Restriction map for
pFN21AB9779
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# NO CUT ENZYMES ( 140 enzymes) #
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AatII Acc65I AccI AclI AcyI
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BanII
BbeI BbrPI BcgI BciVI BfaI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsiEI BsiHKAI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraII DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRI FauI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HpaI
KasI KpnI KspI MaeI MamI
MluI MluNI MscI MspA1I NaeI
NarI NcoI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SwaI Tth111I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccII 1681 CGCG CG^CG
AccIII 1815 TCCGGA T^CCGGA
AciI 2538 CCGC CCGC(-3/-1)
AflII 61 CTTAAG C^TTAAG
BamHI 2304 GGATCC G^GATCC
BanI 2231 GGCACC G^G[CT][GA]CC
BbsI 118 GAAGAC GAAGAC(2/6)
BbvCI 2155 GCTGAGG CCTCAGC(-5/-2)
BclI 2201 TGATCA T^GATCA
BfrI 61 CTTAAG C^TTAAG
BpmI 2652 CTGGAG CTGGAG(16/14)
BpuAI 118 GAAGAC GAAGAC(2/6)
BsaWI 1815 TCCGGA [AT]^CCGG[AT]
BseAI 1815 TCCGGA T^CCGGA
BseRI 1367 CTCCTC GAGGAG(10/8)
BsmFI 344 GTCCC GGGAC(10/14)
BspEI 1815 TCCGGA T^CCGGA
BsrDI 1724 CATTGC GCAATG(2/0)
BssSI 873 CACGAG CACGAG(-5/-1)
BstAPI 2568 GCATTACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1681 CGCG CG^CG
DraI 1834 TTTAAA TTT^AAA
DrdI 424 GACTTGAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 361 CCTGTAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 418 ATGCAT ATGCA^T
FbaI 2201 TGATCA T^GATCA
MfeI 1192 CAATTG C^AATTG
MroI 1815 TCCGGA T^CCGGA
MslI 1909 CATGGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1192 CAATTG C^AATTG
MvnI 1681 CGCG CG^CG
NsiI 418 ATGCAT ATGCA^T
ScaI 2648 AGTACT AGT^ACT
SfiI 1032 GGCCAAACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspI 889 AATATT AAT^ATT
StuI 1040 AGGCCT AGG^CCT
Tsp45I 38 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 16 enzymes) #
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BglI 310 GCCCAAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1033 GCCAAACAGGC
BglII 939 AGATCT A^GATCT
1419 AGATCT
BmyI 1841 GTGCCC G[AGT]GC[ACT]^C
2364 GGGCAC
BsgI 1628 CTGCAC GTGCAG(16/14)
1853 CTGCAC
BsiYI 361 CCTGTAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2645 CCCAGTACTGG
BslI 361 CCTGTAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2645 CCCAGTACTGG
BsmI 306 GAATGC GAATGC(1/-1)
2096 GCATTC
Bsp1286I 1841 GTGCCC G[AGT]GC[ACT]^C
2364 GGGCAC
BspMI 296 ACCTGC ACCTGC(4/8)
2423 ACCTGC
EcoRV 46 GATATC GAT^ATC
1437 GATATC
HindIII 72 AAGCTT A^AGCTT
814 AAGCTT
NdeI 327 CATATG CA^TATG
926 CATATG
PleI 1084 GACTC GAGTC(4/5)
2580 GAGTC
PstI 1310 CTGCAG CTGCA^G
2615 CTGCAG
SmlI 61 CTTAAG C^T[CT][GA]AG
1025 CTCAAG
XcmI 1034 CCAAACAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2274 CCACATAGACTTTGG
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# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
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AcsI AfaI AluI AlwI AlwNI
ApoI Asp700I AvaII BbvI BcnI
BsaJI BsmAI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraIII EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MnlI
MseI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XhoII XmnI