Restriction map for
pFN21AB9785
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# NO CUT ENZYMES ( 186 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BlnI BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmFI BsoBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I Cac8I CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HpaII
HphI KasI KpnI Ksp632I KspI
MaeII MaeIII MamI MfeI MluI
MluNI MroI MscI MslI MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI Tsp45I TspRI Tth111I
TthHB8I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AciI 409 GCGG CCGC(-3/-1)
AcsI 319 AAATTC [GA]^AATT[CT]
AlwNI 340 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 319 AAATTC [GA]^AATT[CT]
Asp700I 432 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 37 GTGCTC G[AT]GC[AT]^C
BfaI 313 CTAG C^TAG
BglII 257 AGATCT A^GATCT
BlpI 39 GCTCAGC GC^T[ACGT]AGC
BmyI 37 GTGCTC G[AGT]GC[ACT]^C
BpmI 221 CTCCAG CTGGAG(16/14)
Bpu1102I 39 GCTCAGC GC^T[ACGT]AGC
BsaI 82 GAGACC GGTCTC(1/5)
BsgI 324 CTGCAC GTGCAG(16/14)
BsiHKAI 37 GTGCTC G[AT]GC[AT]^C
BsiYI 87 CCACAGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 87 CCACAGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 82 GAGAC GTCTC(1/5)
BsmI 405 GAATGC GAATGC(1/-1)
Bsp1286I 37 GTGCTC G[AGT]GC[ACT]^C
BspHI 512 TCATGA T^CATGA
BsrI 207 CCAGT ACTGG(1/-1)
BstF5I 300 GGATG GGATG(2/0)
CelII 39 GCTCAGC GC^T[ACGT]AGC
FokI 300 GGATG GGATG(9/13)
HaeIII 3 GGCC GG^CC
MaeI 313 CTAG C^TAG
MseI 553 TTAA T^TAA
NlaIV 219 GGCTCC GG[ACGT]^[ACGT]CC
PflMI 87 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 412 GACTC GAGTC(4/5)
RcaI 512 TCATGA T^CATGA
Tru9I 553 TTAA T^TAA
Tsp509I 320 AATT ^AATT
Van91I 87 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 432 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AlwI 249 GATCC GGATC(4/5)
518 GATCC
BsaJI 185 CCCAGG C^C[ACGT][ACGT]GG
488 CCTGGG
BseRI 190 GAGGAG GAGGAG(10/8)
376 GAGGAG
BstYI 257 AGATCT [GA]^GATC[CT]
517 AGATCC
DdeI 40 CTCAG C^T[ACGT]AG
80 CTGAG
DraII 106 AGGACCT [GA]G^G[ACGT]CC[CT]
305 AGGACCT
EcoO109I 106 AGGACCT [GA]G^G[ACGT]CC[CT]
305 AGGACCT
MflI 257 AGATCT [GA]^GATC[CT]
517 AGATCC
MspA1I 42 CAGCTG C[AC]G^C[GT]G
343 CAGCTG
PpuMI 106 AGGACCT [GA]G^G[AT]CC[CT]
305 AGGACCT
PvuII 42 CAGCTG CAG^CTG
343 CAGCTG
SfaNI 114 GATGC GCATC(5/9)
369 GATGC
XhoII 257 AGATCT [GA]^GATC[CT]
517 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AluI AvaII BbvI BssKI BstNI
Cfr13I DpnII EcoRII Fnu4HI HinfI
ItaI MboI MboII MnlI MvaI
MwoI NdeII NlaIII Sau3AI Sau96I
ScrFI TfiI TseI