Restriction map for pFN21AB9778
 (Circular) (Six-base) MAPSORT of: pFN21AB9778.seq  Check: 4587  from: 1  to: 7099



pFN21AB9778  7099 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:35  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   3462    3462 
   Size:       7099
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4909     278 
   Size:         53      83     186     936    3373    2468

  Fragments arranged by size: 

                3373    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2119    3496    4442    1937 
   Size:        182    1377     946    4594

  Fragments arranged by size: 

                4594    1377     946     182
 
Acc65I G'GTAC_C

Cuts at:   4420    4420 
   Size:       7099
 
AclI AA'CG_TT

Cuts at:   1563    5226    5599    1563 
   Size:       3663     373    3063

  Fragments arranged by size: 

                3663    3063     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2439    2527    3039    3362    5162    6546    6814    1632 
   Size:        807      88     512     323    1800    1384     268    1917

  Fragments arranged by size: 

                1917    1800    1384     807     512     323     268      88
            
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    6902
 
AflIII A'CryG_T

Cuts at:   1203    3890    5998    6975    1203 
   Size:       2687    2108     977    1327

  Fragments arranged by size: 

                2687    2108    1327     977
 
AgeI A'CCGG_T

Cuts at:   5930    5930 
   Size:       7099
 
AhdI GACnn_n'nnGTC

Cuts at:   5828    5828 
   Size:       7099
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       7099
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       7099
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3258    3290    3258 
   Size:         32    7067
 
AlwNI CAG_nnn'CTG

Cuts at:   2980    3017    4842    6414    2980 
   Size:         37    1825    1572    3665

  Fragments arranged by size: 

                3665    1825    1572      37
 
ApaLI G'TGCA_C

Cuts at:   2146    5156    6312    2146 
   Size:       3010    1156    2933

  Fragments arranged by size: 

                3010    2933    1156
 
ApoI r'AATT_y

Cuts at:   1493    2008    2152    2405    2734    3023    3642    3656    4298 
   Size:        515     144     253     329     289     619      14     642

Cuts at:   4298    4408    4634    4786    6895    1493 
   Size:        110     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     642     619     515     329     289     253
                 226     152     144     110      14
 
AseI AT'TA_AT

Cuts at:    160    1032    5653     160 
   Size:        872    4621    1606

  Fragments arranged by size: 

                4621    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       7099
 
AvaI C'yCGr_G

Cuts at:   1870    1943    4424    1870 
   Size:         73    2481    4545

  Fragments arranged by size: 

                4545    2481      73
 
BamHI G'GATC_C

Cuts at:   4429    4796    4429 
   Size:        367    6732
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    4420    5875     618 
   Size:        325     217     627    2633    1455    1842

  Fragments arranged by size: 

                2633    1842    1455     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    4418    1391 
   Size:       3027    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2427    3540     928 
   Size:        971     528    1113    4487

  Fragments arranged by size: 

                4487    1113     971     528
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    5290    5324    1903 
   Size:         34    3353      34    3678

  Fragments arranged by size: 

                3678    3353      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3781    4991    6201    3781 
   Size:       1210    1210    4679

  Fragments arranged by size: 

                4679    1210    1210
 
BclI T'GATC_A

Cuts at:   1548    2037    3114    1548 
   Size:        489    1077    5533

  Fragments arranged by size: 

                5533    1077     489
 
BfrBI ATG'CAT

Cuts at:   3125    3125 
   Size:       7099
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    5710     136 
   Size:        107     122      71     631     605    4038    1525

  Fragments arranged by size: 

                4038    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   2506    3819    7094    2506 
   Size:       1313    3275    2511

  Fragments arranged by size: 

                3275    2511    1313
 
BlpI GC'TnA_GC

Cuts at:   2607    3927    4516    2607 
   Size:       1320     589    5190

  Fragments arranged by size: 

                5190    1320     589
 
Bme1580I G_kGCm'C

Cuts at:   1790    2150    5160    6316    1790 
   Size:        360    3010    1156    2573

  Fragments arranged by size: 

                3010    2573    1156     360
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       7099
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    4830    5788     442 
   Size:        416      53      83     399      24    3413     958    1753

  Fragments arranged by size: 

                3413    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       7099
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2217    2730    2824    2880    5759    1239 
   Size:        978     513      94      56    2879    2579

  Fragments arranged by size: 

                2879    2579     978     513      94      56
 
Bpu10I CC'TnA_GC

Cuts at:   2612    2612 
   Size:       7099
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2306    5224    6089    6387    2306 
   Size:       2918     865     298    3018

  Fragments arranged by size: 

                3018    2918     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    5762     882 
   Size:        617    4263    2219

  Fragments arranged by size: 

                4263    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    4116    4890     493 
   Size:        711    2912     774    2702

  Fragments arranged by size: 

                2912    2702     774     711
 
BsaBI GATnn'nnATC

Cuts at:   2374    7093    2374 
   Size:       4719    2380
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    4906    5288    5923     275 
   Size:         53      83     186     936    3373     382     635    1451

  Fragments arranged by size: 

                3373    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   5532    5930    6204    6351    6762    5532 
   Size:        398     274     147     411    5869

  Fragments arranged by size: 

                5869     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    3258    3288    3582    3612    1178 
   Size:         30     165      30    1855      30     294      30    4665

  Fragments arranged by size: 

                4665    1855     294     165      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2539    1186 
   Size:       1353    5746
 
BseYI C'CCAG_C

Cuts at:   4044    6302    6798    4044 
   Size:       2258     496    4345

  Fragments arranged by size: 

                4345    2258     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2172    2626    3490    3970    1163 
   Size:        573     436     454     864     480    4292

  Fragments arranged by size: 

                4292     864     573     480     454     436
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    4549    5310    6338    1540 
   Size:        275     186    2548     761    1028    2301

  Fragments arranged by size: 

                2548    2301    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2150    2673    5160    5245    6316    2150 
   Size:        523    2487      85    1071    2933

  Fragments arranged by size: 

                2933    2487    1071     523      85
 
BsmI GAATG_Cn'

Cuts at:   2181    3404    3512    4324    4610    4703    2181 
   Size:       1223     108     812     286      93    4577

  Fragments arranged by size: 

                4577    1223     812     286     108      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2150    2673    4418    5160    5245    6316    1391 
   Size:        399     360     523    1745     742      85    1071    2174

  Fragments arranged by size: 

                2174    1745    1071     742     523     399     360      85
            
 
BspEI T'CCGG_A

Cuts at:   6762    6762 
   Size:       7099
 
BspHI T'CATG_A

Cuts at:   3436    4276    4988    6718    3436 
   Size:        840     712    1730    3817

  Fragments arranged by size: 

                3817    1730     840     712
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3462    3917    4454     844 
   Size:       2618     455     537    3489

  Fragments arranged by size: 

                3489    2618     537     455
 
BsrBI CCG'CTC

Cuts at:   1394    1823    4986    1394 
   Size:        429    3163    3507

  Fragments arranged by size: 

                3507    3163     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3106    3484    3832    5594    5768      65 
   Size:       1183    1858     378     348    1762     174    1396

  Fragments arranged by size: 

                1858    1762    1396    1183     378     348     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4048    5743    5930    1700 
   Size:        110    2238    1695     187    2869

  Fragments arranged by size: 

                2869    2238    1695     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    2925    3863      96 
   Size:       2829     938    3332

  Fragments arranged by size: 

                3332    2829     938
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       7099
 
BssSI C'ACGA_G

Cuts at:   5159    6171    5159 
   Size:       1012    6087
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    3660     794 
   Size:       2866    4233
 
BstBI TT'CG_AA

Cuts at:   5903    5903 
   Size:       7099
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       7099
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2025    2025 
   Size:       7099
 
BstYI r'GATC_y

Cuts at:   1972    2343    2506    3689    3819    3868    4429    4796    5181 
   Size:        371     163    1183     130      49     561     367     385

Cuts at:   5181    5198    6650    6736    6748    7094    1972 
   Size:         17    1452      86      12     346    1977

  Fragments arranged by size: 

                1977    1452    1183     561     385     371     367     346
                 163     130      86      49      17      12
 
BstZ17I GTA'TAC

Cuts at:   2120    2120 
   Size:       7099
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    6547
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    6360

  Fragments arranged by size: 

                6360     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3772    4615    5409    5429    3772 
   Size:        843     794      20    5442

  Fragments arranged by size: 

                5442     843     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       7099
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564    2089    2124    2340    2375     529 
   Size:         35    1525      35     216      35    5253

  Fragments arranged by size: 

                5253    1525     216      35      35      35
 
DraI TTT'AAA

Cuts at:   2088    3334    4403    2088 
   Size:       1246    1069    4784

  Fragments arranged by size: 

                4784    1246    1069
 
DraIII CAC_nnn'GTG

Cuts at:   2195    2195 
   Size:       7099
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    6106     809 
   Size:        507    4790    1802

  Fragments arranged by size: 

                4790    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    3829    4546    5435 
   Size:         54    1425      50     275      18    1999     717     889

Cuts at:   5435    7064       8 
   Size:       1629      43

  Fragments arranged by size: 

                1999    1629    1425     889     717     275      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    4546    1537 
   Size:        275    2734    4090

  Fragments arranged by size: 

                4090    2734     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3617    5029    1353 
   Size:       2264    1412    3423

  Fragments arranged by size: 

                3423    2264    1412
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    5686    6060    6206     387 
   Size:       1064     138    4097     374     146    1280

  Fragments arranged by size: 

                4097    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2217    2439    2527    2730    2824    2880    3039 
   Size:        393     585     222      88     203      94      56     159

Cuts at:   3039    3362    5162    5759    6546    6814    1239 
   Size:        323    1800     597     787     268    1524

  Fragments arranged by size: 

                1800    1524     787     597     585     393     323     268
                 222     203     159      94      88      56
 
EcoRI G'AATT_C

Cuts at:   4408    4786    4408 
   Size:        378    6721
 
FspI TGC'GCA

Cuts at:   5605    5605 
   Size:       7099
 
HaeII r_GCGC'y

Cuts at:   1678    6246    1678 
   Size:       4568    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3582    3614    4052    4084    5740 
   Size:         32     135      32    2010      32     438      32    1656

Cuts at:   5740    5772    5814    5846    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    2010    1656     438     135      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3526    4093    4443    4689     669 
   Size:        988     281    1588     567     350     246    3079

  Fragments arranged by size: 

                3079    1588     988     567     350     281     246
 
HindIII A'AGCT_T

Cuts at:    748    4259     748 
   Size:       3511    3588
 
HpaI GTT'AAC

Cuts at:   3526    4689    3526 
   Size:       1163    5936
 
KpnI G_GTAC'C

Cuts at:   4424    4424 
   Size:       7099
 
MfeI C'AATT_G

Cuts at:   4197    4698    4197 
   Size:        501    6598
 
MluI A'CGCG_T

Cuts at:   6975    6975 
   Size:       7099
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2371    2692    4078    6213    6397    1305 
   Size:       1066     321    1386    2135     184    2007

  Fragments arranged by size: 

                2135    2007    1386    1066     321     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    5277

  Fragments arranged by size: 

                5277    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2943    3457    3994    5057    5416    5575     518 
   Size:        648    1777     514     537    1063     359     159    2042

  Fragments arranged by size: 

                2042    1777    1063     648     537     514     359     159
            
 
MspA1I CmG'CkG

Cuts at:   1606    4515    5192    6340    6585    1606 
   Size:       2909     677    1148     245    2120

  Fragments arranged by size: 

                2909    2120    1148     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    6547
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       7099
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       7099
 
NotI GC'GGCC_GC

Cuts at:   4546    4546 
   Size:       7099
 
NsiI A_TGCA'T

Cuts at:   3127    3127 
   Size:       7099
 
NspI r_CATG'y

Cuts at:   1132    1229    3774    3987    4457    6002    1132 
   Size:         97    2545     213     470    1545    2229

  Fragments arranged by size: 

                2545    2229    1545     470     213      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       7099
 
PciI A'CATG_T

Cuts at:   5998    5998 
   Size:       7099
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2138    2753    2897    1108 
   Size:       1030     615     144    5310

  Fragments arranged by size: 

                5310    1030     615     144
 
PmeI GTTT'AAAC

Cuts at:   4403    4403 
   Size:       7099
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3258    3290    5168    5200    6681    6713    3258 
   Size:         32    1878      32    1481      32    3644

  Fragments arranged by size: 

                3644    1878    1481      32      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       7099
 
PsiI TTA'TAA

Cuts at:   4353    4669    4353 
   Size:        316    6783
 
PstI C_TGCA'G

Cuts at:    830    3554    3581    4451     830 
   Size:       2724      27     870    3478

  Fragments arranged by size: 

                3478    2724     870      27
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       7099
 
SalI G'TCGA_C

Cuts at:   1936    4441    1936 
   Size:       2505    4594
 
SbfI CC_TGCA'GG

Cuts at:   4451    4451 
   Size:       7099
 
ScaI AGT'ACT

Cuts at:   3796    5347    7059    3796 
   Size:       1551    1712    3836

  Fragments arranged by size: 

                3836    1712    1551
 
SfcI C'TryA_G

Cuts at:    826    1045    3550    3577    4447    5582    6263    6454     826 
   Size:        219    2505      27     870    1135     681     191    1471

  Fragments arranged by size: 

                2505    1471    1135     870     681     219     191      27
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       7099
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       7099
 
SmaI CCC'GGG

Cuts at:   1872    4426    1872 
   Size:       2554    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2321    5203    6104    6366     820 
   Size:        197     926     378    2882     901     262    1553

  Fragments arranged by size: 

                2882    1553     926     901     378     262     197
 
SnaBI TAC'GTA

Cuts at:    493    4116    4890     493 
   Size:       3623     774    2702

  Fragments arranged by size: 

                3623    2702     774
 
SpeI A'CTAG_T

Cuts at:    152    3791     152 
   Size:       3639    3460
 
SphI G_CATG'C

Cuts at:   1132    4457    1132 
   Size:       3325    3774
 
SspI AAT'ATT

Cuts at:      5      52    2995    3256    5023       5 
   Size:         47    2943     261    1767    2081

  Fragments arranged by size: 

                2943    2081    1767     261      47
 
StuI AGG'CCT

Cuts at:   1517    3343    1517 
   Size:       1826    5273
 
StyI C'CwwG_G

Cuts at:    513    1065    3913    4017    4538     513 
   Size:        552    2848     104     521    3074

  Fragments arranged by size: 

                3074    2848     552     521     104
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2130    5126    5143    5296    5481    5926     325 
   Size:       1255     550    2996      17     153     185     445    1498

  Fragments arranged by size: 

                2996    1498    1255     550     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2925    3381    3794 
   Size:        274      80      33      51    1443     948     456     413

Cuts at:   3794    3863    5345    7057      96 
   Size:         69    1482    1712     138

  Fragments arranged by size: 

                1712    1482    1443     948     456     413     274     138
                  80      69      51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2860    2892    7059    7091    2860 
   Size:         32    4167      32    2868

  Fragments arranged by size: 

                4167    2868      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    2904    1316 
   Size:       1588    5511
 
XbaI T'CTAG_A

Cuts at:   4435    4435 
   Size:       7099
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2139    2139 
   Size:       7099
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       7099
 
XmaI C'CCGG_G

Cuts at:   1870    4424    1870 
   Size:       2554    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3024    3247    3942    5228    3024 
   Size:        223     695    1286    4895

  Fragments arranged by size: 

                4895    1286     695     223
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4907     276 
   Size:         53      83     186     936    3373    2468

  Fragments arranged by size: 

                3373    2468     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI
     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  Eco57MI    EcoRI     FspI
    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI
     NsiI     NspI     PasI     PciI    PflMI     PmeI     PpiI    PshAI
     PsiI     PstI     PvuI     SalI     SbfI     ScaI     SfcI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI     BplI
    BsiWI    BsmBI   Bsu36I  EcoICRI    EcoNI EcoO109I    EcoRV     FalI
     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NruI     PacI
     PfoI     PmlI    PpuMI   PspOMI    PspXI     PsrI    PvuII    RsrII
     SacI    SacII    SanDI     SapI    SexAI     SfiI     SfoI     SrfI
     SwaI
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