Restriction map for
pFN21AB9770
(Circular) (Six-base) MAPSORT of: pFN21AB9770.seq Check: 2231 from: 1 to: 6091
pFN21AB9770 6091 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2522 2522
Size: 6091
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3901 278
Size: 53 83 186 936 2365 2468
Fragments arranged by size:
2468 2365 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2669 3434 1937
Size: 732 765 4594
Fragments arranged by size:
4594 765 732
Acc65I G'GTAC_C
Cuts at: 3412 3412
Size: 6091
AclI AA'CG_TT
Cuts at: 1563 4218 4591 1563
Size: 2655 373 3063
Fragments arranged by size:
3063 2655 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2248 4154 5538 5806 1632
Size: 616 1906 1384 268 1917
Fragments arranged by size:
1917 1906 1384 616 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5894
AflIII A'CryG_T
Cuts at: 1203 4990 5967 1203
Size: 3787 977 1327
Fragments arranged by size:
3787 1327 977
AgeI A'CCGG_T
Cuts at: 2559 4922 2559
Size: 2363 3728
AhdI GACnn_n'nnGTC
Cuts at: 4820 4820
Size: 6091
AleI CACnn'nnGTG
Cuts at: 1166 2045 2840 1166
Size: 879 795 4417
Fragments arranged by size:
4417 879 795
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6091
AlwNI CAG_nnn'CTG
Cuts at: 3834 5406 3834
Size: 1572 4519
ApaI G_GGCC'C
Cuts at: 2192 2225 2192
Size: 33 6058
ApaLI G'TGCA_C
Cuts at: 4148 5304 4148
Size: 1156 4935
ApoI r'AATT_y
Cuts at: 1493 3400 3626 3778 5887 1493
Size: 1907 226 152 2109 1697
Fragments arranged by size:
2109 1907 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4645 160
Size: 872 3613 1606
Fragments arranged by size:
3613 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
AvaI C'yCGr_G
Cuts at: 1870 1943 2134 3416 1870
Size: 73 191 1282 4545
Fragments arranged by size:
4545 1282 191 73
BamHI G'GATC_C
Cuts at: 2706 2859 2973 3421 3788 2706
Size: 153 114 448 367 5009
Fragments arranged by size:
5009 448 367 153 114
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2239 2562 3032 3373 3412
Size: 325 217 627 452 323 470 341 39
Cuts at: 3412 4867 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 470 452 341 325 323
217 39
BanII G_rGCy'C
Cuts at: 1391 2171 2192 2225 2736 3153 3294 3410 1391
Size: 780 21 33 511 417 141 116 4072
Fragments arranged by size:
4072 780 511 417 141 116 33 21
BbeI G_GCGC'C
Cuts at: 3036 3377 3036
Size: 341 5750
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5120
BbvCI CC'TCA_GC
Cuts at: 3179 3179
Size: 6091
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4282 4316 1903
Size: 34 2345 34 3678
Fragments arranged by size:
3678 2345 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3983 5193 3983
Size: 1210 4881
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6091
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2346 4702 136
Size: 107 122 71 631 605 674 2356 1525
Fragments arranged by size:
2356 1525 674 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6086 6086
Size: 6091
BlpI GC'TnA_GC
Cuts at: 2285 2718 3252 3508 2285
Size: 433 534 256 4868
Fragments arranged by size:
4868 534 433 256
Bme1580I G_kGCm'C
Cuts at: 1790 2192 2225 2244 4152 5308 1790
Size: 402 33 19 1908 1156 2573
Fragments arranged by size:
2573 1908 1156 402 33 19
BmgBI CAC'GTC
Cuts at: 1318 2998 1318
Size: 1680 4411
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3822 4780 442
Size: 416 53 83 399 24 2405 958 1753
Fragments arranged by size:
2405 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6091
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2732 3389 4751 1239
Size: 1493 657 1362 2579
Fragments arranged by size:
2579 1493 1362 657
Bpu10I CC'TnA_GC
Cuts at: 2165 3179 2165
Size: 1014 5077
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2339 4216 5081 5379 2339
Size: 1877 865 298 3051
Fragments arranged by size:
3051 1877 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2738 4754 882
Size: 617 1239 2016 2219
Fragments arranged by size:
2219 2016 1239 617
BsaAI yAC'GTr
Cuts at: 493 1204 2752 3882 493
Size: 711 1548 1130 2702
Fragments arranged by size:
2702 1548 1130 711
BsaBI GATnn'nnATC
Cuts at: 6085 6085
Size: 6091
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2349 3033 3374 3898
Size: 53 83 186 936 816 684 341 524
Cuts at: 3898 4280 4915 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 816 684 635 524 382 341
186 83 53
BsaWI w'CCGG_w
Cuts at: 2559 2646 3003 4524 4922 5196 5343 5754 2559
Size: 87 357 1521 398 274 147 411 2896
Fragments arranged by size:
2896 1521 411 398 357 274 147 87
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3050 3080 3125 3155 1178
Size: 30 165 30 1647 30 45 30 4114
Fragments arranged by size:
4114 1647 165 45 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2044 2419 2639 1186
Size: 858 375 220 4638
Fragments arranged by size:
4638 858 375 220
BseYI C'CCAG_C
Cuts at: 2280 2777 3103 5294 5790 2280
Size: 497 326 2191 496 2581
Fragments arranged by size:
2581 2191 497 496 326
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2087 2639 2978 1163
Size: 573 351 552 339 4276
Fragments arranged by size:
4276 573 552 351 339
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3165 3541 4302 5330 1540
Size: 275 186 1164 376 761 1028 2301
Fragments arranged by size:
2301 1164 1028 761 376 275 186
BsiHKAI G_wGCw'C
Cuts at: 2171 4152 4237 5308 2171
Size: 1981 85 1071 2954
Fragments arranged by size:
2954 1981 1071 85
BsmI GAATG_Cn'
Cuts at: 2146 3602 3695 2146
Size: 1456 93 4542
Fragments arranged by size:
4542 1456 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2171 2192 2225 2244 2736 3153 3294
Size: 399 381 21 33 19 492 417 141
Cuts at: 3294 3410 4152 4237 5308 1391
Size: 116 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 492 417 399 381 141
116 85 33 21 19
BspEI T'CCGG_A
Cuts at: 5754 5754
Size: 6091
BspHI T'CATG_A
Cuts at: 3980 5710 3980
Size: 1730 4361
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2522 3446 844
Size: 1678 924 3489
Fragments arranged by size:
3489 1678 924
BsrBI CCG'CTC
Cuts at: 1394 1823 2029 2427 2629 2952 3978 1394
Size: 429 206 398 202 323 1026 3507
Fragments arranged by size:
3507 1026 429 398 323 206 202
BsrDI GCAATG_nn'
Cuts at: 65 1248 4586 4760 65
Size: 1183 3338 174 1396
Fragments arranged by size:
3338 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2559 2695 3271 4735 4922 1700
Size: 110 749 136 576 1464 187 2869
Fragments arranged by size:
2869 1464 749 576 187 136 110
BsrGI T'GTAC_A
Cuts at: 96 2451 96
Size: 2355 3736
BssHII G'CGCG_C
Cuts at: 1925 3115 1925
Size: 1190 4901
BssSI C'ACGA_G
Cuts at: 4151 5163 4151
Size: 1012 5079
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6091
BstBI TT'CG_AA
Cuts at: 4895 4895
Size: 6091
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6091
BstYI r'GATC_y
Cuts at: 1972 2706 2859 2973 3421 3788 4173 4190 5642
Size: 734 153 114 448 367 385 17 1452
Cuts at: 5642 5728 5740 6086 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 734 448 385 367 346 153
114 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2374 2431 2374
Size: 57 6034
BtgI C'CryG_G
Cuts at: 513 1065 2004 2085 2217 2595 2727 513
Size: 552 939 81 132 378 132 3877
Fragments arranged by size:
3877 939 552 378 132 132 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2917 2941 487
Size: 683 56 1691 24 3637
Fragments arranged by size:
3637 1691 683 56 24
BtsI GCAGTG_nn'
Cuts at: 3607 4401 4421 3607
Size: 794 20 5277
Fragments arranged by size:
5277 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6091
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6056
DraI TTT'AAA
Cuts at: 3395 3395
Size: 6091
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2894 5098 809
Size: 507 1578 2204 1802
Fragments arranged by size:
2204 1802 1578 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2592 2724 2982
Size: 54 1425 50 275 18 762 132 258
Cuts at: 2982 3162 3538 4427 6056 8
Size: 180 376 889 1629 43
Fragments arranged by size:
1629 1425 889 762 376 275 258 180
132 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3162 3538 1537
Size: 275 1350 376 4090
Fragments arranged by size:
4090 1350 376 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3317 4021 1353
Size: 1964 704 3423
Fragments arranged by size:
3423 1964 704
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3254 4678 5052 5198 387
Size: 1064 138 1665 1424 374 146 1280
Fragments arranged by size:
1665 1424 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2169 2169
Size: 6091
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2248 2732 3389 4154 4751 5538 5806
Size: 393 616 484 657 765 597 787 268
Cuts at: 5806 1239
Size: 1524
Fragments arranged by size:
1524 787 765 657 616 597 484 393
268
EcoO109I rG'GnC_Cy
Cuts at: 2117 2222 2359 2500 2117
Size: 105 137 141 5708
Fragments arranged by size:
5708 141 137 105
EcoRI G'AATT_C
Cuts at: 3400 3778 3400
Size: 378 5713
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2494 2494
Size: 6091
FspI TGC'GCA
Cuts at: 2495 4597 2495
Size: 2102 3989
HaeII r_GCGC'y
Cuts at: 1678 2682 3015 3036 3179 3377 5238 1678
Size: 1004 333 21 143 198 1861 2531
Fragments arranged by size:
2531 1861 1004 333 198 143 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2414 2446 2651 2683 3050
Size: 32 135 32 842 32 205 32 367
Cuts at: 3050 3082 4732 4764 4806 4838 1373
Size: 32 1650 32 42 32 2626
Fragments arranged by size:
2626 1650 842 367 205 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2670 3435 3681 669
Size: 988 281 732 765 246 3079
Fragments arranged by size:
3079 988 765 732 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6091
HpaI GTT'AAC
Cuts at: 3681 3681
Size: 6091
KasI G'GCGC_C
Cuts at: 3032 3373 3032
Size: 341 5750
KpnI G_GTAC'C
Cuts at: 3416 3416
Size: 6091
MfeI C'AATT_G
Cuts at: 3690 3690
Size: 6091
MluI A'CGCG_T
Cuts at: 5967 5967
Size: 6091
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5205 5389 1305
Size: 3900 184 2007
Fragments arranged by size:
3900 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2594 2984 10
Size: 54 1425 343 762 390 3117
Fragments arranged by size:
3117 1425 762 390 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2045 2237 2600 2696 2840 2960 3086
Size: 648 879 192 363 96 144 120 126
Cuts at: 3086 4049 4408 4567 518
Size: 963 359 159 2042
Fragments arranged by size:
2042 963 879 648 363 359 192 159
144 126 120 96
MspA1I CmG'CkG
Cuts at: 1606 2143 2457 2554 3507 4184 5332 5577 1606
Size: 537 314 97 953 677 1148 245 2120
Fragments arranged by size:
2120 1148 953 677 537 314 245 97
NaeI GCC'GGC
Cuts at: 3273 3273
Size: 6091
NarI GG'CG_CC
Cuts at: 3033 3374 3033
Size: 341 5750
NcoI C'CATG_G
Cuts at: 513 1065 2004 2085 2217 2595 513
Size: 552 939 81 132 378 4009
Fragments arranged by size:
4009 939 552 378 132 81
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6091
NgoMIV G'CCGG_C
Cuts at: 3271 3271
Size: 6091
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6091
NotI GC'GGCC_GC
Cuts at: 3538 3538
Size: 6091
NspI r_CATG'y
Cuts at: 1132 1229 2695 3085 3449 4994 1132
Size: 97 1466 390 364 1545 2229
Fragments arranged by size:
2229 1545 1466 390 364 97
PasI CC'CwG_GG
Cuts at: 1348 2113 2257 2335 1348
Size: 765 144 78 5104
Fragments arranged by size:
5104 765 144 78
PciI A'CATG_T
Cuts at: 4990 4990
Size: 6091
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6091
PfoI T'CCnGG_A
Cuts at: 2709 2709
Size: 6091
PmeI GTTT'AAAC
Cuts at: 3395 3395
Size: 6091
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2765 2797 4160 4192 5673 5705 2765
Size: 32 1363 32 1481 32 3151
Fragments arranged by size:
3151 1481 1363 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2359 2500 2359
Size: 141 5950
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6091
PsiI TTA'TAA
Cuts at: 3661 3661
Size: 6091
PspOMI G'GGCC_C
Cuts at: 2188 2221 2188
Size: 33 6058
PstI C_TGCA'G
Cuts at: 830 3443 830
Size: 2613 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6091
PvuII CAG'CTG
Cuts at: 2143 2457 2143
Size: 314 5777
SacI G_AGCT'C
Cuts at: 2171 2171
Size: 6091
SalI G'TCGA_C
Cuts at: 1936 2668 3433 1936
Size: 732 765 4594
Fragments arranged by size:
4594 765 732
SbfI CC_TGCA'GG
Cuts at: 3443 3443
Size: 6091
ScaI AGT'ACT
Cuts at: 2570 4339 6051 2570
Size: 1769 1712 2610
Fragments arranged by size:
2610 1769 1712
SexAI A'CCwGG_T
Cuts at: 2892 2892
Size: 6091
SfcI C'TryA_G
Cuts at: 826 1045 3439 4574 5255 5446 826
Size: 219 2394 1135 681 191 1471
Fragments arranged by size:
2394 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 3034 3375 3034
Size: 341 5750
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6091
SmaI CCC'GGG
Cuts at: 1872 2136 3418 1872
Size: 264 1282 4545
Fragments arranged by size:
4545 1282 264
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2354 4195 5096 5358 820
Size: 197 926 411 1841 901 262 1553
Fragments arranged by size:
1841 1553 926 901 411 262 197
SnaBI TAC'GTA
Cuts at: 493 3882 493
Size: 3389 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6091
SphI G_CATG'C
Cuts at: 1132 3449 1132
Size: 2317 3774
SspI AAT'ATT
Cuts at: 5 52 4015 5
Size: 47 3963 2081
Fragments arranged by size:
3963 2081 47
StuI AGG'CCT
Cuts at: 1517 3209 1517
Size: 1692 4399
StyI C'CwwG_G
Cuts at: 513 1065 2004 2085 2217 2595 3144 3201 3530
Size: 552 939 81 132 378 549 57 329
Cuts at: 3530 513
Size: 3074
Fragments arranged by size:
3074 939 552 549 378 329 132 81
57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2057 2191 2266 2756 3355 4118 4135
Size: 1255 477 134 75 490 599 763 17
Cuts at: 4135 4288 4473 4918 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 763 599 490 477 445 185
153 134 75 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2451 2568 4337
Size: 274 80 33 51 1443 474 117 1769
Cuts at: 4337 6049 96
Size: 1712 138
Fragments arranged by size:
1769 1712 1443 474 274 138 117 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6051 6083 6051
Size: 32 6059
Tth111I GACn'n_nGTC
Cuts at: 1316 2795 2894 1316
Size: 1479 99 4513
Fragments arranged by size:
4513 1479 99
XbaI T'CTAG_A
Cuts at: 3427 3427
Size: 6091
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2182 2254 2182
Size: 72 6019
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6091
XmaI C'CCGG_G
Cuts at: 1870 2134 3416 1870
Size: 264 1282 4545
Fragments arranged by size:
4545 1282 264
XmnI GAAnn'nnTTC
Cuts at: 4220 4220
Size: 6091
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3899 276
Size: 53 83 186 936 2365 2468
Fragments arranged by size:
2468 2365 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FseI FspAI
NruI NsiI PacI PmlI PspXI PsrI RsrII SacII
SanDI SapI SfiI SrfI SwaI