(Circular) (Six-base) MAPSORT of: pFN21AB9735.seq Check: 8200 from: 1 to: 6154 pFN21AB9735 6154 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3964 278 Size: 53 83 186 936 2428 2468 Fragments arranged by size: 2468 2428 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2346 3497 1937 Size: 409 1151 4594 Fragments arranged by size: 4594 1151 409 Acc65I G'GTAC_C Cuts at: 3475 3475 Size: 6154 AclI AA'CG_TT Cuts at: 1563 4281 4654 1563 Size: 2718 373 3063 Fragments arranged by size: 3063 2718 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2198 2417 3363 4217 5601 5869 1632 Size: 566 219 946 854 1384 268 1917 Fragments arranged by size: 1917 1384 946 854 566 268 219 AfeI AGC'GCT Cuts at: 3014 3014 Size: 6154 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5957 AflIII A'CryG_T Cuts at: 1203 2289 5053 6030 1203 Size: 1086 2764 977 1327 Fragments arranged by size: 2764 1327 1086 977 AgeI A'CCGG_T Cuts at: 4985 4985 Size: 6154 AhdI GACnn_n'nnGTC Cuts at: 4883 4883 Size: 6154 AleI CACnn'nnGTG Cuts at: 1166 2858 1166 Size: 1692 4462 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2922 1184 Size: 1738 4416 AlwNI CAG_nnn'CTG Cuts at: 3178 3206 3281 3897 5469 3178 Size: 28 75 616 1572 3863 Fragments arranged by size: 3863 1572 616 75 28 ApaI G_GGCC'C Cuts at: 2283 2283 Size: 6154 ApaLI G'TGCA_C Cuts at: 4211 5367 4211 Size: 1156 4998 ApoI r'AATT_y Cuts at: 1493 2111 2234 3463 3689 3841 5950 1493 Size: 618 123 1229 226 152 2109 1697 Fragments arranged by size: 2109 1697 1229 618 226 152 123 AseI AT'TA_AT Cuts at: 160 1032 4708 160 Size: 872 3676 1606 Fragments arranged by size: 3676 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6154 AvaI C'yCGr_G Cuts at: 1870 1943 2038 3479 1870 Size: 73 95 1441 4545 Fragments arranged by size: 4545 1441 95 73 BamHI G'GATC_C Cuts at: 3484 3851 3484 Size: 367 5787 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2686 3475 4930 618 Size: 325 217 627 899 789 1455 1842 Fragments arranged by size: 1842 1455 899 789 627 325 217 BanII G_rGCy'C Cuts at: 1391 2283 3473 1391 Size: 892 1190 4072 Fragments arranged by size: 4072 1190 892 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5183 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4345 4379 1903 Size: 34 2408 34 3678 Fragments arranged by size: 3678 2408 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2714 4046 5256 2714 Size: 1332 1210 3612 Fragments arranged by size: 3612 1332 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6154 BfrBI ATG'CAT Cuts at: 2596 2596 Size: 6154 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4765 136 Size: 107 122 71 631 605 3093 1525 Fragments arranged by size: 3093 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6149 6149 Size: 6154 BlpI GC'TnA_GC Cuts at: 2943 3298 3571 2943 Size: 355 273 5526 Fragments arranged by size: 5526 355 273 Bme1580I G_kGCm'C Cuts at: 1790 2283 2691 4215 5371 1790 Size: 493 408 1524 1156 2573 Fragments arranged by size: 2573 1524 1156 493 408 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6154 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2970 3157 3194 Size: 416 53 83 399 24 1553 187 37 Cuts at: 3194 3433 3885 4843 442 Size: 239 452 958 1753 Fragments arranged by size: 1753 1553 958 452 416 399 239 187 83 53 37 24 BmtI G_CTAG'C Cuts at: 1056 2220 1056 Size: 1164 4990 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2365 2365 Size: 6154 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2622 3041 3258 4814 1239 Size: 1383 419 217 1556 2579 Fragments arranged by size: 2579 1556 1383 419 217 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3210 4279 5144 5442 3210 Size: 1069 865 298 3922 Fragments arranged by size: 3922 1069 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2571 2938 4817 882 Size: 617 1072 367 1879 2219 Fragments arranged by size: 2219 1879 1072 617 367 BsaAI yAC'GTr Cuts at: 493 1204 3945 493 Size: 711 2741 2702 Fragments arranged by size: 2741 2702 711 BsaBI GATnn'nnATC Cuts at: 2891 2991 6148 2891 Size: 100 3157 2897 Fragments arranged by size: 3157 2897 100 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3961 4343 4978 275 Size: 53 83 186 936 2428 382 635 1451 Fragments arranged by size: 2428 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4587 4985 5259 5406 5817 4587 Size: 398 274 147 411 4924 Fragments arranged by size: 4924 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2146 2176 2524 2554 2792 Size: 30 165 30 743 30 348 30 238 Cuts at: 2792 2822 3256 3286 1178 Size: 30 434 30 4046 Fragments arranged by size: 4046 743 434 348 238 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2204 2800 1186 Size: 1018 596 4540 Fragments arranged by size: 4540 1018 596 BseYI C'CCAG_C Cuts at: 2119 2276 2922 5357 5853 2119 Size: 157 646 2435 496 2420 Fragments arranged by size: 2435 2420 646 496 157 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3192 1163 Size: 573 1456 4125 Fragments arranged by size: 4125 1456 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3604 4365 5393 1540 Size: 275 186 1603 761 1028 2301 Fragments arranged by size: 2301 1603 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4215 4300 5371 4215 Size: 85 1071 4998 Fragments arranged by size: 4998 1071 85 BsmI GAATG_Cn' Cuts at: 3665 3758 3665 Size: 93 6061 BsmBI CGTCTCn'nnnn_ Cuts at: 2004 2004 Size: 6154 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2283 2691 3473 4215 4300 5371 1391 Size: 399 493 408 782 742 85 1071 2174 Fragments arranged by size: 2174 1071 782 742 493 408 399 85 BspEI T'CCGG_A Cuts at: 5817 5817 Size: 6154 BspHI T'CATG_A Cuts at: 3423 4043 5773 3423 Size: 620 1730 3804 Fragments arranged by size: 3804 1730 620 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3509 844 Size: 2665 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4041 1394 Size: 429 2218 3507 Fragments arranged by size: 3507 2218 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2038 2142 3157 3322 4649 4823 65 Size: 1183 790 104 1015 165 1327 174 1396 Fragments arranged by size: 1396 1327 1183 1015 790 174 165 104 BsrFI r'CCGG_y Cuts at: 1700 1810 4798 4985 1700 Size: 110 2988 187 2869 Fragments arranged by size: 2988 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6154 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6154 BssSI C'ACGA_G Cuts at: 4214 5226 4214 Size: 1012 5142 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6154 BstBI TT'CG_AA Cuts at: 4958 4958 Size: 6154 BstEII G'GTnAC_C Cuts at: 1184 2410 1184 Size: 1226 4928 BstXI CCAn_nnnn'nTGG Cuts at: 2857 2857 Size: 6154 BstYI r'GATC_y Cuts at: 1972 2992 3484 3851 4236 4253 5705 5791 5803 Size: 1020 492 367 385 17 1452 86 12 Cuts at: 5803 6149 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1020 492 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2682 2856 513 Size: 552 939 678 174 3811 Fragments arranged by size: 3811 939 678 552 174 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5415 Fragments arranged by size: 5415 683 56 BtsI GCAGTG_nn' Cuts at: 3204 3670 4464 4484 3204 Size: 466 794 20 4874 Fragments arranged by size: 4874 794 466 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6154 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6119 DraI TTT'AAA Cuts at: 3458 3458 Size: 6154 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3200 3433 5161 809 Size: 507 1884 233 1728 1802 Fragments arranged by size: 1884 1802 1728 507 233 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2667 2749 3351 Size: 54 1425 50 275 18 837 82 602 Cuts at: 3351 3375 3601 4490 6119 8 Size: 24 226 889 1629 43 Fragments arranged by size: 1629 1425 889 837 602 275 226 82 54 50 43 24 18 EagI C'GGCC_G Cuts at: 1537 1812 3601 1537 Size: 275 1789 4090 Fragments arranged by size: 4090 1789 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2207 2325 4084 1353 Size: 854 118 1759 3423 Fragments arranged by size: 3423 1759 854 118 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4741 5115 5261 387 Size: 1064 138 3152 374 146 1280 Fragments arranged by size: 3152 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2198 2417 2622 3041 3258 3363 4217 Size: 393 566 219 205 419 217 105 854 Cuts at: 4217 4814 5601 5869 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 854 787 597 566 419 393 268 219 217 205 105 EcoO109I rG'GnC_Cy Cuts at: 2280 2633 2280 Size: 353 5801 EcoRI G'AATT_C Cuts at: 2234 3463 3841 2234 Size: 1229 378 4547 Fragments arranged by size: 4547 1229 378 FspI TGC'GCA Cuts at: 4660 4660 Size: 6154 HaeII r_GCGC'y Cuts at: 1678 3016 5301 1678 Size: 1338 2285 2531 Fragments arranged by size: 2531 2285 1338 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2897 2929 4795 4827 4869 Size: 32 135 32 1325 32 1866 32 42 Cuts at: 4869 4901 1373 Size: 32 2626 Fragments arranged by size: 2626 1866 1325 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2722 3498 3744 669 Size: 988 281 784 776 246 3079 Fragments arranged by size: 3079 988 784 776 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6154 HpaI GTT'AAC Cuts at: 2722 3744 2722 Size: 1022 5132 KpnI G_GTAC'C Cuts at: 3479 3479 Size: 6154 MfeI C'AATT_G Cuts at: 3753 3753 Size: 6154 MluI A'CGCG_T Cuts at: 6030 6030 Size: 6154 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2189 2528 2574 5268 5452 1305 Size: 884 339 46 2694 184 2007 Fragments arranged by size: 2694 2007 884 339 184 46 MscI TGG'CCA Cuts at: 10 64 1489 1832 2669 2751 3353 3377 10 Size: 54 1425 343 837 82 602 24 2787 Fragments arranged by size: 2787 1425 837 602 343 82 54 24 MslI CAynn'nnrTG Cuts at: 518 1166 2094 2621 2858 2904 3371 4112 4471 Size: 648 928 527 237 46 467 741 359 Cuts at: 4471 4630 518 Size: 159 2042 Fragments arranged by size: 2042 928 741 648 527 467 359 237 159 46 MspA1I CmG'CkG Cuts at: 1606 3048 3124 3341 3570 4247 5395 5640 1606 Size: 1442 76 217 229 677 1148 245 2120 Fragments arranged by size: 2120 1442 1148 677 245 229 217 76 NcoI C'CATG_G Cuts at: 513 1065 2004 2682 2856 513 Size: 552 939 678 174 3811 Fragments arranged by size: 3811 939 678 552 174 NdeI CA'TA_TG Cuts at: 387 387 Size: 6154 NheI G'CTAG_C Cuts at: 1052 2216 1052 Size: 1164 4990 NotI GC'GGCC_GC Cuts at: 3601 3601 Size: 6154 NsiI A_TGCA'T Cuts at: 2598 2598 Size: 6154 NspI r_CATG'y Cuts at: 1132 1229 2293 3403 3419 3512 5057 1132 Size: 97 1064 1110 16 93 1545 2229 Fragments arranged by size: 2229 1545 1110 1064 97 93 16 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6154 PciI A'CATG_T Cuts at: 2289 5053 2289 Size: 2764 3390 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6154 PmeI GTTT'AAAC Cuts at: 3458 3458 Size: 6154 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4223 4255 5736 5768 4223 Size: 32 1481 32 4609 Fragments arranged by size: 4609 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2677 1613 Size: 1064 5090 PsiI TTA'TAA Cuts at: 3724 3724 Size: 6154 PspOMI G'GGCC_C Cuts at: 2279 2279 Size: 6154 PstI C_TGCA'G Cuts at: 830 2251 3040 3340 3506 830 Size: 1421 789 300 166 3478 Fragments arranged by size: 3478 1421 789 300 166 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6154 PvuII CAG'CTG Cuts at: 3124 3341 3124 Size: 217 5937 SalI G'TCGA_C Cuts at: 1936 3496 1936 Size: 1560 4594 SapI GCTCTTCn'nnn_ Cuts at: 2325 2325 Size: 6154 SbfI CC_TGCA'GG Cuts at: 3506 3506 Size: 6154 ScaI AGT'ACT Cuts at: 2959 4402 6114 2959 Size: 1443 1712 2999 Fragments arranged by size: 2999 1712 1443 SfcI C'TryA_G Cuts at: 826 1045 2247 3036 3212 3336 3502 4637 5318 Size: 219 1202 789 176 124 166 1135 681 Cuts at: 5318 5509 826 Size: 191 1471 Fragments arranged by size: 1471 1202 1135 789 681 219 191 176 166 124 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6154 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6154 SmaI CCC'GGG Cuts at: 1872 2040 3481 1872 Size: 168 1441 4545 Fragments arranged by size: 4545 1441 168 SmlI C'TyrA_G Cuts at: 820 1017 1943 3225 4258 5159 5421 820 Size: 197 926 1282 1033 901 262 1553 Fragments arranged by size: 1553 1282 1033 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3945 493 Size: 3452 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6154 SphI G_CATG'C Cuts at: 1132 3403 3512 1132 Size: 2271 109 3774 Fragments arranged by size: 3774 2271 109 SspI AAT'ATT Cuts at: 5 52 4078 5 Size: 47 4026 2081 Fragments arranged by size: 4026 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6154 StyI C'CwwG_G Cuts at: 513 1065 2004 2682 2856 3246 3593 513 Size: 552 939 678 174 390 347 3074 Fragments arranged by size: 3074 939 678 552 390 347 174 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2461 3061 3349 4181 4198 4351 4536 Size: 1255 881 600 288 832 17 153 185 Cuts at: 4536 4981 325 Size: 445 1498 Fragments arranged by size: 1498 1255 881 832 600 445 288 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2641 2957 4400 Size: 274 80 33 51 1443 664 316 1443 Cuts at: 4400 6112 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1443 664 316 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2524 2556 2792 2823 2824 2855 6114 6146 2524 Size: 32 236 31 1 31 3259 32 2532 Fragments arranged by size: 3259 2532 236 32 32 31 31 1 Tth111I GACn'n_nGTC Cuts at: 1316 2135 1316 Size: 819 5335 XbaI T'CTAG_A Cuts at: 3490 3490 Size: 6154 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2557 2557 Size: 6154 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6154 XmaI C'CCGG_G Cuts at: 1870 2038 3479 1870 Size: 168 1441 4545 Fragments arranged by size: 4545 1441 168 XmnI GAAnn'nnTTC Cuts at: 4283 4283 Size: 6154 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3962 276 Size: 53 83 186 936 2428 2468 Fragments arranged by size: 2468 2428 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI