1272 Size: 210 341 721 Fragments arranged by size: 721 341 210 EagI C'GGCC_G Cuts at: 0 210 1272 Size: 210 1062 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1196 1272 Size: 1196 76 EcoO109I rG'GnC_Cy Cuts at: 0 22 80 226 233 546 618 1272 Size: 22 58 146 7 313 72 654 Fragments arranged by size: 654 313 146 72 58 22 7 EcoRV GAT'ATC Cuts at: 0 885 1272 Size: 885 387 HaeII r_GCGC'y Cuts at: 0 187 1272 Size: 187 1085 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 349 381 767 799 1272 Size: 349 32 386 32 473 Fragments arranged by size: 473 386 349 32 32 HincII GTy'rAC Cuts at: 0 357 1272 Size: 357 915 MfeI C'AATT_G Cuts at: 0 1196 1272 Size: 1196 76 MscI TGG'CCA Cuts at: 0 553 1272 Size: 553 719 MspA1I CmG'CkG Cuts at: 0 138 167 201 223 283 432 1272 Size: 138 29 34 22 60 149 840 Fragments arranged by size: 840 149 138 60 34 29 22 NaeI GCC'GGC Cuts at: 0 16 217 254 1272 Size: 16 201 37 1018 Fragments arranged by size: 1018 201 37 16 NcoI C'CATG_G Cuts at: 0 59 1260 1272 Size: 59 1201 12 Fragments arranged by size: 1201 59 12 NgoMIV G'CCGG_C Cuts at: 0 14 215 252 1272 Size: 14 201 37 1020 Fragments arranged by size: 1020 201 37 14 NspI r_CATG'y Cuts at: 0 1170 1272 Size: 1170 102 PasI CC'CwG_GG Cuts at: 0 963 1272 Size: 963 309 PflMI CCAn_nnn'nTGG Cuts at: 0 332 1261 1272 Size: 332 929 11 Fragments arranged by size: 929 332 11 PfoI T'CCnGG_A Cuts at: 0 1117 1272 Size: 1117 155 PpuMI rG'GwC_Cy Cuts at: 0 22 80 233 1272 Size: 22 58 153 1039 Fragments arranged by size: 1039 153 58 22 PspOMI G'GGCC_C Cuts at: 0 225 1272 Size: 225 1047 PstI C_TGCA'G Cuts at: 0 200 717 921 1272 Size: 200 517 204 351 Fragments arranged by size: 517 351 204 200 PvuII CAG'CTG Cuts at: 0 201 432 1272 Size: 201 231 840 Fragments arranged by size: 840 231 201 RsrII CG'GwC_CG Cuts at: 0 261 1272 Size: 261 1011 SacII CC_GC'GG Cuts at: 0 139 224 1272 Size: 139 85 1048 Fragments arranged by size: 1048 139 85 SanDI GG'GwC_CC Cuts at: 0 233 1272 Size: 233 1039 SfcI C'TryA_G Cuts at: 0 196 713 917 1272 Size: 196 517 204 355 Fragments arranged by size: 517 355 204 196 SmaI CCC'GGG Cuts at: 0 231 1272 Size: 231 1041 SmlI C'TyrA_G Cuts at: 0 868 1130 1272 Size: 868 262 142 Fragments arranged by size: 868 262 142 SphI G_CATG'C Cuts at: 0 1170 1272 Size: 1170 102 StyI C'CwwG_G Cuts at: 0 59 75 1260 1272 Size: 59 16 1185 12 Fragments arranged by size: 1185 59 16 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 249 1097 1126 1272 Size: 249 848 29 146 Fragments arranged by size: 848 249 146 29 TatI w'GTAC_w Cuts at: 0 437 1272 Size: 437 835 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1027 1059 1272 Size: 1027 32 213 Fragments arranged by size: 1027 213 32 Tth111I GACn'n_nGTC Cuts at: 0 361 1272 Size: 361 911 XmaI C'CCGG_G Cuts at: 0 229 1272 Size: 229 1043 ZraI GAC'GTC Cuts at: 0 245 1272 Size: 245 1027 Enzymes that do cut: AatII AcuI AlfI AlwNI ApaI ApoI AvaI AvrII BamHI BanII BbsI BbvCI BclI BlpI Bme1580I Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstYI BtgI BtgZI BtsI DraIII EaeI EagI Eco57MI EcoO109I EcoRV HaeII Hin4I HincII MfeI MscI MspA1I NaeI NcoI NgoMIV NspI PasI PflMI PfoI PpuMI PspOMI PstI PvuII RsrII SacII SanDI SfcI SmaI SmlI SphI StyI TaqII TatI TstI Tth111I XmaI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AloI ApaLI AscI AseI AsiSI BaeI BanI BbeI BcgI BciVI BfrBI BglI BglII BmgBI BmrI BmtI BplI BpmI BsaAI BsgI BsiWI BsmI BspHI BsrDI BstBI BstXI BstZ17I Bsu36I ClaI CspCI DraI DrdI EarI EciI EcoICRI EcoNI EcoRI FalI FseI FspI FspAI HindIII HpaI KasI KpnI MluI MmeI MslI NarI NdeI NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI SacI SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI XbaI XcmI XhoI XmnI