(Circular) (Six-base) MAPSORT of: pFN21AB9762.seq Check: 9575 from: 1 to: 7105 pFN21AB9762 7105 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3972 4915 278 Size: 53 83 186 936 2436 943 2468 Fragments arranged by size: 2468 2436 943 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2760 3642 4448 1937 Size: 823 882 806 4594 Fragments arranged by size: 4594 882 823 806 Acc65I G'GTAC_C Cuts at: 3154 4426 3154 Size: 1272 5833 AclI AA'CG_TT Cuts at: 1563 2154 5232 5605 1563 Size: 591 3078 373 3063 Fragments arranged by size: 3078 3063 591 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2951 3640 3672 5168 6552 6820 1632 Size: 1319 689 32 1496 1384 268 1917 Fragments arranged by size: 1917 1496 1384 1319 689 268 32 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6908 AflIII A'CryG_T Cuts at: 1203 2742 6004 6981 1203 Size: 1539 3262 977 1327 Fragments arranged by size: 3262 1539 1327 977 AgeI A'CCGG_T Cuts at: 5936 5936 Size: 7105 AhdI GACnn_n'nnGTC Cuts at: 5834 5834 Size: 7105 AleI CACnn'nnGTG Cuts at: 1166 3428 1166 Size: 2262 4843 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7105 AlwNI CAG_nnn'CTG Cuts at: 2546 4271 4848 6420 2546 Size: 1725 577 1572 3231 Fragments arranged by size: 3231 1725 1572 577 ApaLI G'TGCA_C Cuts at: 2738 5162 6318 2738 Size: 2424 1156 3525 Fragments arranged by size: 3525 2424 1156 ApoI r'AATT_y Cuts at: 1493 2915 3916 4176 4282 4294 4414 4640 4792 Size: 1422 1001 260 106 12 120 226 152 Cuts at: 4792 6901 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1422 1001 260 226 152 120 106 12 AseI AT'TA_AT Cuts at: 160 1032 5659 160 Size: 872 4627 1606 Fragments arranged by size: 4627 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7105 AvaI C'yCGr_G Cuts at: 1870 1943 2401 4430 1870 Size: 73 458 2029 4545 Fragments arranged by size: 4545 2029 458 73 BamHI G'GATC_C Cuts at: 3236 4435 4802 3236 Size: 1199 367 5539 Fragments arranged by size: 5539 1199 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2245 3154 4426 5881 618 Size: 325 217 627 458 909 1272 1455 1842 Fragments arranged by size: 1842 1455 1272 909 627 458 325 217 BanII G_rGCy'C Cuts at: 1391 2393 2401 3941 4424 1391 Size: 1002 8 1540 483 4072 Fragments arranged by size: 4072 1540 1002 483 8 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2589 928 Size: 971 690 5444 Fragments arranged by size: 5444 971 690 BbvCI CC'TCA_GC Cuts at: 2427 2427 Size: 7105 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5296 5330 1903 Size: 34 3359 34 3678 Fragments arranged by size: 3678 3359 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4997 6207 4997 Size: 1210 5895 BclI T'GATC_A Cuts at: 1548 1548 Size: 7105 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2139 2345 Size: 107 122 71 631 605 337 130 206 Cuts at: 2345 5716 136 Size: 3371 1525 Fragments arranged by size: 3371 1525 631 605 337 206 130 122 107 71 BglII A'GATC_T Cuts at: 3538 7100 3538 Size: 3562 3543 BlpI GC'TnA_GC Cuts at: 4522 4522 Size: 7105 Bme1580I G_kGCm'C Cuts at: 1790 2250 2501 2742 5166 6322 1790 Size: 460 251 241 2424 1156 2573 Fragments arranged by size: 2573 2424 1156 460 251 241 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7105 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4281 4836 5794 Size: 416 53 83 399 24 2864 555 958 Cuts at: 5794 442 Size: 1753 Fragments arranged by size: 2864 1753 958 555 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7105 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2393 3483 5765 1239 Size: 1154 1090 2282 2579 Fragments arranged by size: 2579 2282 1154 1090 Bpu10I CC'TnA_GC Cuts at: 2427 2427 Size: 7105 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5230 6095 6393 5230 Size: 865 298 5942 Fragments arranged by size: 5942 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2882 3425 4241 5768 882 Size: 617 1383 543 816 1527 2219 Fragments arranged by size: 2219 1527 1383 816 617 543 BsaAI yAC'GTr Cuts at: 493 1204 4896 493 Size: 711 3692 2702 Fragments arranged by size: 3692 2702 711 BsaBI GATnn'nnATC Cuts at: 7099 7099 Size: 7105 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2162 2265 3969 4912 Size: 53 83 186 936 629 103 1704 943 Cuts at: 4912 5294 5929 275 Size: 382 635 1451 Fragments arranged by size: 1704 1451 943 936 635 629 382 186 103 83 53 BsaWI w'CCGG_w Cuts at: 2260 2393 3661 5538 5936 6210 6357 6768 2260 Size: 133 1268 1877 398 274 147 411 2597 Fragments arranged by size: 2597 1877 1268 411 398 274 147 133 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4021 4051 4295 4325 1178 Size: 30 165 30 2618 30 244 30 3958 Fragments arranged by size: 3958 2618 244 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2036 2390 1186 Size: 850 354 5901 Fragments arranged by size: 5901 850 354 BseYI C'CCAG_C Cuts at: 6308 6804 6308 Size: 496 6609 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6532 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2523 4555 5316 6344 1540 Size: 275 186 522 2032 761 1028 2301 Fragments arranged by size: 2301 2032 1028 761 522 275 186 BsiHKAI G_wGCw'C Cuts at: 2742 3012 5166 5251 6322 2742 Size: 270 2154 85 1071 3525 Fragments arranged by size: 3525 2154 1071 270 85 BsmI GAATG_Cn' Cuts at: 4616 4709 4616 Size: 93 7012 BsmBI CGTCTCn'nnnn_ Cuts at: 2530 2530 Size: 7105 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2250 2393 2401 2501 2742 3012 3941 Size: 399 460 143 8 100 241 270 929 Cuts at: 3941 4424 5166 5251 6322 1391 Size: 483 742 85 1071 2174 Fragments arranged by size: 2174 1071 929 742 483 460 399 270 241 143 100 85 8 BspEI T'CCGG_A Cuts at: 2260 2393 3661 6768 2260 Size: 133 1268 3107 2597 Fragments arranged by size: 3107 2597 1268 133 BspHI T'CATG_A Cuts at: 4994 6724 4994 Size: 1730 5375 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4460 844 Size: 3616 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4992 1394 Size: 429 3169 3507 Fragments arranged by size: 3507 3169 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5600 5774 65 Size: 1183 4352 174 1396 Fragments arranged by size: 4352 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2267 2420 3875 5749 5936 1700 Size: 110 457 153 1455 1874 187 2869 Fragments arranged by size: 2869 1874 1455 457 187 153 110 BsrGI T'GTAC_A Cuts at: 96 4345 96 Size: 4249 2856 BssHII G'CGCG_C Cuts at: 1925 2439 1925 Size: 514 6591 BssSI C'ACGA_G Cuts at: 5165 6177 5165 Size: 1012 6093 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7105 BstBI TT'CG_AA Cuts at: 2905 5909 2905 Size: 3004 4101 BstEII G'GTnAC_C Cuts at: 1184 2431 2455 1184 Size: 1247 24 5834 Fragments arranged by size: 5834 1247 24 BstYI r'GATC_y Cuts at: 1972 2632 3236 3256 3538 3993 4435 4802 5187 Size: 660 604 20 282 455 442 367 385 Cuts at: 5187 5204 6656 6742 6754 7100 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 660 604 455 442 385 367 346 282 86 20 17 12 Bsu36I CC'TnA_GG Cuts at: 2055 2539 2055 Size: 484 6621 BtgI C'CryG_G Cuts at: 513 1065 2004 2202 2517 2806 513 Size: 552 939 198 315 289 4812 Fragments arranged by size: 4812 939 552 315 289 198 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3080 487 Size: 683 56 1854 4512 Fragments arranged by size: 4512 1854 683 56 BtsI GCAGTG_nn' Cuts at: 2388 3423 3435 4621 5415 5435 2388 Size: 1035 12 1186 794 20 4058 Fragments arranged by size: 4058 1186 1035 794 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7105 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7070 DraI TTT'AAA Cuts at: 4409 4409 Size: 7105 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6112 809 Size: 507 4796 1802 Fragments arranged by size: 4796 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2298 3877 4552 Size: 54 1425 50 275 18 468 1579 675 Cuts at: 4552 5441 7070 8 Size: 889 1629 43 Fragments arranged by size: 1629 1579 1425 889 675 468 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4552 1537 Size: 275 2740 4090 Fragments arranged by size: 4090 2740 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2337 2951 3171 3294 3691 5035 1353 Size: 984 614 220 123 397 1344 3423 Fragments arranged by size: 3423 1344 984 614 397 220 123 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2033 2206 5692 6066 6212 387 Size: 1064 138 444 173 3486 374 146 1280 Fragments arranged by size: 3486 1280 1064 444 374 173 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2393 2951 3483 3640 3672 5168 5765 Size: 393 761 558 532 157 32 1496 597 Cuts at: 5765 6552 6820 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1496 787 761 597 558 532 393 268 157 32 EcoO109I rG'GnC_Cy Cuts at: 2190 2543 2190 Size: 353 6752 EcoRI G'AATT_C Cuts at: 4294 4414 4792 4294 Size: 120 378 6607 Fragments arranged by size: 6607 378 120 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2809 2809 Size: 7105 FspI TGC'GCA Cuts at: 5611 5611 Size: 7105 HaeII r_GCGC'y Cuts at: 1678 2015 2274 2351 2421 2427 2508 6252 1678 Size: 337 259 77 70 6 81 3744 2531 Fragments arranged by size: 3744 2531 337 259 81 77 70 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3545 3577 4021 4053 5746 Size: 32 135 32 1973 32 444 32 1693 Cuts at: 5746 5778 5820 5852 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1973 1693 444 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4449 4695 669 Size: 988 281 2511 246 3079 Fragments arranged by size: 3079 2511 988 281 246 HindIII A'AGCT_T Cuts at: 748 3512 748 Size: 2764 4341 HpaI GTT'AAC Cuts at: 4695 4695 Size: 7105 KpnI G_GTAC'C Cuts at: 3158 4430 3158 Size: 1272 5833 MfeI C'AATT_G Cuts at: 4704 4704 Size: 7105 MluI A'CGCG_T Cuts at: 6981 6981 Size: 7105 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2545 2877 3297 4096 6219 6403 1305 Size: 1240 332 420 799 2123 184 2007 Fragments arranged by size: 2123 2007 1240 799 420 332 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5283 Fragments arranged by size: 5283 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2775 3428 5063 5422 5581 518 Size: 648 1609 653 1635 359 159 2042 Fragments arranged by size: 2042 1635 1609 653 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2222 2255 4521 5198 6346 6591 1606 Size: 616 33 2266 677 1148 245 2120 Fragments arranged by size: 2266 2120 1148 677 616 245 33 NaeI GCC'GGC Cuts at: 2269 2422 3877 2269 Size: 153 1455 5497 Fragments arranged by size: 5497 1455 153 NcoI C'CATG_G Cuts at: 513 1065 2004 2806 513 Size: 552 939 802 4812 Fragments arranged by size: 4812 939 802 552 NdeI CA'TA_TG Cuts at: 387 3790 387 Size: 3403 3702 NgoMIV G'CCGG_C Cuts at: 2267 2420 3875 2267 Size: 153 1455 5497 Fragments arranged by size: 5497 1455 153 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7105 NotI GC'GGCC_GC Cuts at: 4552 4552 Size: 7105 NspI r_CATG'y Cuts at: 1132 1229 2746 4463 6008 1132 Size: 97 1517 1717 1545 2229 Fragments arranged by size: 2229 1717 1545 1517 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7105 PciI A'CATG_T Cuts at: 2742 6004 2742 Size: 3262 3843 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7105 PmeI GTTT'AAAC Cuts at: 4409 4409 Size: 7105 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5174 5206 6687 6719 5174 Size: 32 1481 32 5560 Fragments arranged by size: 5560 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2190 2543 2190 Size: 353 6752 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7105 PsiI TTA'TAA Cuts at: 4675 4675 Size: 7105 PstI C_TGCA'G Cuts at: 830 2310 3430 3946 4387 4457 830 Size: 1480 1120 516 441 70 3478 Fragments arranged by size: 3478 1480 1120 516 441 70 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7105 SalI G'TCGA_C Cuts at: 1936 4447 1936 Size: 2511 4594 SanDI GG'GwC_CC Cuts at: 2190 2190 Size: 7105 SbfI CC_TGCA'GG Cuts at: 4457 4457 Size: 7105 ScaI AGT'ACT Cuts at: 5353 7065 5353 Size: 1712 5393 SexAI A'CCwGG_T Cuts at: 4371 4371 Size: 7105 SfcI C'TryA_G Cuts at: 826 1045 2306 2656 3426 3843 3942 4059 4263 Size: 219 1261 350 770 417 99 117 204 Cuts at: 4263 4383 4453 5588 6269 6460 826 Size: 120 70 1135 681 191 1471 Fragments arranged by size: 1471 1261 1135 770 681 417 350 219 204 191 120 117 99 70 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7105 SgrAI Cr'CCGG_yG Cuts at: 1700 2267 2420 1700 Size: 567 153 6385 Fragments arranged by size: 6385 567 153 SmaI CCC'GGG Cuts at: 1872 2403 4432 1872 Size: 531 2029 4545 Fragments arranged by size: 4545 2029 531 SmlI C'TyrA_G Cuts at: 820 1017 1943 5209 6110 6372 820 Size: 197 926 3266 901 262 1553 Fragments arranged by size: 3266 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4896 493 Size: 4403 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7105 SphI G_CATG'C Cuts at: 1132 4463 1132 Size: 3331 3774 SspI AAT'ATT Cuts at: 5 52 3870 5029 5 Size: 47 3818 1159 2081 Fragments arranged by size: 3818 2081 1159 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7105 StyI C'CwwG_G Cuts at: 513 1065 2004 2806 3361 4544 513 Size: 552 939 802 555 1183 3074 Fragments arranged by size: 3074 1183 939 802 555 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2298 3520 3837 4265 5132 5149 5302 Size: 1255 718 1222 317 428 867 17 153 Cuts at: 5302 5487 5932 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1222 867 718 445 428 317 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2745 4345 5351 Size: 274 80 33 51 1443 768 1600 1006 Cuts at: 5351 7063 96 Size: 1712 138 Fragments arranged by size: 1712 1600 1443 1006 768 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7065 7097 7065 Size: 32 7073 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7105 XbaI T'CTAG_A Cuts at: 4441 4441 Size: 7105 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2203 2011 Size: 192 6913 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7105 XmaI C'CCGG_G Cuts at: 1870 2401 4430 1870 Size: 531 2029 4545 Fragments arranged by size: 4545 2029 531 XmnI GAAnn'nnTTC Cuts at: 2672 5234 2672 Size: 2562 4543 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3970 4913 276 Size: 53 83 186 936 2436 943 2468 Fragments arranged by size: 2468 2436 943 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI SfiI SfoI SrfI SwaI