(Circular) (Six-base) MAPSORT of: pFN21AB9782.seq Check: 9905 from: 1 to: 5332 pFN21AB9782 5332 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3142 278 Size: 53 83 186 936 1606 2468 Fragments arranged by size: 2468 1606 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2675 1937 Size: 738 4594 Acc65I G'GTAC_C Cuts at: 2653 2653 Size: 5332 AclI AA'CG_TT Cuts at: 1563 3459 3832 1563 Size: 1896 373 3063 Fragments arranged by size: 3063 1896 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2309 2402 3395 4779 5047 1632 Size: 677 93 993 1384 268 1917 Fragments arranged by size: 1917 1384 993 677 268 93 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5135 AflIII A'CryG_T Cuts at: 1203 4231 5208 1203 Size: 3028 977 1327 Fragments arranged by size: 3028 1327 977 AgeI A'CCGG_T Cuts at: 4163 4163 Size: 5332 AhdI GACnn_n'nnGTC Cuts at: 4061 4061 Size: 5332 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5332 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5332 AlwNI CAG_nnn'CTG Cuts at: 3075 4647 3075 Size: 1572 3760 ApaI G_GGCC'C Cuts at: 2255 2598 2255 Size: 343 4989 ApaLI G'TGCA_C Cuts at: 2303 3389 4545 2303 Size: 1086 1156 3090 Fragments arranged by size: 3090 1156 1086 ApoI r'AATT_y Cuts at: 1493 2641 2867 3019 5128 1493 Size: 1148 226 152 2109 1697 Fragments arranged by size: 2109 1697 1148 226 152 AseI AT'TA_AT Cuts at: 160 1032 3886 160 Size: 872 2854 1606 Fragments arranged by size: 2854 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5332 AvaI C'yCGr_G Cuts at: 1870 1943 2657 1870 Size: 73 714 4545 Fragments arranged by size: 4545 714 73 BamHI G'GATC_C Cuts at: 2662 3029 2662 Size: 367 4965 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2242 2388 2570 2653 4108 Size: 325 217 627 455 146 182 83 1455 Cuts at: 4108 618 Size: 1842 Fragments arranged by size: 1842 1455 627 455 325 217 182 146 83 BanII G_rGCy'C Cuts at: 1391 2255 2447 2598 2651 1391 Size: 864 192 151 53 4072 Fragments arranged by size: 4072 864 192 151 53 BbeI G_GCGC'C Cuts at: 2392 2392 Size: 5332 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2163 928 Size: 971 264 4097 Fragments arranged by size: 4097 971 264 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2354 2388 3523 3557 1903 Size: 34 417 34 1135 34 3678 Fragments arranged by size: 3678 1135 417 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3224 4434 3224 Size: 1210 4122 BclI T'GATC_A Cuts at: 1548 1548 Size: 5332 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3943 136 Size: 107 122 71 631 605 2271 1525 Fragments arranged by size: 2271 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5327 5327 Size: 5332 BlpI GC'TnA_GC Cuts at: 2279 2749 2279 Size: 470 4862 Bme1580I G_kGCm'C Cuts at: 1790 2247 2255 2307 2341 2598 3393 4549 1790 Size: 457 8 52 34 257 795 1156 2573 Fragments arranged by size: 2573 1156 795 457 257 52 34 8 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5332 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3063 4021 442 Size: 416 53 83 399 24 1646 958 1753 Fragments arranged by size: 1753 1646 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5332 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2032 2225 2032 Size: 193 5139 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2054 2072 2339 2414 3992 1239 Size: 815 18 267 75 1578 2579 Fragments arranged by size: 2579 1578 815 267 75 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2216 2243 3457 4322 4620 2216 Size: 27 1214 865 298 2928 Fragments arranged by size: 2928 1214 865 298 27 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3995 882 Size: 617 2496 2219 Fragments arranged by size: 2496 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3123 493 Size: 711 1919 2702 Fragments arranged by size: 2702 1919 711 BsaBI GATnn'nnATC Cuts at: 5326 5326 Size: 5332 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2389 3139 3521 4156 Size: 53 83 186 936 856 750 382 635 Cuts at: 4156 275 Size: 1451 Fragments arranged by size: 1451 936 856 750 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3765 4163 4437 4584 4995 3765 Size: 398 274 147 411 4102 Fragments arranged by size: 4102 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2387 2417 1178 Size: 30 165 30 984 30 4093 Fragments arranged by size: 4093 984 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2336 1186 Size: 1150 4182 BseYI C'CCAG_C Cuts at: 2611 4535 5031 2611 Size: 1924 496 2912 Fragments arranged by size: 2912 1924 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2429 1163 Size: 573 693 4066 Fragments arranged by size: 4066 693 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2043 2139 2782 3543 4571 1540 Size: 275 186 42 96 643 761 1028 2301 Fragments arranged by size: 2301 1028 761 643 275 186 96 42 BsiHKAI G_wGCw'C Cuts at: 2079 2307 3393 3478 4549 2079 Size: 228 1086 85 1071 2862 Fragments arranged by size: 2862 1086 1071 228 85 BsmI GAATG_Cn' Cuts at: 2843 2936 2843 Size: 93 5239 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2079 2247 2255 2307 2341 2447 2598 Size: 399 289 168 8 52 34 106 151 Cuts at: 2598 2651 3393 3478 4549 1391 Size: 53 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 289 168 151 106 85 53 52 34 8 BspEI T'CCGG_A Cuts at: 4995 4995 Size: 5332 BspHI T'CATG_A Cuts at: 3221 4951 3221 Size: 1730 3602 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2687 844 Size: 1843 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3219 1394 Size: 429 1396 3507 Fragments arranged by size: 3507 1396 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3827 4001 65 Size: 1183 2579 174 1396 Fragments arranged by size: 2579 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2138 2573 2604 3976 4163 1700 Size: 110 328 435 31 1372 187 2869 Fragments arranged by size: 2869 1372 435 328 187 110 31 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5332 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5332 BssSI C'ACGA_G Cuts at: 3392 4404 3392 Size: 1012 4320 BstAPI GCAn_nnn'nTGC Cuts at: 794 2242 794 Size: 1448 3884 BstBI TT'CG_AA Cuts at: 4136 4136 Size: 5332 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5332 BstYI r'GATC_y Cuts at: 1972 2662 3029 3414 3431 4883 4969 4981 5327 Size: 690 367 385 17 1452 86 12 346 Cuts at: 5327 1972 Size: 1977 Fragments arranged by size: 1977 1452 690 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2457 513 Size: 552 939 453 3388 Fragments arranged by size: 3388 939 552 453 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4593 Fragments arranged by size: 4593 683 56 BtsI GCAGTG_nn' Cuts at: 2542 2599 2848 3642 3662 2542 Size: 57 249 794 20 4212 Fragments arranged by size: 4212 794 249 57 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5332 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5297 DraI TTT'AAA Cuts at: 2636 2636 Size: 5332 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4339 809 Size: 507 3023 1802 Fragments arranged by size: 3023 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2040 2136 2779 Size: 54 1425 50 275 18 210 96 643 Cuts at: 2779 3668 5297 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 643 275 210 96 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2040 2136 2779 1537 Size: 275 228 96 643 4090 Fragments arranged by size: 4090 643 275 228 96 EarI CTCTTCn'nnn_ Cuts at: 1353 3262 1353 Size: 1909 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3919 4293 4439 387 Size: 1064 138 2330 374 146 1280 Fragments arranged by size: 2330 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2054 2072 2309 2339 2402 2414 3395 Size: 393 422 18 237 30 63 12 981 Cuts at: 3395 3992 4779 5047 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 981 787 597 422 393 268 237 63 30 18 12 EcoNI CCTnn'n_nnAGG Cuts at: 2033 2033 Size: 5332 EcoO109I rG'GnC_Cy Cuts at: 2155 2252 2595 2155 Size: 97 343 4892 Fragments arranged by size: 4892 343 97 EcoRI G'AATT_C Cuts at: 2641 3019 2641 Size: 378 4954 FseI GG_CCGG'CC Cuts at: 2142 2142 Size: 5332 FspI TGC'GCA Cuts at: 2150 2429 3838 2150 Size: 279 1409 3644 Fragments arranged by size: 3644 1409 279 FspAI rTGC'GCAy Cuts at: 2429 2429 Size: 5332 HaeII r_GCGC'y Cuts at: 1678 2392 4479 1678 Size: 714 2087 2531 Fragments arranged by size: 2531 2087 714 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2387 2419 3973 4005 4047 Size: 32 135 32 815 32 1554 32 42 Cuts at: 4047 4079 1373 Size: 32 2626 Fragments arranged by size: 2626 1554 815 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2676 2922 669 Size: 988 281 738 246 3079 Fragments arranged by size: 3079 988 738 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5332 HpaI GTT'AAC Cuts at: 2922 2922 Size: 5332 KasI G'GCGC_C Cuts at: 2388 2388 Size: 5332 KpnI G_GTAC'C Cuts at: 2657 2657 Size: 5332 MfeI C'AATT_G Cuts at: 2931 2931 Size: 5332 MluI A'CGCG_T Cuts at: 5208 5208 Size: 5332 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4446 4630 1305 Size: 3141 184 2007 Fragments arranged by size: 3141 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3510 Fragments arranged by size: 3510 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3290 3649 3808 518 Size: 648 2124 359 159 2042 Fragments arranged by size: 2124 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2359 2401 2748 3425 4573 4818 1606 Size: 753 42 347 677 1148 245 2120 Fragments arranged by size: 2120 1148 753 677 347 245 42 NaeI GCC'GGC Cuts at: 2140 2606 2140 Size: 466 4866 NarI GG'CG_CC Cuts at: 2389 2389 Size: 5332 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3841 Fragments arranged by size: 3841 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5332 NgoMIV G'CCGG_C Cuts at: 2138 2604 2138 Size: 466 4866 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5332 NotI GC'GGCC_GC Cuts at: 2779 2779 Size: 5332 NspI r_CATG'y Cuts at: 1132 1229 2690 4235 1132 Size: 97 1461 1545 2229 Fragments arranged by size: 2229 1545 1461 97 PasI CC'CwG_GG Cuts at: 1348 2248 1348 Size: 900 4432 PciI A'CATG_T Cuts at: 4231 4231 Size: 5332 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5332 PmeI GTTT'AAAC Cuts at: 2636 2636 Size: 5332 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3401 3433 4914 4946 3401 Size: 32 1481 32 3787 Fragments arranged by size: 3787 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5332 PsiI TTA'TAA Cuts at: 2902 2902 Size: 5332 PspOMI G'GGCC_C Cuts at: 2251 2594 2251 Size: 343 4989 PstI C_TGCA'G Cuts at: 830 2364 2565 2684 830 Size: 1534 201 119 3478 Fragments arranged by size: 3478 1534 201 119 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5332 PvuII CAG'CTG Cuts at: 2359 2401 2359 Size: 42 5290 SalI G'TCGA_C Cuts at: 1936 2674 1936 Size: 738 4594 SbfI CC_TGCA'GG Cuts at: 2684 2684 Size: 5332 ScaI AGT'ACT Cuts at: 3580 5292 3580 Size: 1712 3620 SfcI C'TryA_G Cuts at: 826 1045 2360 2554 2561 2680 3815 4496 4687 Size: 219 1315 194 7 119 1135 681 191 Cuts at: 4687 826 Size: 1471 Fragments arranged by size: 1471 1315 1135 681 219 194 191 119 7 SfoI GGC'GCC Cuts at: 2390 2390 Size: 5332 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5332 SgrAI Cr'CCGG_yG Cuts at: 1700 2573 1700 Size: 873 4459 SmaI CCC'GGG Cuts at: 1872 2659 1872 Size: 787 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2222 2231 3436 4337 4599 820 Size: 197 926 279 9 1205 901 262 1553 Fragments arranged by size: 1553 1205 926 901 279 262 197 9 SnaBI TAC'GTA Cuts at: 493 3123 493 Size: 2630 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5332 SphI G_CATG'C Cuts at: 1132 2690 1132 Size: 1558 3774 SspI AAT'ATT Cuts at: 5 52 3256 5 Size: 47 3204 2081 Fragments arranged by size: 3204 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5332 StyI C'CwwG_G Cuts at: 513 1065 2004 2298 2771 513 Size: 552 939 294 473 3074 Fragments arranged by size: 3074 939 552 473 294 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3359 3376 3529 3714 4159 325 Size: 1255 1779 17 153 185 445 1498 Fragments arranged by size: 1779 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3578 5290 96 Size: 274 80 33 51 1443 1601 1712 138 Fragments arranged by size: 1712 1601 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5292 5324 5292 Size: 32 5300 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5332 XbaI T'CTAG_A Cuts at: 2668 2668 Size: 5332 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5332 XmaI C'CCGG_G Cuts at: 1870 2657 1870 Size: 787 4545 XmnI GAAnn'nnTTC Cuts at: 3461 3461 Size: 5332 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3140 276 Size: 53 83 186 936 1606 2468 Fragments arranged by size: 2468 1606 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI